Rnai approach for crop pest protection

ABSTRACT

Provided herein is the identification of insect RNAi target genes (IRTG) involved in gut microbial clearance and containment and examples of a novel biotechnology for devising pesticidal RNAi approaches.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application claims priority to PCT Application No.PCT/US2018/030506, filed May 1, 2018, which claims the benefit of U.S.Provisional Application No. 62/492,556, filed May 1, 2017, thedisclosures of which are incorporated herein by reference.

REFERENCE TO A SEQUENCE LISTING

This application contains a Sequence Listing that has been submitted inASCII format view EFS-Web and is hereby incorporated by reference in itsentirety. The ASCII copy is named “DDPSC0081-401-PC Sequence Listingfiled with Response to Correct Defects”, and is 191 kilobytes in size.

BACKGROUND

Insect pests are detrimental to crop production and human healththroughout the world and insect control can in some instances consumebetween 10-25% of a country's gross national product (GNP). (http WorldWide Web internet site “fao.org/3/a-av013e.pdf”). In the U.S., annualloss due to crop pests is estimated to exceed $120 billion USD/year.(Polaszek A. (1998) Wallingford, UK: CABI. 530 pp.).

Within crop pests, Lepidoptera are the most detrimental insect pests ofcereal crop cultivation. Chemical control is often expensive,inefficient, and can be associated with negative environmentalconsequences. Host plant resistance is an attractive option but impededby lack of robust Lepidoptera resistant germplasm (http World Wide Webinternet site“cnbc.com/2015/05/08/insects-feast-on-plants-endangering-crops-and-costing-billions.html”).

Since 1996, commercialization of crop plants genetically engineered toproduce Bacillus thuringiensis (Bt) insecticidal proteins have resultedin efficient pest control, increased yield, reduced insecticidal use,and enhanced farmer profits. (Khan Z R, et al. (2014). Philos. Trans. RSoc. Lond Biol Sci. 369: 1639).

Consequently, the cumulative area planted with Bt crops worldwidereached greater than 1 billion acres during 2011. Within the U.S., BtCorn, Bt Soybean, and Bt Cotton accounted for 90% of all the total corn,soybean and cotton acres during 2013 (Tabashnik B E, et al. (2013). Nat.Biotech. 31: 510-521). However, evolution of field resistance against Btin lepidopteran pests raises potential concerns about the sustainabilityof this approach. (Campagne P., et al. (2013) PLoS ONE 8(7): e69675.Doi:10.1371). That is further exacerbated by loss of resistance againstpyramided Bt traits as well (https World Wide Web internet site dtnpf.com/agriculture/web/ag/news/article/2016/08/10/rootworm-resistance-pyramided-bt.html).

SUMMARY

Provided herein is an isolated double stranded RNA (dsRNA) moleculecomprising a nucleic acid sequence complementary to about 200 to 1000contiguous nucleotides of a target gene sequence—wherein the target geneis a MIGGS-IRTG as defined herein—involved in gut microbe clearanceand/or containment induced by microbes ingested during feeding and/oractive feeding. In certain aspects, the target gene is critical forinsect immune responses and certain aspects provide that it isabundantly expressed in the midgut. In certain aspects, the target genesequence includes at least one of the protein coding region, the 5′ UTRregion, the 3′ UTR region, and any combination thereof, of a targetgene. Further, certain aspects provide that the dsRNA molecule silencesthe target gene when ingested by an insect. In certain aspects, thetarget gene is a type 1 MIGGS RNAi target or a type 2 MIGGS RNAi targetas defined elsewhere herein. In certain aspects, the target gene is apattern recognition receptor (PRR) class gene or an insect midgutstructural component gene. In certain aspects, the target gene isexpressed abundantly in a midgut specific manner during active feeding.

In certain aspects, a dsRNA molecule disclosed anywhere herein comprisestwo annealed complementary RNA strands. In certain aspects, said dsRNAmolecule comprises a single RNA strand comprising an inversely repeatedsequence with a spacer in between, wherein the single RNA strand cananneal to itself to form a hairpin loop structure.

In certain aspects, a dsRNA molecule disclosed anywhere herein comprisesa nucleic acid sequence complementary to about 200 to 1000 contiguousnucleotides of the protein coding region of the target gene sequence. Incertain aspects, said dsRNA molecule comprises a nucleic acid sequencecomplementary to about 200 to 1000 contiguous nucleotides of the 5′ UTRregion or the 3′ UTR region of the target gene sequence. In certainaspects, said dsRNA molecule comprises a nucleic acid sequencecomplementary to a contiguous region comprising at least about 50%, 60%,70%, 80%, 90%, 95%, 98%, 99%, or 100% of the length of the target genesequence protein coding region, 5′ UTR region, or 3′ UTR region. Incertain aspects, said dsRNA molecule comprises a nucleic acid sequencecomplementary to about 200 to 650 contiguous nucleotides of a targetgene sequence.

Certain aspects of this disclosure are drawn to a target gene selectedfrom the group consisting of M. sexta-Hemolin (MsHEM), M. sexta-Serineproteinase homolog 3 (MsSPH-3), M. sexta-Peptidoglycan recognitionprotein 2 (MsPGRP2), M. sexta-Beta-1, 3-glycan-recognition protein 2(MsβGRP2), M. sexta-Relish family protein 2A (MsREL2A), M. sexta-Dorsal(MsDor), M. sexta-Spätzle (MsSPZ1A), M. sexta-Toll receptor (MsTOLL), M.sexta-Scolexin A (MsSCA1), M. sexta-Hemolymph proteinase 18 (MsHP18), M.sexta-Transferrin (MsTRN), M. sexta-Arylphorin beta subunit (MsARP), M.sexta-Chymotrypsinogen-like protein 1 (MsCTL1), M. sexta-Valine RichMidgut Protein (MsVMP1), M. sexta-Imd (MsImd), M. sexta-FADD (MsFADD),M. sexta-Dredd (MsDRD), M. sexta-Relish F (MsReIF), M. sexta-Cdc42(MsCdc42), M. sexta-Dsor1 (MsDsor1), M. sexta-Fos (MsFos), M. sexta-Jra(MsJra), M. sexta-Caudal (MsCAD1), M. sexta-Atg8 (MsAtg8), M.sexta-Atg13 (MsAtg13), M. sexta-IAP1 (MsIAP1), M. sexta-Chitin synthase2 (MsChs2), M. sexta-Beta-1 tubulin (MsβTub), M. sexta-Betafructofuranosidase 1 (MsSuc1), and orthologs thereof. In certainaspects, the target gene is selected from the group consisting of M.sexta-Hemolin (MsHEM), M. sexta-Serine proteinase homolog 3 (MsSPH-3),M. sexta-Peptidoglycan recognition protein 2 (MsPGRP2), M. sexta-Beta-1,3-glycan-recognition protein 2 (MsβGRP2), M. sexta-Relish family protein2A (MsREL2A), M. sexta-Dorsal (MsDor), M. sexta-Spätzle (MsSPZ1A), M.sexta-Toll receptor (MsTOLL), M. sexta-Scolexin A (MsSCA1), M.sexta-Hemolymph proteinase 18 (MsHP18), M. sexta-Transferrin (MsTRN), M.sexta-Arylphorin beta subunit (MsARP), M. sexta-Chymotrypsinogen-likeprotein 1 (MsCTL1), M. sexta-Valine Rich Midgut Protein (MsVMP1), M.sexta-Imd (MsImd), M. sexta-FADD (MsFADD), M. sexta-Dredd (MsDRD), M.sexta-Relish F (MsReIF), M. sexta-Cdc42 (MsCdc42), M. sexta-Dsor1(MsDsor1), M. sexta-Fos (MsFos), M. sexta-Jra (MsJra), M. sexta-Caudal(MsCAD1), M. sexta-Atg8 (MsAtg8), M. sexta-Atg13 (MsAtg13), M.sexta-IAP1 (MsIAP1), M. sexta-Chitin synthase 2 (MsChs2), M.sexta-Beta-1 tubulin (MsβTub) and M. sexta-Beta fructofuranosidase 1(MsSuc1).

Also provided herein is an isolated double stranded RNA (dsRNA) moleculecomprising a nucleic acid sequence complementary to about 200 to 1000contiguous nucleotides of a target gene sequence, wherein the targetgene comprises a nucleic acid sequence selected from the groupconsisting of SEQ ID NOs: 1-14, 16-29, 31-70, 71-75, 76-88, 89-105, and106-110. In certain aspects, the target gene sequence includes at leastone of the protein coding region, the 5′ UTR region, the 3′ UTR region,and any combination thereof, of a target gene. Further, certain aspectsprovide that the dsRNA molecule silences the target gene when ingestedby an insect.

In certain aspects disclosed herein, the target gene is sequenceselected from the group consisting of: i) SEQ ID NOs: 1-9, 11, 14, 31,39, 43, 44, and 71-75.

In certain aspects disclosed herein, the target gene is sequenceselected from the group consisting of: ii) SEQ ID NOs: 3, 4, and 43. Incertain aspects, the dsRNA molecule causes impeded growth, developmentalprogression, and/or mortality and the like of TH, DMB, and FAW in anorthologous manner.

In certain aspects disclosed herein, the target gene is sequenceselected from the group consisting of: iii) SEQ ID NOs: 76, 77, 80, 81,85, 87, and 88. In certain aspects, the dsRNA molecule causes impededgrowth, developmental progression, and/or mortality and the like of DBM.Further, in certain aspects, the DBM is a Bt resistant strain.

In certain aspects disclosed herein, the target gene is sequenceselected from the group consisting of: iv) SEQ ID NOs: 89, 92, 96, 101,103, and 105. In certain aspects, the dsRNA molecule causes impededgrowth, developmental progression, and/or mortality and the like of FAW.

In certain aspects disclosed herein, the target gene is sequenceselected from the group consisting of: v) SEQ ID NOs: 107-110. Incertain aspects, the dsRNA molecule caused impeded growth, developmentalprogression, and/or mortality and the like of RFB.

In certain aspects of the dsRNA molecule disclosed above, the dsRNAmolecule comprises two annealed complementary RNA stands. In certainaspects, the dsRNA molecule comprises a single RNA strand comprising aninversely repeated sequence with a spacer in between and where thesingle RNA strand can anneal to itself to form a hairpin loop structure.

In certain of the dsRNA molecule disclosed above, the dsRNA moleculecomprises a nucleic acid sequence complementary to about 200 to 1000contiguous nucleotides of the protein coding region of the target genesequence. In certain aspects, the dsRNA molecule comprises a nucleicacid sequence complementary to a contiguous region comprising at leastabout 50%, 60%, 70%, 80%, 90%, 95%, 98%, 99%, or 100% of the length of asequence selected from the group consisting of SEQ ID NOs: 1-14, 16-29,31-70, 71-75, 76-88, 89-105, and 106-110. In certain aspects, the dsRNAmolecule comprises a nucleic acid sequence complementary to a contiguousregion comprising at least about 50%, 60%, 70%, 80%, 90%, 95%, 98%, 99%,or 100% of the length of the target gene sequence protein coding region,5′ UTR region, or 3′ UTR region.

In certain aspects disclosed herein the dsRNA molecule comprises anucleic acid sequence selected from the group consisting of SEQ ID NOs:111-119, 120-126, 127-135, and 136-139. In certain aspects, the dsRNA isa fragment of at least about 200 nucleotides thereof. In certainaspects, i) the isolated dsRNA molecule comprising a nucleic acidsequence selected from the group consisting of SEQ ID NOs: 110-119, orthe fragment thereof, causes impeded growth, developmental progression,and/or mortality and the like of TH; ii) the isolated dsRNA moleculecomprising a nucleic acid sequence selected from the group consisting ofSEQ ID NOs: 120-126, or the fragment thereof, causes impeded growth,developmental progression, and/or mortality and the like of DBM; iii)the isolated dsRNA molecule comprising a nucleic acid sequence selectedfrom the group consisting of SEQ ID NOs: 127-135, or the fragmentthereof, causes impeded growth, developmental progression, and/ormortality and the like of FAW; or iv) the isolated dsRNA moleculecomprising a nucleic acid sequence selected from the group consisting ofSEQ ID NOs: 136-139, or the fragment thereof, causes impeded growth,developmental progression, and/or mortality and the like of RFB.

In certain of any of the above aspects, the dsRNA molecule can formsiRNA. Certain aspects provide for an isolated siRNA molecule derivedfrom the processing of said dsRNA molecule.

Certain further aspects provide of an insecticidal compositioncomprising an isolated dsRNA molecule or an siRNA molecule disclosedanywhere herein, and a synthetic carrier or microbial conduit. Incertain aspects, a microorganism has a natural capacity or is engineeredto produce and/or deliver dsRNA to increase its bioavailability and/orbiostability for causing RNA interference including but not restrictedto plant growth promoting organisms, normal commensal and/or symbioticmicroorganisms associated with the target insect pest or parasitesand/or natural enemies of the target pest or pest target host or hostcultivation range etc. from an insect or parasite and/or natural enemiesof the target pest engineered or identified from natural populationscontaining microbial conduit to produce and/or deliver dsRNA and/ordrive the transmission of such microbial conduits into naturalpopulations of insect pests as a control option. In certain aspects ofan insecticidal composition disclosed herein, the dsRNA molecule isconjugated with the synthetic carrier.

Certain aspects are also drawn to a recombinant DNA construct encoding adsRNA molecule disclose anywhere herein. In certain aspects, therecombinant DNA construct comprising a gene silencing sequencecomprising about 200 to 1000 contiguous nucleotides of a target genesequence. In certain aspects, the target gene is a MIGGS-IRTG, asdefined herein, involved in gut microbe clearance and/or containmentinduced by microbes ingested during feeding and/or active feeding. Incertain aspects, the target gene is critical for insect immuneresponses. In certain aspects, the target gene is abundantly expressedin the midgut. In certain aspects, said target gene sequence includes atleast one of the protein coding region, the 5′ UTR region, the 3′ UTRregion, and any combination thereof, of a target gene. In certainaspects, the target gene is a type I MIGGS RNAi target or a type 2 MIGGSRNAi target as described elsewhere herein. In certain aspects, thetarget gene is a pattern recognition receptor (PRR) class gene or aninsect midgut structural component gene. In certain aspects, the targetgene is expressed abundantly in a midgut specific manner during activefeeding.

In any of the above aspects of a recombinant DNA construct, the genesilencing sequence comprises about 200 to 1000 contiguous nucleotides ofthe protein coding region of the target gene sequence. In certainaspects, the gene silencing sequence comprises about 200 to 1000contiguous nucleotides of the 5′ UTR region or the 3′ UTR region of thetarget gene sequence. In certain aspects, the gene silencing sequencecomprises at least 50%, 60%, 70%, 80%, 90%, 95%, 98%, 99%, or 100%contiguously of the length of target gene sequence protein codingregion, 5′ UTR region, or 3′ UTR region. In certain aspects, the genesilencing sequence comprises about 200 to 650 contiguous nucleotides ofthe target gene sequence.

In any of the above aspects of a recombinant DNA construct, and as notedthroughout this disclosure, in certain aspects, a target gene can beselected from the group consisting of M. sexta-Hemolin (MsHEM), M.sexta-Serine proteinase homolog 3 (MsSPH-3), M. sexta-Peptidoglycanrecognition protein 2 (MsPGRP2), M. sexta-Beta-1, 3-glycan-recognitionprotein 2 (MsβGRP2), M. sexta-Relish family protein 2A (MsREL2A), M.sexta-Dorsal (MsDor), M. sexta-Spätzle (MsSPZ1A), M. sexta-Toll receptor(MsTOLL), M. sexta-Scolexin A (MsSCA1), M. sexta-Hemolymph proteinase 18(MsHP18), M. sexta-Transferrin (MsTRN), M. sexta-Arylphorin beta subunit(MsARP), M. sexta-Chymotrypsinogen-like protein 1 (MsCTL1), M.sexta-Valine Rich Midgut Protein (MsVMP1), M. sexta-Imd (MsImd), M.sexta-FADD (MsFADD), M. sexta-Dredd (MsDRD), M. sexta-Relish F (MsReIF),M. sexta-Cdc42 (MsCdc42), M. sexta-Dsor1 (MsDsor1), M. sexta-Fos(MsFos), M. sexta-Jra (MsJra), M. sexta-Caudal (MsCAD1), M. sexta-Atg8(MsAtg8), M. sexta-Atg13 (MsAtg13), M. sexta-IAP1 (MsIAP1), M.sexta-Chitin synthase 2 (MsChs2), M. sexta-Beta-1 tubulin (MsβTub), M.sexta-Beta fructofuranosidase 1 (MsSuc1), and orthologs thereof. Incertain aspects a target gene can be selected from the group consistingof M. sexta-Hemolin (MsHEM), M. sexta-Serine proteinase homolog 3(MsSPH-3), M. sexta-Peptidoglycan recognition protein 2 (MsPGRP2), M.sexta-Beta-1, 3-glycan-recognition protein 2 (MsβGRP2), M. sexta-Relishfamily protein 2A (MsREL2A), M. sexta-Dorsal (MsDor), M. sexta-Spätzle(MsSPZ1A), M. sexta-Toll receptor (MsTOLL), M. sexta-Scolexin A(MsSCA1), M. sexta-Hemolymph proteinase 18 (MsHP18), M.sexta-Transferrin (MsTRN), M. sexta-Arylphorin beta subunit (MsARP), M.sexta-Chymotrypsinogen-like protein 1 (MsCTL1), M. sexta-Valine RichMidgut Protein (MsVMP1), M. sexta-Imd (MsImd), M. sexta-FADD (MsFADD),M. sexta-Dredd (MsDRD), M. sexta-Relish F (MsReIF), M. sexta-Cdc42(MsCdc42), M. sexta-Dsor1 (MsDsor1), M. sexta-Fos (MsFos), M. sexta-Jra(MsJra), M. sexta-Caudal (MsCAD1), M. sexta-Atg8 (MsAtg8), M.sexta-Atg13 (MsAtg13), M. sexta-IAP1 (MsIAP1), M. sexta-Chitin synthase2 (MsChs2), M. sexta-Beta-1 tubulin (MsβTub) and M. sexta-Betafructofuranosidase 1 (MsSuc1).

Further aspects provide for a recombinant DNA construct comprising agene silencing sequence comprising about 200 to 1000 contiguousnucleotides of a target gene sequence, wherein the target gene comprisesa nucleic acid sequence selected from the group consisting of SEQ IDNOs: 1-14, 16-29, 31-70, 71-75, 76-88, 89-105, and 106-110. In certainaspects, the target gene sequence includes at least one of the proteincoding region, the 5′ UTR region, the 3′ UTR region, and any combinationthereof, of a target gene.

In any of the above aspects of a recombinant DNA construct, the targetgene sequence is selected from the group consisting of: i) SEQ ID NOs:1-9, 11, 14, 31, 39, 43, 44, and 71-75; ii) SEQ ID NOs: 3, 4, and 43;iii) SEQ ID NOs: 76, 77, 80, 81, 85, 87, and 88; iv) SEQ ID NOs: 89, 92,96, 101, 103, and 105; and v) SEQ ID NOs: 107-109, and 110. In certainaspects, the gene silencing sequence comprises about 200 to 1000contiguous nucleotides of a sequence selected from the group consistingof SEQ ID NOs: 1-14, 16-29, 31-70, 71-75, 76-88, 89-105, and 106-110. Incertain aspects, the gene silencing sequence comprises at least 50%,60%, 70%, 80%, 90%, 95%, 98%, 99%, or 100% contiguously of a sequenceselected from the group consisting of SEQ ID Nos: 1-14, 16-29, 31-70,71-75, 76-88, 89-105, and 106-110. In certain aspects, the genesilencing sequence comprises about 200 to 650 contiguous nucleotides ofa sequence selected from the group consisting of SEQ ID NOs: 1-14,16-29, 31-70, 71-75, 76-88, 89-105, and 106-110. In certain aspects, thegene silencing sequence is operably linked to one or more promoters forthe expression of a dsRNA molecule that silences the target gene wheningested by an insect. In certain aspects, the construct is anexpression vector. And, in certain aspects, the expression vector cantarget single or multiple insect RNAi target genes or chimeric RNAitarget genes.

Certain aspects of the disclosure also provide for a host cellcomprising the dsRNA molecule, the siRNA molecule, a polynucleotideencoding a dsRNA molecule, and/or the construct or a dsRNA encodingsegment thereof disclose anywhere herein. In certain aspects, the hostcell is a bacterial or plant cell or organelle. In certain aspects, theorganelle is a plastid. In certain aspects, the host cell is atransgenic and/or transplastomic plant cell. In certain aspects, thehose cell expresses a dsRNA and/or produces an siRNA disclosed anywhereherein.

Certain aspects also provide for a transgenic and/or transplastomicplant comprising a dsRNA molecule, an siRNA molecule, a polynucleotideencoding the dsRNA, and/or a construct or a dsRNA encoding segmentdisclosed anywhere herein. In certain aspects, at least one cell of theplant expresses the dsRNA molecule and/or produces the siRNA molecule.Further, certain aspects provide for a seed, part, tissue, cell, ororganelle of the above transgenic and/or transplastomic plant. Incertain aspects, the seed, part, tissue, cell, or organelle comprisesthe dsRNA molecule and/or the siRNA molecule. In certain aspects, theorganelle is a plastid.

Certain aspects provide for a method of silencing: (i) an insect immuneresponse gene and/or (ii) an insect gene encoding for structuralcomponents of the insect midgut. In certain aspects, the methodcomprises providing for ingesting an isolated dsRNA molecule, an siRNAmolecule, an insecticidal composition, a host cell, a transgenic and/ortransplastomic plant, transplastomic plant and/or a seed, part, tissue,cell, or organelle as disclosed anywhere herein, to an insect.

Certain aspects provide for a method of protecting a plant from aninsect pest of the plant. In certain aspects, the method comprisestopically applying to a plant an isolated dsRNA molecule, an siRNAmolecule, and/or an insecticidal composition disclosed anywhere herein,and providing the plant in the diet of the insect pest. In certainaspects, the dsRNA is topically applied by expressing the dsRNA in amicrobe and topically applying the microbe onto the plant.

Certain aspects provide for a method of producing a plant resistance toa pest insect of said plant. In certain aspects, the method comprisestransforming a plant with a polynucleotide encoding the dsRNA moleculeand/or a construct or a dsRNA encoding segment thereof as disclosedanywhere herein, wherein the plant expresses a dsRNA molecule and/orproduces an siRNA disclose anywhere herein.

Certain aspects provide for a method of improving crop yield. In certainaspects, the method comprises growing a population of crop plantstransformed with a polynucleotide encoding a dsRNA molecule and/or aconstruct or a dsRNA encoding segment thereof wherein the plantexpresses a dsRNA molecule and/or produces an siRNA molecule asdiscloses anywhere herein. In certain aspects, the population oftransformed plants produces higher yields in the presence of pest insectinfestation than a control population of untransformed plants.

Certain aspects provide for a method for producing a plant resistantagainst a pest insect of said plant. In certain aspects, the methodcomprises: a) transforming a plant cell and/or organelle with apolynucleotide encoding a dsRNA molecule and/or a construct or a dsRNAencoding segment thereof as disclosed anywhere herein; b) regenerating aplant from the transformed plant cell and/or organelle; and c) growingthe transformed plant under conditions suitable for the expression ofsaid double stranded RNA molecule, wherein said transformed plant of (c)is resistant to the plant pest insect compared to an untransformedplant.

In certain of any of the aforementioned aspects, the method the dsRNA isingested by an actively feeding stage of the insect. In certain aspects,the ingestion of the dsRNA induces a melanotic response in the insectlarvae. In certain aspects, the ingestion of the dsRNA results inperturbation of gut microbial homeostasis. In certain aspects, theingestion of the dsRNA results in defective clearance of opportunisticmicrobes. In certain aspects, the ingestion of the dsRNA results indefective containment of gut microbes.

In certain of any of the aforementioned aspects, the silencing of thetarget gene results in reduced appetite and/or developmental defectsresulting in incomplete development and/or mortality and/or decrease thereproductive success of the insect. In certain aspects, the reducedappetite and/or developmental defects and/or mortality and/or reducedreproductive fitness of the insect is observed after sustained feedingfor at least 72 hours.

In certain of any of the aforementioned aspects, the insect is of theorder Lepidoptera, Coleoptera, Hemiptera, Blattodea, or Diptera. Incertain aspects, the insect is Manduca sexta (M. sexta) (tobaccohornworm), Spodoptera frugiperda (fall armyworm), Ostrinia nubilalis(European corn borer), Plutella xylostella (Diamondback moth),Leptinotarsa decemlineata Say (Colorado potato beetle), Diabrotica spp.(Corn rootworm complex), Tribolium castaneum (Red flour beetle),Popillia japonica (Japanese beetle), Agrilus planipennis (Emerald ashborer), Diaphorina citri (Asian citrus psyllid), Cimex lectularius (Bedbug), a cockroach or termite, or insect pests such as mosquitoes andflies.

In certain of any of the aforementioned aspects, the plant host isselected from the group consisting of Zea mays L (corn), Sorghum bicolor(sorghum), Setaria italica (fox tail millet), Pennisetum glaucum (Pearlmillet), Solanum tuberosum (potato), Oryza sativa (rice), Lycopersiconesculentum (tomato), Solanum melongena (eggplant), cultivars of theBrassica oleracea family, Citrus sinensis (Orange), trees of theOleaceae family, and crops of Rosaceae.

BRIEF DESCRIPTION OF THE DRAWINGS

The application file contains at least one photograph executed in color.Copies of this patent application publication with color photographswill be provided by the Office upon request and payment of the necessaryfee.

FIG. 1 shows schematic representation of bacterially ingestible dsRNAassay. (Kamath R S, et al. (2000) Genome Biol. 2: 1-10; Newmark et al.(2003) Proc. Natl. Acad. Sci. USA 100: 11861-11865).

FIG. 2A-C shows representative phenotypes of TH larvae. TH larvaeexposed to bacterially (HT115 (DE3)) expressed dsRNA against MIGGS RNAitargets MsPGRP2 (A); MsVMP1 (B); and negative control dsRNA againstCassava plant specific gene MeCAT1 (C).

FIG. 3A,B shows feeding activity of TH larvae exposed to dsRNA againstnegative control MeCAT1 (A) and MIGGS RNAi target MsPGRP2 (B) containingbacterial (HT115 (DE3)) plates.

FIG. 4 shows survival rates of healthy conventionally-reared (CR) andgerm-free (GF) first instar TH larvae exposed to bacterially (HT115(DE3)) expressed dsRNA against MIGGS RNAi targets MsPGRP2; MsVMP1 andnegative control dsRNA against Cassava plant specific gene MeCAT1. Thedifferences observed using 4 replicates/treatment were statisticallysignificant across all time points at a P value between P<0.001 toP<0.05.

FIG. 5 shows percentage melanotic reaction of healthyconventionally-reared (CR) and germ-free (GF) first instar TH larvaeexposed to bacterially (HT115 (DE3)) expressed dsRNA against MIGGS RNAitargets MsPGRP2; MsVMP1 and negative control dsRNA against Cassava plantspecific gene MeCAT1. The differences observed using 4replicates/treatment were statistically significant across all timepoints at a P value between P≤0.001 to P≤0.05.

FIG. 6 shows schematic representation of dsRNA producing L4440 vectorcontaining coding sequence of Cassava CAT1 (MeCAT1) and TH MIGGS RNAitargets MsPGRP2 and MsVMP1.

FIG. 7 shows midgut-preferred expression of two TH MIGGS RNAi targetgenes MsHEM and MsSPH3 in comparison to the control gene RPS3. Thecontrol cDNA libraries were derived from conventionally reared larvaeand treatment cDNA libraries were derived from TH larvae injected with75 CFU of E. coli. The control and treatment larvae were used to isolatehemolymph fraction (HL), dissect midgut (MDG) to obtain rest of the body(RB). The HL, MDG and RB were used for RNA isolation and cDNA synthesis.

FIG. 8 shows schematic representation of oral induction procedure forMIGGS RNAi target genes. The insect larvae were reared on inductionmedia containing live gram-negative bacteria E. coli and lyophilizedcell wall signatures from gram-positive bacteria and fungi, following apreviously published protocol (Wang et al. (2006). J. Biol. Chem.281(14): 9271-9278).

FIG. 9A,B shows expression of MIGGS RNAi target genes in TH larvae inresponse to feeding on induction media. Genes with immunity function(A,B) are induced (I) between 24-48 hours post larval exposure toinduction media and mostly not detected in the absence of induction(UI).

FIG. 9C,D also shows expression of MIGGS RNAi target genes in TH larvaein response to feeding on induction media. Genes with immunity function(C) are induced (I) between 24-48 hours post larval exposure toinduction media and mostly not detected in the absence of induction(UI). While, the genes essential for midgut structural integrity (D) areexpressed under both conditions.

FIG. 10A-F shows representative phenotypes of TH larvae (initial sizeshown in A) exposed to bacterially expressed dsRNA against insecticidalMIGGS-RNAi target genes MsToll2, MsSuc1, and MsβTub in comparison tonegative (B) and positive (C) control treatment. The insecticidalactivity is manifested as stunted growth and development, loss ofappetite and melanotic reaction (D-F) in comparison to regular growthand development observed with negative control treatment (B).

FIG. 11 shows percentage mortality of TH larvae feeding on bacteriallyexpressed dsRNA against insecticidal MIGGS RNAi targets MsToll2, MsSuc1,and MsβTub. The insecticidal MIGGS candidates confer statisticallysignificant mortality that is comparable to positive control treatment(MsVATPaseE). Data is average of 3-replicates/treatment ±SEM atp≤0.001(***).

FIG. 12A-C shows the induction of MIGGS-IRTGS in DBM larvae feeding oninduction media. The genes with immunity function (A-B) are induced (I)between 24-48 hours post larval exposure to induction media and mostlynot detected in the absence of induction (UI). While, the genesessential for midgut structural integrity (C) are expressed under bothconditions.

FIG. 13A-H shows representative phenotypes of Bt resistant DBM larvae(initial size shown in A) exposed to bacterially expressed dsRNA againstinsecticidal DBM MIGGS RNAi targets PxPGRP2 (D), PxIMD (E), PxβGRP2 (F),PxCAC (G), and PxCHS1 (H) in comparison to positive (C) and negativecontrol (B) treatment. The insecticidal activity is manifested asstunted growth and development, loss of appetite and melanotic reaction(D-H) in comparison to regular growth and development observed withnegative control treatment (B).

FIG. 14 shows percentage mortality of DBM larvae feeding on bacteriallyexpressed dsRNA against insecticidal MIGGS RNAi targets PxPGRP2, PxIMD,PxβGRP2, PxCAC, and PxCHS1. The insecticidal MIGGS candidates conferstatistically significant mortality that is comparable to positivecontrol treatment (MsVATPaseE). Data is average of3-replicates/treatment ±SEM at p≤0.001(***) p≤0.01(**).

FIG. 15A,B illustrates a sprayable RNAi set up using dsRNA against THMIGGS targets MsPGRP2, Ms βGRP2, MsCHS2, and MsVMP1. One cm² leaf discsfrom field soil grown tobacco plants (A) were drop inoculated withvarious concentrations of purified dsRNA against TH MIGGS RNAi targets(B). The bioassays were carried out for 5 days with 3 first instarlarvae per well and leaf discs changed at the end of every 24 hours.

FIG. 16 shows percentage mortality of TH larvae feeding on variousconcentrations of dsRNA against the core set of TH MIGGS RNAi targetsMsPGPRP2, MsβGRP2, MsCHS2, and MsVMP1. The leaf disc coated dsRNA causessignificant mortality at 8 and 16 μg of dsRNA concentration. The leafdisc coated dsRNA against three core MIGGS targets confers statisticallysignificant mortality that is comparable to positive control treatment(MsVATPaseE). Data is average of 3-replicates/treatment ±SEM atp≤0.001(***); p≤0.01(**) and p≤0.05(***).

FIG. 17A-H shows representative phenotypes of TH larvae (initial sizeshown in A and E) exposed to 8 and 16 μg of dsRNA against the core setof of MIGGS RNAi targets MsPGPRP2 (D), MsβGRP2 (F), MsCHS2 (G), andMsVMP1 (H). The insecticidal activity is comparable to the positivecontrol treatment (C) and manifested as stunted growth and development,loss of appetite and melanotic reaction (D,F-G) in contrast to theregular growth and development observed with negative control treatment(B).

FIG. 18A-D shows representative phenotypes of TH larvae feeding onleaves from transplastomic plants expressing dsRNA against core MIGGStargets MsPGRP2 (PTS-28-10 and PTS-28-7-B), MsβGRP2 (PTS-26-19 andPTS-26-4-C), and dsCHS2 (PTS-27-3-1 and PTS-27-13-1-D). The TH larvaefeeding on leaves expressing dsRNA against MIGGS targets MsPGRP2 (B),MsβGRP (C), and MsCHS2 (D) display stunted growth, development, loss ofappetite and melanotic reaction in comparison to negative controlPTS-27-13-2 (A).

FIG. 18E shows that the insecticidal activity of transplastomic lines ismanifested as significant reduction in mean weights in comparison tonegative control.

FIG. 18F shows that the transplastomic events confer significantmortality in comparison to negative control. The mortality rate wasscored on a 0-3 score were 0, 1, 2 and 3 indicated ≤0, 25, 50 or ≥50mortality respectively. Data is average of 6 replicates/treatment (N=24)±SEM at p≤0.001(***); p≤0.01(**) and p≤0.05(*).

FIG. 19 shows percentage mortality of Bt resistant DBM larvae feeding onvarious concentrations of dsRNA against the DBM orthologs of TH coreMIGGS RNAi targets PxPGPRP2, PxβGRP2, PxβTUB, and PxCHS1. The leaf disccoated dsRNA causes significant mortality at 0.5-1 μg of dsRNAconcentration. The leaf disc coated dsRNA against all core MIGGS targetsconfers statistically significant mortality between 0.5 μg and 1 μgdsRNA dosage that is comparable to positive control treatment(MsVATPaseE). Data is average of 3-replicates/treatment ±SEM atp≤0.01(**) and p≤0.05(*).

FIG. 20A-H shows representative phenotypes of Bt resistant DBM larvae(initial size shown in A and E) exposed to 0.5-1 μg of dsRNA against theDBM orthologs of TH core MIGGS RNAi targets PxPGPRP2(D), PxβGRP2(F),PxβTUB(G), and PxCHS1(H). The insecticidal activity is comparable to thepositive control treatment (C) and manifested as stunted growth anddevelopment, loss of appetite and melanotic reaction (D,F-H) in contrastto the regular growth and development observed with negative controltreatment (B).

FIG. 21A-D illustrates MIGGS-IRTG induction procedure in FAW feeding onwheat plants grown microbe rich field soil (A) and microbe depletedsterile surface (B). Ten first instar FAW larvae (C) were infested intoeach pot containing five wheat seedlings and contained using porousnetting material (D). The larval samples were collected at various timepoints after infestation and used for pooled RNA-Seq approach toidentify differential expressed transcripts in response to induction bythe microbes in the filed soil.

FIG. 22A,B shows bi-clustering comparison of differential geneexpression in FAW larvae feeding on microbe-depleted plants (A) incomparison to larvae feeding on microbe rich plants (B). RNA-Seq dataindicated that plants growing on field soil caused preferentialup-regulation of MIGGS pathway genes in FAW. In total, 100 differentialexpressed genes were identified, 30 of which were associated with MIGGSpathways. Notably, FAW orthologs of TH insecticidal targets includingPGRP2, βGRP2 and IMD were captured in the data set, indicating clearlythat MIGGS pathway genes are up regulated in response to insect feedingon plants exposed to soil microbiome.

FIG. 23A-F shows representative phenotypes of FAW larvae exposed to puredsRNA against FAW orthologs of TH core MIGGS RNAi targets SFCHS2 (B),SFβGRP2 (C), SFβGRP2 (D), and two novel MIGGS RNAi targets from RNA-Seqstudy SFRC (un-annotated-E) and C-type lectin-6 (SFCTL-F). Leaf discscoated with dsRNA causes reduced growth, development and loss ofappetite in comparison to negative control treatment (A) when suppliedat 8 and 16 μg of dsRNA concentration per leaf disc.

FIG. 23G,H illustrates from FIG. 23A-F significant weight reduction (G)and mortality (H). The rates of mortality was scored on a 0-3 scalewhere 0, 1, 2 and 3 indicated ≤0, 25, 50 or ≥50 mortality respectively.The dsRNA treatments imposed caused statistically significant reductionin mean weights (G) that translated into significant rates of mortality(H) in comparison to negative control. Data is average of 3replicates/treatment ±SEM at p≤0.001(***); p≤0.01(**) and p≤0.05(*).

FIG. 24 shows mean weights (A) of FAW larvae exposed to 16 μg of puredsRNA against newly discovered MIGGS RNAi targets from the RNA-Seq data.The rates of mortality was scored on a 0-3 score were 0, 1, 2 and 3indicated ≤0, 25, 50 or ≥50 mortality respectively. The dsRNA treatmentsimposed caused statistically significant reduction in mean weights (A)that also translated into significant rates of mortality (B) incomparison to negative control. Data is average of 3replicates/treatment ±SEM at p≤0.001(***); p≤0.01(**) and p≤0.05(*).

FIG. 25 shows representative phenotypes of RFB beetles feeding on riceflour mixed with 1 μg of pure dsRNA against core MIGGS targets in RFBTcPGRP2, MsβGRP2, dsCHS2, and a previously discovered target MDGP. TheRFB beetles feeding on dsRNA against all MIGGS targets displayedsignificant mortalities at the end of 72 hours of feeding in comparisonto negative control treatment (TE). The rates of mortality weresignificantly higher than negative control treatment and were comparableto the positive control treatment TcvATPaseE. Mortality rate was scoredon a 0-3 scale were 0, 1, 2 and 3 indicated ≤0, 25, 50 or ≥50 mortalityrespectively. Data is average of 6 replicates/treatment (N=18) ±SEM atp≤0.001(***); p≤0.01(**) and p≤0.05(*).

FIG. 26A,B shows imageJ analyzed RT-PCR data correlating TH larvalphenotypes observed when exposed to 8-16 μg pure dsRNA against apositive control (A) and a core insecticidal MIGGS RNAi target MsβGRP2(B). The transcript down regulation is correlated with the larvalphenotypes observed in FIG. 17. For each pair of vertical bars, 8 μgpure dsRNA is the left bar and 16 μg pure dsRNA is the right bar.

FIG. 26C,D shows imageJ analyzed RT-PCR data correlating TH larvalphenotypes observed when exposed to 8-16 μg pure dsRNA against core setof insecticidal MIGGS RNAi targets MsPGPRP2 (C) and MsCHS2 (D). Thetranscript down regulation is correlated with the larval phenotypesobserved in FIG. 17. For each pair of vertical bars, 8 μg pure dsRNA isthe left bar and 16 μg pure dsRNA is the right bar.

DETAILED DESCRIPTION Overview

RNAi-mediated gene-silencing offers a sustainable alternative approachto insect control. Most of the successful RNAi-based pest controlstrategies thus far employ homology dependent silencing of essentialgene functions. Despite this, effective RNAi-based crop protection islacking for Lepidopteran pests, due to their variable sensitivity toingested double stranded RNA (dsRNA). (Terenius O, et al. (2011). J.Insect Physiol. 57(2): 231-245).

Plant pests are in constant contact with, and ingest significant amountof microbes during herbivory. (Gayatri Priya N. et al. (2012). 7(1),PLos ONE. E30768; Peñuelas and Terradas (2014). 19(5): Trends Plant Sci.278-280; Engel and Moran (2013). FEMS microbiol. rev. 37(5): 699-735).This interaction between ingested microbes and insect midgut is oftenconsidered passive. Recent studies suggest, however, an active role ofmidgut specific immune responses in reducing variation of core microbialcommunities during insect herbivory through the activation of patternrecognition receptors (PRR). (Casanova-Torres and Goodrich-Blair (2013).Insects. 4: 320-338; Tang X, et al. (2012) 7(7) PLoS ONE:e36978; Ryu JH. et al. (2008). Science. 37(5): 777-82; Shrestha S. et al. (2009). J.Asia Pac. Entomol. 12: 277-283; Buchon, N. et al. (2013). Front. Cell.Infect. Microbiol. 11: 615-626). Further, maintenance of core gutmicrobial communities via active immune responses and/or theircontainment in the midgut is key to successful herbivory.

Although, the core innate immune response pathways are conserved, theirspecific components are under strong selection for diversification.(Casanova-Torres and Goodrich-Blair (2013). Insects. 4: 320-338).Therefore, it is contemplated herein that these pathways provide noveland specific targets for devising sustainable pesticidal RNAibiotechnologies against insect pests. Although gut immune responses havebeen studied from an immunological perspective, their activemanipulation via genetic engineering for pest protection is currentlylacking.

Provided herein is the identification of “insect RNAi target genes”(IRTGs) involved in gut microbial clearance and/or containment inducedby microbes ingested during feeding and/or active feeding (referred toherein as microbe-induced gut specific genes (MIGGS)) and examples of anovel biotechnology for insect protection via inter-specific silencingof MIGGS-IRTGs. In certain aspects, the MIGGS-IRTGs areLepidoptera-specific. For example, in certain aspects detailed below,the insect is Manduca sexta (M. sexta; Lepidoptera) (tobacco hornworm(TH)). For example, in certain aspects detailed below, the insect isSpodoptera frugiperda (fall armyworm (FAW)). For example, in certainaspects detailed below, the insect is Plutella xylostella (Diamondbackmoth (DBM). For example, in certain aspects detailed below, the insectis Ostrinia nubilalis (European corn borer). Still, it is alsoconsidered understood that successful, feeding-induced loss of appetite,developmental defects, and/or lethality has the potential to provideprotection beyond the order Lepidoptera in an orthologous manner. Forexample, protection against coleopteran pests such as Leptinotarsadecemlineata (Say) (Colorado potato beetle), Diabrotica spp. (Cornrootworm complex), and Tribolium castaneum (Red Flour Beetle (RFB)).Additionally, this MIGGS-RNAi technique may allow containment of diseasetransmitting insect vectors and/or enable further manipulation of theplant-microbe-insect interactions for devising pesticidal RNAi for cropprotection.

In certain aspects detailed below, silencing of a target gene can resultin reduced appetite and/or developmental defects and/or mortality and/orreduced fitness of the insect. In certain aspects these effects areobserved after sustained feeding for at least about 24, 36, 48, or 72hours, or any time inbetween.

Definitions

To the extent necessary to provide descriptive support, the subjectmatter and/or text of the appended claims is incorporated herein byreference in their entirety.

It will be understood by all readers of this written description thatthe exemplary embodiments described and claimed herein may be suitablypracticed in the absence of any recited feature, element or step thatis, or is not, specifically disclosed herein.

It is to be noted that the term “a” or “an” entity refers to one or moreof that entity; for example, “a dsRNA molecule,” is understood torepresent one or more dsRNA molecules. As such, the terms “a” (or “an”),“one or more,” and “at least one” can be used interchangeably herein.

Furthermore, “and/or” where used herein is to be taken as specificdisclosure of each of the specified features or components with orwithout the other. Thus, the term and/or” as used in a phrase such as “Aand/or B” herein is intended to include “A and B,” “A or B,” “A”(alone), and “B” (alone). Likewise, the term “and/or” as used in aphrase such as “A, B, and/or C” is intended to encompass each of thefollowing embodiments: A, B, and C; A, B, or C; A or C; A or B; B or C;A and C; A and B; B and C; A (alone); B (alone); and C (alone).

It is understood that wherever aspects are described herein with thelanguage “comprising,” otherwise analogous aspects described in terms of“consisting of” and/or “consisting essentially of” are also provided.

Unless defined otherwise, technical and scientific terms used hereinhave the same meaning as commonly understood by one of ordinary skill inthe art to which this disclosure is related. Numeric ranges areinclusive of the numbers defining the range. Even when not explicitlyidentified by “and any range in between,” or the like, where a list ofvalues is recited, e.g., 1, 2, 3, or 4, unless otherwise stated, thedisclosure specifically includes any range in between the values, e.g.,1 to 3, 1 to 4, 2 to 4, etc.

The headings provided herein are solely for ease of reference and arenot limitations of the various aspects or aspects of the disclosure,which can be had by reference to the specification as a whole.

As used herein, the term “non-naturally occurring” condition, substance,polypeptide, polynucleotide, composition, entity, plant, organism,individual, and/or any combination thereof, or any grammatical variantsthereof and the like, is a conditional term that explicitly excludes,but only excludes, those forms that are well-understood by persons ofordinary skill in the art as being “naturally-occurring,” or that are,or might be at any time, determined or interpreted by a judge or anadministrative or judicial body to be, “naturally-occurring.”

As used herein, the term “identity,” e.g., “percent identity” to anamino acid sequence or to a nucleotide sequence disclosed herein refersto a relationship between two or more amino acid sequences or betweentwo or more nucleotide sequences. When a position in one sequence isoccupied by the same nucleic acid base or amino acid in thecorresponding position of the comparator sequence, the sequences aresaid to be “identical” at that position. The percentage of “sequenceidentity” is calculated by determining the number of positions at whichthe identical nucleic acid base or amino acid occurs in both sequencesto yield the number of “identical” positions. The number of “identical”positions is then divided by the total number of positions in thecomparison window and multiplied by 100 to yield the percentage of“sequence identity.” Percentage of “sequence identity” is determined bycomparing two optimally aligned sequences over a comparison window. Inorder to optimally align sequences for comparison, the portion of anucleotide or amino acid sequence in the comparison window can compriseadditions or deletions termed gaps while the reference sequence is keptconstant. An optimal alignment is that alignment which, even with gaps,produces the greatest possible number of “identical” positions betweenthe reference and comparator sequences. Percentage “sequence identity”between two sequences can be determined using, e.g., the program “BLAST”which is available from the National Center for BiotechnologyInformation, and which program incorporates the programs BLASTN (fornucleotide sequence comparison) and BLASTP (for amino acid sequencecomparison), which programs are based on the algorithm of Karlin andAltschul ((1993). Proc. Natl. Acad. Sci. USA. 90(12): 5873-5877).

As used herein, the term “complementary” refers to the ability ofpolynucleotides to form base pairs with one another. Base pairs aretypically formed by hydrogen bonds between nucleotide units inantiparallel polynucleotide strands. Complementary polynucleotidestrands can base pair in the Watson-Crick manner (e.g., A to T, A to U,C to G), or in any other manner that allows for the formation ofduplexes. When using RNA as opposed to DNA, uracil (U) rather thanthymine (T) is the base that is considered to be complementary toadenosine. However; when a U is denoted in the context of the presentinvention, the ability to substitute a T is implied, unless otherwisestated.

As used herein, the term “polypeptide” is intended to encompass asingular “polypeptide” as well as plural “polypeptides,” and refers to amolecule composed of monomers (amino acids) linearly linked by peptidebonds (also known as amide bonds). The term “polypeptide” refers to anychain or chains of two or more amino acids, and does not refer to aspecific length of the product. Thus, peptides, dipeptides, tripeptides,oligopeptides, “protein,” “amino acid chain,” or any other term used torefer to a chain or chains of two or more amino acids are includedwithin the definition of “polypeptide,” and unless specifically statedotherwise the term “polypeptide” can be used instead of, orinterchangeably with any of these terms. The term “polypeptide” is alsointended to refer to the products of post-expression modifications ofthe polypeptide, including without limitation glycosylation,acetylation, phosphorylation, amidation, derivatization by knownprotecting/blocking groups, proteolytic cleavage, or modification bynon-standard amino acids. A polypeptide can be derived from a naturalbiological source or produced by recombinant technology, but is notnecessarily translated from a designated nucleic acid sequence. Thus, itcan be generated in any manner, including by chemical synthesis.

As used herein, the term “protein” refers to a single polypeptide, i.e.,a single amino acid chain as defined above, but can also refer to two ormore polypeptides that are associated, e.g., by disulfide bonds,hydrogen bonds, or hydrophobic interactions, to produce a multimericprotein.

As used herein, the term “nucleotide” refers to a ribonucleotide or adeoxyribonucleotide or modified form thereof, as well as an analogthereof. Nucleotides include species that comprise purines, e.g.,adenine, hypoxanthine, guanine, and their derivatives and analogs, aswell as pyrimidines, e.g., cytosine, uracil, thymine, and theirderivatives and analogs. Further, the term nucleotide also includesthose species that have a detectable label, such as for example aradioactive or fluorescent moiety, or mass label attached to thenucleotide.

As used herein, the term “polynucleotide” refers to polymers ofnucleotides, and includes but is not limited to DNA, RNA, DNA/RNAhybrids including polynucleotide chains of regularly and/or irregularlyalternating deoxyribosyl moieties and ribosyl moieties (i.e., whereinalternate nucleotide units have an —OH, then and —H, then an —OH, thenan —H, and so on at the 2′ position of a sugar moiety), andmodifications of these kinds of polynucleotides, wherein the attachmentof various entities or moieties to the nucleotide units at any positionare included. The term “polynucleotide” is also intended to encompass asingular nucleic acid as well as plural nucleic acids, and refers to anisolated nucleic acid molecule or construct, e.g., messenger RNA (mRNA)or plasmid DNA (pDNA). A polynucleotide can comprise a conventionalphosphodiester bond or a non-conventional bond (e.g., an amide bond,such as found in peptide nucleic acids (PNA)). A polynucleotide can besingle stranded or double stranded.

As used herein, the term “nucleic acid” refers to any one or morenucleic acid segments, e.g., DNA or RNA fragments, present in apolynucleotide. By “isolated” nucleic acid or polynucleotide is intendeda nucleic acid molecule, DNA or RNA, which has been removed from itsnative environment. For example, a recombinant polynucleotide encoding apolypeptide subunit contained in a vector is considered isolated asdisclosed herein. Further examples of an isolated polynucleotide includerecombinant polynucleotides maintained in heterologous host cells orpurified (partially or substantially) polynucleotides in solution.Isolated RNA molecules include in vivo or in vitro RNA transcripts ofpolynucleotides. Isolated polynucleotides or nucleic acids furtherinclude such molecules produced synthetically. In addition,polynucleotide or a nucleic acid can be or can include a regulatoryelement such as a promoter, ribosome binding site, or a transcriptionterminator.

As used herein, a “coding region” is a portion of nucleic acidcomprising codons translatable into amino acids. Although a “stop codon”(TAG, TGA, or TAA) is not translated into an amino acid, it can beconsidered to be part of a coding region, but any flanking sequences,for example 5′ untranslated regions (5′ UTRs; also known as a leadersequence), 3′ untranslated regions (3′ UTRs; also known as a trailersequence), promoters, ribosome binding sites, transcriptionalterminators, introns, and the like, are not part of a coding region. Twoor more coding regions can be present in a single polynucleotideconstruct, e.g., on a single vector, or in separate polynucleotideconstructs, e.g., on separate (different) vectors. Furthermore, anyvector can contain a single coding region, or can comprise two or morecoding regions, e.g., a single vector can separately encode a selectionmarker gene and a gene of interest. In addition, a vector,polynucleotide, or nucleic acid can encode heterologous coding regions,either fused or unfused to a nucleic acid encoding a polypeptide subunitor fusion protein as provided herein. Heterologous coding regionsinclude without limitation specialized elements or motifs, such as asecretory signal peptide or a heterologous functional domain.

A variety of transcription regulatory regions are known to those skilledin the art. These include, without limitation, transcription regulatoryregions that function in vertebrate cells, such as, but not limited to,promoter and enhancer segments from cytomegaloviruses (the immediateearly promoter, in conjunction with intron-A), simian virus 40 (theearly promoter), and retroviruses (such as Rous sarcoma virus). Othertranscription regulatory regions include those derived from vertebrategenes such as actin, heat shock protein, bovine growth hormone andrabbit β-globin, as well as other sequences capable of controlling geneexpression in eukaryotic cells. Additional suitable transcriptionregulatory regions include tissue-specific promoters and enhancers.

Similarly, a variety of translation regulatory elements are known tothose of ordinary skill in the art. These include, but are not limitedto ribosome binding sites, translation initiation and terminationcodons, and elements derived from picornaviruses (particularly aninternal ribosome entry site, or IRES).

As used herein, the term “vector” is nucleic acid molecule as introducedinto a host cell or organelle, thereby producing a transformed host cellor organelle. A vector can include nucleic acid sequences that permit itto replicate in a host cell, such as an origin of replication. A vectorcan also include one or more selectable marker gene and other geneticelements known in the art. Illustrative types of vectors includeplasmids, phages, viruses and retroviruses.

As used herein, the term “transformed” cell or organelle, or a “host”cell organelle, is a cell or organelle into which a nucleic acidmolecule has been introduced by molecular biology techniques. As usedherein, the term transformation encompasses those techniques by which anucleic acid molecule can be introduced into such a cell or organelle,including transfection with viral vectors, transformation with plasmidvectors, and introduction of naked DNA by electroporation, lipofection,and particle gun acceleration. A transformed cell or a host cell can bea bacterial cell or a eukaryotic cell.

As used herein, the term “expression” refers to a process by which agene produces a biochemical, for example, a polynucleotide or apolypeptide. The process includes any manifestation of the functionalpresence of the gene within the cell including, without limitation, geneknockdown as well as both transient expression and stable expression. Itincludes without limitation transcription of the gene into messenger RNA(mRNA), and the translation of such mRNA into polypeptide(s). It alsoincludes without limitation transcription of the gene into an RNAmolecule that is not translated into a polypeptide but is capable ofbeing processed by cellular RNAi mechanisms. If the final desiredproduct is a biochemical, expression includes the creation of thatbiochemical and any precursors. Expression of a gene produces a “geneproduct.” As used herein, a gene product can be either a nucleic acid,e.g., an RNA produced by transcription of a gene or a polypeptide thatis translated from a mRNA transcript. Gene products described hereinfurther include nucleic acids with post transcriptional modifications,e.g., polyadenylation, or polypeptides with post translationalmodifications, e.g., methylation, glycosylation, the addition of lipids,association with other protein subunits, proteolytic cleavage, and thelike.

As used herein the term “engineered” includes manipulation of nucleicacid or polypeptide molecules by synthetic means (e.g. by recombinanttechniques, in vitro peptide synthesis, by enzymatic or chemicalcoupling of peptides or some combination of these techniques).

As used herein, the term “hpRNA” refers to hairpin RNA comprising asingle-stranded loop region and a base-paired stem of an inverselyrepeated sequence. hpRNA can be generated from an hpRNA construct (orvector) and/or an hpRNA transgene comprising an inversely-repeatedsequence of the RNAi target gene with a spacer region between therepeats. The RNA transcribed from such a sequence self-hybridizes toform a hairpin structure. The stem can be used as a substrate for thegeneration of siRNAs, but few or none are generated from the loop. Sincea spacer region is needed for the stability of the transgene construct,but is not involved in siRNA production, an intron sequence is oftenused in this position. (Watson J M, et al. (2005). FEBS Letters. 579:5982-8987).

As used herein, the term “siRNA” refers to small (or short) interferingRNA (or alternatively, silencing RNA) duplexes that are capable ofinducing the RNA interference (RNAi) pathway. These molecules can varyin length (generally between 18-30 base pairs) and contain varyingdegrees of complementarity to their target mRNA in the antisense strand.Some, but not all, siRNA have unpaired overhanging bases on the 5′ or 3′end of the sense strand and/or the antisense strand. The term “siRNA”includes duplexes of two separate strands, as well as single strandsthat can form hairpin structures comprising a duplex region.

As used herein, the phrase “duplex region” refers to the region in twocomplementary or substantially complementary polynucleotides that formbase pairs with one another, either by Watson-Crick base pairing or anyother manner that allows for a stabilized duplex between polynucleotidestrands that are complementary or substantially complementary. Forexample, a polynucleotide strand having 21 nucleotide units can basepair with another polynucleotide of 21 nucleotide units, yet only 19bases on each strand are complementary or substantially complementary,such that the “duplex region” has 19 base pairs. The remaining basesmay, for example, exist as 5′ and 3′ overhangs. Further, within theduplex region, 100% complementarity is not required; substantialcomplementarity is allowable within a duplex region. Substantialcomplementarity as used herein refers to 79% or greater complementarity.For example, a mismatch in a duplex region consisting of 19 base pairsresults in 94.7% complementarity, rendering the duplex regionsubstantially complementary.

As used herein, the phrase “gene silencing” refers to a process by whichthe expression of a specific gene product is lessened or attenuated.Silencing of a gene does not require that the expression or presence ofthe gene product is completely absent, but that in the context (e.g.,comparing expression of a target gene in a plant expressing a genesilencing nucleic acid compared to a control plant or the health of aninsect feeding on a gene silencing nucleic acid compared to a controlinsect), an observable effect in comparison to a control is observed.While gene silencing can take place by a variety of pathways, unlessspecified otherwise, as used herein, gene silencing refers to decreasesin gene product expression that results from RNA interference (RNAi) asunderstood by one of ordinary skill in the art. The level of genesilencing can be measured by a variety of means, including, but notlimited to, measurement of transcript levels by Reverse transcriptionpolymerase chain reaction (PCR), Northern Blot Analysis, B-DNAtechniques, transcription-sensitive reporter constructs, expressionprofiling (e.g. DNA chips), and related technologies. Alternatively, thelevel of silencing can be measured by assessing the level of the proteinencoded by a specific gene. This can be accomplished by performing anumber of studies including Western Analysis, measuring the levels ofexpression of a reporter protein that has e.g. fluorescent properties(e.g. GFP) or enzymatic activity (e.g. alkaline phosphatases), orseveral other well-known procedures. Further, gene silencing can beassessed by its effect on a pest insect such as resulting in reducedappetite and/or developmental defects and/or mortality of an insect.

As used herein, the term “control” is consistent with itswell-established scientific use that refers to a standard of comparisonrecognized by one of ordinary skill in the art as having arepresentative level of expression, phenotype, resistance, feeding,mortality, development, etc. Further, one of ordinary skill in the artwill recognize, for example, that a statistical outlier and/ornon-representative result produced by chance, abnormal environmentalcondition, manipulation, or other reason, that varies from a standardrepresentation, would not be an appropriate control.

As used herein, “microbe-induced gut specific genes (MIGGS)” refers to agene or group of genes expressed in the insect midgut in response tomicrobes ingested during normal process of insect feeding and primarilyfunctioning to clear or respond to the ingested microbes and/or containthe microbes to insect gut via maintenance of midgut structuralintegrity.

As used herein, “actively feeding stage of the insect” refers to allfeeding stages of insects with both complete and incompletemetamorphosis.

Nucleic Acids for Gene Silencing

Provided herein are nucleic acid molecules for use in, among otherthings, crop protection from insect pests. In certain aspects disclosedherein, the nucleic acid molecules are isolated. The nucleic acidmolecules specifically target certain insect genes (referred to hereininterchangeably as “target genes,” “RNAi target genes,” “insect RNAitarget genes,” and “IRTGs”), in insects for gene silencing. For example,in certain aspects, the nucleic acid molecules target certain insectmicrobe-induced gut gene (MIGGS) RNAi targets. In certain aspects, thesilencing of a target gene occurs when a nucleic acid molecule of thisdisclosure is ingested by an insect. In certain aspects, the target geneis an insect gene that is implicated in insect immune responses (type 1MIGGS RNAi target). A critical immune response gene is a geneticallytractable nuclear or cytoplasmic loci that is important for providingcellular and/or humoral defense in insects against internalmicroorganisms, external microorganisms, and/or other insect parasites.In certain aspects, the immune response genes (type 1 MIGGS RNAi target)can also be a pattern recognition receptor (PRR) gene (Casanova-Torresand Goodrich-Blair (2013). Insects. 4:320-338). A PPR gene is agenetically tractable loci of an insect that encodes soluble or membranebound proteins that recognize signatures associated with and/or releasedby microorganisms. PRR genes can activate or be activated by the immuneresponse pathways to minimize microbial infection and can beco-regulated by the immune deficiency (IMD) pathway (Tang X, et al.(2012) 7(7) PLoS ONE:e36978; Ryu J H. et al. (2008). Science. 37(5):777-82; Shrestha S. et al. (2009). In certain aspects the PRR type genesare co-regulated by the immune deficiency (IMD) pathway in TH wereidentified, these genes having been recently summarized.(Casanova-Torres and Goodrich-Blair (2013). Insects (4): 320-338; ZhongX, et al. (2012). Insect Biochem. Mol. Biol. 42(7): 514-524); Zhang X,et al. (2015). Insect Biochem. Mol. Biol. 62:38-50; Cao X, et al.(2015). Insect Biochem. Mol. Biol. 62:64-74; Kanost M R, et al. (2016).Insect Biochem. Mol. Biol 76:118-147). In certain aspects, the targetgene is an insect gene that is necessary for structural integrity ofinsect organs including the mid-gut and also facilitates the containmentof the ingested microbes to the insect gut. (type 2 MIGGS RNAi target).In certain aspects, the target gene is an insect midgut structuralcomponent gene (type 2) (Odman-Naresh et al. (2013). PLoS ONE 8:e82015.10.1371/journal.pone.0082015). A midgut structural component gene is agenetically tractable loci in an insect that encodes chitin fibrils,proteins, or glycoproteins that form a protective sac-like structurecalled peritrophic matrix enveloping the insect food bolus/midgut alsofunctioning to contain the ingested microbes in the gut (Engel and Moran(2013). FEMS Microbiol Rev. 37 699-735). In certain aspects, the targetgenes (type 1 and 2 MIGGS RNAi targets) are predominantly expressed inthe insect midgut, for example, abundantly and/or exclusively expressedin the larval and/or adult insect midgut in response to active feedingand/or microbial infection and/or responding to microbes ingested duringfeeding. In certain aspects, the target gene is induced predominantly ina midgut specific manner during active feeding (type 1 and type 2 MIGGSRNAi targets). The midgut abundance of both type 1 and 2 MIGGS RNAitarget genes may mitigate problems associated with reduced amounts ofbioavailability.

Representative examples of insect MIGGS RNAi target genes and theirnucleic acid sequences identified from published literature are providedherein. In certain aspects, the target gene is one or more of M.sexta-Hemolin (MsHEM), M. sexta-Serine proteinase homolog 3 (MsSPH-3),M. sexta-Peptidoglycan recognition protein 2 (MsPGRP2), M. sexta-Beta-1,3-glycan-recognition protein 2 (MsβGRP2), M. sexta-Relish family protein2A (MsREL2A), M. sexta-Dorsal (MsDor), M. sexta-Spätzle (MsSPZ1A), M.sexta-Toll receptor (MsTOLL), M. sexta-Scolexin A (MsSCA1), M.sexta-Hemolymph proteinase 18 (MsHP18), M. sexta-Transferrin (MsTRN), M.sexta-Arylphorin beta subunit (MsARP), M. sexta-Chymotrypsinogen-likeprotein 1 (MsCTL1), M. sexta-Valine Rich Midgut Protein (MsVMP1), M.sexta-Imd (MsImd), M. sexta-FADD (MsFADD), M. sexta-Dredd (MsDRD), M.sexta-Relish F (MsReIF), M. sexta-Cdc42 (MsCdc42), M. sexta-Dsor1(MsDsor1), M. sexta-Fos (MsFos), M. sexta-Jra (MsJra), M. sexta-Caudal(MsCAD1), M. sexta-Atg8 (MsAtg8), M. sexta-Atg13 (MsAtg13), M.sexta-IAP1 (MsIAP1), M. sexta-Chitin synthase 2 (MsChs2), M. sexta-Betafructofuranosidase 1 (MsSuc1), and orthologs thereof.

In certain aspects, the target gene is one or more of M. sexta-Hemolin(MsHEM), M. sexta-Serine proteinase homolog 3 (MsSPH-3), M.sexta-Peptidoglycan recognition protein 2 (MsPGRP2), M. sexta-Beta-1,3-glycan-recognition protein 2 (MsβGRP2), M. sexta-Relish family protein2A (MsREL2A), M. sexta-Dorsal (MsDor), M. sexta-Spätzle (MsSPZ1A), M.sexta-Toll receptor (MsTOLL), M. sexta-Scolexin A (MsSCA1), M.sexta-Hemolymph proteinase 18 (MsHP18), M. sexta-Transferrin (MsTRN), M.sexta-Arylphorin beta subunit (MsARP), M. sexta-Chymotrypsinogen-likeprotein 1 (MsCTL1), M. sexta-Valine Rich Midgut Protein (MsVMP1), M.sexta-Imd (MsImd), M. sexta-FADD (MsFADD), M. sexta-Dredd (MsDRD), M.sexta-Relish F (MsReIF), M. sexta-Cdc42 (MsCdc42), M. sexta-Dsor1(MsDsor1), M. sexta-Fos (MsFos), M. sexta-Jra (MsJra), M. sexta-Caudal(MsCAD1), M. sexta-Atg8 (MsAtg8), M. sexta-Atg13 (MsAtg13), M.sexta-IAP1 (MsIAP1), M. sexta-Chitin synthase 2 (MsChs2), and M.sexta-Beta fructofuranosidase 1 (MsSuc1), M. sexta-Sickie (MsSck), M.sexta-Akirin (MsAki), M. sexta-Cactus (MsCac), M. sexta-Gloverin (MsGlv)and M. sexta-Beta-1-tubulin (MsβTub).

In certain aspects, the target gene is an ortholog of one or more of M.sexta-Hemolin (MsHEM), M. sexta-Serine proteinase homolog 3 (MsSPH-3),M. sexta-Peptidoglycan recognition protein 2 (MsPGRP2), M. sexta-Beta-1,3-glycan-recognition protein 2 (MsβGRP2), M. sexta-Relish family protein2A (MsREL2A), M. sexta-Dorsal (MsDor), M. sexta-Spätzle (MsSPZ1A), M.sexta-Toll receptor (MsTOLL), M. sexta-Scolexin A (MsSCA1), M.sexta-Hemolymph proteinase 18 (MsHP18), M. sexta-Transferrin (MsTRN), M.sexta-Arylphorin beta subunit (MsARP), M. sexta-Chymotrypsinogen-likeprotein 1 (MsCTL1), M. sexta-Valine Rich Midgut Protein (MsVMP1), M.sexta-Imd (MsImd), M. sexta-FADD (MsFADD), M. sexta-Dredd (MsDRD), M.sexta-Relish F (MsReIF), M. sexta-Cdc42 (MsCdc42), M. sexta-Dsor1(MsDsor1), M. sexta-Fos (MsFos), M. sexta-Jra (MsJra), M. sexta-Caudal(MsCAD1), M. sexta-Atg8 (MsAtg8), M. sexta-Atg13 (MsAtg13), M.sexta-IAP1 (MsIAP1), M. sexta-Chitin synthase 2 (MsChs2), M. sexta-Betafructofuranosidase 1 (MsSuc1), and other IMD pathway or structuralintegrity genes.

One of ordinary skill in the art would understand that nucleic acidmolecules can be, for example, deoxyribonucleic acid (DNA) orribonucleic acid (RNA). In certain aspects of any target gene silencingnucleic acid molecule described anywhere herein, the nucleic acidmolecule is a DNA molecule. In certain aspects of any target genesilencing nucleic acid molecule described anywhere herein, the nucleicacid molecule is a RNA molecule. In certain aspects of any target genesilencing nucleic acid molecule described anywhere herein, the RNAmolecule is a double stranded molecule (dsRNA), for example, for use inthe RNA interference (RNAi) process. As used herein, a dsRNA molecule isa RNA molecule comprising at least one annealed, double stranded region.In certain aspects, the double stranded region comprises two separateRNA strands annealed together. In certain aspects, the double strandedregion comprises one RNA strand annealed to itself, for example, as canbe formed when a single RNA strand contains an inversely repeatedsequences with a spacer in between. One of ordinary skill in the artwill understand that complementary nucleic acid sequences are able toanneal to each other but that two sequences need not be 100%complementary to anneal. The amount of complementarity needed forannealing can be influenced by the annealing conditions such astemperature, pH, and ionic condition. In certain aspects, the annealedRNA sequences are 100% complementary across the annealed region. Incertain aspects, the annealed RNA sequences are less than 100%complementary across the annealed region but have enough complementarityto anneal within their environment, such as in a host cell or the gut ofan insect. In certain aspects, the annealed RNA sequences aresubstantial complementarity as defined elsewhere herein.

It is contemplated that the nucleic acid molecules disclosed anywhereherein for the silencing of target genes derive their specificity fromcomprising a nucleic acid sequence that is complementary orsubstantially complementary to at least a portion of a target genesequence. Substantially complementary sequences, however, may be morelikely to have reduced specificity and produce off-target effects. Asreferred to anywhere herein, a target gene sequence can include at leastthe target gene protein coding region, the 5′ untranslated region (5′UTR), and/or the 3′ untranslated region (3′ UTR) and any portion orcombination thereof. For example, predicted UTR regions can beidentified using previously established criteria (Siepel, et al. (2005).Genome Res. 15: 1034-1050) when corresponding genomic sequences areavailable.

In certain aspects, an isolated double stranded RNA (dsRNA) moleculecomprises a nucleic acid sequence complementary to about 21 to 2000contiguous nucleotides of a target gene sequence discloses anywhereherein. For example, in certain aspects, an isolated double stranded RNA(dsRNA) molecule comprises a nucleic acid sequence complementary toabout any of 21, 22, 23, 24, 25, 30, 40, 50, 60, 100, 120, 200, 240,300, 400, 500, 600, 650, 750, 1000 to about any of 23, 24, 25, 30, 40,50, 100, 200, 300, 400, 500, 600, 650, 750, 1000, or 2000 contiguousnucleotides of a target gene sequence. For example, in certain aspects,an isolated dsRNA molecule comprises a nucleic acid sequencecomplementary to about 100 to 1000 or about 200 to 1000 contiguousnucleotides of a target gene sequence. For example, in certain aspects,an isolated dsRNA molecule comprises a nucleic acid sequencecomplementary to about 100 to 1000 or about 200 to 1000 contiguousnucleotides of the protein coding region of a target gene sequence. Forexample, in certain aspects, an isolated dsRNA molecule comprises anucleic acid sequence complementary to about 100 to 1000 or about 200 to1000 contiguous nucleotides of the 5′ UTR region or the 3′ UTR region ofa target gene sequence. In certain aspects, the isolated dsRNA moleculecomprises a nucleic acid sequence complementary to a contiguous regioncomprising at least about 50%, 60%, 70%, 80%, 90%, 95%, 98%, 99%, or100% of the length of the target gene sequence protein coding region,the target gene sequence 5′ UTR region, the target gene 3′ UTR region,and/or any combination thereof. For example, if a target gene sequenceprotein coding region is determined to be 200 nucleotides long, then anisolated dsRNA molecule comprising a nucleic acid sequence complementaryto a contiguous region comprising 95% of the length of the target genesequence protein coding region would be complementary to a contiguousregion 190 nucleotides long.

In certain aspects of any target gene silencing nucleic acid moleculedescribed anywhere herein, including dsRNA molecules for RNAi, thetarget gene comprises one or more of the nucleic acid sequence of SEQ IDNO: 1-14, 16-29, 31-69, 70-75, 76-88, 89-105, and 106-110. In certainaspects, the target gene comprises the nucleic acid sequence of SEQ IDNO: 3 or 14. Thus, in certain aspects, the isolated dsRNA moleculecomprises a nucleic acid sequence complementary to about any of 21, 22,23, 24, 25, 30, 40, 50, 100, 200, 300, 400, 500, 600, 650, 750, 1000 toabout any of 23, 24, 25, 30, 40, 50, 100, 200, 300, 400, 500, 600, 650,750, 1000, or 2000 contiguous nucleotides of a target gene sequencecomprising a nucleic acid sequence selected from the group consisting ofSEQ ID NOs: 1-14, 16-29, 31-69, 70-75, 76-88, 89-105, and 106-110. Forexample, in certain aspects, the isolated dsRNA molecule comprises anucleic acid sequence complementary to about 100 to 1000 or about 200 to1000 contiguous nucleotides of a target gene sequence comprising anucleic acid sequence selected from the group consisting of SEQ ID NOs:1-14, 16-29, 31-69, 70-75, 76-88, 89-105, and 106-110. In certainaspects, the isolated dsRNA comprises a nucleic acid sequencecomplementary to about 200 to 1000 contiguous nucleotides of the proteincoding region of a target gene sequence comprising a nucleic acidsequence selected from the group consisting of SEQ ID NOs: 1-14, 16-29,31-69, 70-75, 76-88, 89-105, and 106-110. In certain aspects, theisolated dsRNA molecule comprises a nucleic acid sequence complementaryto a contiguous region comprising at least about 50%, 60%, 70%, 80%,90%, 95%, 98%, 99%, or 100% of the length of the target gene sequenceprotein coding region, the target gene 5′ UTR region, and/or the targetgene 3′ UTR region. In certain aspects, the isolated dsRNA moleculecomprises a nucleic acid sequence complementary to a contiguous regioncomprising at least about 50%, 60%, 70%, 80%, 90%, 95%, 98%, 99%, or100% of the length of a sequence selected from the group consisting ofSEQ ID NOs: 1-14, 16-29, 31-69, 70-75, 76-88, 89-105, and 106-110.

In certain aspects of any target gene silencing nucleic acid moleculedescribed anywhere herein, the nucleic acid molecule can form siRNA.Thus, certain aspects provide for an siRNA molecule derived from theprocessing of a dsRNA molecule for silencing a target gene disclosedherein.

Insecticidal Compositions

Certain aspects of the disclosure provide for an insecticidalcomposition comprising a nucleic acid molecule disclosed anywhere hereinfor silencing a target gene, including long dsRNA, hpRNA, and siRNA. Incertain aspects, the insecticidal composition also comprises a syntheticcarrier or a microbial conduit. For example, a microbial conduit can bea microorganism that has a natural capacity or is engineered to produceand/or deliver dsRNA to increase its bioavailability and/or biostabilityfor causing RNA interference. Representative examples include plantgrowth promoting organisms, normal commensal and/or symbioticmicroorganisms associated with the target insect pest or pest targethost or host cultivation range etc. from an insect engineered oridentified from natural populations to produce and/or deliver dsRNA. Incertain aspects a microbial conduit can be used as a direct topicalapplication on a whole plant or coated onto a seed or mixed with growthmedia or transmitted through fertilizer or irrigation, etc. In certainaspects, the nucleic acid molecule of the insecticidal composition isconjugated to the synthetic carrier. For example, a synthetic carriercan be an inert chemical compound with a natural or engineered affinityto bind (conjugate) a dsRNA molecule to increase its biostability and/orbioavailability for causing RNA interference. In certain aspects, asynthetic carrier comprises a combination of inert chemicals ornanoparticles that upon combining and/or individually have a netpositive charge or general affinity to bind to negatively charged dsRNA.Representative examples include chitosan, liposomes, carbon quantumdots, biodegradable particles of plant (e.g. coconut coir or grainflour, etc.) or soil (e.g. calcified clay) origin etc. In certainaspects, the dsRNA conjugated with a synthetic carrier can be used as adirect topical application directly and/or after aerosolization on awhole plant or coated onto a seed or mixed with growth media ortransmitted through fertilizer or irrigation, etc. In certain aspects,dsRNA or a composition comprising dsRNA can be used as a direct topicalspray on application to whole plant, coated onto a seed or mixed withgrowth media or transmitted through fertilizer or irrigation or combinedwith plant growth promoting microbes etc.

Recombinant Constructs

Certain aspects of this disclosure provide for a recombinant nucleicacid construct, such as a DNA vector, comprising and/or encoding anucleic acid molecule disclosed anywhere herein for silencing a targetgene, including long dsRNA, hpRNA, and siRNA. Certain aspects providefor recombinant nucleic acid constructs comprising and/or encoding anRNAi precursor of a nucleic acid molecule disclosed anywhere herein forsilencing a target gene, including long dsRNA, hpRNA, and siRNA.

Certain aspects of this disclosure provide for a recombinant nucleicacid construct, such as a DNA vector, comprising a target gene silencingsequence for silencing a target gene described anywhere herein. Incertain aspects, a recombinant DNA construct comprises a gene silencingsequence comprising about any of 21, 22, 23, 24, 25, 30, 40, 50, 60,100, 120, 200, 240, 300, 400, 500, 600, 650, 750, 1000 to about any of23, 24, 25, 30, 40, 50, 100, 200, 300, 400, 500, 600, 650, 750, 1000, or2000 contiguous nucleotides of a target gene sequence disclosed anywhereherein. In certain aspects, a recombinant DNA construct comprises a genesilencing sequence comprising about 100 to 1000 or about 200 to 1000contiguous nucleotides of a target gene sequence. In certain aspects,the gene silencing sequence comprises about 100 to 1000 or about 200 to1000 contiguous nucleotides of the protein coding region of the targetgene sequence. In certain aspects, the gene silencing sequence comprisesabout 100 to 1000 or about 200 to 1000 contiguous nucleotides of the 5′UTR region or the 3′ UTR region of the target gene sequence. In certainaspects, the gene silencing sequence comprises at least 50%, 60%, 70%,80%, 90%, 95%, 98%, 99%, or 100% contiguously of the length of targetgene sequence protein coding region, the target gene sequence 5′ UTRregion, target gene sequence 3′ UTR region and/or any combinationthereof.

In certain aspects, the target gene comprises a nucleic acid sequenceselected from the group consisting of SEQ ID NOs: 1-14, 16-29, 31-69,70-75, 76-88, 89-105, and 106-110. In certain aspects, the genesilencing sequence comprises about 100 to 1000 or about 200 to 1000contiguous nucleotides of a sequence selected from the group consistingof SEQ ID NOs: 1-14, 16-29, 31-69, 70-75, 76-88, 89-105, and 106-110. Incertain aspects, the gene silencing sequence comprises at least 50%,60%, 70%, 80%, 90%, 95%, 98%, 99%, or 100% contiguously of the length ofa sequence selected from the group consisting of SEQ ID NOs: 1-14,16-29, 31-69, 70-75, 76-88, 89-105, and 106-110.

In certain aspects, the recombinant DNA construct has a gene silencingsequence operably linked to one or more promoters for the expression ofa dsRNA molecule that silences the target gene when ingested by aninsect. Thus, in certain aspects, the construct is an expression vector.Representative promoters for use in expressing a dsRNA molecule include,but are not limited to, CaMV35S or ZmUbi1 promoters etc. In certainaspects, the expression vector can target single or multiple insect RNAitarget genes, for example, the vector could comprise one or more genesilencing sequences or could employ multiple vectors to target multipleinsect RNAi target genes or chimeric dsRNA molecules.

Host Cells and Plants

Provided herein are host cells, plants, and plants parts comprising,expressing, processing, and the like a dsRNA as described anywhereherein for inducing RNAi in an insect. In certain aspects, a host cellcomprises a dsRNA molecule, siRNA molecule, a polynucleotide encoding adsRNA molecule, and/or a construct or a dsRNA encoding segment thereofdescribed anywhere herein. Representative examples of host cells includebacterial cells, fungal cells, yeast cells, plant cells, plantorganelles (e.g., including plastids), and mammalian cells. In certainaspects, the host cell is a bacterial or plant cell. In certain aspects,the host cell is a transgenic and/or transplastomic plant cell. One ofordinary skill will understand that there are many well-known methodsfor introducing a nucleic acid, such as a vector, into a host cellincluding well-known methods for generating transgenic and/ortransplastomic plant cells. In certain aspects, the hose cell expressesa dsRNA molecules and/or produces siRNA to silence a target gene. Incertain aspects, a transgenic and/or transplastomic plant can comprise adsRNA molecule, siRNA, a polynucleotide encoding a dsRNA, and/or aconstruct or a dsRNA encoding segment thereof. In certain aspects, atleast one cell of a transgenic and/or transplastomic plant expresses adsRNA molecule and/or produces a siRNA for silencing a target gene.Certain aspects provide for a seed, part, tissue, cell, or organelle ofa plant described herein, wherein said seed, part, tissue, cell, ororganelle comprises a dsRNA molecule and/or the siRNA for silencing atarget gene.

Methods of Insect Control

Also provided for herein are various methods of using a dsRNA moleculeor vector encoding such dsRNA described anywhere herein for inducingRNAi in an insect and/or silencing a target gene. In certain aspects,this provides for control of insect pests.

Certain aspects provide for a method of silencing an insect immuneresponse gene and/or an insect gene encoding for structural componentsof the insect midgut. In certain aspects, a method provides for thesilencing an insect MIGGS-IRTG. Such method comprises providing foringestion through spray, drenches, granules, seed coating orplant-incorporated protectant, or the like, to an insect an isolateddsRNA (pure or crude extract), siRNA, insecticidal composition, hostcell, transgenic and/or transplastomic plant, and/or the seed, part,tissue, cell, or organelle thereof described anywhere herein.

Certain aspects provide for a method of silencing an insect immuneresponse gene and/or an insect gene encoding for structural componentsof the insect midgut. In certain aspects, a method provides for thesilencing an insect MIGGS-IRTG. Such method comprises providing foringestion through spray, drenches, granules, seed coating orplant-incorporated protectant, or the like, to an insect an isolateddsRNA, siRNA, insecticidal composition, host cell, transgenic and/ortransplastomic plant, and/or the seed, part, tissue, cell, or organellethereof described anywhere herein.

Certain aspects provide for protecting a plant, such as a crop plant,from an insect pest including but not limited to pests of the orderLepidoptera like Manduca sexta (tobacco hornworm), Spodoptera frugiperda(fall armyworm), Ostrinia nubilalis (European corn borer), Plutellaxylostella (Diamondback moth) or pests of the order Coleoptera likeLeptinotarsa decemlineata Say (Colorado potato beetle), Diabrotica spp.(Corn rootworm complex), Tribolium castaneum (Red flour beetle),Popillia japonica (Japanese beetle), Agrilus planipennis (Emerald ashborer) or pests of the order Hemiptera like Diaphorina citri (Asiancitrus psyllid), Cimex lectularius (Bed bug) or pests of the orderBlattodea like all species of cockroaches and termites or insect pestsof the order Diptera like all species of Mosquitoes and flies etc.Representative examples of plant hosts include, but are not restrictedto, Zea mays L (corn), Sorghum bicolor (sorghum), Setaria italica (foxtail millet), Pennisetum glaucum (Pearl millet), Solanum tuberosum(potato), Oryza sativa (rice), Lycopersicon esculentum (tomato), Solanummelongena (eggplant), all cultivars of Brassica oleracea family, Citrussinensis (Orange), trees of Oleaceae family and crops of Rosaceae etc.Such methods comprise, for example, topically applying to the plant theisolated dsRNA (pure or crude extract), the siRNA, and/or theinsecticidal composition described anywhere herein, and providing theplant in the diet of the insect pest. In certain aspects the dsRNAmolecule is topically applied by expressing the dsRNA in a microbefollowed by topically applying the microbe onto the plant and/or seed.

Certain aspects provide for producing a plant resistance to a pestinsect of said plant. Such methods comprises transforming the plant witha polynucleotide encoding a dsRNA and/or a construct or a dsRNA encodingsegment describe anywhere herein, wherein the plant expresses a dsRNAand/or siRNA and/or the plant comprises a dsRNA and/or siRNA containinginsecticidal compositions described anywhere herein, for silencing atarget gene. In certain aspects, the transformed plant is more resistantto a pest insect of said plant than untransformed plants.

Certain aspects provide for improving crop yield. Such methods comprisegrowing a population of crop plants transformed with a polynucleotideencoding a dsRNA and/or the construct or a dsRNA encoding segmentthereof described anywhere herein, wherein the plant expresses a dsRNAand/or siRNA and/or the plant comprises a dsRNA and/or siRNA containinginsecticidal compositions described anywhere herein, for silencing atarget gene. In certain aspects, a population of transformed plantsproduces higher yields in the presence of pest insect infestation than acontrol population of untransformed plants.

Certain aspects of the disclosure provide for an insecticidalcomposition comprising a nucleic acid molecule disclosed anywhere hereinfor silencing a target gene, including long dsRNA, hpRNA, and siRNA. Incertain aspects, the insecticidal composition also comprises a syntheticcarrier or a microbial conduit. For example, a microbial conduit can bea microorganism that has a natural capacity or is engineered to produceand/or deliver dsRNA to increase its bioavailability and/or biostabilityfor causing RNA interference. Representative examples include plantgrowth promoting organisms, normal commensal and/or symbioticmicroorganisms associated with the target insect pest or parasitesand/or natural enemies of the target pest or pest target host or hostcultivation range etc. from an insect or parasite and/or natural enemiesof the target pest engineered or identified from natural populationscontaining microbial conduit to produce and/or deliver dsRNA and/ordrive the transmission of such microbial conduits into naturalpopulations of insect pests as a control option. In certain aspects amicrobial conduit can be used as a direct topical application on a wholeplant or coated onto a seed or mixed with growth media or transmittedthrough fertilizer or irrigation, etc. In certain aspects, the nucleicacid molecule of the insecticidal composition is conjugated to thesynthetic carrier. For example, a synthetic carrier can be an inertchemical compound with a natural or engineered affinity to bind(conjugate) a dsRNA molecule to increase its biostability and/orbioavailability for causing RNA interference. In certain aspects, asynthetic carrier comprises a combination of inert chemicals ornanoparticles that upon combining and/or individually have a netpositive charge or general affinity to bind to negatively charged dsRNA.Representative examples include chitosan, liposomes, carbon quantumdots, biodegradable particles of plant (e.g. coconut coir or grainflour, etc.) or soil (e.g. calcified clay) origin etc. In certainaspects, the dsRNA conjugated with a synthetic carrier can be used as adirect topical application directly and/or after aerosolization on awhole plant or coated onto a seed or mixed with growth media ortransmitted through fertilizer or irrigation, etc. In certain aspectsdsRNA or composition comprising the dsRNA can be used as a directtopical spray on application to whole plant, coated onto a seed or mixedwith growth media or transmitted through fertilizer or irrigation orcombined with plant growth promoting microbes etc.

Certain aspects provide for producing a plant resistant against a pestinsect of said plant. Such methods comprise first transforming a plantcell with a polynucleotide encoding the dsRNA and/or the construct or adsRNA encoding segment described anywhere herein. Next, a plant isregenerated from the transformed plant cell. The plant is then grownunder conditions suitable for the expression of the dsRNA. In certainaspects, the transformed plant confers genetically tractable (maternaland/or paternal inherited) gain of function phenotypically manifested asan ability to impair the normal feeding and/or growth and/or developmentand/or reproductive success of the target plant pest and is consequentlyresistant to the plant pest insect compared to a control untransformedplant.

In certain aspects of any of the aforementioned methods, the insectlarvae ingest the dsRNA. In certain aspects of any of the aforementionedmethods, ingestion of the dsRNA induces a melanotic response in theinsect larvae. In certain aspects of any of the aforementioned methods,ingestion of the dsRNA results in perturbation of gut microbialhomeostasis. In certain aspects of any of the aforementioned methods,ingestion of the dsRNA results in defective clearance of opportunisticmicrobes. In certain aspects of any of the aforementioned methods,ingestion of the dsRNA results in defective containment of gut microbes.

One of ordinary skill in the art will recognize that, the inventors havedemonstrated midgut specific expression of a representative number ofMIGGS-IRTGS in TH in response to their feeding on a lab strain ofEscherichia coli (E. coli) bacteria. For example, a set of 20MIGGS-IRTGS (SEQ ID NOs: 1-9, 11, 14, 31, 39, 43, 44, 71-75) that areinduced in TH larvae feeding on an induction medium (Wang et al. (2006).J. Biol. Chem. 281(14): 9271-9278) is disclosed herein. It waspreviously reported that these twenty genes are expressed abundantly inthe gut (Table 1). The gut specific expression of 2/20 genes were testedand validated the same (FIG. 7).

Orthologs of the representative TH MIGGS-IRTG (SEQ ID NOs: 76-88) setwere identified from transcriptomic resources of an economicallyimportant Bt. resistant lepidopteran pest DBM using a combination ofreciprocal best Blast analysis (Ward et al. (2014). PLoS ONE 9(7):e101850) and literature curation. Most of these MIGGS-IRTGS were inducedin response to the feeding of DBM larvae on induction medium (Wang etal. (2006). J. Biol. Chem. 281(14): 9271-9278), similar to observationswith TH larvae.

It was also demonstrated that most of the MIGGS-IRTGS were induced inanother economically important lepidopteran pest, FAW, feeding on plantsgrown on representative field soil, using a RNA-Seq approach.

The RNA-Seq approach also identified additional RNAi candidates (SEQ IDNOs: 89-105) belonging to the MIGGS category. Further, orthologs of theexpanded representative TH MIGGS-IRTG set (SEQ ID NO: 106-110) wereidentified from transcriptomic resources of an economically importantColeopteran pest RFB. It was demonstrated that most of the MIGGS-IRTGSwere induced in response to the feeding of RFB beetles on inductionmedium (Wang et al. (2006). J. Biol. Chem. 281(14): 9271-9278), similarto observations with the order lepidoptera.

It was demonstrated that the targeted silencing of 9 out of 20MIGGS-IRTGS employing bacterially expressed dsRNA protocol (Timmons L.et al. (2001). Gene. 263:103-112.) is insecticidal to TH larvae.Insecticidal activity against TH larvae correlated with the downregulation of target transcripts. It was demonstrated that the targetedsilencing of 7 out of 14 MIGGS-IRTGS using bacterially expressed dsRNAprotocol (Timmons L. et al. (2001). Gene. 263:103-112.) is insecticidalto DBM larvae. It was demonstrated that targeted silencing of 9MIGGS-IRTGS are insecticidal to FAW larvae using bacterially expresseddsRNA protocol (Timmons L. et al. (2001). Gene. 263:103-112.). A coreset of three MIGGS-IRTGS (SEQ ID NO: 3, 4, and 43) was identified thatare efficacious against all three lepidopteran pests TH, DBM and FAW inan orthologous manner and that leaf discs coated with dsRNA against thecore MIGGS-IRTGS are insecticidal against TH, DBM and FAW larvae.Further, plastidal expressed dsRNA against the core MIGGS-IRTGS impactedlarval growth and survival.

The following examples are included to demonstrate certain embodimentsof the disclosure. It should be appreciated by those of skill in the artthat the techniques disclosed in the examples which follow representtechniques discovered by the inventor to function well in the practiceof the disclosure. However, those of skill in the art should, in lightof the present disclosure, appreciate that many changes can be made inthe specific embodiments which are disclosed and still obtain a like orsimilar result without departing from the spirit and scope of thedisclosure.

EXAMPLES

In certain aspects, work was performed towards the identification,induction, isolation and cloning of the selected M. sexta (tobaccohornworm (TH)) MIGGS-IRTGS into a bacterial expression system capable ofenabling the cloned genes to produce dsRNA (Timmons L. et al. (2001).Gene. 263:103-112.). Upon ingestion by M. sexta larvae, the bacteriallyexpressed dsRNA is intended to silence, or at least knock-down, reduce,and the like the corresponding MIGGS-IRTG in order to curtail thefeeding behavior and/or cause lethal effects in the insect pests. Basedupon these results, additional testing was done on other representativeinsect species to demonstrate and establish for purposes of support wideapplicability of the compositions and approaches of the disclosure.

For MIGGS-IRTG selection, PRR type genes co-regulated by the immunedeficiency (IMD) pathway in TH were identified, these genes having beenrecently summarized (Casanova-Torres and Goodrich-Blair (2013). Insects(4): 320-338; Zhong X, et al. (2012). Insect Biochem. Mol. Biol. 42(7):514-524); Zhang X, et al. (2015). Insect Biochem. Mol. Biol. 62:38-50;Cao X, et al. (2015). Insect Biochem. Mol. Biol. 62:64-74; Kanost M R,et al. (2016). Insect Biochem. Mol. Biol 76:118-147). Notably, most ofthe PRR genes selected for this study are abundantly induced orpredicted to express in a midgut specific manner (Pauchet Y, et al.(2010). Insect. Mol. Biol. 19: 61-75; Kim and Lee (2014). Front. Cell.Infect. Microbiol. 3: 116; Lee and Hase (2014). Nat. Chem. Biol., 10:416-424).

The TH-Transferrin, Arylphorin β subunit, chymotrypsinogen-like protein1 and few other immunity related genes do not belong to the conventionalPRR type immune responsive genes. However, these genes were included asthey potentially contribute to the midgut microbial homeostasis throughIMD co-regulation (Pauchet Y, et al. (2010). Insect. Mol. Biol. 19:61-75). Additionally identified were a TH gene indicated to be a valinerich midgut protein critical for the formation of midgut peritrophicmatrix and related genes critical for maintaining the structuralintegrity of the midgut, which are possibly involved, in the gutmicrobial containment. (Odman-Naresh et al. (2013). PLoS ONE 8:e82015.10.1371/journal.pone.0082015; Engel and Moran (2013). FEMS MicrobiolRev. 37 699-735). A non-insect gene that encodes for catalase 1 fromcassava (Manihot esculanta) was used as a control.

Materials and Methods

TH larvae required for isolation of IRTG genes and subsequent bioassayswere reared as follows: Eggs were procured from Carolina BiologicalSciences (Burlington, N.C., USA). The eggs were not surface sterilizedand used directly for conventional rearing (CR). The larval colonyestablishment and maintenance was performed employing a Phytatray II(Sigma, MO, USA) unit containing Gypsy Moth diet. (Gunaratna R T andJiang H (2013). Dev. Comp. Immunol. 39: 388-398).

Bt.-resistant DBM eggs were procured from Benzon Research (Carlisle,Pa., USA) and reared conventionally, as described above.

FAW eggs were procured from Benzon Research (Carlisle, Pa., USA) andreared conventionally, as described above.

For germ free (GF) rearing procedure, all eggs were surface sterilizedwith a solution of Tween-80 (polyoxyethylene sorbitan monooleate),bleach, and distilled water as described previously. (Broderick N A, etal. (2009). Environ. Entomol. 29:101-107). The surface sterilized eggswere transferred to Phytatray II (Sigma, MO, USA) unit containing GypsyMoth diet augmented with antibiotics (500 mg/l each of penicillin,gentamicin, rifampicin, streptomycin). (Broderick N A, et al. (2009).Environ. Entomol. 29:101-107; Gregory R. Richards (2008). Journal ofBacteriology. 190, 4870-4879).

Both CR and GF larvae were reared in an environmental chamber with a16:8 hours (light:dark) photoperiod at 25° C., until use. For theinduction of a representative set of MIGGS-IRTGS of PRR category, 75colony forming units (CFU) of DH5a competent cells (Invitrogen, CA, USA)re-suspended in PBS buffer were injected into healthy 2-3 instar M.sexta larvae. The larvae were snap frozen in liquid nitrogen andprocessed for RNA isolation and cDNA synthesis described herein.

For testing the up-regulation of MIGGS-IRTGS by oral feeding oninduction media, first instar larvae were reared on Luria broth agarmedia plated with a mixture of live E. coli (3×107 cells), M. luteus (30μg), and curdlan (30 μg) in 50 μl of H2O (Wang et al. (2006). J. Biol.Chem. 281(14): 9271-9278).

Total RNA was isolated using RNeasy Mini Kit reagent (QIAGEN, NY, USA)and treated with TURBO DNase (Ambion-Life Technologies, NY, USA) usingmanufacturer's protocols. One μg of DNase treated RNA was used for cDNAsynthesis using iScript cDNA synthesis kit (Bio-Rad, CA, USA). The cDNAwas used as a template for amplifying near full-length transcripts ofthe IRTG. Similarly, a control gene from cassava was also amplified. Fortissue specific cDNA synthesis the control and treatment larvae weresqueezed to isolate hemolymph fraction (HL), dissect midgut (MDG) toobtain rest of the body as described in Pauchet et al. (2010). Insect.Mol. Biol. 19: 61-75). The cDNA template was used for RT-PCR reactionswere appropriate using the SuperScript III One-Step RT-PCR systemfollowing manufacturers protocol (Thermo Scientific; USA).

Transcripts of TH, DBM, and FAW MIGGS-IRTGS and non-insect control geneswere PCR amplified using PrimeSTAR GXL DNA Polymerase (ClontechLaboratories, CA, USA). The PCR reactions were conducted using thefollowing conditions: denaturation at 98° C. for 30 s, annealing at55/60° C. for 30 s and elongation at 72° C. for 45 s, for 35 cycles. ThePCR products were resolved by agarose gel electrophoresis and stainedwith ethidium bromide. The transcripts were gel eluted using QIAquickgel extraction kit (QIAGEN, NY, USA).

Sequence confirmed transcripts were cloned into pCR8/GW vector(Invitrogen, CA, USA) using manufacturer's protocol. The sequenceconfirmed recombinant pCR8 clones were cloned into L4440gtwy using LRclonase enzyme (Inivtrogen, CA, USA). The L4440gtwy is a modifiedversion of Timmons and Fire feeding Vector and was a kind gift from GuyCaldwell (Addgene plasmid #11344). (Timmons & Fire (1998). Nature, 395:854).

For ingestible RNAi bioassays, sequence confirmed MIGGS-IRTG were clonedinto an L4440 feeding vector between two T7 promoters in invertedorientation and transformed into an E. coli bacterial strain carryingIPTG-inducible expression of T7 polymerase, HT115 (DE3). (Timmons & Fire(1998). Nature, 395: 854). Modification of IRTG in this manner waspreviously demonstrated to induce the expression of dsRNA. (Timmons L,et al. (2001). Gene, 263, 103-112; Kamath R S, et al. (2000). GenomeBiol. 2: 1-10.).

The HT115 (DE3) strain is an RNase III-deficient E. coli strain whose T7polymerase activity is IPTG-inducible. The HT115 (DE3) genotype is asfollows: F-, mcrA, mcrB, IN (rrnD-rrnE) 1, lambda -, rnc14::Tn10 (DE3lysogen: lavUV5 promoter—T7 polymerase) (IPTG-inducible T7 polymerase)(RNase III minus), with tetracycline as a selectable marker. (Kamath RS, et al. (2000). Genome Biol. 2:1-10). The standard heat shock protocolfor transformation of L4440::IRTG and control construct was used.

Single colonies of HT115 bacteria containing cloned L4440 plasmids werepicked and grown in a 5 mL LB culture with 50 mg/ml ampicillin (Amp) OnemL of the liquid culture was saved for plasmid isolation followed bysequence confirmation of the L4440::IRTG clone. The recombinantbacterial clones were grown for 8 hours in liquid culture and wereseeded directly on LB plates containing 1 mM IPTG and 50 mg/ml Amp forinducing the dsRNA. (Kamath R S, et al. (2000). Genome Biol. 2: 1-10).Seeded plates were allowed to dry under laminar airflow chamber andincubated at 37° C. temperature overnight.

For larval bioassay three to five 1-2-instar TH, DBM, and FAW larvaewere placed on induced plates containing HT115 (DE3) cells containingthe desired L4440::IRTG. Bioassays were conducted testing the TH larvaeagainst MIGGS-IRTGS and controls listed in Table 1.

TABLE 1 List of total MIGGS-IRTGS from TH tested. Manduca sexta MIGGSNCBI Acession Insect Biological Midgut Insecticidal RNAI Target GenesNumber Function Expression Activity Ms_PGRP2 (SEQ ID NO: 3) GQ293365.1Immunity Yes* Yes Ms_IMD (SEQ ID NO: 31) Msex2.05477-RA ImmunityYes{circumflex over ( )} No Ms_Toll2 (SEQ ID NO: 8) EF442782.1 ImmunityYes{circumflex over ( )} Yes Ms_Sck (SEQ ID NO: 71) Msex2.03324-RAImmunity Yes{circumflex over ( )} No Ms_Akl (SEQ ID NO: 72)Msex2.12479-RA Immunity Yes{circumflex over ( )}{circumflex over ( )} NoMs_Rel2A (SEQ ID NO: 5) HM363513.1 Immunity Yes{circumflex over ( )} YesMs_Spz1A (SEQ ID NO: 47) GQ249944.1 Immunity Yes{circumflex over ( )}Yes Ms_Cac (SEQ ID NO: 73) Msex2.02793-RA Immunity Yes{circumflex over( )} No Ms_Dorsal (SEQ ID NO: 6) HM363515.1 Immunity Yes{circumflex over( )}{circumflex over ( )} No Ms_Cad1 (SEQ ID NO: 39) Msex2.04570-RAImmunity Yes{circumflex over ( )} No Ms_Hemolin1 (SEQ ID NO: 1) M64346.1Immunity Yes++ No Ms_SPH3 (SEQ ID NO: 2) AF413067.1 Immunity DNA NoMs_Transferrin1 (SEQ ID NO: 11) M62802.1 Immunity Yes+++ No Ms_Gloverin1(SEQ ID NO: 74) Gl110649240 Immunity Yes{circumflex over ( )}{circumflexover ( )}{circumflex over ( )} No Ms_HPA18 (SEQ ID NO: 9) AY672794.1Immunity No No Ms_βGRP2 (SEQ ID NO: 4) AY135522.1 Immunity Yes* YesMs_CHS2 (SEQ ID NO: 43) AY821560.1 Structural Integrity Yes* Yes Ms_βTub(SEQ ID NO: 75) AF030547 Structural Integrity Yes+ Yes Ms_Suc1 (SEQ IDN: 44) GQ293363.1 Structural Integrity Yes* Yes Ms_VMP1 (SEQ ID NO: 14)NA Structural Integrity Yes+ Yes Ms_vATPaseE (Positive Control) X67131ATP Hydrolysis Yes Yes Me_Catalase 1 (Negative Control) AF170272 None NoNo

Phenotypic differences in the larval development on L4440::IRTGcontaining HT115 (DE3) plates were documented and compared with thelarval growth on negative and positive controls containing HT115 (DE3)plates. The larval phenotypes for a given treatment with appropriatecontrols were only considered true and documented if they werereproducibly observed in 2/3 or 4/5 larvae, in at least two independentfeeding experiments.

For sprayable RNAi, a 24-well plate-based bioassay system was developedusing a modified cetyl trimethylammonium bromide method of MEGAscriptRNAi kit following manufacturer's protocol (Thermo Scientific, USA) forlarge-scale purification of dsRNA against a given MIGGS-IRTG. Integrityof the dsRNA was determined by electrophoresis on 1% agarose gel and itsconcentration determined using NanoDrop UV-VIS spectrometer. Leaf discsof 1 cm² diameter were detached from Nicotiana benthamiana (for TH) orArabidopsis Col-WT (for DBM) or wheat cultivar Bobwhite (for FAW) plantsgrown on field soil were drop inoculated with 0, 4, 8 or 16 μg ofpurified dsRNA in TE buffer. Air-dried dsRNA coated leaf discs wereplaced in the bioassay plate containing 1 mL of 1% Murashige and Skoogagar medium per well. Each well contained one leaf disc and was infestedwith conventionally reared three first instar TH or DBM or FAW larvae.dsRNA coated leaf discs were replaced once every 24 hours andinsecticidal activity of each dsRNA measured as a function of larvalmortality after five days continuous feeding. The RFB bioassays wereconducted using a previously published flour disc assay protocol (Cao etal. (2018). Int. J. Mol. Sci. 19, 1079) with adult beetles.

For RNA-Seq analysis to test if the MIGGS pathway genes are induced bysoil microbiome in FAW, wheat (Triticum aestivum) seeds were surfacesterilized and planted in 4.5″ pots containing field soil or filled with4:1 sterile turface:sand mix. Seedlings were grown a growth chamber atfor 20 days and infested with ten first-instar FAW larvae per pot.Vigorous larval feeding activity was confirmed and larval samplescollected for RNA-Seq analysis. A “pooled RNA-Seq” approach (Rajkumar etal. (2015). BMC Genomics. 16(1): 548) was used to obtain a snap shot ofdifferential FAW gene expression in response to feeding on plants grownon microbe rich (field soil) and microbe depleted (sterile surface)substrate.

In order to demonstrate additional dsRNA delivery methods a plastidaldsRNA expression system was employed. Since high concentrations of longdsRNAs can be stably produced in plastids (Zhang et al. (2015). Science.347(6225): 991-994), three of the most potent insecticidal THMIGGS-IRTGS (SEQ ID NO: 3, 4, and 43) dsRNAs (dsMsPGRP2; dsMsβGRP2 anddsMsCHS2) were expressed by plastid transformation in tobacco plants incollaboration with Plastomics Inc. following a previously publishedprotocol (Zhang et al. (2015). Science. 347(6225): 991-994). Detachedleaves of stable transplastomic lines expressing dsRNA against MIGGStargets were fed to TH larvae and assessed for insecticidal activity.

Results

Bioassays (FIG. 1) indicated that the oral feeding activity of TH larvaeon L4440::MsPGRP2 and L4440::MsVMP1 containing HT115 (DE3) platesresulted in growth impediment and/or mortality. Contrawise, the larvaegrowing on L4440::MeCAT1 containing HT115 (DE3) plates developednormally without any aberrant phenotypes.

The representative phenotypes of TH larvae at 192 hours post exposure(HPE) to larvae exposed to bacterially (HT115 (DE3)) expressed dsRNAagainst MIGGS RNAi targets MsPGRP2 (FIG. 2A); MsVMP1 (FIG. 2B) andnegative control dsRNA against Cassava plant specific gene MeCAT1 (FIG.2C). The phenotypic effects leading to larval mortality were usuallyassociated with the development of melanotic reaction, reduced appetite,growth and development (FIGS. 2A and B).

Consistent with above, the TH larvae exposed to bacterially expressednegative control MeCAT1 dsRNA displayed vigorous feeding (area betweenthe arrowheads FIG. 3A). In contrast, the TH larvae exposed tobacterially expressed dsRNA against the MIGGS RNAi target MsPGRP2displayed a curtailed feeding (representative picture, area betweenarrowheads FIG. 3B).

In general, melanization is a highly conserved immune response and isoften associated with microbial infection of insects. (Kim S R, et al.(2005). Insect molecular biology, 14(2): 185-194.doi:10.1111/j.1365-2583.2004.00547). The intensification of melanoticresponse in TH larvae upon continued exposure to bacterially expresseddsRNA against the MIGGS RNAi targets MsPGRP2 and MsVMP1 containing HT115(DE3) plates strongly indicates an infection, possibly due to thedefective clearance of opportunistic microbes ingested during feeding.Such defective clearance has been previously associated with theperturbation of gut microbial homeostasis. (Packey and Sartor (2009).Curr. Opin. Infect. Dis. 22(3): 292-301).

Closer observation indicated that the CR larvae feeding on bacteriallyexpressed dsRNA against the MIGGS RNAi targets MsPGRP2 and MsVMP1displayed discernable mortality starting at day 5, reaching up to 100%and 80% mortality respectively, by day 8 (FIG. 4).

To test if both the incidence and intensity of the observed phenotypecould be delayed by clearing gut microbiotas, a GF set of TH larvae werealso subjected to the above treatment with appropriate controls, inparallel. Interestingly, that the incidence of larval mortality was notonly delayed, but also lower in GF larvae in comparison to CR larvae(FIG. 4) was observed.

The incidence of larval mortality on bacterially expressed dsRNA againstMIGGS RNAi targets MsPGRP2 and MsVMP1 plates also correlated with thedevelopment of melanotic reaction (FIG. 5). Most notably, there was aconcomitant delay in the development of melanotic reaction in GF larvaein comparison to CR larvae (FIG. 5). All the phenotypes observed werestatistically significant at a p-value between 0.001 and 0.05. Nosignificant development of mortality or melanotic reaction was observedin larvae exposed to the bacterially expressed negative control MeCAT1dsRNA (FIGS. 4 and 5).

Although MsVMP1 is not directly involved in immune responses, downregulation may abrogate microbial containment, resulting in aninfectious phenotype (FIG. 2B). Alternatively or in addition, downregulation of MsVMP1 may have resulted in wounding of the peritrophicmatrix (the protective lining of the larval midgut) that also may havecontributed to a sepsis mediated infectious phenotype (FIG. 2B); this isfurther substantiated by delayed onset of infectious symptoms in thelarvae (CR or GF) exposed to dsRNA against MsVMP1 in comparison withlarvae exposed to dsRNA against MsPGRP2 (FIGS. 4 and 5). Both defectiveclearance and defective containment of opportunistic microbes have beenpreviously associated with lethal phenotypes. (Packey and Sartor (2009).Curr. Opin. Infect. Dis. 22(3): 292-301). The schematic representationof dsRNA delivery vectors used for above study is indicated in FIG. 6.

Most of the TH MIGGS RNAi targets disclosed herein (e.g., Table 1) areinducible and have been identified from open access midgut specificimmunotranscriptome and/or other datasets (Pauchet Y, et al. (2010)Insect. Mol. Biol. 19:61-75; Odman-Naresh et al. (2013) PLoS ONE8:e82015; Kanost M R, et al. (2016) Insect Biochem. Mol. Biol76:118-147; Brummett et al. (2017) Insect Biochem Mol Biol. 81: 1-9; CaoX, et al. (2015) Insect Biochem. Mol. Biol. 62:64-74); Zhong X, et al.(2012) Insect Biochem. Mol. Biol. 42(7): 514-524; Xia Xu et al (2012).Dev Comp Immunol. 38(2): 275-284). A validation study targeting twoMIGGS RNAi targets MsHEM and MsSPH3 confirmed their preferential midgutspecific manner (FIG. 7) when injected with 75 CFU of gram negative E.coli. Further, literature curation confirmed the abundant expression ofthese MIGGS targets in the insects gut (Table 1).

Oral feeding of TH larvae on induction media containing a mixture oflive E. coli and lyophilized cell wall signatures from gram positivebacteria and fungi (FIG. 8) successfully induced (FIG. 9) the MIGGS RNAitarget genes listed in Table 1. The genes with immunity related functionwere induced between 24-48 hours post larval exposure to induction mediaand mostly not detected in the absence of induction (FIG. 9A-C). While,the genes essential for midgut structural integrity are expressed underboth conditions (FIG. 9D). This, clearly suggests that the MIGGS RNAitargets are induced in response to microbes ingested during feeding.

High-throughput screening of microbe induced MIGGS RNAi targets (FIG. 9)using bacterially delivered dsRNA screen identified 3 insecticidalcandidates (FIG. 10). These MIGGS RNAi targets include Toll receptor(MsToll2), Beta fructofuranosidase 1 (MsSuc1) and Beta tubulin (MsβTub)genes (FIG. 10D-F). The insecticidal activity was manifested as stuntedgrowth and development, loss of appetite and melanotic reaction (FIG.10D-F) as observed with the positive control MsVATPaseE treatment (FIG.10C) in comparison to the negative control treatment (FIG. 10B). Thedevelopmental defects due to the bacterially expressed dsRNA exposureresulted in lethality ranging from 60-70% (FIG. 11), with MsSuc1 beingthe most effective target for killing TH larvae. Mortality rates werestatistically significant and comparable to the MsVATPaseE positivecontrol.

Additionally, a combination of reciprocal best BLAST analysis andliterature curation was used to identify the orthologs of TH MIGGS RNAitargets from the DBM transcriptomic resources (Table 2).

TABLE 2 List of MIGGS-IRTGS identified from DBM transcriptome resources.Midgut Insecticidal Manduca sexta Insect Expression Activity in MIGGSRNAi Biological Putative Plutella Xylostella in Plutella Plutella TargetGenes Function Orthologs Xylostella Xylostella Ms_PGRP2 ImmunityPx_PGRP2 (SEQ ID NO: 76) Yes* Yes Ms_IMD Immunity Px_IMD (SEQ ID NO: 77)Yes* Yes Ms_Rel2A Immunity Px_Rel (SEQ ID NO: 78) Yes* No Ms_Tol2Immunity Px_Toll2 (SEQ ID NO: 79) Yes* No Ms_Cac Immunity Px_Cac (SEQ IDNO: 80) Yes* Yes Ms_Dorsal Immunity Px_Dorsal (SEQ ID NO: 81) Yes* YesMs_Hemolin1 Immunity Px_Hemolin1 (SEQ ID NO: 82) Yes* No Ms_SPH3Immunity Px_SPH3 (SEQ ID NO: 83) DNA* No Ms_Transferrin1 ImmunityPx_Transferrin1 (SEQ ID NO: 84) Yes* No Ms_βGRP2 Immunity Px_βGRP2 (SEQID NO: 85) Yes* Yes Ms_Gloverin1 Immunity Px_Geoverin (SEQ ID NO: 86)Yes* No Ms_CHS2 Structural Integrity Px_CHS1 (SEQ ID NO: 87) Yes* YesMs_βTub Structural Integrity Px_βTUB (SEQ ID NO: 88) Yes* YesMs_vATPaseE ATP Hydrolysis Px_vATPaseE Yes* Yes (Positive Control)Me_Catalase 1 None Ne_Catalase 1 No* No (Negative Control) Manduca sextaNCBI NCBI MIGGS RNAi Accession Putative Plutella Acession Target GenesNumber Xylostella Orthologs Number Ms_PGRP2 GQ293365.1 Px_PGRP2 (SEQ IDNO:76) AFV15800.1 Ms_IMD Msex2.05477-RA Px_IMD (SEQ ID NO: 77) Px103008Ms_Rel2A HM363511.1 Px_Rel (SEQ ID NO: 78) Px102858 Ms_Tol2 EF442782.1Px_Toll2 (SEQ ID NO: 79) Px106338 Ms_Cac Msex2.02793-RA Px_Cac (SEQ IDNO: 80) Px116565 Ms_Dorsal HM363515.1 Px_Dorsal (SEQ ID NO: 81) Px100110Ms_Hemolin1 M64346.1 Px_Hemolin1 (SEQ ID NO: 82) ACN53154.1 Ms_SPH3AF413067.1 Px_SPH3 (SEQ ID NO: 83) XP_004322155.1 Ms_Transferrin1M62802.1 Px_Transferrin1 (SEQ ID NO: 84) BAF36818.1 Ms_βGRP2 AY135522.1Px_βGRP2 (SEQ ID NO: 85) QβVIJ95.1 Ms_Gloverin1 Gl110649240 Px_Geoverin(SEQ ID NO: 86) ACN69342.1 Ms_CHS2 AY821560.1 Px_CHS1 (SEQ ID NO: 87)KX420588.1 Ms_βTub AF030547 Px_βTUB (SEQ ID NO: 88) EU1Z7912.2Ms_vATPaseE X67131 Px_vATPaseE AB189032 (Positive Control) Me_Catalase 1AF170272 Ne_Catalase 1 AF170272 (Negative Control)

It was demonstrated that 14 MIGGS RNAi target genes identified couldalso be induced in Bt resistant strain of DBM feeding on the inductionmedia (FIG. 12), thus suggesting that oral induction of microbeassociated cell wall signatures could induce the MIGGS RNAi target genesin economically important DBM.

High throughput screening of microbe induced DBM MIGGS RNAi target genes(FIG. 12) using bacterially delivered dsRNA screen indicated that 7/15MIGGS RNAi targets tested showed insecticidal activity against a Btresistant strain of DBM (FIG. 13). The DBM insecticidal targets includedPxPGRP2 (SEQ ID NO. 76), PxβGRP2 (SEQ ID NO. 85), PxCHS2 (SEQ ID NO.87), PxCAC (SEQ ID No. 80), PxIMD1 (SEQ ID No. 77), PxDor (SEQ ID No.81) and PxβTub (SEQ ID NO. 88). The insecticidal activity was manifestedas stunted growth and development, loss of appetite and melanoticreaction (FIG. 13D-H) as observed with the positive control MsVATPaseEtreatment (FIG. 13C) in comparison to the negative control treatment(FIG. 13B). The developmental defects due to the bacterially expresseddsRNA exposure resulted in lethality ranging from 53-70% (FIG. 14), withPxPGRP2 the most effective target for killing DBM larvae (FIG. 14).Mortality rates were statistically significant and comparable to thePxVATPaseE positive control (FIG. 14).

In order to test if our MIGGS RNAi technology could work using multipledsRNA delivery platforms we tested if dsRNA against the four TH MIGGSinsecticidal targets could work in a sprayable format. We used dsRNAagainst MsPGRP2, MsβGRP2, MsCHS2 and MsVMP1 for sprayable RNAi assays(FIG. 15) at a concentration of 0, 4, 8 or 16 μg of purified dsRNA in TEbuffer. Most efficacious leaf disc coated dsRNA was 16 μg of dsRNAagainst MsCHS2 that caused 58% mortality. Similar, but slightly reduced,mortality was observed when insects were fed 8 μg of leaf disc coateddsRNA against the same MIGGS targets. Observed mortality rates werestatistically significant and comparable to those observed in TH larvaeexposed to dsRNA against a known positive control target MsVATPaseE(FIG. 16). No statistically significant larvae death was observed whenfeeding on 4 μg dsRNA (FIG. 16), indicating that 8-16 μg dsRNA per leafdisc was required to cause mortality in this assay. In addition todeath, TH larvae feeding on dsRNA were characterized by developmentaldefects, loss of appetite, melanotic reaction and reduced growthcompared to negative controls, indicating significant detrimental impactof MIGGs targeting on insect health (FIG. 17). The insecticidal TH MIGGStargets MsPGRP2, MsβGRP2 and MsCHS2 will be henceforth referred to ascore set.

Similarly, in order to test an additional delivery platform, the coreinsecticidal MIGGS RNAi targets MsPGRP2; MsβGRP2 and MsCHS2 wereexpressed by plastid transformation in tobacco plants in collaborationwith Plastomics Inc. Detached leaves of stable transplastomic lines(FIG. 18) expressing dsRNA against MIGGS targets indicated that the THlarvae feeding on leaves expressing dsRNA against MIGGS targets MsPGRP2(B); MsβGRP (C) and MsCHS2 (D) display stunted growth, development, lossof appetite and melanotic reaction in comparison to negative control(A). The insecticidal activity is manifested as significant reduction inmean weights in comparison to negative control (E). The mortality ratewas scored on a 0-3 score were 0, 1, 2 and 3 indicated ≤0, 25, 50 or ≥50mortality respectively. The transplastomic events confer significantmortality in comparison to negative control (F). Data is average of 6replicates/treatment (N=24) ±SEM at p≤0.001(***); p≤0.01(**) andp≤0.05(*). Clearly suggesting that the stably expressed dsRNA againstthe core MIGGS RNAi targets also impacts larval growth and development.

Next, we tested if our MIGGS RNAi technology can also work in asprayable format against Bt resistant strain of DBM. We used the DBMorthologs of insecticidal TH MIGGS core set (PxPGRP2, PxβGRP2 andPxCHS2) and two newly discovered insecticidal DBM MIGGS targets (PxCACand PxIMD1) for sprayable RNAi assays at a concentration of 0.1, 0.5 and0.25 μg. We observed lethality ranging from 53-67%, with PxPGRP2 beingthe most effective target for killing DBM larvae (FIG. 19). Mortalityrates were statistically significant and comparable to the PxVATPaseEpositive control (FIG. 19). In a manner similar to that seen forsprayable dsRNA against TH, in addition to death, DBM larvae exposeddsRNA treatments were characterized by developmental defects, loss ofappetite, melanotic reaction and arrested growth (FIG. 20). Clearlysuggesting that dsRNA against the insecticidal MIGGS RNAi targetsidentified herein when used in sprayable format is also effectiveagainst Bt resistant strain of DBM.

Most of our MIGGS RNAi target gene induction procedures thus far reliedupon either direct injection or oral feeding of extraneously suppliedmicrobial signatures. To determine if our MIGGS RNAI target genes areinduced under field conditions, we exposed the larvae of economicallyimportant lepidopteran pest FAW to wheat seedlings grown on microbe richand microbe depleted plants (FIG. 21). Pooled RNA-Seq analysis indicatedthat plants growing on field soil caused preferential up-regulation ofMIGGS pathway genes in FAW. In total, 100 differential expressed geneswere identified, thirty of which were MIGGS pathway related genes (FIG.22).

TABLE 3 List of MIGGS-IRTGS identified from using RNA-Seq approach inFAW. Spodoptera fruglperda Putative Insect MIGGS RNAI Biological MidgutInsecticidal Target Genes Query_Contigs_ID* Function Expression*Activity Sf_PGRP1 (SEQ ID NO: 89) rep_c7951 Immunity Yes Yes Sf_Attacin(SEQ ID NO: 90) rep_c9395 Immunity Yes Yes Sf_Ctypelectin15 (SEQ ID NO:91) joint2_rep_c488 Immunity Yes No Sf_Galectin4 (SEQ ID NO: 92)rep_c25253 Immunity Yes No Sf_Lysozyme (SEQ ID NO: 93) rep_c18992Immunity Yes No Sf_Hemolymph proteinase 10 c12881 Immunity No Yes (SEQID NO: 94) Sf_Trypsin like Serine protease rep_c48453 Immunity Yes Yes(SEQ ID NO: 95) Sf-C type Lectin 6 (SEQ ID NO: 96) joint2_rep_c448_Immunity Yes Yes Sf_Serine protease 13 (SEQ ID NO: 97) rep_c1904Immunity Yes No Sf_Cecropin (SEQ ID NO: 98) rep_c42380 Immunity Yes YesSf_Relish (SEQ ID NO: 99) c13122 Immunity Yes No Sf_Toll2 (SEQ ID NO:100) joint2_c3284 Immunity Yes No Ms_βGRP2 (SEQ ID NO: 101) EF641300Immunity Yes Yes Sf_c20042 (SEQ ID NO: 102) c20042 Immunity Yes NoSf_rc16438 (SEQ ID NO: 103) rc16438 Immunity Yes Yes Sf_L2rC2367 (SEQ IDNO: 104) joint2_rep_C2367 Immunity Yes No Sf_CHSB(SEQ ID NO: 105)AY525599 Structural Integrity Yes Yes

Most notably, FAW orthologs of TH insecticidal targets including PGRP2,βGRP2 and IMD were captured in the data set. This discovery indicatedthat MIGGS pathway genes are up regulated in response to insect feedingon plants exposed to microbes in the field soil.

Preliminary experiments were performed to screen the insecticidalactivity of 17 FAW MIGGS RNAi targets discovered during RNA-Seq (Table3, above) in which dsRNA against the FAW orthologs of insecticidal THMIGGS core set (SfPGRP2 (SEQ ID NO. 89), SfβGRP2 (SEQ ID NO. 101) andSfCHS2 (SEQ ID NO. 105) and three newly discovered MIGGS targets SfCTL(SEQ ID NO. 96), SfRC (SEQ ID NO. 103) and SfGAL (SEQ ID NO. 92) fromRNA-Seq were fed to FAW larvae at 0, 4, 8 or 16 μg-purified dsRNA in TEbuffer. FAW 1st instar larvae were allowed to feed on dsRNA coatedleaves following the bioassay described for TH above. Data indicatedthat FAW larvae (FIG. 23) exposed to pure dsRNA against SFCHS2 (B);SFβGRP2 (C); SFβGRP2 (D), SFRC (E), and SFCTL (F) causes reduced growth,development and loss of appetite in comparison to negative controltreatment (A) resulting in significant weight reduction (G) andmortality (H) at 8 and 16 μg of dsRNA concentration. The rates ofmortality was scored on a 0-3 score were 0, 1, 2 and 3 indicated ≤0, 25,50 or ≥50 mortality respectively. The dsRNA treatments imposed causedstatistically significant reduction in mean weights (G) that alsotranslated into significant rates of mortality (H) in comparison tonegative control (FIG. 23). Screening of insecticidal activity ofadditional MIGGS RNAi target genes identified from the RNA-Seq datasetindicated that 16 μg of leaf disc coated dsRNA against MIGGS RNAitargets SfTSP (SEQ ID NO. 95), SfAtta (SEQ ID NO. 90), SfCec (SEQ ID NO.98) and SfHp10 (SEQ ID NO. 94) caused statistically significantreduction (FIG. 24) in mean weights (A) that also translated intosignificant rates of mortality (B) in comparison to negative control.Data is average of 3 replicates/treatment ±SEM at p≤0.001(***);p≤0.01(**) and p≤0.05(*).

Experiments with an economically important coleopteran pest RFB werealso conducted to test if MIGGS-IRTGS targets identified by acombination of reciprocal best BLAST analysis and literature curation(Table 4) are insecticidal against the order coleoptera.

Preliminary feeding trails with 1 μg of purified indicated that dsRNAagainst RFB MIGG RNAi targets TcPGRP2 (SEQ ID NO. 107), TcβGRP2 (SEQ IDNO. 108), TcMDGP (SEQ ID NO. 109) and TcCHS2 (SEQ ID NO. 110) isinsecticidal to the adult RFB beetles. Significant rates of RFBmortalities were observed (Figure. 25) when scored on a 0-3 scale were0, 1, 2 and 3 indicated ≤0, 25, 50 or ≥50 mortality respectively incomparison to the negative control treatment. Data is average of 3replicates/treatment ±SEM at p≤0.001(***); p≤0.01(**) and p≤0.05(*).

TABLE 4 List of MIGGS-IRTGS identified from RFB transcriptome resources.Spodoptera frugiperda Insecti- MIGG RNAI Target Genes Putative InsectMidgut cidal (SEQ ID NO) Query_Contigs_ID Biological Function ExpressionActivity TcPGRPL0 DT786101.1 Immunity Yes* No (SEQ ID NOL: 106) TcPGRP2XM_965754.3 Immunity Yes* Yes (SEQ ID NO: 107) TcβGRP2 XM_966587.4Immunity DNA Yes (SEQ ID NO: 108) TcMDGP XM_971351 Structural IntegrityYes** Yes (SEQ ID NO: 109) TcCHS2 EFA 10719.1 Structural IntegrityYes*** Yes (SEQ ID NO: 110)

One possible reason for larval mortality could involve down regulationof MIGGS-IRTGS transcripts upon feeding on exogenously supplied dsRNAagainst the target genes. Preliminary RT-PCR data indicated that thelarval phenotypes (FIG. 17) also correlated with down regulation oftarget transcripts (FIG. 26).

Importantly RNA-Seq analysis indicated that the MIGGS-IRTG pathwaytargets are induced by soil microbiome indicating that our novel RNAiapproach could be effective even under field conditions.

Given the ease of identification, high specificity, and applicability todiverse pests and delivery platforms, RNAi silencing of the MIGGS-IRTGpathway genes identified, this approach offers an unprecedentedpotential as a novel pesticidal strategy.

The translation of these preliminary findings into a pesticidal RNAitechnology against economically important pests might lead tosustainable alternatives including but not restricted to the methodsdescribed anywhere herein.

Additionally, the proposed approach will shed more light intounderstanding the tri-trophic interaction between plants-microbe-insectinteractions as it pertains to sustainable insect pest protection.

SEQUENCES

SEQ ID NOs: 1-14 and 31-44 are representative examples of M. sexta-RNAitarget gene sequences.

SEQ ID NO: 15 is non-insect gene sequence that encodes for catalase 1from cassava (Manihot esculanta).

SEQ ID NOs: 16-29 are coding region sequences of representative M.sexta-RNAi target genes.

SEQ ID NO: 30 is the coding region sequence of catalase 1 from cassava(Manihot esculanta).

SEQ ID NOs: 45-70 are 5′UTR and 3′UTR region sequences of representativeM. sexta-RNAi target genes.

SEQ ID NOs: 71-75 are the coding region sequences of additionalrepresentative M. sexta-RNAi target genes.

SEQ ID NOs: 76-88 are the coding region sequences of representative P.xylostella-RNAi target genes.

SEQ ID NOs: 89-105 are the coding region sequences of representative S.frugiperda-RNAi target genes.

SEQ ID NOs: 106-110 are the coding region sequences of representative T.castaneum-RNAi target genes.

SEQ ID NOs: 111-119 are representative examples of Manduca sextainsecticidal dsRNA sequences.

SEQ ID NOs: 120-126 are representative examples of Plutella xylostellainsecticidal dsRNA sequences.

SEQ ID NOs: 127-135 are representative examples of Spodoptera frugiperdainsecticidal dsRNA sequences.

SEQ ID NOs: 136-139 are representative examples of Tribolium castaneuminsecticidal dsRNA sequences.

> M. sexta-Hemolin (MsHEM); M64346.1 (SEQ ID NO: 1)ATGGTTTCAAAAAGTATCGTCGCTTTGGCTGCGTGCGTCGCAATGTGCGTAGCCCAGCCAGTGGAGAAGATGCCTGTGCTGAAGGACCAACCCGCTGAAGTCCTCTTCCGGGAGTCTCAGGCCACCGTTCTCGAATGTGTTACCGAGAATGGCGATAAAGATGTCAAATATTCTTGGCAAAAAGACGGCAAAGAATTCAAATGGCAGGAACACAATATCGCCCAGCGCAAAGACGAAGGCAGCCTGGTCTTCCTCAAGCCCGAGGCTAAAGATGAAGGCCAATACAGATGTTTCGCTGAGTCGGCCGCCGGAGTCGCCACCTCCCACATCATCTCCTTTAGAAGGACCTACATGGTCGTACCTACTACTTTTAAGACTGTAGAAAAGAAACCGGTAGAAGGGTCATGGCTCAAACTTGAGTGCAGCATCCCCGAAGGTTATCCTAAACCTACTATTGTATGGAGAAAGCAGCTTGGTGAAGACGAAAGTATAGCAGATTCTATACTGGCACGTCGTATTACACAATCTCCAGAGGGAGACCTGTACTTCACGAGCGTCGAGAAAGAAGACGTAAGCGAAAGCTATAAATACGTTTGCGCTGCTAAGTCACCGGCTATTGATGGGGATGTCCCTCTTGTTGGATACACTATTAAAAGCTTAGAAAAGAATACAAATCAGAAAAACGGTGAGCTGGTCCCGATGTACGTCAGTAATGATATGATAGCTAAGGCCGGAGACGTTACTATGATCTACTGCATGTATGGTGGAGTCCCAATGGCTTACCCCAACTGGTTCAAAGACGGTAAGGACGTGAACGGCAAACCGAGCGACCGCATCACCCGCCACAACAGAACCTCCGGCAAAAGACTGTTCATCAAGGAGACGCTGCTCGAAGATCAGGGCACTTTTACTTGCGACGTGAACAACGAAGTCGGCAAGCCACAGAAACATTCCGTCAAACTTACCGTAGTCAGTGGACCCAGATTTACGAAGAAACCAGAAAAGCAAGTCATCGCTAAGCAGGGCCAGGACTTTGTAATCCCCTGTGAAGTATCCGCCTTACCGGCCGCCCCTGTCTCCTGGACGTTCAACGCCAAGCCCATCAGCGGCAGCCGCGTGGTAGCCAGCCCGAGCGGACTGACCATCAAGGGCATCCAGAAGTCTGACAAGGGTTATTATGGCTGCCAGGCCCACAACGAGCACGGAGATGCCTACGCTGAGACGCTCGTGATTGTTGCTTAA> M. sexta-Serine proteinase homolog 3 (MsSPH-3); AF413067.1(SEQ ID NO: 2)ATGTTGTTGCTTCTGTATTGTCTTGTGGCGGCCTCCGCGCCGTTCTTTATTGCAGCGGACCAAGGCAGCCCTGACCTGCCTTTAGCTACCGAACCACCAACAGAATGCGGAACAATAGCACCTGATGATAGCTTAGTATTAGATGGGTCCGTTGGTAAAAGTGACAAATTACCTTGGTATGCTATTATCTACACAACCACCACCCGGCCATACAAGCAGATCGGTGGAGGAACCCTCATCACTCCTTCAGTAGTAATCTCAGCCGCTCACTGTTTCTGGCGCAATGGTGAGGTTCCATCTAAGGATAATTACGCCGTGGCGCTCGGCAAGACCCATAGTGCTTGGAATAGCCATGCCGATGTAAACGCTCACAAGTCTGATGTAAAAGAAATACACATACCACCGCAGTTTAAGGGAAGGAACACTAATTATCGGAATGATATAGCAATCGTGGTCATGTCAGACCCTGTGACCTACAAAGTGGACATCCGCCCTATCTGTTTGAACTTCGATGTACAATTTGAAAGACTGCAATTAAAAGACGGCATTATGGGGAAGATCGGCACATGGAATGTAAGTCGTGAGACACTGAAACTATCGAAAACATTAAAAGTGGTGGAGAATCCATACATTGACGCAGCGACTTGTATTAGTGAGTCTCCGGCAAGCTTCAGAAATTCCATCACTGCGGACAAAATATGCATCGGATACGTTAACGGCACCGGGCTATGTAGAGGTGATGGCGGCGCTGGGGTGGCTTTCCCTAGCCAGGAACAAGGAGTGCAACGTTACTACCTCAGAGGTGTTATATCTACAGCCCATACCAGCGATGATGGCAACTTATGTGCAGATGGATTTGTAACTGCCACTGCTATAGGCCATCACGAACATTTTATCAAACAGTTTATAAGCGTTTAG> M. sexta-Peptidoglycan recognition protein 2 (MsPGRP2); GQ293365.1(SEQ ID NO: 3)ATGGCGAGCTTCGCTTTAATAGTTATCCTTAGCGTAATTGGCTTTATATCGGCCTATCCTAGTCCTGAAGGTTACAGTTCTGCCTTCAACTTTCCATTCGTAACCAAGGAGCAGTGGGGCGGCAGGGAGGCACGCACGTCGACGCCACTCAACCACCCAGTGCAGTTCGTGGTGATCCACCACAGTTACATTCCCGGCGTGTGCCTCAGCCGGGACGAGTGCGCGCGCAGCATGCGCTCCATGCAGAACTTCCACATGAACAGTAACGGGTGGAGTGATATTGGATACAACTTCGCTGTCGGCGGTGAAGGGTCGGTGTACGAGGGCCGCGGCTGGGACGCGGTCGGCGCACACGCAGCTGGCTATAACAGTAACAGTATCGGCATCGTGCTCATCGGCGATTTTGTTTCAAACCTCCCGCCGGCGGTGCAAATGCAAACCACACAAGAATTGATCGCAGCGGGCGTGCGACTCGGTTACATCAGGCCCAACTACATGCTCATCGGGCATCGTCAGGTCTCCGCCACTGAGTGCCCAGGAACCAGACTCTTCAACGAAATCACCAACTGGAACAACTTCGTGAGGATATGA> M. sexta-Beta-1, 3-glucan-recognition protein 2 (MsβGRP2); AY135522.1(SEQ ID NO: 4)ATGTGGATCAAGAGCGTCTGTTTGTTCGCAACCATTGCGGGCTGCTTGGGCCAGCGAGGGGGTCCATACAAGGTGCCTGATGCGAAACTCGAAGCTATCTACCCCAAAGGCTTGAGAGTCTCTGTGCCAGATGATGGCTACTCCCTATTTGCCTTCCACGGCAAGCTCAATGAGGAGATGGAAGGTTTAGAGGCTGGCCATTGGTCCAGAGACATCACCAAAGCGAAGCAGGGCAGATGGATATTCAGAGATAGGAATGCTGAGCTGAAGCTTGGAGACAAAATTTACTTCTGGACTTACGTTATTAAGGATGGATTGGGATACAGGCAGGACAATGGAGAATGGACTGTTACAGAATTCGTCAATGAGAACGGTACAGTGGTGGACACTAGTACAGCGCCGCCACCAGTAGCACCCGCCGTTTCAGAGGAAGATCAATCGCCAGGTCCTCAGTGGAGACCTTGCGAAAGATCCCTGACTGAGTCCTTGGCCCGCGAACGCGTTTGCAAAGGCAGCCTTGTCTTTAGCGAGGACTTTGATGGTTCCAGTTTGGCCGACTTGGGCAATTGGACCGCTGAAGTCAGATTCCCTGGCGAACCGGACTACCCGTACAACTTGTACACTACGGACGGCACTGTGGGATTCGAAAGTGGGTCTCTGGTGGTGAGACCCGTCATGACCGAGTCCAAATACCACGAGGGCATCATATACGACCGCCTCGACCTTGAGAGATGTACAGGACAGCTGGGTACGCTGGAATGCAGGCGAGAGAGCAGCGGCGGTCAGATTGTACCACCTGTGATGACAGCTAAACTGGCCACTCGACGCAGCTTCGCGTTCAAGTTCGGCAGGATCGATATAAAGGCGAAGATGCCGCGCGGGGACTGGTTGATACCAGAACTCAACCTCGAACCTTTAGATAACATATACGGCAACCAGCGATACGCTTCGGGTCTCATGCGGGTCGCGTTCGTGAGAGGAAACGATGTATACGCCAAGAAGCTCTACGGAGGTCCGATAATGTCCGACGCGGACCCGTTCAGGTCCATGCTGTTGAAGGACAAGCAAGGGTTGGCCAACTGGAATAATGATTACCACGTCTACTCGCTGCTGTGGAAGCCTAACGGTTTAGAGCTGATGGTGGACGGTGAAGTGTACGGCACCATCGACGCTGGCGATGGCTTCTACCAGATTGCGAAGAACAACCTCGTGAGCCACGCCTCGCAGTGGCTCAAGGGCACCGTCATGGCGCCGTTTGATGAAAAGTTCTTCATCACTCTGGGTCTTCGCGTGGCGGGTATCCACGACTTCACGGACGGTCCGGGCAAACCTTGGGAGAACAAGGGCACCAAGGCCATGATCAACTTCTGGAACAATCGGTTCCGCTGGTTCCCCACGTGGCACGACACCAGTCTTAAAGTCGACTACGTCAGAGTCTAT GCTCTTTAG> M. sexta-Relish family protein 2A (MsREL2A); HM363513.1 (SEQ ID NO: 5)ATGTCCTCTTGTCCAAGCGACTATGATCCCAGTGAATCGTCCAAATCTCCACAAAGTATTTGGGAGTCAGGAGGATACAGTTCTCCGTCGCAACAAGTTCCTCAATTGACTTCTAACTTAACAGAATTGTCTGTTGATCACAGCTATAGATACAATGGAAATGGACCATATCTACAGATCACAGAGCAACCACAGAAATACTTTCGGTTCCGTTATGTTAGCGAGATGGTGGGAACACATGGATGTTTGCTTGGCAAATCTTATACAACAAACAAAGTTAAAACTCATCCGACAGTTGAACTCGTGAATTACACCGGTCGAGCCCTGATAAAGTGCCAACTATCGCAAAACAAGAGCGAAGACGAACACCCGCACAAACTGCTCGATGAACAAGACAGAGACATGAGCCACCACGTTCCCGAGCACGGCAGTTATAGAGTGGTATTTGCTGGTATGGGTATAATTCATGCTGCCAAAAAGGAAGTTGCGGGGTGGCTCTATAGAAAATATATACAGCAGAACAAGAATGAAAAGTTTAATAAGAAAGAGCTCGAAGCGCATTGTGAGAGGATGTCCAAAGAGATCGATTTAAATATAGTTAGACTGAAGTTTAGCGCTCACGATATTGACACTGGCATTGAAATTTGCCGGCCAGTGTTCTCTGAACCCATTTATAATTTGAAGTGTGCGTCTACGAATGATTTGAAAATATGCCGCATAAGCCGTTGTTACGGTAGACCGAGAGGCGGCGAAGATATCTTCATATTTGTCGAAAAGGTCAACAAGAAAAACATCCAAGTTCGGTTCTTTAGACTGGAAAACGGGGAGCGCACCTGGTCAGCGATGGCGAACTTTCTGCTAAGCGATGTTCACCACCAATACGCTATCGCTTTTAGAACGCCACCGTACGTCAATCACCAAATTTCTGAAGACGTGCAAGTTTTTATAGAACTCGTACGCCCTTCAGACGGTAGGACGAGCGCTCCCATGGAGTTCACATACAAGGCTGAGCAAATCTATAAACAGAACAAGAAACGTAAAACTACTTCGTCGTACTCGTCGCTCGACAGCTCCTCAGGTTCGGCCGGTTCAATTAAAAGCATCAGCGAACTGCCCGCGCCCGTTGTTTTTGCTGAAAACGTAAGTTTTTTCTATGACACATTACTCATTCTTCAACCCATGACGAATCTATAA> M. sexta-Dorsal (MsDor); HM363515.1 (SEQ ID NO: 6)ATGCTTGTGACGTTATGCGGCGGGAACTATAGTGGATTGTCGTTAACAAAAACTAATCATTATATGTCACCAAAATCATATGTGCCAGGAAATGGTTATGACGCCGCCGTAATCCTAGGTACCACGGAGCAGAATGACAGCGAACCCTCAAACTTGAATATTAGTGATGTTTTTGAAGCCATCACGCTCGCTGATCCGTCGTTCGGCGCGGGCGTGCCGTCGGTAGAGGAGACGATGGCGCACACGCAGCCCCAGCCGCTGCAAATGCCGTACGTGGTCGTCGTGCAGCAGCCCGCCAGCAAAGCGCTCAGATTTCGATATGAGTGCGAGGGCAGATCAGCCGGTTCGATTCCCGGCGCGTCGAGCACGCCCGAGAACCGAACCTTCCCCGCCATCAAGATAATCGGCTACACCGGCACCGTCTCCATCGTAGTGTCGTGTGTCACCAAAGATGAGCCTTGCAGGCCGCACCCACACAACCTGGTCGGGCGCGACCACTGCGACCGCGGCGTGTTCTCCGTCCGCATCGAGATCACCGACGAGAATAACGAATACCAGTTTCGGAACCTGGGCATACAGTGCGTCAAGCGGCGCGACATCGGCGAGGCGCTGCGGATCCGAGAGGACCTGCGCGTCGATCCGTTCAAAACCGGCTTCACCCACCGGAACCACCCGCAAGGCATCGATCTGAATGCAGTGCGGCTCGCGTTCCAAGTGTTCCTGCCGCACTCCAGCGGCAAGATGCGGCGCACGCTCGCGCCCGTCGTGTCCGACGTCATCTACGACAAGAAGGCCATGAGCGACCTGCTCATCGTGCGCGCGAGCCACTGCGCCGGCCCGGCGCGCGGCGGCACGCAGGTCGTACTGCTCTGTGAAAAGGTGACTCGCGAGGACACCGTGGTGGTGTTCTACCAGGAGGACAACAACCGCGTGCTGTGGGAGGAGATGGCGATCATCATCGTGGTGCACAAACAGTATGCCATAGCGTTCGAGACGCCGCCATACAAGAACCCAAACATTACTGATAATGTCAATGTACGATTCCAGCTGAGAAGGCTCTCCGACAAGATGACGAGCAACTCGCTGCCGTTCGAGTACATTCCCGAATACCAAGATTACCCTAGTTACAGGCAGGATAACTCAGAAAGAAATCCCCAATCGCAGCCAATTACGCACAAGGTAACGGTGGAGGACTTTGAATCGACAACTAAAAGATATTTTACGCGAAGCACTGACAACAGTAATTACGGTTGGGATGCGGTTCCGGTCACGTACAATGGAAGAAAGAAGGTTTGCTATTGCCCCAAGAGGAGCTAA> M. sexta-Spätzle (MsSPZ1A); GQ249944.1 (SEQ ID NO: 7)ATGGCCTGGATCCAGCATTTACTCGTTTGGCTCTTCGTTATGTCAACATCAGCATACAAATGCAAAGACTGCTTCAGTTTCGCATCACAATATCCGTCGTACGATAGTCAAGTATACGAACAACCTGACAGACGGATAGCGGGACGGTCAGCACAATACGAACATTTAAGAACAAACGAGAGGTCTCTCCCGGTCTACAGCGAGACCCAGAGGATACAAGCAGAAGAGAGAAGAAGACACAGTTCGAGACTAGAAGAACCGAGACAACGTGCTGAGAATGGTTCATATAAGATATTGAATAACCCTCCGAAACCCTGTATTACTAATAGGAGAAGTCAAATTGATTCGTCGAATGATAGGGTAGTGTTCCCCGGTCCGACTTCAGAAAGGTCGTACGTACCCGAAGTGCCAGAGGAATGCAAGAAAATCGGCATATGCGACAGTATACCGAATTACCCAGAAGAACACGTAGCTAATATTATATCTCGACTTGGAGACAAAGGAAAAGTATTACAAATAGACGAACTGGACGTATCAGACACTCCAGATATCGCCCAGAGGTTGGGTCCGCAGGAGGACAACATGGAACTATGTAGCTTTAGAGAAAAGATTTTTTACCCCAAGGCAGCGCCAGACAAAGATGGAAATTGGTTCTTCGTTGTGAATTCAAAAGAAAACCCAGTACAGGGTTATAAAGTTGAAATTTGCGACCGTCAGCAATTACCATGCGCGGAGTTCGCGAGCTTCCAACAGGGATATGAAGCGAGGTGCATCCAGAAATACGTTCGCCGGACCATGTTGGCGTTGGATCCCAAGGGTCAGATGACCGACATGCCCCTTAAAGTGCCCAGCTGTTGCTCATGCGTGGCCAAATTGACAATCATATGA> M. sexta-Toll receptor (MsTOLL); EF442782.1 (SEQ ID NO: 8)ATGCAGGCTCGGCGGTGGTGCGCGGCACTGCTATTAATGCAGATGCTGAGCTGGCTCGGAGTCAGTGGACACTTACCGCGTCCCGAGTGCGCGCCAGCCGCAGATTGCCAACTTATACGAGACAACATAATCGATGGATATGCACAATTCTACTTCAACGTATCAGGACATGAAGTGAAATTTGAACATTACATCGGAAACGACTTCGATGTCGAATTGTCATGCAATTACATCGCCATGGACAACGCAATGCTGCCGCGGTTCTCAACGACCTTTTCAGTCAACGTAATAGTGGTTAAAGAATGTGCTTTGCCAAGAAGTGGGTCAATCGATGCCGCTGTCGCTGCACTTAATATCAACGTTTTGACGGAGCTGACTCTGGACAAATTCCTAGAGCCGGCGGTGATCACGCGCGCACATCTTACCAGTTTACAACGACTAGAGAGGCTGGAGCTACACGGTAACTCAAACACAAGCCTCGCCCCCGGCGCACTGGCCGCGCTCTCCGCCGCGTCCGCACTGAAATGTCTTGTATTGCATGCAGTACGCGTGCCCGCCGCTGACCTGGCGCGCTTGCCGTCGTCACTGCAAGAACTAGCGTTGTTGGATGTGGGCGCTGCGAGTATGCATTTAGATTCATCGGTTAATTTGACGTCACTCTTCGTAATCGATACACATTATCCTGTCGTCGTGAATGTGAGCAACGCCGTTGCGCTCAGAGACTTGCACATAAATACCCCAAGTACTGTGTTGACCGAAGACGTGCTCCCGTCGTCACTCAACTCACTTGAACTAGAGGGGTGGAACGAAACGCATCCGGTGCCTAAGACACGTTGTGTACTACTTAAGGAACTTAATGTAATCGGCACCGACAATGATGCCTATCCGGTGACTCTCCCGGACGAGTGGCTGTCCAACTGCGGACAGCTGAGGGATCTTGAAATGATCTCCGTGCCGATTAGCGCCGTACTTCCGGCGCGGATGCTGGCTAACGCAATTAGGCTTGAAACGATTACTATCTGGAACTGTAACCTGACCGCGTTGCCGTCGGGCCTGTTAGACGACACGCTGAACCTCGCCACACTCGACTTGTCCAACAATCAACTTGCATCGTTGCCCAGAAAATTATTTGAGCACACGAAGTTACTACGCAGTCTCATACTATCGAACAATCAGCTGACGAGCGAGGTAGTGTGGACGCTGTCGACCGTCACCTCGCTTGTTGAACTAAAGCTCAGCAATAATAACCGCATAGGCGACTTATGTTCCCACGACTCAGTGTCAGCAGGTCCCTCACCACTGAGTTCACTGACGGGGCTAAACTATCTCCATCTGAGCAACACAGGAGTATCACACGTGTGCTCCGACTGGCGAGAAAAGCTAACCTACCTCACGAATCTCAACTTAAGGGACAACCCCATCACTCTCTACAATTTGGCGGATCTACAGTTTCGTCGAATCTGGGTGGACGCTCGTGTGTATTTAGGACATTTCAAGCAGCAGTTTACGCGCACAGATTACGAACTCGCCAGTAATAATAACACTGAGGCGGTCGTAACTTTATCCGGTTCGTTAGAATGCGACTGCAATTCATACTGGGCAGCACAGGTGTTCCGTATGAAAGCTTGGCAGGCATCCAGCTCCATGATATATTGTGAAAAAAAACCGGTCATTGAGGTGGATCCCGACACCTTTACCTGCCTTGAGCCAGCGAAGTGTGCTGCGCTGGCGGATAGTTGTACGTGCCGTATCCGCGACGACATTCAGTACAAACAAGTGGTCGTTGTGCACTGCACCGGACTCGCCGAGTTCCCGCGCCTGCCACTAACCACGGACAAGTGGATCCTGCACCTGCCCCACAACAACATATCATACCTCGCCGCGGCCGACGTATCGCCGAACATCGTGGAACTCGATCTAAGAAACAATTCAATCAAGAATATCGACGTACAGGCATCAGCAAAACTAGCCTTCGTCCGGCTGCAATTGGGTGGTAACCCGATCGAGTGTGACTGCGAGGCGTTGAAGCTGCTGGCGCCGCTGCTCAAACCTGACTCAAAGCTGCTTGACAGAAAGGACGTGAAATGTGAGAACGACGCGCAGATTACCTTGGCGATGCTGAAATTATGTACTAAGTCATCCAATGGGCTGATGTACTTGCTGTTCCTGTTGCTGTTACTCGCATTTGTCGTAACCGGACTGCTCGCACGAACCGCAATTCGCCTGCGCATCAAAATGATCCTCATGAGACTGGGTTGGATGTCGAGACTACTGGAGCCCGCGGACGACGATCGCCCGTACGACGCGTTTGTGTCTTTCGCACACGAGGATGAGGAGCTGGTGATGGAGCAGCTGGCGGCACGGCTCGAGAGCGGCTCGCGGCCGTACCGACTGTGTCTGCACTACCGCGATTGGGCGCCAGGCGAGTGGATCCCGGCGCAAATAGCGGCTTCGGTGCGGGCCTCTCGGCGCACGGTGGCGGTTGTGTCGGCGCACTACTTACAGTCGGGCTGGGCGCTTGCCGAGATCCGGGAGGCGACCGCAGCCTCGCTGCAGGAAGGCATGCCACGTCTCATCATCGTGCTGCTCGACGAGACCGACCGGTTGATGCTCGATATAGACCCTGAGTTGCACGCCTATGTGCGCAACAATACCTACGTGCGCTGGCATGATCCATGGTTTTGGGAGAAGCTGAAGCAGGCGCTGCCTCCACCGCGGGAACAACGGTCGCCAATAGCTCCGTCGCTACCAGCGCTGGCGCTGTCCCATGACAGCCTAACTCTGCGCACGTACTCTCCCAGAGAAAGTGACCCAGCGCCCGCTGCTAAGCCGGCGCACACTCCGCACGAGACGGACGAGCCAGCGCCGGGCGCGAGTCCTTGCTACAAATGA > M. sexta-Scolexin A (MsSCA1); AF087004.1 (SEQ ID NO: 9)CGGCAGTCGGTTGTGTTGGCAGTGGCGGCGGTGCTCTTCGGGTGCGCGTGCGCAGCGCCCAATCCTGGCGCCAACGACATACAACTTAATCAAAAATTAAGTATCGAAGCTAAGGGGGCAAAGCAGCCAATTGATACGAGGGCAGTGAAGGAACGGTATCCATACGCAGTTCGGAGTTTCGGAGGCTTCTGCGGAGGAACCATTATCAGTCCCACCTGGATCCTGACCGCCGGCCACTGCTCGATACTCTATGCGGGGAGCGGCCTACCGGCCGGCACCAACATTACCGAGGTATCTAGCTTGTACCGCTTCCCCAAGCGGCTCGTCATACACCCGCTCTTCTCCATAGGACCCGTCTGGCTCAACGCTACGGAGTTCAACCTCAAACAGGCGGCTGCACGATGGGACTTCTTGTTGATAGAACTGGAGGAACCGCTGCCGTTGGACGGCAAGATCCTGGCGGCTGCGAAGCTCGACGACCAGCCCGACCTCCCCGCAGGCCTCGACGTGGGCTATCCGAGCTACAGCACCGACACCTACGAGGCTAAGATACAAAGCGAGATGCACGGAAAGAAGCTTTCGGTTCAATCTAACGAGGTGTGCTCGAAGCTAGAGCAGTTCAAGGCGGAGGACATGTTGTGCGCCAAGGGACGTCCACCGCGATACGACTTCGTCTGCTTCAGCGACAGTGGCAGTGGGCTAGTAGACAACAATGGTCGCCTAGTCGGCGTGGTGTCGTGGGCCGAGAACAACGCTTTCGAGTGCCGCAACGGCAACCTGGCGGTCTTCTCGCGAGTGTCCAGCGTACGCGAGTGGATCCGACAAGTCACCAACATATAA> M. sexta-Hemolymph proteinase 18 (MsHP18); AY672794.1 (SEQ ID NO: 10)ATGGTTTATATTTTAATAATTTTAGTGATTTGCAATTTTAGTTGTATTAGTTGTCAGTCCGGGACAGTGGAAAGCAGGATTCATTTTAAAGATGAAGGGCCGGAATGTTATGATGCAAATAAAAAGGGCACCTGTGTTAGTGCTCACAGATGCCTTGATGTAGTTAGAAAACTTAAAGACGGAGAGAAACCCACGATATGTGGCTACCAAGGCACGGAACCAATGGTGTGTTGCACAGACTGTACTCTGGTTGATAATATTAGTAATTTGGTCGTAAGTTCCATATCCGGGTACCTGTGGAAGGATGGTCAGAAAGCGTGGGACAAATGTCTGGAATACGTTGACAAGCTGTCGTACCCATGCGCTTCAACCTACTCCCACTACCTCAGCTCCGTTTGGGAGAAAGATAAGGAGTGCAGTATGGTTCAGTTTGTTGGCGTGAGGCGATTCGCCTCGTATAACGGACAACCGGCGAAACGGAACGAGTACCCTCACATGGCTCTGCTCGGCTACGGCGACGACCAGGAGACGGCGCAGTGGCTCTGCGGCGGCTCAGTGATCAGTGATCAATTCATCCTCACGGCTGCACACTGCATCTTTACAAATCTATTGGGTCCAGTACGTTTCGCAGCGCTAGGAATACTGCAGCGATCGGATCCAGTAGAGTTATGGCAAGTTTACAAGATCGGCGGCATAGTTCCCCATCCGCAGTATAAGTCACCTATCAAGTACCACGACATTGCTCTCCTGAAGACTGAAAACAAAATAAAGTTTAATGAGAACGTGCTGCCAGCGTGTTTGTTCATAGAGGGCAGAGTGGGTGGGAGTGAGCAGGCTAAAGCGACCGGTTGGGGCGCGCTTGGACATAAACAGACGGCAGCTGACGTACTGCAAGTGGTTGACCTTCAAAAGTTCAGTGACGAAGAGTGCGGAAGTACCTACCGTCCTTACCGGCATTTGCCTCAAGGCTACGACAGCGCCACGCAGATGTGCTACGGCGACAAGGGAAAACTGAATATGGACACCTGTGAGGGCGACAGCGGCGGTCCTCTACAGTTCCAAAACTCCTCGCTCCTCTGCATACACATAGTAGCGGGAGTGACGTCATTCGGCGACGCGTGCGGGTTTGCGGGCGGCGCCGGGATGTACACACGAGTGTCGTACTATATTCCCTGGATCGAGAGCGTTGTATGGCCGTGA> M. sexta-Transferrin (MsTRN); M62802.1 (SEQ ID NO: 11)ATGGCTTTGAAACTTTTAACTTTGATAGCCCTGACTTGTGCGGCTGCGAATGCAGCTAAATCTTCATACAAACTATGCGTGCCAGCAGCATACATGAAGGACTGCGAGCAGATGCTTGAAGTACCCACGAAGTCTAAAGTGGCCTTGGAATGTGTACCGGCTAGAGACAGGGTGGAATGCCTCAGCTTTGTTCAGCAGCGACAGGCGGACTTCGTCCCCGTCGACCCTGAGGACATGTACGTGGCCTCCAAGATCCCCAACCAGGACTTCGTCGTCTTCCAGGAGTACAGGACTGATGAAGAGCCTGATGCGCCATTCCGTTATGAAGCCGTTATTGTGGTTCACAAAGACCTACCCATCAACAACTTGGATCAGCTGAAGGGACTGAGGTCTTGCCACACCGGAGTCAATCGTAACGTCGGGTACAAGATCCCACTAACGATGTTGATGAAACGTGCCGTGTTCCCGAAAATGAACGACCACAGCATTTCGCCGAAAGAGAACGAACTGAAAGCGCTATCGACGTTCTTCGCAAAGTCGTGCATCGTCGGCAAATGGTCGCCTGACCCCAAAACCAACTCGGCTTGGAAATCACAATACAGCCATTTGTGTTCAATGTGCGAACACCCGGAGCGTTGTGACTATCCCGACAATTACAGCGGGTACGAGGGCGCGTTGAGATGCCTCGCCCACAACAACGGGGAGGTCGCGTTCACCAAAGTCATATTCACACGTAAATTCTTTGGGCTTCCAGTAGGTACCACTCCAGCGAGTCCATCAAACGAAAATCCCGAAGAGTTCAGATATCTCTGCGTGGACGGATCTAAAGCCCCCATCACTGGCAAGGCTTGTTCATGGGCTGCCAGACCTTGGCAAGGACTGATCGGTCACAATGACGTACTTGCCAAACTCGCTCCGCTCAGAGAGAAGGTTAAGCAACTTGCTGATTCTGGTGCAGCTGACAAACCGGAGTGGTTCACCAAAGTCCTTGGTCTATCAGAGAAGATCCACCATGTCGCTGACAATATCCCAATCAAGCCCATCGACTACCTGAACAAGGCTAACTACACGGAGGTCATTGAAAGAGGACATGGAGCTCCCGAGCTGGTCGTCAGGCTATGTGTGACGTCAAACGTGGCATTATCTAAGTGCCGGGCTATGTCCGTGTTCGCATTCAGTAGAGACATCAGGCCGATCCTAGACTGTGTTCAAGAAAACAGCGAAGATGCCTGTCTTAAGAGCGTCCAAGACAACGGTTCAGATCTTGCCTCAGTAGACGATATGAGAGTAGCTGCAGCGGCTAAGAAGTACAACTTACATCCAGTTTTCCACGAAGTGTATGGAGAGCTAAAGACGCCCAACTACGCAGTGGCTGTTGTCAAGAAGGGCACTGCCTACAACAAGATCGACGACTTAAGGGGAAAGAAATCTTGCCACAGCTCTTACAGTACTTTCAGCGGTCTGCACGCGCCTCTCTTCTACCTTATTAACAAGAGGGCCATTCAATCTGACCACTGCGTGAAGAACTTGGGAGAATTCTTCTCAGGCGGATCTTGCTTGCCTGGTGTCGACAAACCCGAAAACAACCCAAGCGGTGATGATGTGTCTAAATTGAAGAAGCAATGTGGATCCGACAGCAGCGCTTGGAAGTGCTTGGAAGAGGACAGAGGAGACGTCGCATTTGTTTCAAGTGCCGATCTGTCCCACTTCGACGCCAACCAATACGAGCTGCTCTGCCTGAACCGCGACGCTGGCGGTAGAGATGTTCTCTCCAGTTTCGCCACTTGCAACGTCGCCATGGCCCCGTCCAGGACCTGGGTGGCTGCGAAGGACTTCCTGTCTGACGTATCTATCGCCCACACACCATTGAGCCTCGCCCAAATGCTCGCTACGAGACCTGACCTCTTCAACATTTACGGAGAGTTCTTGAAGAACAACAATGTTATTTTCAATAATGCCGCTAAAGGCTTAGCAACAACTGAGAAACTTGACTTCGAGAAGTTCAAGACCATCCACGACGTCATCTCTTCATGTGGTCTC GCCTAA> M. sexta-Arylphorin β subunit (MsARP); M28397.1 (SEQ ID NO: 12)ATGAAGACTGTCATAATCCTAGCGGGGTTGGTGGCCCTGGCCCTCGGCAGCGAAGTGCCTGTCAAGCACTCCTTCAAAGTTAAGGATGTTGATGCGGCTTTCGTCGAACGTCAAAAGAAGGTCTTAGATCTTTTCCAAGATGTCGACCAAGTAAATCCTAACGATGAGTACTACAAGATTGGCAAGGAATACAACATCGAGGCTAACATCGACAATTACTCGAACAAGAAGGCCGTCGAAGAATTCTTGCAGTTATACAGGACAGGTTTCTTGCCTAAGTACTATGAATTTTCACCCTTCTATGACAGACTAAGGGACGAGGCCATTGGTGTTTTCCACCTCTTTTACTACGCTAAAGATTTTGATACGTTCTACAAATCTGCCGCATGGGCGCGTGTGTACCTCAACGAAGGACAGTTCTTATACGCCTACTACATTGCTGTGATTCAGCGTAAAGATACTCAGGGCTTCGTTGTACCAGCACCGTATGAAGTCTACCCTCAATTCTTCGCAAACTTGAACACTATGCTCAAAGTCTACCGTACCAAAATGCAGGATGGAGTTGTTAGTGCCGATTTAGCTGCACAACACGGCATCGTAAAGGAGAAAAACTACTACGTATACTATGCCAATTACTCCAACTCATTAGTGTACAACAACGAGGAACAGAGACTGTCGTACTTCACTGAGGACATCGGCTTGAATTCGTACTACTACTACTTCCACTCTCACTTGCCTTTCTGGTGGAATTCTGAGAGATACGGAGCACTAAAATCGCGCCGTGGTGAAATCTACTATTACTTCTATCAGCAATTAATTGCACGTTATTACTTTGAACGTCTCTCGAACGGCCTGGGTGACATTCCCGAATTCTCATGGTACTCACCAGTCAAGTCTGGCTACTATCCACTGATGTCTTCTTATTACTACCCCTTCGCTCAAAGGCCCAACTACTGGAACGTGCACAGCGAAGAAAACTACGAGAAAGTACGATTCTTGGACACGTATGAAATGTCATTCCTTCAGTTCCTCCAAAACGGACACTTCAAAGCGTTTGACCAGAAGATTGACTTCCACGATTTCAAAGCTATCAACTTTGTTGGAAACTACTGGCAAGATAATGCTGACCTGTACGGTGAGGAAGTTACTAAGGACTACCAACGTTCATATGAAATTATAGCCCGCCAAGTGCTTGGTGCTGCACCTAAACCATTCGACAAGTACACATTCATGCCCAGCGCTTTAGACTTCTACCAGACGTCTCTGCGTGACCCAATGTTCTACCAACTTTACAACAGAATTCTGAAGTACATATATGAGTACAAGCAGTACCTGCAACCGTACTCTTCAGAAAAACTGGCATTCAAGGGTGTCAAGGTGGTCGATGTTGTAGTAGACAAACTGGTTACCTTCTTCGAGTACTACGACTTTGATGCGTCCAACAGCGTTTTCTGGAGCAAAGAGGAGGTTAAATCTAGCTACCCCCATGATTTCAAGATCCGTCAGCCACGCCTTAACCACAAGCCATTCTCTGTCTCTATCGACATCAAATCTGAAGCTGCCGTTGATGCCGTTGTCAAGATATTCATGGCACCTAAATACGACGATAATGGATTCCCTCTGAAATTAGAAAACAACTGGAACAAATTCTTCGAGCTGGACTGGTTCACATACAAATTTGTTGCTGGTGACAACAAAATCGTGAGGAACTCAAACGACTTCTTGATCTTCAAGGACGACTCTGTTCCCATGACTGAGTTGTACAAATTATTAGAACAAAATAAGGTTCCACACGACATGTCCGAGGATTACGGCTACCTGCCTAAAAGACTGATGCTGCCAAGAGGTACTGAGGGTGGTTTCCCATTCCAGTTCTTCGTTTTCGTATATCCATTCAACGCTGACAGCAAAGATCTTGCACCGTTCGAGGCCTTCATCCAGGACAACAAACCTTTGGGCTATCCATTCGACCGTCCCGTTGTTGACGCTTACTTCAAGCAACACAACATGTTCTTCAAGGACGTCTTCGTATACCATGACGGCGAGTACTTCCCGTACAAGTTCAATGTTCCTTCCCATGTGATGCACTCAAACGTTGTTCCTAAACACTGA> M. sexta-Chymotrypsinogen-like protein 1 (MsCTL1); AM419170.1(SEQ ID NO: 13)ATGTACGTGAAAGTAGCACTTCTGTTGGTAGCCCTCATTGCTGGGAGCTGGGCCTTCCCAAAGCTCGAAGATGAGCAGGACATGTCCATCTTCTTCACGCAGCTCGATTCGAGCGCGCGTATCGTGGGTGGTACCCAGGCCCCCAGCGGAAGTCACCCTCACATGGTGGCGATGACCACCGGTACCTTCATCAGGAGCTTCAGCTGTGGAGGCTCAGTTGTCGGTAGACGTTCCGTTCTGACTGCGGCTCATTGCATCGCTGCTGTTTTCAGTTTCGGTTCCCTCGCCAGTACCCTCCGCTTGACGGTCGGCACCAACTTCTGGAACCAGGGAGGCACCATGTACACCGTCGCTCGCAACATAACCCACCCCCACTACGTCTCTGCGACCATCAAGAACGACATCGGTCTGTTCATCACTCACAACAACATCATCGACACGACTGTCGTCCGCAGCATCCCTCTTAACTTTGACTATGTGCCCGGTGGTGTTCTCACTAGAGTCGCCGGATGGGGCAGGATCAGGACCGGCGGTGCCATCTCTCCCTCTCTGCTGGAGATCATTGTGCCTACTATCAGTGGAAGCGCATGCGTAGCCAGTGCAATCCAAGCTGGCATCGATCTGAACATGAGACCACCTCCCGTCGAGCCTCACATCGAGCTGTGCACCTTCCACGGTCCTAACGTAGGCACTTGTAATGGTGACTCCGGCAGCGCTCTTGCCCGCCTAGACAACGGCCAGCAGATCGGTGTGGTATCGTGGGGCTTCCCGTGCGCACGCGGCGGTCCCGACATGTTCGTCAGGGTCAGCGCCTACCAATCCTGGCTGCAGCAGAGCATC GTATAA> M. sexta-Valine Rich Midgut Protein (MsVMP1); NCBI accession numbernot assigned as yet (SEQ ID NO: 14)ATCATTGACGGACCTTCCGTTGGACCNGCCATCATCGGCGCTGGAGACATCGCTGTCGGCCCTGCTATCGTCGACTTCCCTTTCCCCGACGGCGGTGCCGTGTCTGCCCCCGTTGAGCCTTCCCCCATCGCCATCGGACCCGCTATCGTCGGTGAATCCCCTATCTCCGTCGGACCTGCCATCGTTGAGGCCGGAGACATCGCTGTTGGACCCGCTATCATCGACTTCCCCCTTCCCGACGGTGGCGCCGTGTCCGCCCCCGTTGAGGTTTCTCCCGTCGACTCCGTCGTCGTCGGCCCTGCCGCCGGCTCTCAGAGCTCTCCCCTCGTCCAGATCATCATCAACGTTAAGGCCCCCGCTGGTGCCGGCCCCGTTGTCGATGCCGTCGCTGACAAGCCCATGGACATCATTGATGTTATGCCCGTCGTCGACCCTGCTGATTTCGTGGACCTCACCCCCGTTGTAGAGCCTGTAGAAGTCGTCGACATTGTCGATGTCATGCCCGTGGTTGACCCCATCAACATCATCGATGTTATGCCTGTTGTTAAGCCCGTAAACCCCCTTGCCCGTTCTTAAGGG> M. esculanta-Catalase 1 (MeCAT1); AF170272 (SEQ ID NO: 15)ATGGATCCTTGCAAGTTCCGTCCATCAAGCTCAAACAATACCCCCTTCTGGACCACCGATGCTGGTGCTCCAGTATGGAACAACAATTCCTCCATGACTGTTGGAACCAGAGGTCCAATCCTTTTGGAGGACTATCATATGATAGAGAAACTTGCCAACTTTACCAGAGAGAGGATTCCAGAGCGTGTCGTCCATGCTAGGGGAATGAGTGCAAAGGGCTTCTTTGAAGTCACCCACGATGTCTCTCACCTTACTTGTGCTGATTTCCTTCGAGCCCCTGGAGTTCAAACCCCTGTCATCGTCCGTTTCTCCACTGTTATCCACGAGCGTGGCAGCCCTGAAACACTCAGGGATCCTCGAGGTTTTGCGACTAAGTTCTACACCAGAGAGGGCAACTTTGATATTGTGGGAAACAACTTCCCTGTCTTCTTCATCCGTGATGGAATAAAATTCCCAGATGTGATACACGCTTTTAAGCCCAATCCCAAGTCTCACATCCAAGAATACTGGAGGATCTTTGACTTCTTATCACACCATCCTGAGAGCTTGAGCACCTTCGCCTGGTTCTTCGATGATGTTGGAATTCCCCAAGATTACAGACACATGGAAGGTTTCGGTGTTCACACCTTTACTTTCATCAACAAGGCTGGAAAAGTAACCTACGTGAAATTTCACTGGAAACCCACTTGCGGGGTCAAGTGTTTGATGGATGATGAGGCACTTAAGATCGGAGGTGCCAACCACAGCCATGCTACGCAGGATTTATACGACTCCATTGCCGCTGGCAACTATCCTGAGTGGAGACTCTTCATCCAGACAATGGATCCAGCTGATGAAGACAAATTCGACTTTGATCCACTTGATATGACCAAGATCTGGCCTGAGGATATTTTTCCTCTACAGCAAATTGGCCGTTTGGTCTTGAACAGGAACATCGATAACTGGTTTGCTGAGAATGAAATGCTCGCATTCGACCCTGGTCATATTGTTCCTGGCATTCACTATTCAAACGACAAGTTGTTTCAGCTCAGAACCTTTGCATATGCTGACACTCAGAGGCACCGTCTCGGACCCAACTATAAGATGCTCCCTGTTAATGCTCCCAAGTGTGCTTATCACAACAATCATTACGATGGTTTCATGAATTTCATGCACAGGGATGAGGAGGTGGATTACTTCCCATCCAGGTATGATCCAGTTCGCCATGCTGAGAGAAGCCCCATTCCTAACGCTATCTGTAGTGGAAGGCGTGAAAAGTGCGTCATTGAAAAGGAGAACAATTTCAAGCAACCTGGAGAGAGATATCGATCCTGGGCACCTGATAGACAAGAAAGATTCCTGTGCAGATTGGTTAACGCCTTATCAGAGCCACGTATCACCTTTGAGATTCGCAGTATCTGGGTCTCTTACTGGTCTAAGTGCGACGCGTCTCTGGGTCAAAAGCTGGCTTCTCGTCTCAACGTGAGGCCAAATATATGA> M. sexta-Hemolin (MsHEM); M64346.1 (SEQ ID NO: 16)GGCAAAGAATTCAAATGGCAGGAACACAATATCGCCCAGCGCAAAGACGAAGGCAGCCTGGTCTTCCTCAAGCCCGAGGCTAAAGATGAAGGCCAATACAGATGTTTCGCTGAGTCGGCCGCCGGAGTCGCCACCTCCCACATCATCTCCTTTAGAAGGACCTACATGGTCGTACCTACTACTTTTAAGACTGTAGAAAAGAAACCGGTAGAAGGGTCATGGCTCAAACTTGAGTGCAGCATCCCCGAAGGTTATCCTAAACCTACTATTGTATGGAGAAAGCAGCTTGGTGAAGACGAAAGTATAGCAGATTCTATACTGGCACGTCGTATTACACAATCTCCAGAGGGAGACCTGTACTTCACGAGCGTCGAGAAAGAAGACGTAAGCGAAAGCTATAAATACGTTTGCGCTGCTAAGTCACCGGCTATTGATGGGGATGTCCCTCTTGTTGGATACACTATTAAAAGCTTAGAAAAGAATACAAATCAGAAAAACGGTGAGCTGGTCCCGATGTACGTCAGTAATGATATGATAGCTAAGGCCGGAGACGTTACTATGATCTACTGCATGTATGGTGGAGTCCCAATGGCTTACCCC AACTGGTTCAA> M. sexta- Serine Proteinase homolog 3 (MsSPH-3); AF413067.1(SEQ ID NO: 17)ATGTTGTTGCTTCTGTATTGTCTTGTGGCGGCCTCCGCGCCGTTCTTTATTGCAGCGGACCAAGGCAGCCCTGACCTGCCTTTAGCTACCGAACCACCAACAGAATGCGGAACAATAGCACCTGATGATAGCTTAGTATTAGATGGGTCCGTTGGTAAAAGTGACAAATTACCTTGGTATGCTATTATCTACACAACCACCACCCGGCCATACAAGCAGATCGGTGGAGGAACCCTCATCACTCCTTCAGTAGTAATCTCAGCCGCTCACTGTTTCTGGCGCAATGGTGAGGTTCCATCTAAGGATAATTACGCCGTGGCGCTCGGCAAGACCCATAGTGCTTGGAATAGCCATGCCGATGTAAACGCTCACAAGTCTGATGTAAAAGAAATACACATACCACCGCAGTTTAAGGGAAGGAACACTAATTATCGGAATGATATAGCAATCGTGGTCATGTCAGACCCTGTGACCTACAAAGTGGACATCCGCCCTATCTGTTTGAACTTCGATGTACAATTTGAAAGACTGCAATTAAAAGACGGCATTATGGGGAAGATCGGCACATGGAATGTAAGTCGTGAGACACTGAAACTATCGAAAACATTAAAAGTGGTGGAGAATCCATACATTGACGCAGCGACT> M. sexta- Peptidoglycan recognition protein 2 (MsPGRP2); GQ293365.1(SEQ ID NO: 18)ATGGCGAGCTTCGCTTTAATAGTTATCCTTAGCGTAATTGGCTTTATATCGGCCTATCCTAGTCCTGAAGGTTACAGTTCTGCCTTCAACTTTCCATTCGTAACCAAGGAGCAGTGGGGCGGCAGGGAGGCACGCACGTCGACGCCACTCAACCACCCAGTGCAGTTCGTGGTGATCCACCACAGTTACATTCCCGGCGTGTGCCTCAGCCGGGACGAGTGCGCGCGCAGCATGCGCTCCATGCAGAACTTCCACATGAACAGTAACGGGTGGAGTGATATTGGATACAACTTCGCTGTCGGCGGTGAAGGGTCGGTGTACGAGGGCCGCGGCTGGGACGCGGTCGGCGCACACGCAGCTGGCTATAACAGTAACAGTATCGGCATCGTGCTCATCGGCGATTTTGTTTCAAACCTCCCGCCGGCGGTGCAAATGCAAACCACACAAGAATTGATCGCAGCGGGCGTGCGACTCGGTTACATCAGGCCCAACTACATGCTCATCGGGCATCGTCAGGTCTCCGCCACTGAGTGCCCAGGAACCAGACTCTTCAACGAAATCACCAACTGGAACAACTTCGTGAG> M. sexta- Beta-1, 3-glucan-recognition protein 2 (Ms8GRP2); AY135522.1(SEQ ID NO: 19)GCGTCTGTTTGTTCGCAACCATTGCGGGCTGCTTGGGCCAGCGAGGGGGTCCATACAAGGTGCCTGATGCGAAACTCGAAGCTATCTACCCCAAAGGCTTGAGAGTCTCTGTGCCAGATGATGGCTACTCCCTATTTGCCTTCCACGGCAAGCTCAATGAGGAGATGGAAGGTTTAGAGGCTGGCCATTGGTCCAGAGACATCACCAAAGCGAAGCAGGGCAGATGGATATTCAGAGATAGGAATGCTGAGCTGAAGCTTGGAGACAAAATTTACTTCTGGACTTACGTTATTAAGGATGGATTGGGATACAGGCAGGACAATGGAGAATGGACTGTTACAGAATTCGTCAATGAGAACGGTACAGTGGTGGACACTAGTACAGCGCCGCCACCAGTAGCACCCGCCGTTTCAGAGGAAGATCAATCGCCAGGTCCTCAGTGGAGACCTTGCGAAAGATCCCTGACTGAGTCCTTGGCCCGCGAACGCGTTTGCAAAGGCAGCCTTGTCTTTAGCGAGGACTTTGATGGTTCCAGTTTGGCCGACTTGGGCAATTGGACCGCTGAAGTCAGATTCCCTGGCGAACCGGACTACCCGTACAACTTGTACACTACGGACGGCACTGTGGGATTCGAAAGTGGGTCTCTGGTGGTGAGACCCGTCATGACCGAGTCCAAATACCACGAGGGCATCATATACGACCGCCTCGACCTTGAGAGATGTACAGGACAGCTGGGTACGCTGGAATGCAGGCGAGAGAGCAGCGGCGGTCAGATTGTACCACCTGTGATGACAGCTAAACTGGCCACTCGACGCAGCTTCGCGTTCAAGTTCGGCAGGATCGATATAAAGGCGAAGATGCCGCGCGGGGACTGGTTGATACCAGAACTCAACCTCGAACCTTTAGATAACATATACGGCAACCAGCGATACGCTTCGGGTCTCATGCGGGTCGCGTTCGTGAGAGGAAACGATGTATACGCCAAGAAGCTCTACGGAGGTCCGATAATGTCCGACGCGGACCCGTTCAGGTCCATGCTGTTGAAGGACAAGCAAGGGTTGGCCAACTGGAATAATGATTACCACGTCTACTCGCTGCTGTGGAAGCCTAACGGTTTAGAGCTGATGGTGGACGGTGAAGTGTACGGCACCATCGACGCTGGCGATGGCTTCTACCAGATTGCGAAGAACAACCTCGTGAGCCACGCCTCGCAGTGGCTCAAGGGCACCGTCATGGCGCCGTTTGATGAAAAGTTCTTCATCACTCTGGGTCTTCGCGTGGCGGGTATCCACGACTTCACGGACGGTCCGGGCAAACCTTGGGAGAACAAGGGC > M. sexta-Relish family protein 2A (MsREL2A); HM363513.1(SEQ ID NO: 20)AGTGTGCGTCTACGAATGATTTGAAAATATGCCGCATAAGCCGTTGTTACGGTAGACCGAGAGGCGGCGAAGATATCTTCATATTTGTCGAAAAGGTCAACAAGAAAAACATCCAAGTTCGGTTCTTTAGACTGGAAAACGGGGAGCGCACCTGGTCAGCGATGGCGAACTTTCTGCTAAGCGATGTTCACCACCAATACGCTATCGCTTTTAGAACGCCACCGTACGTCAATCACCAAATTTCTGAAGACGTGCAAGTTTTTATAGAACTCGTACGCCCTTCAGACGGTAGGACGAGCGCTCCCATGGAGTTCACATACAAGGCTGAGCAAATCTATAAACAGAACAAGAAACGTAAAACTACTTCGTCGTACTCGTCGCTCGACAGCTCCTCAGGTTCGGCCGGTTCAATTAAAAGCATCAGCGAACTGCCCGCGCCCGTTGTTTTTGCTGAAAACGTAAGTTTTTTCTATGACACATTACTCATTCTTCAACCCATGACGAATCTATAA > M. sexta-Dorsal (MsDor); HM363515.1(SEQ ID NO: 21)ATGCTTGTGACGTTATGCGGCGGGAACTATAGTGGATTGTCGTTAACAAAAACTAATCATTATATGTCACCAAAATCATATGTGCCAGGAAATGGTTATGACGCCGCCGTAATCCTAGGTACCACGGAGCAGAATGACAGCGAACCCTCAAACTTGAATATTAGTGATGTTTTTGAAGCCATCACGCTCGCTGATCCGTCGTTCGGCGCGGGCGTGCCGTCGGTAGAGGAGACGATGGCGCACACGCAGCCCCAGCCGCTGCAAATGCCGTACGTGGTCGTCGTGCAGCAGCCCGCCAGCAAAGCGCTCAGATTTCGATATGAGTGCGAGGGCAGATCAGCCGGTTCGATTCCCGGCGCGTCGAGCACGCCCGAGAACCGAACCTTCCCCGCCATCAAGATAATCGGCTACACCGGCACCGTCTCCATCGTAGTGTCGTGTGTCACCAAAGATGAGCCTTGCAGGCCGCACCCACACAACCTGGTCGGGCGCGACCACTGCGACCGCGGCGTGTTCTCCGTCCGCATCGAGATCACCGACGAGAATAACGAATACCAGTTTCGGAACCTGGGCATACAGTGCGTCAAGCGGCGCGACATCGGCGAGGCGCTGCGGATCCGAGAGGACCTGCGCGTCGATCCGTTCAAAACCGGCTTCACCCACCGGAACCACCCGCAAGGCATCGATCTGAATGCAGTGCGGCTCGCGTTCCAAGTGTTCCTGCCGCACTCCAGCGGCAAGATGCGGCGCACGCTCGCGCCCGTCGTGTCCGACGTCATC TACGACAAG> M. sexta-Spätzle (MsSPZ1A); GQ249944.1 (SEQ ID NO: 22)CGAACAACCTGACAGACGGATAGCGGGACGGTCAGCACAATACGAACATTTAAGAACAAACGAGAGGTCTCTCCCGGTCTACAGCGAGACCCAGAGGATACAAGCAGAAGAGAGAAGAAGACACAGTTCGAGACTAGAAGAACCGAGACAACGTGCTGAGAATGGTTCATATAAGATATTGAATAACCCTCCGAAACCCTGTATTACTAATAGGAGAAGTCAAATTGATTCGTCGAATGATAGGGTAGTGTTCCCCGGTCCGACTTCAGAAAGGTCGTACGTACCCGAAGTGCCAGAGGAATGCAAGAAAATCGGCATATGCGACAGTATACCGAATTACCCAGAAGAACACGTAGCTAATATTATATCTCGACTTGGAGACAAAGGAAAAGTATTACAAATAGACGAACTGGACGTATCAGACACTCCAGATATCGCCCAGAGGTTGGGTCCGCAGGAGGACAACATGGAACTATGTAGCTTTAGAGAAAAGATTTTTTACCCCAAGGCAGCGCCAGACAAAGATGGAAATTGGTTCTTCGTTGTGAATTCAAAAGAAAACCCAGTACAGGGTTATAAAGTTGAAATTTGCGACCGTCAGCAATTACCATGCGCGGAGTTCGCGAGCTTCCAACAGGGATATGAAGCGAGGTGCATCCAGAAATACGTTCGCCGGACCATGTTGGCGTTGGATCCCAAGGGTCAGATGACCGACATGCCCCTTAAAGTGCCCAGCTGTTGCT > M. sexta-Toll receptor (MsTOLL); EF442782.1(SEQ ID NO: 23)CAAGTGGTCGTTGTGCACTGCACCGGACTCGCCGAGTTCCCGCGCCTGCCACTAACCACGGACAAGTGGATCCTGCACCTGCCCCACAACAACATATCATACCTCGCCGCGGCCGACGTATCGCCGAACATCGTGGAACTCGATCTAAGAAACAATTCAATCAAGAATATCGACGTACAGGCATCAGCAAAACTAGCCTTCGTCCGGCTGCAATTGGGTGGTAACCCGATCGAGTGTGACTGCGAGGCGTTGAAGCTGCTGGCGCCGCTGCTCAAACCTGACTCAAAGCTGCTTGACAGAAAGGACGTGAAATGTGAGAACGACGCGCAGATTACCTTGGCGATGCTGAAATTATGTACTAAGTCATCCAATGGGCTGATGTACTTGCTGTTCCTGTTGCTGTTACTCGCATTTGTCGTAACCGGACTGCTCGCACGAACCGCAATTCGCCTGCGCATCAAAATGATCCTCATGAGACTGGGTTGGATGTCGAGACTACTGGAGCCCGCGGACGACGATCGCCCGTACGACGCGTTTGTGTCTTTCGCACACGAGGATGAGGAGCTGGTGATGGAGCAGCTGGCGGCACGGCTCGAGAGCGGCTCGCGGCCGTACCGACTGTGTCTGCACTACCGCGATTGGGCGCCAGGCGAGTGGATCCCGGCGCAAATAGCGGCTTCGGTGCGGGCCTCTCGGCGCACGGTGGCGGTTGTGTCGGCGCACTACTTACAGTCGGGCTGGGCGCTTGCCGAGATCCGGGAGGCGACCGCAGCCTCGCTGCAGGAAGGCATGCCACGTCTCATCATCGTGCTGCTCGACGAGACCGACCGGTTGATGCTCGATATAGACCCTGAGTTGCACGCCTATGTGCGCAACAATACCTACGTGCG> M. sexta-Scolexin A (MsSCA1); AF087004.1 (SEQ ID NO: 24)TCGAAGCTAAGGGGGCAAAGCAGCCAATTGATACGAGGGCAGTGAAGGAACGGTATCCATACGCAGTTCGGAGTTTCGGAGGCTTCTGCGGAGGAACCATTATCAGTCCCACCTGGATCCTGACCGCCGGCCACTGCTCGATACTCTATGCGGGGAGCGGCCTACCGGCCGGCACCAACATTACCGAGGTATCTAGCTTGTACCGCTTCCCCAAGCGGCTCGTCATACACCCGCTCTTCTCCATAGGACCCGTCTGGCTCAACGCTACGGAGTTCAACCTCAAACAGGCGGCTGCACGATGGGACTTCTTGTTGATAGAACTGGAGGAACCGCTGCCGTTGGACGGCAAGATCCTGGCGGCTGCGAAGCTCGACGACCAGCCCGACCTCCCCGCAGGCCTCGACGTGGGCTATCCGAGCTACAGCACCGACACCTACGAGGCTAAGATACAAAGCGAGATGCACGGAAAGAAGCTTTCGGTTCAATCTAACGAGGTGTGCTCGAAGCTAGAGCAGTTCAAGGCGGAGGACATGTTGTGCGCCAAGGGACGTCCACCGCGATACGACTTCGTCTGCTTCAGCGACAGTGGCAGTGGGCTAGTAGACAACAATGGTCGCCTAGTCGGCGTGGTGTCGTGGGCCGAGAACAACGCTTTCGAGTGCCGCAACGGCAACCTGGCGGTCTTCTCGCGAGTGTCCAGCGTACGCGAGTGGATCCGAC AAGTC> M. sexta-Hemolymph proteinase 18 (MsHP18); AY672794.1 (SEQ ID NO: 25)GATGGTCAGAAAGCGTGGGACAAATGTCTGGAATACGTTGACAAGCTGTCGTACCCATGCGCTTCAACCTACTCCCACTACCTCAGCTCCGTTTGGGAGAAAGATAAGGAGTGCAGTATGGTTCAGTTTGTTGGCGTGAGGCGATTCGCCTCGTATAACGGACAACCGGCGAAACGGAACGAGTACCCTCACATGGCTCTGCTCGGCTACGGCGACGACCAGGAGACGGCGCAGTGGCTCTGCGGCGGCTCAGTGATCAGTGATCAATTCATCCTCACGGCTGCACACTGCATCTTTACAAATCTATTGGGTCCAGTACGTTTCGCAGCGCTAGGAATACTGCAGCGATCGGATCCAGTAGAGTTATGGCAAGTTTACAAGATCGGCGGCATAGTTCCCCATCCGCAGTATAAGTCACCTATCAAGTACCACGACATTGCTCTCCTGAAGACTGAAAACAAAATAAAGTTTAATGAGAACGTGCTGCCAGCGTGTTTGTTCATAGAGGGCAGAGTGGGTGGGAGTGAGCAGGCTAAAGCGACCGGTTGGGGCGCGCTTGGACATAAACAGACGGCAGCTGACGTACTGCAAGTGGTTGACCTTCAAAAGTTCAGTGACGAAGAGTGCGGAAGTACCTACCGTCCTTACCGGCATTTGCCTCAAGGCTACGACAGCGCCACGCAGATGTGCTACGGCGACAAGGGAAAACTGAATATGGACACCTGTGAGGGCGACAGCGGCGGTCCTCTACAGTTCCAAAACTCCTCGCTCCTCTGC> M. sexta-Transferrin (MsTRN); M62802.1 (SEQ ID NO: 26)ATGGCTTTGAAACTTTTAACTTTGATAGCCCTGACTTGTGCGGCTGCGAATGCAGCTAAATCTTCATACAAACTATGCGTGCCAGCAGCATACATGAAGGACTGCGAGCAGATGCTTGAAGTACCCACGAAGTCTAAAGTGGCCTTGGAATGTGTACCGGCTAGAGACAGGGTGGAATGCCTCAGCTTTGTTCAGCAGCGACAGGCGGACTTCGTCCCCGTCGACCCTGAGGACATGTACGTGGCCTCCAAGATCCCCAACCAGGACTTCGTCGTCTTCCAGGAGTACAGGACTGATGAAGAGCCTGATGCGCCATTCCGTTATGAAGCCGTTATTGTGGTTCACAAAGACCTACCCATCAACAACTTGGATCAGCTGAAGGGACTGAGGTCTTGCCACACCGGAGTCAATCGTAACGTCGGGTACAAGATCCCACTAACGATGTTGATGAAACGTGCCGTGTTCCCGAAAATGAACGACCACAGCATTTCGCCGAAAGAGAACGAACTGAAAGCGCTATCGACGTTCTTCGCAAAGTCGTGCATCGTCGGCAAATGGTCGCCTGACCCCAAAACCAACTCGGCTTGGAAATCACAATACAGCCATTTGTGTTCAATGTGCGAACACCCGGAGCGTTGTGACTATCCCGACAATTACAGCGGGTACGAGGGCGCGTTGAGATGCCTCGCCCACAACAACGGGGAGGTCGCGTTCACCAAAGTCATATTCACACGTAAATTCTTTGGGCTTCCAGTAGGTACCACTCCAGCGAGTCCATCAAACGAAAATCCCGAAGAGTTCAGATATCTCTGCGTGGACGGATCTAAAGCCCCCATCACTGGCAAGGCTTGTTCATGGGCTGCCAGACCTTGGCAAGGACTGATCGGTCACAATGACGTACTTGCCAAACTCGCTCCGCTCAGAGAGAAGGTTAAGCAACTTGCTGATTCTGGTGCAGCTGACAAACCGGAGTGGTTCACCAAAGTCCTTGGTCTATCAGAGAAGATCCACCATGTCGCTGACAATATCCCAATCAAGCCCATCGACTACCTGAACAAGGCTAACTACACGGAGGTCATTGAAAGAGGACATGGAGCTCCCGAGCTGGTCGTCAGGCTATGTGTGACGTCAAACGTGGCATTATCTAAGTGCCGGGCTATGTCCGTGTTCGCATTCAGTAGAGACATCAGGCCGATCCTAGACTGTGTTCAAGAAAACAGCGAAGATGCCTGTCTTAAGAGCGTCCAAGACAACGGTTCAGATCTTGCCTCAGTAGACGATATGAGAGTAGCTGCAGC> M. sexta-Arylphorin β subunit (MsARP); M28397.1 (SEQ ID NO: 27)CTGTCATAATCCTAGCGGGGTTGGTGGCCCTGGCCCTCGGCAGCGAAGTGCCTGTCAAGCACTCCTTCAAAGTTAAGGATGTTGATGCGGCTTTCGTCGAACGTCAAAAGAAGGTCTTAGATCTTTTCCAAGATGTCGACCAAGTAAATCCTAACGATGAGTACTACAAGATTGGCAAGGAATACAACATCGAGGCTAACATCGACAATTACTCGAACAAGAAGGCCGTCGAAGAATTCTTGCAGTTATACAGGACAGGTTTCTTGCCTAAGTACTATGAATTTTCACCCTTCTATGACAGACTAAGGGACGAGGCCATTGGTGTTTTCCACCTCTTTTACTACGCTAAAGATTTTGATACGTTCTACAAATCTGCCGCATGGGCGCGTGTGTACCTCAACGAAGGACAGTTCTTATACGCCTACTACATTGCTGTGATTCAGCGTAAAGATACTCAGGGCTTCGTTGTACCAGCACCGTATGAAGTCTACCCTCAATTCTTCGCAAACTTGAACACTATGCTCAAAGTCTACCGTACCAAAATGCAGGATGGAGTTGTTAGTGCCGATTTAGCTGCACAACACGGCATCGTAAAGGAGAAAAACTACTACGTATACTATGCCAATTACTCCAACTCATTAGTGTACAACAACGAGGAACAGAGACTGTCGTACTTCACTGAGGACATCGGCTTGAATTCGTACTACTACTACTTCCACTCTCACTTGCCTTTCTGGTGGAATTCTGAGAGATACGGAGCACTAAAATCGCGCCGTGGTGAAATCTACTATTACTTCTATCAGCAATTAATTGCACGTTATTACTTTGAACGTCTCTCGAACGGCCTGGGTGACATTCCCGAATTCTCATGGTACTCACCAGTCAAGTCTGGCTACTATCCACTGATGTCTTCTTATTACTACCCCTTCGCTCAAAGGCCCAACTACTGGAACGTGCACAGCGAAGAAAACTACGAGAAAGTACGATTCTTGGACACGTATGAAATGTCATTCCTTCAGTTCCTCCAAAACGGACACTTCAAAGCGTTTGACCAGAAGATTGACTTCCACGATTTCAAAGCTATCAACTTTGTTGGAAACTACTGGCAAGATAATGCTGACCTGTACGGTGAGGAAGTTACTAAGGACTACCAACGTTCATATGAAATTATAGCCCGCCAAGTGCTTGGTGCTGCACCTAAACCATTCGACAAGTACACATTCATGCCCAGCGCTTTAGACTTCTACCAGACGTCTCTGCGTGACCCAATGTTCTACCAACTTTACAACAGAATTCTGAAGTACATATATGAGTACAAGCAGTACCTGCAACCGTACTCTTCAGAAAAACTGGCATTCAAGGGTGTCAAGG> M. sexta-Chymotrypsinogen-like protein 1 (MsCTL1); AM419170.1(SEQ ID NO: 28)ATGTACGTGAAAGTAGCACTTCTGTTGGTAGCCCTCATTGCTGGGAGCTGGGCCTTCCCAAAGCTCGAAGATGAGCAGGACATGTCCATCTTCTTCACGCAGCTCGATTCGAGCGCGCGTATCGTGGGTGGTACCCAGGCCCCCAGCGGAAGTCACCCTCACATGGTGGCGATGACCACCGGTACCTTCATCAGGAGCTTCAGCTGTGGAGGCTCAGTTGTCGGTAGACGTTCCGTTCTGACTGCGGCTCATTGCATCGCTGCTGTTTTCAGTTTCGGTTCCCTCGCCAGTACCCTCCGCTTGACGGTCGGCACCAACTTCTGGAACCAGGGAGGCACCATGTACACCGTCGCTCGCAACATAACCCACCCCCACTACGTCTCTGCGACCATCAAGAACGACATCGGTCTGTTCATCACTCACAACAACATCATCGACACGACTGTCGTCCGCAGCATCCCTCTTAACTTTGACTATGTGCCCGGTGGTGTTCTCACTAGAGTCGCCGGATGGGGCAGGATCAGGACCGGCGGTGCCATCTCTCCCTCTCTGCTGGAGATCATTGTGCCTACTATCAGTGGAAGCGCATGCGTAGCCAGTGCAATCCAAGCTGGCATCGATCTGAACATGAGACCACCTCCCGTCGAGCCTCACATCGAGCTGTGCACCTTCCACGGTCCTAACGTAGGCACTTGTAATGGTGACTCCGGCAGCGCTCTTGCCCGCCTAGACAACGGCCAGCAGATCGGTGTGGTATCGTGGGGCTTCCCGTGCGCACGCGGCGGTCCCGACATGTTCGTCAGGGTCAGCGCCTACCAATCCTGGCTGCAG> M. sexta- Valine Rich Midgut Protein (MsVMP1); NCBI accession numbernot assigned as yet (SEQ ID NO: 29)ATCATTGACGGACCTTCCGTTGGACCNGCCATCATCGGCGCTGGAGACATCGCTGTCGGCCCTGCTATCGTCGACTTCCCTTTCCCCGACGGCGGTGCCGTGTCTGCCCCCGTTGAGCCTTCCCCCATCGCCATCGGACCCGCTATCGTCGGTGAATCCCCTATCTCCGTCGGACCTGCCATCGTTGAGGCCGGAGACATCGCTGTTGGACCCGCTATCATCGACTTCCCCCTTCCCGACGGTGGCGCCGTGTCCGCCCCCGTTGAGGTTTCTCCCGTCGACTCCGTCGTCGTCGGCCCTGCCGCCGGCTCTCAGAGCTCTCCCCTCGTCCAGATCATCATCAACGTTAAGGCCCCCGCTGGTGCCGGCCCCGTTGTCGATGCCGTCGCTGACAAGCCCATGGACATCATTGATGTTATGCCCGTCGTCGACCCTGCTGATTTCGTGGACCTCACCCCCGTTGTAGAGCCTGTAGAAGTCGTCGACATTGTCGATGTCATGCCCGTGGTTGACCCCATCAACATCATCGATGTTATGCCTGTTGTTAAGCCCGTAAACCCCCTTGCCCGTT> M. esculanta- Catalase 1 (MsCAT1) AF170272 (SEQ ID NO: 30)ATGGATCCTTGCAAGTTCCGTCCATCAAGCTCAAACAATACCCCCTTCTGGACCACCGATGCTGGTGCTCCAGTATGGAACAACAATTCCTCCATGACTGTTGGAACCAGAGGTCCAATCCTTTTGGAGGACTATCATATGATAGAGAAACTTGCCAACTTTACCAGAGAGAGGATTCCAGAGCGTGTCGTCCATGCTAGGGGAATGAGTGCAAAGGGCTTCTTTGAAGTCACCCACGATGTCTCTCACCTTACTTGTGCTGATTTCCTTCGAGCCCCTGGAGTTCAAACCCCTGTCATCGTCCGTTTCTCCACTGTTATCCACGAGCGTGGCAGCCCTGAAACACTCAGGGATCCTCGAGGTTTTGCGACTAAGTTCTACACCAGAGAGGGCAACTTTGATATTGTGGGAAACAACTTCCCTGTCTTCTTCATCCGTGATGGAATAAAATTCCCAGATGTGATACACGCTTTTAAGCCCAATCCCAAGTCTCACATCCAAGAATACTGGAGGATCTTTGACTTCTTATCACACCATCCTGAGAGCTTGAGCACCTTCGCCTGGTTCTTCGATGATGTTGGAATTCCCCAAGATTACAGACACATGGAAGGTTTCGGTGTTCACACCTTTACTTTCATCAACAAGGCTGGAAAAGTAACCTACGTGAAATTTCACTGGAAACCCACTTGCGGGGTCAAGTGTTTGATGGA> M. sexta-IMD (MsIMD); Msex2.05477-RA (SEQ ID NO: 31)ATGACTTCTTTGAAAAGCAAATTAGCAGAATTCTTGAAGGGGTTAAAATCAGATGCAACCCCAAGCCCCGAGGCCATCGACAGAACCCAGGGTAAATCCACAAACTGAGCAATGAAAATGACGCACCCTCGGATAGTGAACCTGAAGAAATCATTATAGAAGATATGACGATACGAAGAAAAAGAAAAAGGCAAGTTACAAGAAACCGTTCTTTAGCTCCAAACCTGGTGCATTCCCCGAAAAGAAAAAAGACAAGAATACCAAAGACGATTACAGCAACTTTGTGAATACTCAAGCCACTGGTGATGTCATCAATATTGTAGGCTGTAACAATTTCCGCTGGGGTAATAATTATTATTTGGGAAATACCAAGAAACAGGCTCCTCCTAAGAAATATTTCCAAGAAGAGGAAGACAGTGAACCAGAAGATGATATTCAGAAATGCAACTTAATCAAACTGCTATTTGAAGCTGAAAATAAGCCAGAACATGAGTACCTGGACTACATTTCCCAGAACATGAATGAAAAGTGGCACAGATTCTTTGTGAAACTAGGTTTTACACCAGGAAGGATCAAAACATCCATCATAGATAATGCCAGTTATGGTATTTCAGAGGCTCGATACGCATTGCTACTTGAGTGGGTGAATAAAAAACGGGACTCTAATCTTGGACAGCTATCGAATTTATTGTGGAAACACGGCGAGAGGCGAATTGTCAAAGAATTAGCCATTATGTACTCTGCAAGCAAGGCCAAATCTGATGATGAATAG> M. sexta-FADD (MsFADD); Msex2.03129-RB (SEQ ID NO: 32)ATGACTCTATCGGAATCAAAATTTAAACAATTAAAAGAACAAATTATTTTACATGCAAGTGCAACTGAAAGACATGCTCAAATTTTGAACTCATTAAAGGATTTGTTTAAAGAAGATATTAATTCTGTTCGAAGATTTGAACAAATCAGCAATATAGCACAATTATTAAAAGTTTTAGAAATAAGAGATGTGTTGTCAGAGGATGATGTTGCTCCTTTGAAAGATGTGGCACGCCAACTACCAAATAGCTCAGAAATGCTGCGGAAAATTGCTGAATATGAAGAAAATCACAAGTGTGGAGAGTTTATTTCTGTTCCTAAGTCACCTCCAAAACAAAAAGAACATAGTCATTCTGGATGGGATATTAACAGCATACAGAATTCTGAATATTCTGTAAAAAAGGAAAGGATATTTGAAGTTATTTCTGAAGAAATTGGCACCCATTGGAGAAATCTAGCGAGATATTTGAAAACAAGGGAATGTACAATAATTGAAATTGACAGTAAAAACATACCACTTTCAAGTAAAGCTATGGAGATTTTGAAATTGCATTGTAAAAAAGCAAATCCACAAAAGTGGTTTTTTGACTTGTGTAATGCATTGGACAAAGTGCATAGGACTGACATAGTGTTATCACTGCAAGAAATTATGTCTATGAAT ATTTAG> M. sexta-Dredd (MsDRD); Msex2.04297-RC (SEQ ID NO: 33)ATGTTGTCTTCCGATGCCAGAAGTTCCCTAAATTCCAAGAGTGATAATGAAACGTTTATATTAAACTTGGATTCGATTTCAAAGATTGAAAAACAATTACAAGATAATCCTTATGATATGATCTCTTTAGTGTTCCTGTTGTACGACGTGCCGGATACGGCGCTGCAGAGATTGATGGTGTATCAACGAGTGACGAGTGACGTCAGTGGAACAAATATTAATTTGTTGCAAGAATGGTACTGTCACGCCAGCAGTCGCCCTGACTGGCAACATGAATTATTAGAAGCATTAATGATATGCCAATTGAACTCCATTGTGAAGAGCCTTGGTTTCCATATACCCACTATGAGAGTATTTTACCAATCAAATGATCCCTTCAGCAGCAAGTACATAAATCCCGTAAAGAAAGTCCTTTATCATGCCTGTGAAAATATAAACTCAACTAATCTTTTGAAATTAAAAAAATCACTTCTTTCATATGATATAAATGTAATGGAATATACAACTTGTGAGCTTATATTTTTAGAGCTCCTGTCCAATAAATTTATTACTATTAATAATATTGGCCAAAAGATTAAAACAAACCAGAACTGCATATGTAATGTAGAAAACCTTGTGAAGATCTTAGACAACTTAAATGGATTGAAAAAAGTGGCTATGAATTTGAGATATTTTCAAAGTAAATTCAATGATGAAGAGGTGGATTCATTCGCGTCTGTCAATGGAAGCAGCTCACCATCACTTCCAGTGCCTCCTCTTGATGGTACAAAATTAAAGCAAGATGACAAGAATTATGGAGCTGAAGATTTCTCAGAGCTATTCGACATAATAAATAATATGCCAGAACCGCTTGAAAACAATTTTAAATCTGATACAATGTCAACTAAACAGAATAGATATGAGATAAAAAATCCGGAACAACTAGGTGTTTGCATAGTTATAAACCAAGAGAATTTTTATCCATCCAAAAATAGTATTGAAGACCACCAAATTGTTCCATTAGAAGAGAGAAAAGGATCTAGTGTGGATAAAAGGACTTTGGAAAGAACCATGACATCATTAAAATTTCAAGTTCACAGTTGCTCTGATTTAGATCATGATGAAATGATAGAATTCATCAAAAAGAAAATTAATAAACATGTTACATCAAATGACAGTATTTTTATGTTGTGTATACTGTCACATGGTGTAAGGGACCATGTGTATGCTGCTGACTCTGTGAAAATTAAAGTAGAGTCTATACAAAATCTGTTGGATTCAGATGAAATGAGCCACTTGAGAGGCATACCGAAGGTGTTTATTATACAAGCTTGCCAAGTTGAGGATACACCTCATCCTACATTTGCTGCTGACAATGTCCAAACAAATTACTACTTGGGAAAATTAGACTTCCTTATTTACTGGGCCACTGCACCTGAATATGAAGCCTTCAGACATGAGCAGACGGGGTCATTATTCATTCAAGCTCTTTGTAAACTGTTGCGTCAAAGAGCTAAACATGATCACTTACATGAAATCTTTACACAAGTAAATAACAATGTTACCAATCTGTGCACTAAGTTGCAGCGTGCACAAGTACCTCTCTTCAAAAGTACTCTGAGGAAAAATTTATATTTACAAGTGCCAGAATAA > M. sexta-Relish F (MsRelF); Msex2.08004-RE(SEQ ID NO: 34)ATGTCCTCTTGTCCAAGCGACTATGATCCCAGTGAATCGTCCAAATCTCCACAAAGTATTTGGGAGTCAGGAGGATACAGTTCTCCGTCGCAACAAGTTCCTCAATTGACTTCTAACTTAACAGAATTGTCTGTTGATCACAGCTATAGATACAATGGAAATGGACCATATCTACAGATCACAGAGCAACCACAGAAATACTTTCGGTTCCGTTATGTTAGCGAGATGGTGGGAACACATGGATGTTTGCTTGGCAAATCTTATACAACAAACAAAGTTAAAACTCATCCGACAGTTGAACTCGTGAATTACACCGGTCGAGCCCTGATAAAGTGCCAACTGTCGCAAAACAAGAGCGAAGACGAACACCCGCACAAACTGCTCGATGAACAAGACAGAGACATGAGCCACCACGTTCCCGAGCACGGCAGTTATAGAGTGGTATTTGCCGGTATGGGTATAATTCATACTGCCAAAAAGGAAGTTGCAGGGTGGCTCTATAGAAAATATATACAGCAGAACAAGAATGAAAAGTTTAATAAGAAAGAGCTCGAAGCGCATTGTGAGAGGATGTCCAAAGAGATCGATTTAAACATAGTTAGACTGAAGTTTAGCGCTCACGATATTGACACTGGCATTGAAATTTGCCGGCCAGTGTTCTCTGAACCCATTTATAATTTGAAGTGTGCGTCTACGAATGATTTGAAAATATGCCGCATAAGCCGTTGTTACGGTAGGCCGAGAGGCGGCGAAGATATCTTCATATTTGTCGAAAAGGTCAACAAGAAAAACATCCAAGTTCGGTTCTTTAGACTGGAAAACGGGGAGCGCACCTGGTCAGCGATGGCGAACTTTCTGCTAAGCGATGTTCACCACCAATACGCTATCGCTTTTAGAACGCCACCGTACGTCAATCACCAAATTTCTGAAGACGTGCAAGTTTTTATAGAACTCGTACGCCCTTCAGACGGTAGGACGAGCGCTCCCATGGAGTTCACATACAAGGCTGAGCAAATCTATAAACAGAACAAGAAACGTAAAACTACTTCGTCGTACTCGTCGCTCGACAGCTCCTCAGGTTCGGCCGGTTCAATTAAAAGCATCAGCGAACTGCCCGCACCCGTTGTTTTTGCTGAAAACTTACCTGAAAACAATGAGCGTATTATAGATATACCCGTACAACAGAATATGCTGTACCAGGTTCTGCCAAGTCAATGTGATTTAGCCGACGCGTTCATGGAGGTGGACAGCAAGGGAAGTCCGGCCAGCGTCGTGGACCCGATGTGGGCCGGCGCCGACGTCGGGCTGCAGATGGCGTCGAGCCTGCCCGCCATCAAACTCGGCTCCACCGAACTAGAGAGCTTGGCGCACCGCAGCCGGGACGGAGTGCCCATGGACAAAGAATTCCTTGACAATTATCTCAGCTCTCTCAACTCGCTTGGTGAACTTAACGACGATGATGAGATCAATCATATGCAATATGTGCGCTCGTTACAGATGGTCCACGCCGACTCGGCACGACGAAAGGCTGAACCGCAACCCAAAGACACTGTAACCAAACCGACTCAATCTTCCCCGAAGGACACAGACTATGGCGCCCAGTCGGGACGCCCCACTGAATATTCTGCTTATTATAAAATGGAAGACGGCGTGGAAGTCAAAAAACTGGTGAAAGAATTGTGTAGTATCATACAGAACAAAGCGGGATATAAAAAACAAGAAGTGAGAAACAAATTGGAGAGGCTGTTCACCTACCGTCTGTCCAACGGAGATACATTCCTTCATATGACATTGTGCAGCAATCAAAGTAGTTTTGAATACATCGTCAAAATCATACATAGCGTGAAAATGACACATCTATTGGACTATTGTAATAATAAACAGCAAACCATATTACACATGGCTATTGTAAACGACCTGCCTCGAATGGTCTCTTTACTTATTGCAAAAGGTTGCAATCCAATGAATAAAGACAGCGAGGGCGACAATGCGGTGCACTACGCTGTGAGGAGCGAATGTTGTTTAGAAGCATTATTGGACGCCATCAAAAATAACAATGTTCGGTGCGATTTGAACGACTGCAATAACGAGAAGCAGACGGCGCTGCACCTGTCGACGAGCGGCGCGAGCGCGCGGCAGCTGGCGGCGCGCGGCGCCGACCCGCGCGTGCGCGACGCGCAGGGCCGCACGCCGCTGCACCTCGCCGCCTACGACGACAACTGCGACGTCGTCAGGGCGCTGCTCGAGTTCGTGTCGCCCTCGGAAATAGACGTTGTGGACGGCTGTGGCAACACTGCGTTACAGATCGTCTGCGGCGGCTCCGTTAAGAAGAACACTTTGGAAATAGTAAAACTGTTGCTCCAAAAAAAGGCTGATCCGTTGAAGCAAGATGGGCACAACATATCGGCGTGGAAGATGGCGCGCGAACACTCCGAAATACGGGACGCGATGAAGGACTATGTCCCCGCCGCCGCGTACGAGGAGGACACCAAGTCGGAAATGGACGATGAGTTCGAATCCGCTGATGAGGAGGACTACCGGATGGGTTCCGGATCGGGCGCTGTGAGTCTGCCGGAGCTGGGCGCATACGTGGAGGCGCTGAGCGCGGCGCTGGAGGCGCGCGGCGCGTGGCGCGCGCTCGCGCACCGCCTCGGCCTCGCCGCCGCGCTCGACTGGTGCGCGCGACAGCACGCGCCCGCGCGCACGCTGCTGCTGCACCTCAAGGAATGCAGAAACGACATATCCTCGAAAACGTTGGCTGTAATTCTAGAAGATATGGGAGAATTAGAGGCTGCTTCAATTATAAGGAGACACATAGAG TGA> M. sexta-Cdc42 (MsCdc42); Msex2.04668-RA (SEQ ID NO: 35)ATGCAGGCGATCAAGTGTGTCGTCGTCGGAGACGGTGCCGTCGGTAAAACATGTCTGCTCATCAGCTACACGACAAATGCCTTCCCCGGAGAATACATACCTACAGTATTCGACAATTATTCAGCGAATGTGATGGTGGACGGGAAGCCGATCAACCTGGGCCTGTGGGATACGGCGGGGCAGGAGGACTACGACCGGCTGCGGCCGCTGTCCTACCCACAGACCGACGTGTTCCTTATATGCTTCTCGCTCGTCAACCCGGCTTCGTTCGAGAACGTTCGGGCTAAGTGGTACCCAGAAGTGCGGCATCACTGCCCGTCGACGCCCATCATCCTCGTCGGTACCAAGCTGGACTTGCGCGAAGACAAAGACACCATAGAGAAACTTAAGGACAAGAAGCTCGCGCCTATCACTTACACACAGGGTCTGGGCATGTCGAAGGAGATCAACGCGGTGAAGTACCTCGAGTGCTCTGCGCTGACGCAGAAGGGTCTGAAGACGGTGTTCGACGAGGCCATCCGCGCCGTGCTGTGTCCCGTGCCGCCCCCCAAACAGAGCCGGAAGTGCACGCTGCTGTAA> M. sexta-DSor1 (MsDSor1); Msex2.00725-RB (SEQ ID NO: 36)ATGAGTAAGATGTCAAAGAATAAATTAAACTTGACCCTGCCACCAGGGTCAATAGACACAGCACCAGCCATCACACCATCCAATATGACACCACAGCTGAAGTCCGCAACAGCTACGGAGCGTCAGGGCTTGGCTGGTAAATCGAAAACCAGCATCGAAGCCCTGACAGAGAGGTTGGAGCAAATCGAGATGGACGACACACAGAGACGGAGAATAGAAGTGTTTCTGTGTCAGAAGGAGAAGATCGGGGAGCTTAGTGATGATGATTTTGAAAAGCTTGGAGAGTTAGGCCAAGGCAACGGTGGCGTTGTAATGAAAGTCCGTCACAAGTCAACCGGTCTGATAATGGCGCGAAAGTTAATCCATCTGGAAGTCAAGCCGGCAATAAAGAAGCAAATCATCAGGGAGTTGAAAGTCTTACACGAATGTAACTTTGCGCATATCGTCGGCTTCTACGGGGCCTTTTATAGCGACGGCGAGATCTCGATTTGTATGGAGTACATGGACGGTGGGTCCTTAGACCTTATACTGAAGAAGGCCGGCAAGATTCCTGAATCTATTCTAGGAACAATAACATCCGCCGTGCTGAAAGGTCTGAGCTACCTCCGGGACAAGCACGCCATCATGCATCGCGACGTGAAACCATCAAACATTCTGGTGAACAGCAACGGCGAGATCAAGATATGCGATTTCGGCGTGTCCGGTCAGCTGATCGATTCCATGGCCAACTCTTTTGTCGGCACTAGGAGTTATATGTCTCCCGAACGTCTCCAAGGCACGCACTATTCAGTCCAATCTGACATATGGTCGCTCGGGCTGTCGTTGGTAGAGATGGCAATCGGAATGTATCCCATACCGCCGCCGGACGCGAAGACCCTGGCTGCCATCTTCGGTGGACAGAATGAAGATCATTCTCCTGGTCAGGCGCCGAACTCGCCCCGTCCGATGGCCATATTCGAGTTGCTGGACTACATCGTGAACGAGCCGCCGCCGAAGCTGCCCGCCGGAATATTCTCCGACGAGTTCAAGGACTTCGTCGACCGCTGCTTGAAGAAGAATCCAGACGAACGAGCCGACTTGAAGACTTTGATGAATCACGAATGGATACGCAAAGCGGACGCAGAAAAGGTGGACATAGCGGGGTGGGTGTGCAAGACAATGGACCTCATGCCTTCCACTCCAAACTCTAACGTGTCTCCTTTTTCTTCATAA > M. sexta-FOS (MsFOS); Msex2.09858-RA (SEQ ID NO: 37)ATGCAGAACATCGATCCTCTGGAGATCGCCAACTTCCTCGCCACGGAGCTGTGGTGCCAGCAGTTGGCGAACCTCGAGGGCCTCCAGTCGGGGGTCCCGACTCGCACAACGGCCACCATCACGCCGACGCAACTGCGCAACTTCGAGCAGACTTACATCGAGCTGACCAACTGCCGCAGCGAGCCCACCACGCACGCCGGCTTCGTGCCGCCGTCAGTCACGCACGCCAACAACTACGGCATCCTGAATCCGACGGCGTATTGTGATTCGGGCCCGACGACGGCGCTGCACGTGTCGCCGGGCCCGCTCTCCGCCAGCGGCGACAGCAGCAGCAGCCCCGGCCTGCCCACGCCCAAGCGGCGCAACATGGGCGGCCGCCGGCCCAACAAGGCGCCGCAAGAACTCACGCCCGAGGAAGAAGAGCGCAGGAAGATCCGCCGCGAGCGAAACAAAATGGCCGCCGCCCGTTGTCGCAAGCGCAGACTCGACCACACCAACGAGCTGCAAGAGGAAACCGACAAATTAGAGGAAAAGAAGCATGCGCTCCAGGAGGAAATCCGCAAACTGAACGCTGACCGGGAGCAGCTGCAAGTGATCCTTCAGAACCATATGGTATCATGCCGGCTCAACAAGAGATCCATCAGCCCGCCCGATGTGAAGCCCTTCCAGGACCCGTACGCCTACCCTGAGATACCCGAGGATGGCGTCCGTGTCAAGGTGGAAGTGGTCGACCCCTCAGTAGACACGGTCTTAGTGTTGGACAATATTTACTCAACGCCGCCGACTGACAAAAAAATTATGCTGTCGTCGGCCAACCCAGCCGTGGTGACGAGTGGGTCGCCCGCCGCCCTGGAGACCCCGCCGGCGATAGTGCGTCCCAACAGACCCAACTCCCTCCAGGTGCCGCTCAGCCTCACACCAGCACAGTTACACAACAACAAGGCGCTGGGAAACAACAAAATAGCCGGCATAGAGATAAGCACGCCGAGCAACGGCATCCCGTTCAACTTCGAGAGCCTGATGGAGGGCGGCACGGGGCTGACGCCGGTGCACGGCCACGCGCTGCCGCTGCCGCACCCGTGCGCGCAGCAGCAGCGCGCCGCGCCCGACGCCTCGCCCGCCGAGCCGGCGCCGAGCTCGCTGGTGAGCCTCTGA > M. sexta-Jra (MsJra); Msex2.12422-RB (SEQ ID NO: 38)ATGGTTCGGCACTCCGGCCACGGCATGGAGACCACTTTCTATGACGAGCAGTATCCCATCAGCGGCCCCGTGGAGAACCTGAAGCGGCCCCTCACGTTAGACGTGGGGCGCGGCGTGAAGCGCGCCAGGCTCGGCGGGGCACCCGTACTCTCATCTCCAGACCTACAGATGCTGAAGCTCGGCTCGCCAGAGCTCGAGAAACTGATCATCCAGAACGGCTTGGTGACGACGGCCACCCCCACTCCAGGTGCGCCGGTGCTGTTCCCCGCGGTCGCGCCTACCGAAGAGCAAGAGATGTACGCGCGGCCATTCGTCGAGGCGCTAGACAAGCTGCACCACGGCGAGGTGACCCCGATCGGGCGGCGAGTGTACGCCGACCTGGACCGGCCGCTCGAGCGGTACCCCACGCCCGTGGTGAAGGACGAGCCGCAGACGGTGCCCAGCGCCGCCAGCTCGCCTCCACTTTCCCCTATTGACATGGACACGCAGGAGCGGATCAAACTGGAGCGCAAGCGACAGAGGAATCGAGTGGCCGCTTCCAAATGCAGGCGGCGCAAGCTCGAACGCATCTCCAAGCTGGAGGACAAGGTGAAGATCCTGAAGGGCGAGAACGCGGAGCTGGCGCAAATGGTGGTGAAGCTAAAAGAGCACGTACACCGACTGAAGGAGCAAGTGCTGGAGCACGCCAACGGCGGCTGCCACATCGAGTCGCACTTCTGA> M. sexta-Caudal (MsCAD1); Msex2.04570-RA (SEQ ID NO: 39)ATGGTGAATTACGTTAATCCCCTCGCCATGTACCAAGGCAAGGGCGGGCAATACGGCGGCGGGTGGTACGGCTGGCAGCATCAGAACTTTGAAGAACAACAATGGTGTGCTTGGAACGGTGCGCCGGCGGGTGGCGAGTGGGCGCCAGATCCACATCATTTTCCCAAAAGAGAACCTGGAGAGAGAGAAATAGCAGACATGCCATCACCGGCACGAGGAGACTTGGCAAGTCCAGCAGAAGGTTCGCCGAGCTCGGGGTCGAGGCCGTCGCAGCCGCCAGCACCGCCGCGTTCCCCATACGAATGGATGAAAAAACCCAACTACCAGACACAACCTAACCCAGGTAAAACGCGCACAAAAGACAAATACAGGGTGGTGTACAGTGATCATCAGAGGCTGGAGTTAGAGAAGGAGTTCCACTACAGCAGGTACATCACTATTCGTCGCAAGGCAGAACTCGCCGTCAGCCTTGGCCTTTCCGAACGACAGGTCAAAATTTGGTTTCAAAATAGACGAGCCAAGGAAAGGAAACAGGTGAAGAAACGCGAAGAAGTGGTGATGAAAGAGAAAGGCGACCATGCATCTCTACAGCACGCGCAGCTGCACCATGCCACCATGCTGCATCACCAGCAGATGATGAACGGCATGATGCACCACCACCACTACCACCAAGGCGTGTTGCAAGGCGTGCCGGAGCCGCTGGTGCCGGGCGTGCCGCCCGTGCCGCTGCTGTGA > M. sexta-Atg8 (MsAtg8); Msex2.12227-RA (SEQ ID NO: 40)ATGAAATTCCAATACAAAGAAGAACATTCTTTCGAAAAGAGGAAGACTGAAGGCGAAAAGATTCGCAGGAAATACCCGGATCGCGTTCCAGTAATTGTAGAGAAAGCCCCGAAGGCTAGATTGGGAGACCTCGATAAGAAGAAGTATTTGGTGCCGTCTGATTTGACTGTCGGACAATTTTATTTCCTAATTAGGAAACGCATCCATCTTCGGCCTGAGGACGCATTGTTCTTTTTCGTGAACAATGTTATTCCACCAACATCCGCCACCATGGGCTCTCTGTACCAGGAACATCATGACGAAGATTTCTTCCTCTACATTGCATTTTCTGATGAAAATGTTTATGGATTTTAA> M. sexta-Atg13 (MsAtg13); Msex2.06273-RA (SEQ ID NO: 41)ATGGCACCCGAAGTGGCTTTTTCTAACATAAATGACAAGAATGAATTTACTAAATTCACAAAATTCTTAGCTTATAAAGGCGTTCAAGTTATTGTAGAATCGAGGAAGGGTGTAAAAATAGATCCTAATAGTAAACCAAGATCATCAGACACTGATTGGTTCAATCTTCAAATACCAGACTCTCCAGAGGTTAACCAGGCAACTAAGAATGCATTGCCTTCAGACAAGGTGTTAGAGATTATCAAAGCCCAACTGCACGTGGAAATATCAGTACAAACCGAGGATGGCGATGAAATGGTTTTGGAGTTGTGGACCCTTGAACTTGACGAAACTCAGTTTGACACTTCTGTTAAAGCCACGAACACAGTTTACTTCAGAATGGGAATATTACTTAAGTCGCTTATAACTATAACAAGAATTACTCCAGCGTACCACTTGTCGAGGAAGCAAAGAACAGAGTCGTTCACAATATTCTACAGAGTATACAATGGCGAACCGAAAGTAAAAGCGCTTGGAGAATCAGTGAAGAAAATTCAAGCTGGAATGCTCAAAACTCCACTTGGAGGGATAATATTCTCCGTGGCCTACCGCACAAACTTCTCCATTTCGCCAAACAGATCGGAGAAGGACAAAACATTGCTTTTGAAGAGTGATCATTTCGAACTGAGTCCAAAACATGTGATATTTGAATCCAAGAAAAAGAAAGACGAGAAAAAAGAATACAAGCCTCTGAAACCTGTTGATTTGAATAAGCCACTTCGATTAGCCGCATTTGTAGACGAGGATGTTGTAAAAAAGGCCTTTGATGACTTCATGGAGAAGATGCCGATTCCCAAATACCGAGTGATACGTAGGGAGAGAAGTCCAGAGAGCGACAAGCCTATAGTATCCAAAAGTACTCAGGATTTGGACGCTTCTGTGACGTCAAAGAGTCCGACGTCAATGGAAATGCCACCTAAAAAGTTCACAGGCTTTCGCAATGAGAACGAACCTCCATTAAAACTTCTCCATTTCCCATTCGCTGATAACCATCCGATAAGAGAACTGGCCGAGTTTTACAAAGAGTTCTTCAACGCCCCCCATTTGAAGTTAGCTGATGACGTTAGCCTGAAATCGGGCAGTGCTGAATCGGTAAAAGAGGTGATAGAGATTACAACTGAAGATTTGTCGAAAGATCTCGAATTGTATGAGAACTCGGTGTTGGAGTTTGATGAGCTCTTGGCAGATATGTGCCGATCGGCTGAGTGGAGTGGGAACTAG> M. sexta-IAP1 (MsIAP1); Msex2.05607-RA (SEQ ID NO: 42)ATGGCATCAGCTGGCGCCGTACCGAATATTCTAGTGTCGTCGTCGCTTTTCAAGACCACTCCTCGGGATCCTAAAATTCGTCGAAAAACAAGCCCTTTGAAGTTACCACCTTGTGACACACATATAGGCTCGCCTCCACCATCATCCTCTCCGACCTCCTCGTCAACTGATAAAACCGATAATCATGATACTTTTGGTTTCCTTCCTGACATGCGtgatatgcgCCGCGAAGAAGAACGACTGAAAACTTTCGATAAGTGGCCTAGCACGTACGTGACACCTGAAGAGTTAGCTCGTAATGGTTTCTACTACCTTGGGCGCGGTGATGAAGTTCGCTGTGCATTCTGTAAAGTAGAAATTATGCGATGGGCGGAAGGAGACGACCCTGCGAAAGATCACAAACGATGGGCGCCCCAATGTCCGTTTTTACGTAATCTTTCGAACGGTACTAATGGCAGCGGAGAAGGTAGTTCGGAAGGTCGCGACGAGTGTGGTGCGCGAGCAGCGACGCGGGAGCCTGTGCGTATGCCCGGACCTGTGCATCCCCGCTATGCAACAGAGTTATCGCGTCTCGCCAGTTTCAAGGATTGGCCGCGTTGCATGCGCCAAAAACCAGAGGAACTCGCGGAAGCAGGATTTTTCTACACCGGCCAAGGGGATAAAACGAAATGTTTCTATTGCGATGGAGGTCTCAAAGATTGGGAAAACGACGATGTTCCATGGGAACAGCATGCCCGTTGGTTCGATCGTTGTGCGTATGTGCAACTTGTTAAAGGCCGTGATTATGTCCAGAGGGTATTATCGGAAGCGTGTGTTATACGTGCCACCGACGAAAAGCCTGCGCCTGCACCTCAACCGTCCCAACCAAATGTCTCAGTTGTATCAGAAGAAAAACCTGTCGAAGAGGCTAAGATATGCAAAATATGTTATTCCGAAGAACGAAACATATGCTTTGTGCCGTGCGGTCATGTGGTCGCTTGTGCAAAGTGTGCCTTATCTACTGATAAATGCCCAATGTGCCGGAGGGCTTTCACAACTGCACTGCGACTCTATTTTTCGTGA> M. sexta-Chitin synthase 2 (MSCHS2); AY821560.1 (SEQ ID NO: 43)ATGGCCGCAACTACACCAGGTTTTAAGAAGTTAGCAGACGATTCTGAGGATTCAGATACAGAATACACCCCGCTGTATGATGACGGTGATGAAATAGATCAAAGAACTGCACAAGAAACAAAAGGATGGAATCTATTTCGAGAGATTCCGGTGAAAAAGGAGAGTGGATCTATGGCCACAAAAAATTGGATAGAAACAAGCGTAAAAATCATAAAAGTGCTTGCCTACATATTGGTTTTTTGTGCTGTACTGGGTTCCGCAGTCATAGCTAAAGGAACTCTTCTATTTATTACGTCACAACTGAAGAAAGACAGACAAATTACTCACTGCAATAGACGACTTGCTTTAGACCAACAGTTCATAACGGTACACAGTTTAGAAGAAAGAATAACATGGCTATGGGCAGCACTTATTGTATTCGGTGTGCCGGAGTTAGGGGTGTTTTTGAGATCCGTCAGGATATGCTTCTTCAAAACTGCCAAGAAACCAACCAAAACACAGTTTATTATTGCTTTCATAACAGAGACACTACAAGCAATAGGAATAGCAGCACTTGTATTAATAATTCTACCAGAATTAGACGCTGTGAAAGGAGCCATGTTGATGAACGCCACGTGCGCTATCCCTGCATTGCTAAACATTTTCACGAGAGACCGAATGGATTCTAAGTTTTCTATAAAATTGATATTGGATGTATTGGCGATATCGGCACAAGCCACGGCGTTTGTTGTTTGGCCTCTTATGGAAAGAACGCCAGTTCTATGGACCATACCAGTTGCATGTGTGTTAGTGTCTCTAGGCTTCTGGGAGAATTTTGTTGACACCTACAATAAAAGTTATGTTTTTACGGTGCTGCAGGAACTACGCGACAACCTCAAGAGGACTCGGTACTACACTCAGCGGGTGCTATCTGTTTGGAAGATTATAGTGTTTATGGCATGCATTTTAATATCGCTGCATATGCAAAATGACAATCCGTTTACCTTTTTCACTCACGCCAGCAAAGCCTTTGGAGAGAGACAGTATGTCGTTAACGAGGTTCTAATAGTAGTCCGAGATGACGAAACCATAGGCTATGACGTCACCGGAGGTATATTCGAATTGGACGCGATATGGACCTCAGCATTGTGGGTCGCATTAATTCAAGTGGGAGCAGCCTACTTCTGTTTCGGAAGTGGCAAGTTTGCTTGCAAAATTCTTATACAAAATTTTAGTTTCACTTTAGCATTGACTCTCGTCGGGCCCGTGGCAATCAACCTCCTTATTGCTTTCTGCGGAATGAGAAATGCAGACCCTTGCGCTTTCCATAGAACTATACCTGACAATTTGTTTTACGAGATACCACCTGTGTACTTCTTGCGGGAGTACGTGGGCCACGAGATGGCGTGGGTGTGGTTATTGTGGCTCATATCTCAAGCGTGGATCGTGTTTCACACGTGGCAGCCGCGATGCGAGCGCCTCTCCGCAACTGACAAACTGTTTGCCAAACCGTGGTACATCGGACCGCTAATCGACCAATCGTTGCTGCTAAACAGGACTAAGGATTTGGATAATGATTGCCAGGTTGAGGATTTGAAGGGTCTTGGCGACGATTCGTCGGTTGGAAGCGATCTTGCCATCGTAAAAGATATCAAACCGTTCGATTCGATAACCAGAATACAAGTGTGTGCGACAATGTGGCACGAGACCAATGAGGAGATGATCGAGTTCTTGAAGTCGATATTCCGCCTCGACGAGGACCAGAGCGCGCGCCGCGTGGCGCAGAAGTACCTCGGCATCGTCGACCCCGACTACTACGAACTCGAGTGTCATATTTTCATGGATGACGCTTTTGAAATATCCGATCACAGTGCCGAAGACTCGCAGGTGAATCGCTTCGTGAAGTGCCTAGTGGATGCGGTGGACGAAGCGGCTTCCGAAGTGCATCTGACTAACGTGAGGTTAAGGCCCCCCAAGAAATACCCCACCCCGTACGGCGGAAAACTAATTTGGACCATGCCAGGGAAAAATAAGTTGATTTGCCATTTGAAAGATAAATCCAAGATTCGGCACAGAAAGAGATGGTCGCAGGTTATGTACATGTACTATTTCCTGGGGCATCGCTTGATGGACCTGCCAATATCCGTGGATCGTAAGGAAGTGATTGCCGAGAACACGTACCTATTAGCTTTGGATGGAGACATCGACTTCAAGCCGAGCGCTGTGACGTTGCTGGTCGATCTTATGAAGAAGGATAAGAACTTGGGCGCCGCTTGCGGTCGCATTCATCCTGTCGGCTCTGGTTTCATGGCCTGGTACCAGATGTTCGAGTATGCTATTGGTCATTGGCTGCAAAAGGCGACTGAACACATGATCGGCTGCGTACTCTGTAGTCCGGGATGCTTCTCCCTCTTCAGAGGAAAGGCGCTTATGGACGACAACGTCATGAAGAAATACACACTCACTTCTAACGAGGCTCGACATTACGTGCAATACGATCAAGGCGAGGACCGTTGGCTGTGTACGTTGCTGCTGCAGCGCGGGTACCGCGTGGAGTACTCGGCGGCGTCGGACGCCTACACGCACTGCCCCGAGCGGTTCGACGAGTTCTTCAACCAGCGCCGCCGCTGGGTGCCCTCCACCATGGCCAACATATTCGATCTGCTCGCGGACTCCAAACGCACCGTGCAAGTCAACGACAACATTTCCACTCTGTATATCGTCTATCAGTGCATGCTTATGATGGGTACGATTTTGGGTCCGGGAACAATCTTCCTGATGATGATTGGCGCAATAAACGCTATAACAGGCATGAGCAATATGCACGCACTTCTCTTTAACCTGGTGCCCGTGCTTACGTTTTTGGTTGTCTGTATGACATGCAAGTCCGAGACTCAGTTGATGCTCGCGAACCTCATTACCTGCTTTTATGCGATGGTAATGATGTTTGTGATCGTCAGTATCGTCCTACAAATATCACAAGATGGTTGGCTAGCGCCATCTAGTATGTTCACTGCGGCAACATTTGGAATATTCTTCGTAACAGCGGCTTTGCATCCACAAGAAATAATATGTTTGTTGTACATTTCCATATATTACATCACAATTCCGAGCATGTATATGTTGTTGATTATCTACTCCCTATGCAATTTGAACAACGTCTCGTGGGGAACTCGAGAGGTGGCTCAGAAGAAGACTGCAAAGGAAATGGAAATGGATAAGAAAGCAGCGGAAGAAGCAAAGAAGAAGATGGATAATCAAAGCATAATGAAATGGTTCGGCAAGTCGGACGAGACGAGCGGCTCGCTGGAGTTCAGCGTGGCGGGACTGTTCCGCTGCATGTGCTGCACCAACCCTAAGGACCACAAGGACGACTTGCATCTCTTGCAGATCGCCAACTCCATCGAGAAGATCGAAAAGAGATTGTCGGCACTTGGCGCGGAGGAGTCCGAGCCGGCGCAGGCGCAGACGCGGCGCCGCTCGTCGCTGGGGCTGCGGCGCGACTCGCTCGCCACCATGCCCGAGTACGCCGACAGCGAGCTGTCCGGAGACATTCCTCGCGAAGAAAGAGACGATCTTATAAACCCCTATTGGGTGGAGGATCCCAATCTGCAGAAAGGTGAAGTAGACTTCTTGACGACGGCTGAAATCGAATTCTGGAAGGACTTGATTGATGTTTATTTGAGGCCTATCGATGAAAACAAGGAAGAGCAGGAACGTATCAAAACCGATCTAAAGAACTTGCGTGACACGATGGTGTTCGCGTTCGCCATGTTGAACTCGTTATTCGTGTTGGTGATATTCCTGCTGCAGTTCAACCAGGACCAGTTGCACATAAAGTGGCCGTTCGGGCAGGATGTCGCGCTTTCTTATGACAAGGAAAGGAATGTTGTATTGGTGGAGCAGGAATTCCTTATGTTGGAGCCTATAGGTTCCCTGTTCCTCGTGTTCTTCGGGTTTGTAATGTTGATACAGTTCGTGGCGATGTTGTCCCATCGTTCGTATACTATTACGCATTTGCTCTCCACAACAGAGCTTCACTGGTATTTCAGCAGACGTCCGGACCAGATGTCAGATGAAAACCTCTTGGAAAGGAAGGTGGTAGAAATAGCGAGAGAGTTGCAGAAGTTAAACACGGACGACCTAGACCGCCGCGCGGTCGAAACTAACGACGTGTCGCGGCGAAAGACTCTACACAACCTAGAGAAGGCGCGAGACACCAAGCACAGCGTGATGAACCTTGACGCTAACTTCAAGAGGCGACTGACTATACTACAGAGCGGTGATCCTAACGTGATATCTCGGCTGTCATCGTTGGGCGGCGATGAGGTTACTCGTCGCGCCACGATACGCGCATTAAAGACGAGGAGGGACTCACTGCTCGCTGAAAAACGACGCTCCCAGCTGCAAGCGGCGGGCGACGCTACAGGCTACATGTATAACCTGTCAGGCACTGCGGTGAACGACATGAGCGGCCGAGCTTCGACGGCCAGCGCCTACATTAATAAAGGATACGAACCCGCTTTCGATAGCGACGACGACGAACCACCGCGTCCGCGCAGGAGCACTGTACGCTTCAGAGAAAACTACACGTAA> M. sexta-Beta-fructofuranosidase 1 (MsSuc1); GQ293363.1(SEQ ID NO: 44)ATGTACATTAAAACAGCAACATTTTTGCTGTGCGTTTTCCTTGGTAGTGTATCGTCATGTTGCGTTAATGGGCGGTACTACCCGAGGTACCATTTGTCGCCACCGCATGGCTGGATGAACGACCCCAACGGATTCTGCTACTTCAAAGGTGAATACCATATGTTTTACCAGTACAATCCCATGTCAAGTTTGGAGGCTGGCATAGCTCATTGGGGTCATGCGAAAAGTAAAGATTTGTGCCATTGGAAACACTTAGACCTCGCCATCTATCCTGATCAGTGGTACGATCAAACGGGAGTATTTTCTGGAAGTGCGCTAGTAGAGAATGACGTCATGTACCTTTATTATACTGGAAATGTAAATCTTACTGATGAAATGCCATTTGAGGGACAATTCCAAGCTCTTGGTATCAGTACTGACGGTGTCCACGTAGAAAAGTATAAAGACAATCCAATAATGTACACGCCAAACCATCAACCTCACATCCGAGACCCAAAAGTTTGGGAACACGACGGCTCTTATTATATGGTCTTAGGAAACGCATATGATGATTATACAAAGGGCCAAATAGTTATGTACGAATCATCAGACAAGATCAACTGGCAAGAAGTAACTATACTATATAAATCAAATGGATCTTTCGGTTACATGTGGGAGTGTCCAGATTTATTCGAAATAGACGGCAAGTTTGTACTTCTGTTCTCTCCTCAAGGCGTGAAGTCTGTGGGCGATATGTACCAGAATCTGTATCAAGCAGGATACATCGTCGGAGAATTCGATTACGATACTCATTCATTCACAATACTAACCGAATTCAGAGAATTGGATCACGGTCATGATTTTTACGCTACACAAACAATGAAAGATCCTAGTGGAAGAAGAATAGTCGTTGCTTGGGCAAGTACTTGGGAGTATGCTTATCCTGAACGAGCAGATGGTTGGGCTGGCATGCTCACACTACCTAGAACTTTAACTTTGACAAAAGATTTAAGACTAATCCAAACTCCAATTAGAGAGATCGATCAAGTTTTTAGAAGAAGACTATATTCAGGAAAAGCCTCAGCAGGCAAAACTGTCGCTTTACCAGACAAAGCAGGGAAAGTAGAACTGAAATGGGATACACCAAGAAATATAAAGGTAGTTATAGAATCTCAAAATGAGTGCCAAAACGTAGTAATCAGTTATGATCACGAGGATGGTACTATTACTTTGGACAGAGGAGGCGATGACGCAATCCGCCGCACTCACTGGGATCCTCGAGGTCACCTCAAATGGACCATTTTTATTGACGCAAGCTCCATAGAACTCTCTTGTGGTGATGGAGAAGTATGGTTTACAAGCAGATTTTTCCCTGAAGGAGTCGTATCTGTTCGCCTTGGAGAAGATACTTGTGTTGATAAGTTTACCGTGCATTCTATTCGCCGTACTACTCCAGACCCCGAGGCTCATTGTCGTTGTGAATCAGAAGAATAA> M. sexta-Hemolin (MsHEM); M64346.1-UTRs 5′UTR (SEQ ID NO: 45)TGTTACATTAATTATAAAAAAAAATACAAAAAAGAAATATATAAGTAAGTACTAATAATATGGTCTAGTTTATTATAAGTCTTAGAGACTAACGATAAATAATAAATTTCTCATGAACCACCTATTTATTTATTTGAAAAGCATTTTAAATATATTATAGATTTAAACGTAACGAAGTCTTTAAACTCGCGGGTAAAAACGCATACTTACTTGACTCTACATCGGCGACACACGAAAAACTTATTCGTACTGTTTTTTCATTGAATATGTTATGAAAAAGAAAACTATATGTAATCGCAGTCACATATATAAAACTTTATAAAAAAGTTAACAGAGATAAATAACTTGTTTGGTGTTCGTCATAGCAATAGAGTAGCCGCGGCGCCTAAGGCTACGAAATAAGCTCATTGAACTGTAATGGAGTGTGCCTAGTTGTTTCTCTTCTTACCAAGACTCAAGAGGGATTTGGAGCTTCATCTACGATTATTATACCTACCTACGTATAGACTATAATGGATAGTAAAAATAGAAAGTAATTTCTTTTATATCAACTTTTGTATTCATATGTAAGTACTTACTTATAATTATTTATTTTTTTAG GTGAAA3′UTR (SEQ ID NO: 46)GTTAACAATAAACAATACTGTTAACCGTACGTAGTGTTAAATAATACAAATATATGTATTTTACAATAAGATTTCCTGTTTTTAAATTATCCTCACGTAACGTATTGGGTATCTAAGCGGTTGAGCAGTGAGATAAGTCTACCCCAAAGTAGTCCGTTCATCAAACGACATAACCATCTCGTCATTTAGTTAAAACAAAAAAAGAATAATGATAAAAGATATAAAATTCTGTATAAGAACCAATACCTAGCACCTTTCTCACATTGCCAAACATACATTAAAAAACAGTATTATGCTTTTTTCGCTCTTTTATTCTATATTTTATATTAAAAAATACCTATAGTAAATACTGTTTTCATCGTCGCCGGTATTCATACACGTAGTATACGTACCTAACTCTGAAACTACTGGTTTGAATTG AAAAA> M. sexta- Serine Proteinase homolog 3 (MsSPH-3); AF413067.1 5′UTR(SEQ ID NO: 47) AGCGCTTTGGGAACGTTCCGTGGCACGATA 3′UTR (SEQ ID NO: 48)GATGGTGGAATGTTTAAAAATACAAGAGTGTGAGGCAGTGATTAATGGTCTGTAATATCCGATTCTTGCTGGCTTCTGTTTCGTAATTAACGTAAAATCTAATTATCATTAGAATATTTTGGAAAACCTTAGAGTTACCTTCGGAAAACTTCACCGTTTGCCGCTGGTGTGAAGATCTGTTCCCCCCATAAACTGATGGGAAATTGTATGTAGGTACTCGAGTTATAATTTCTTTTTATACCTGCGTTTAAGTTCCATTACAACTTTCTAATTTTATGAATAAATATCTAATAAACGATTAACAAAATTAAAAAAAAAAAAAAAAAA> M. sexta- Peptidoglycan recognition protein 2 (MsPGRP2); GQ293365.15′UTR (SEQ ID NO: 49)ACGGCATAAATTGTTAGGTCGTCTGAGAGGCGAGTGTTGTATTTTTAATTGCCAAAGAGGCTGCGAAGTTCGCAATAATCATTAGAAAA 3′UTR (SEQ ID NO: 50)AATGACCAAAGATAATACGACTGTTTTATAATTTTTGTTAATAAATGTTGTTGCATTATG GAAAAAAA> M. sexta- Beta-1, 3-glucan-recognition protein 2 (MsβGRP2); AY135522.13′UTR (SEQ ID NO: 51)ATTAAAACTAACGAAAAGACTCGTTTCCATAGTGGTATATTTTCAACTCGTCAATTTCAGGTACAAGCATGTTTGGTGGAAGGATATTATCGGCCCGAATAGGCACTGTACACCAAGTACAACCATGACTCATCAAAGGTGTCGCGTTCTGGAATCACCTGTATTTCCAACAGGCCGGCATAATTGTGTCGTCTAGCGAGGGATAACTCAATAGTCTATTTGAACGCCATTCTACTTACCATTAGGTGTAGTGAAGATATTTGGGGCTAGACAAAATGGAAGGAAATTTATGAGTTGCATCGAATGATTATCACTAAGTATTCGAATGATTCATTTCGTTGTTGGTGCAATAGGATGTCAAGGGTCAAATGGACATTAAAAGAGAGTTTAAGGTGTTTTTTTACAATTAACGTTGATCTATCACTTTACTTGCCTATTAAGTAAAGTATTATTTTGAAAAATACAAAATACTTGAGTTTATAGTATCTCTTGCGTTTATGGTACTGTCAAAAATATCAGTATTATTCATTTACAAAATTAAAATTTAATAATCTAAATTATTCTTCATGTAAATGATCATTTATACCTCTGCCTTGATTA TG> M. sexta-Relish family protein 2A (MsREL2A); HM363513.1 5′UTR(SEQ ID NO: 52)AGAGTACGTTCGATGGCAGTCTGTCGAACATTGGTAGTTTCCCGTTTGAGTGTTGTTTACTCCCTTTGAGGGATTAGTTTATTCTCCACGAATATAAACATCGGAAAATCAAAAACTAAGTTGATAAAAAGTTGTGTGCTCCGGTATAATTTTTTGGTATCAGTGACGGACAAAGGTGAT ATAAAA3′UTR (SEQ ID NO: 53)TATATTCATGAGAAGGGGACAACTAAGGATTGAATATCAGCAGAACTTGAGTTATGTTAATGAGGTATTTATATTAGCTTAATACTTAAAGGGGAAAATTCGATTAGCTTTTCAAATATACTTAAATTTTAGTTTTTGTCAAATATCGGTGTTTCCCATTTTGATATTTTTTTATCCATATTTTAATAATAAATATCTTGTCTTAGCAATTTTAATGGGTAATTATAAAGAAACTCACGCACCATAGTTGCACTAAACTACAAAATTACACAAAAAAAAAAAAAAAAAAAAAAAAAA> M. sexta-Dorsal (MsDor); HM363515.1 5′UTR (SEQ ID NO: 54)ATACAACGATATCACAGGCGTCCGGGGACGGACCAGTTTAAACAAACATTACAGTGAATAGCGATGTGATTATTTTCTTGCTTGTATAGAGTTAAATTTTTAAATTAGATTTAAATATTAAAATATTTGCGAATAAAA 3′UTR (SEQ ID NO: 55)ACTTAACAATTACATCTATAAATCTCTCCTCTATCAACCTAGTGGGCGTCCTCTACAACTACCACGTCTGGTATACAAATAGTGACAACCTGAAATTGTGAGATTTAAATTGTGTAGTTATGATAAATAAACAATAAATCCAAAAAAAAAAAAAAAAAAAAAAAAAAA> M. sexta-Toll receptor (MsTOLL); EF442782.1 5′UTR (SEQ ID NO: 56)GAAAAGTTATTCACAATATGACCCGAAGTGTCAGCGCCGCACATGCGCGGGAGCACTCGTACTTATACCAGACATTGTGACTAAATACCTAATTTTATGTTTTGCTCATCGCCCATTGCGCGACATTAAGCAGTATCCAGTAGTCAGTGATCAGTGTTACTACACTATTTTGCTTCTCAGCGATTAGTCAACACGCGCATATCGTTTACTTCAACAAACAATTATTACGCAAATCGTGATATTTGAATGTAGAGACACGAATCAAAGATTATTCGTTTAACGTTATTTTCGCGGGATGTTTCTCTGTTGGGGGGTACTGTTGCGTGGTGTGCAGTCAAAGGTTTATTTTATCGGGTATTGTGCTCTTTAAGTGTTGACGCGGCGGATTATCGTCGGTTAGACTAATAAATCGTGTCGATTTATGTGTGACCCACTACGGTGTCGTGCGACGTG 3′UTR (SEQ ID NO: 57)ATCCAGAACTTGCGTGTACCCTTCGATATACAGGGCTATTTTGACATCGTGTTACTAAATGAAACCACATTCTCGTCTTCACCTTTGCTGACATTGTGCCAAAAATCATCCATTAATAACGAATATTTCCACCAAAAAAAAAAAAAAAAA> M. sexta-Scolexin A (MsSCA1); AF087004.1 3′UTR (SEQ ID NO: 58)TAGACCATACCGTTGTCATTTTGGGCTGTAGTGTATAGATAATAAATATAGACGCGTGTACTGGTGTGACGTACGGAAGTGGAGAGTTGGGAGCGACAGCTCACCGCTCACTCCACTCCCGGCCGCCGCGCGAGTAGCAGTGTCAGTGATTGCAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAA> M. sexta-Hemolymph proteinase 18 (MsHP18); AY672794.1 5′UTR(SEQ ID NO: 59) GCCAGACGTTTCAGTTTGTTTCGAACT 3′UTR (SEQ ID NO: 60)TTATGAATAAATACAATTTAATAAGCATTATTTATTAAAAAAAAAAAAAAAAAAAAAAAA> M. sexta-Chymotrypsinogen-like protein 1 (MsCTL1); AM419170.1 5′UTR(SEQ ID NO: 61) TACTTCGGGCTACACAATGTACGTGAAAGTA 3′UTR (SEQ ID NO: 62)TCTCCGAACCGAACCTGATCTTAACTGTAAATAAAATAAGCGATACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA> M. sexta-Dredd (MsDRD); Msex2.04297-RC; Currently annotated asManduca sexta Caspase-6; HM234679.1 in NCBI 5′UTR (SEQ ID NO: 63)TTGGTAACCCTGTGGGAAGTCCCAAAGTCGAAGCTCGAGAACAGTTTTAAGCAGAGGTTTTAAGTCTAATTTATTGGTCTCTGGTTTTAAGTTAGTGTCATGTGTCAACAATAATAAAGCTTTATTTTTGGCTCTATTTATAAGTAAGGTATCATCCTTTCAGCGTTAAAACAAAGGGCGTAGCTCATGTTCATGTAGTTCTTTTAAATAAGTGTGAACTAAGTGTATATGTACATTTATTACTGGTGACA 3′UTR (SEQ ID NO: 64)CATAATATTTTTATTTAGATATATTGTAAGTACTAAGAATGAAGTGGTGATAGCTTAGTTGGGAGTGGAATGGACAGCCAAGACAAATGTCAGCAAGTTCAAATCCAAAGGCATACACCTCTGACTTTTAAAAAAAAATATGTGTGTATTCTTTGTAAATTATCACTTGCTTTACGGTGAAGGAAAACATCATGAGGAAACTCTCAATAGTAAAGGTATCCCATTGCCCAGCAGTGGGACAGTATATTATACAGGGTTGATATTATTATTATTATATACTATGCTTTGCTCATGCGCTCATTCACTCCAGCATTAATGACCTTTCCTGGTATTGAAGTTCTGCTAAGTATTTTATGGGATAAAAATACCCTGAATGTTAATCCACACACATTCAGTATGTCAAATTTCATCTAAATCCATTTAGGTGTGTTCTATTTGATACTAAGTTCCAACAAAAATCTTCATAAACGTCAGGACCCCTCAGGAGCAACATTATTAATAAGATTAATAATAAATTTTCAATGCATAATAATTTTAATATAATTTATATTACTTAATGTTGAAGTTGATATACTTAA> M. sexta-Relish F (MsRelF); HM363513.1 5′UTR (SEQ ID NO: 65)AGAGTACGTTCGATGGCAGTCTGTCGAACATTGGTAGTTTCCCGTTTGAGTGTTGTTTACTCCCTTTGAGGGATTAGTTTATTCTCCACGAATATAAACATCGGAAAATCAAAAACTAAGTTGATAAAAAGTTGTGTGCTCCGGTATAATTTTTTGGTATCAGTGACGGACAAAGGTGAT ATAAAA3′UTR (SEQ ID NO: 66)TATATTCATGAGAAGGGGACAACTAAGGATTGAATATCAGCAGAACTTGAGTTATGTTAATGAGGTATTTATATTAGCTTAATACTTAAAGGGGAAAATTCGATTAGCTTTTCAAATATACTTAAATTTTAGTTTTTGTCAAATATCGGTGTTTCCCATTTTGATATTTTTTTATCCATATTTTAATAATAAATATCTTGTCTTAGCAATTTTAATGGGTAATTATAAAGAAACTCACGCACCATAGTTGCACTAAACTACAAAATTACACAAAAAAAAAAAAAAAAAAAAAAAAAA> M. sexta-Chitin synthase 2 (MSCHS2); AY821560.1 5′UTR (SEQ ID NO: 67)ATTACGCTTTGAGCAGCCACTTTGTAAACACTAGCCTATTAACTCCGTCACTGCTTCGGCGATAACACTTCTGTATATCTGTTATTGTTTGTTAATTTTTGGCGCAAGGATATATTTAATATGTGCTAAGTTTGTGGCTAATTTATATTATGTTTCTACATTAAAGGTATGTATTAGTGATTTTTTGTAACTTATTTAATTACAAACATTATAACATATTTTATTAGAAAAGGGTTTTGTATCCTATGTAGCGCCTGTTTCACGATGCCTCTTGAAGTATACTGAAATGGGTACCCGTCGATAGTGTTTGCTTTAATAGGCAACGCATAGTGAAAACTTTGTACTTTAACCTTTTCAAAAGCATGAATAATTAAAAAAAAATATTAGAATAATGTGAAAAAGGTGTACAGTGTGATAAATATGGAGTATACAATATTCTTGTTCCAGTTATAAGATAAGGCACA 3′UTR (SEQ ID NO: 68)TATTTGGCCAAAATTGTGAACTGTAAACATGCAATAAAATGATGAACG> M. sexta-Beta-fructofuranosidase 1 (MsSuc1); GQ293363.1 5′UTR(SEQ ID NO: 69) TGAGAACAAAAACATTAGCTCCGCGTTTAAAA 3′UTR (SEQ ID NO: 70)TTATCGTGCTAAAGTAATAGTTATTGTTTCACATTATGTTCAAATAAAAAAGTAATTATTTATGTTCAAATAAAAAAGTAATTATTTAAGTGTCTTGTAAATCTGCGAATAAATATACTTAATGTATAAAAAAAAAAAAA > M. sexta-Sickie (MsSck); Msex2.03324RA(SEQ ID NO: 71)ATGACGACGTTGACTGCAAATAGGCAAGTGTCGGTGTTGCAGACGTCGATCCCGCTGCCGGCGTCGGCGGCGGCGGTGCGGCGCGCTCCGCCCGACAAACGACCGCTGCCCGCCACACCAGTACATGCAGGTGGCAAGAGTGGTCTTTCTGCGAGTTCCAGCCGCTCCACCAGTCCATTAGGACAGGGTGCAGTCAGTTTTATACCACGTGCGCCTAATTCATCATCTGGATCCCGGCCGAACTCGTCCCTCCTTGCGCCCAGTAGCAAAATACCCTCCACCGCAACACAAGGGCAACAGCATTCACAAACACCCACAGCCCAAAATGGAACACCCACAAAGCAGTCTATGCTTGACAAGTTAAAGCTATTCAATAAAGACAAAATTAGCAATGAAAAACAAAACAGCAAAAGCACTGCAGTATCAAAACGCACCAGTTCTTCCAGTGGTTTCTCATCGGCAAAGAGTGAGAGATCAGACTCGAGTTTAAGCCTTAATGAGTCTTCTAACATACCCAACACACATATCAAATCGTCTAATTTAATAGGACCTAAAACAATACGGCAACAAAGCGATACTTTATCAAAAGACAAATCTGGTAAAAATTTAAAACCTAAGTTAGTTAATTCTAAATCTCCTAGAGATTCTACAACTAACTTGAATAGGGCTAGTAGCAAAACAACTAATAATGATAAATCGAGGAATAGTCCAAAACTTCCTGCTAGGGATAAGGAATCGAAACTAGCCACACCTAAAACCATGAGTAACACAAAACTAAACCAAGTTGAAGATCAACCGAGGGGTTCCAAAACTAAAGTGGAATCGAAAATGGTAAAGCTTTCCGGTAGCCAAGTTAAGCTGAGTGAACAAAGATCCGATACTCCACAAGACTGTAAAGAAAATGTAACTCCTAATGCGAAAAATCATCAGAGTCACTCTCAAACACCATGTGGAGGACAGGGATTTGGAACTCCAAACCATACAGGAATACCTAAACCGACTGCCGCTGTCAAGGGAACTTTTAAAATATCAAAAGACGATAGACATGTTATACAGAAAAGTACAAATAGTCAATTAAGTCCTATACAAAGCAATTCTAGTTTAAATTCTACAAATAACATAAGTGCACTTCCATTCTGTAGAGAACAATCTAATTTAAGTAAGGATATAACTCAAAAGCAGACACTAGCCGTATCCCCGATGCCGGTTATGAGCACAGGGAATCAAAATCATAGTTCGCAAATGTCTGAAAGTTCACACTCCAATTCTACGCACAGTACTACAGGCCAACATTCGAACTCTAGCGATAGCAGCGTTATATACCGTCCTTCTAGCGAATCAGGTTCTGAAATGTCGAAGACTGCTAGTCACAGTGTGGCATCAAATAAACGGCTAGACATGAATACTACTTATATAAATGACGTTATAAACGAAGCGGAGATATCAGAAAAGGAGGCAGCACAAAGACGTCACGTCGATAATTCAATCCCCAAGCAATCGTTTGATCCCAATAAAACGTTAACCGAAATAAGTAGGAGGCTCGAAAGTGATAGTAGATCTAGCACACCGTCGCATTGTCGTGATAATTCTTTAGGCGAAGACGAGAATCCAATGATGAATGTTTTACCAATGAGACCGTTACTTCGCGGATACAACAGTCATTTAACCTTGCCCATGAGAACTTCAGGTTTGGCACAGAAAAATATAAGCGTTTATCCACACCACGCAAATACTGTAAAAGCAAACTTTGGACGGGAAAACATAGGTCTTCGCGATCGTATAAACTATGGGCCTGGTTTTTCGAATCCTGATTATTGTGATCTGGAAATTGCTTCGGGATACATGTCTGATGGTGATTGTTTGAGAAGAATTAATATCGGTGAGATGGAATGCGGGAGGAACAACGACATGATGGACGGCTATATGTCCGAGGGTGGTGCTTCACTCTACGGTCGTCGGATGAACTACCAGCAACCACAATTTCAACAAATGGACGAAAGACGAAGTGGTGGTCGCAATAGAGGCATGGAGGGTGGTAGTGGTGTAGTCTACAGAGTAGTGGGTCGCACACGCAGCAAAGCCGACTCCGGACAGCAGACCGAGCGCCAGCCTCAGCCTTCCCGGCAGGACACCACGTGGAAGAAGTACACTGACTCGCCCGGGAATCAGCCAGCACCCGCGCCTCCTAGTCCATCTCACTCGCGCAAGGGTGAGAGACGAACTGGCCATCATTCACCGCAGCACCACAAACGAGAAAAACTCACTGCAGCTCAGCAGCTTGGGATCGCACCTCATCCTCAGTATGCCGCTCCAGCTCAAGCAGCTAACCCTGCTGGTCAGCATTCCTCTAGAAACCCAGGCCCACAGCTCCAGTCTCCGAGTGGAGGGTCGCGTCCCTCCAGCGTCTCTAGCGGTGGTAATGGCAGTGCCTGCTCGTCGAAGGCTAAAGTTCCTCAAAATTTTGGATATGTGAAAAGACAAAACGGTGTACAGCAGCCGGCACCTCAGGCCAACGGTCCACCGCCGCAACATGGAGGCCACACTGGAAGAACCGCCCAGGTGTCTGCAGTGCCAAGAACTAAAGTCAAAGTTTCGGGAGGAACCCAGACATGCACACAGGATTTACAGATTCACAAAAATGGTATCGGACCTAAATCGTATTCTCTGGGCGGCACCGCGGCTGCCCAACTGTCAGCGTCAGTGCGAGAGAGACTGCTCGGGTCACAATCACTACCAAAACCTGGAACACACGAATTCGCAGCGTTATTCCATCACCACAGAATTGCACCCAGAGGCGGCATGAAGATCAGCGATGGCAGCCTTTCTGACACACAGACATACTCTGAAGTGAAATCAGACTATGGGATACCCTACGCGCCCTGGCTCAGGCATAGCAACACATACTCGGCGAGCGGGCGGTTGTCAGAGGGAGAGTCGATGGAGTCGCTCACGTCGCTGCACTCGGCGCAGGCGCAGGCGCACAACCACACCTCCTCCCCTAACTCCCACCGCAGCTCACTCACACACAATAAGCTCATCATGCACCGAGATGCACAGGGCTCCAGGCTTAACAGGAGCAACAGCATTAGATCGACTAAGTCAGAAAAATTATATCCGTCGATGCTTCAAAGGTCTTCAGAGAGCGATTATGAACCGTATTATTGTTTACCTGTGCAGTATGGACCAACAGGTCAAGGTCTAAACTATGGTGTGTCGGAGCCGCCGTCGCCGTCGCCGCGCTCGGCGCTGAGCCCGACGCACGCGCCCGGCAACGTCATGCACACGCCGCGCCACTCACACCACTACCCGAAAAAGAACGACGATGTGCACGGTTCGACGGCGTCGCTAGTGTCGACCGCCTCCTCGCTGGCTGCCGGCGCAGGCGCAGAAGAGAGGCACGCCCATGAGGTGCGAAAGCTGAGAAGAGAACTGGCCGATGCGAAAGAAAAAGTTCACACTTTAACAACGCAGTTAACAACCAACGCGCACGTGGTGTCCGCATTCGAGCAGAGTCTGTCGAATATGACGCAGCGCCTGCAGCAGCTCACCGCAACCGCCGAGAGAAAGGATTCGGAACTGACGGAGCTTCGTCAAACGATTGAGTTGCTGCGGAAACAGTCGATTCAAGCTGGTCTGACTACTGCTCACATGCAGTCCATGGGAATCCGTGCCGATGGCGTCAACGTCACTGGACAGCAGGACCCAACAACCCAAACGCAGCAGTCATCACCACAACGTAACGCACAAACGGGCAACGGTGCGATCACGCGGCATCTATCGACCGACAGCGTGTCGAGCATCAACAGCCTGAGCAGCGGCTCCTCAGCGCCTCACGACAAAAAACACAAGAAAAAAGGATGGCTACGCTCATCGTTCACGAAGGCATTCTCACGGAACGCGAAGATATCTAAAACAGCGAAGCACTCGTCCCTCGGGCAGCTGTCGTCACAGGACAGCTCGTCGGGCTCGCATCACTACGACGACCCGCACACGATACGAGAGGGCAGTAATGAGAACAGTCTAGAACATTCCCACGAGGCTCTTCCTGATACTAAGGAGAAGCCGGCGCCTGTCAAGCCTGAAGAACAAACCAAAGATAACAAAGAGGAATCTGTCTTGGTGGATGAGCTGAAGCGGCAACTGCGCGAGAAGGATCTCGTGTTGACCGACATCAGACTGGAGGCGCTCAGCTCCGCGCATCAGTTGGAGAGTCTCAAAGACACCGTTATAAAAATGAGAAACGAGATGTTGAACCTGAAGCAAAACAACGAGCGTTTGCAGCGGCTGGTGACGTCACGCTCGCTCGCCGGCAGCCAGAGCTCGCTCGGCACCGGCGGCTCCGCCGTCGAGGACCCGAGGCGGTTCAGCCTCGCGGACCAGGCCACCATGCACCAGGCGGCGATCGACATTCACGCCCAGCCTCTCGACCTCGACTTCAACTGCATGTCAGCCACACCAACCATCGACTTCTCGAAGAAAGGTTCACCCAAGTCCGGCATGGTGGAGCCCATATATGGGAATAAGGCTGCCTGCGAACTGAACGAGAACAATGAGACGCTGCTAGGAGCGTTGAATGGGGCCAGCGATTTGTTCTCCAACGGACTCAATGCTGGAGAGCGGCTGAGTGGAGATTACGATATAAACAGCGTGTTACCGCCGCCTAAAACACGCGAGCTTGCTATTGGTGAAAGCTACTCTGATATTGGTGTAGCAGACAGCCAGGGAGACACGACTGATGGAAAGAAAATAGCGATAGCTGTATATTTAGGTCAACCCGAAACATTCCAAAGATATTTCGAAGAGGTCCAAGACACATTGACGGAGTCCGAGTGCAGATTCTACGCGAAGCAATCGGCAAGCGCGTACAATCATTTCGAGAAACAAGCCAGTTTCGAATCGCCGAGAATGTCCACGAATCACAGTCCAGAAGTGGAGACACAAGACTACCCGCAAATAAATAAGTCGAACACGAATAGCCTTAAAAGCAACAAATCTACGCACAGTAGCTCGTATAAGAATGTTTATAATAGTGATTCGACAATAAACTGCAACGAGTACACTATAGCGTACACTTATATATCTGGCAAGACGACTTGGCAGAATTTAGATTATATAGTTAGGAAGTCCTTTAAGGACTACTTGTCTAGGATAGATTTGGGCACGAATCTCGGTCTGAACACTGATTCTATAACGTCGTACCATTTGGGTGAAGCGACGCGTGGGCCGGAGATCGGCTTCCCGGAGCTGTTGCCATGCGGGTACATCATAGGGACCGTGAACACGCTGTACATCTGCTTGCAAGGAGTCGGCAGTCTGGCGTTCGATAGCCTTATACCAAAAAATATTGTATATAGATACGTTTCTCTGCTATCGGAACACAGGCGAGTGATACTCTGTGGTCCCAGCGGCACCGGCAAGTCATACTTAGCGGCGAAACTCGCTGAATTTTACGTCCAGAAAACACAAAGGCGCGGCAATCCCGCCGAAGCTGTAGCTACATTCAACGTGGACAGAAAGTCGTGCAACGAGCTGCGCGCGTACCTGGCGAACATCGCGGAGCAGTGCGGCGCGGCGGCCGCGGGCGAGGAGGCGCCGCTGCCGTCCGTCGTGGTGCTCGACAACCTGCAGCACGCCTCCGCGCTCGGCGACGCCTTCGCGGGGCTGCTGCCGCCCGACAACAGGAACATGCCCGTTATTATCGGTACCATGTCCCAAGCGACGTGCAACACCACGAATCTCCAACTACATCACAACTTCAGATGGCTCCTCACCGCTAACCACATGGAACCCGTCAAAGGATTCTTAGCTAGGTATCTCCGAAGAAAGTTATTCTCGCTGGAGCTGCGGCTGGGTCGGCGCGAGCCGGCGCTGGCGGCGGTGCTGGAGTGGCTGCCGGGCGTGTGGGCCGCGCTCAACGCCTTCCTCGAGGCGCACTCCTCCAGCGACGTCACCGTCGGGCCGCGGCTCTTCCTCGCCTGCCCCATGGACTTGGAGGCCAGCCAGGCATGGTTCGCAGACGTGTGGAACTACAGCATAGTGCCGTACGCTTCGGAGGCGGTGCGCGAGGGCATCGCGCTGTACGGCAGGCGACGACACGCCGCCGTCGACCCGCTGCAGCACATCAAGTCTACATACCCCTGGAGAGAACCCAATCACTCGCATACTTTGAGACCCATAACAGTTGATGATGTTGGCATCGAAGAGTCAAGCCAAGACTCCGCCGTGAACAACAATCAAGATCCTCTGTTGAACATGCTGATGCGGCTACAAGAAGCGGCGAACTACAGCGGAAACCAAAGCCAGGACTCTGACAACGCCAGCATGGACTCGAACCTCACACACGACAGCTCTGTAGGGAAC GAGCTTTAA> M. sexta-Akirin (MsAki); Msex2.12479-RA (SEQ ID NO: 72)ATGGCGTGTGCTACACTTAAAAGAAATTTGGATTGGGAATCCATGGCGCAATTGCCTGCTAAAAGGCGAAGATGTTCGCCATTTGCTGCAAGTTCTAGCACAAGTCCTGGATTTAAAGTGTCTGAAACCAAGCCATCTACATTCGGAGAGGCCGTTAGTGCACCTGTGAAAATGACCCCAGAGCGCATGGCTCAAGAGATCTGCGACGAGATCAAGCGGCTGCAGCGGCGCCGGCAACTGCGGCTGGCTGGCAGCTCCGCCGCTTCGTGCTCATCGTCGAGCGGCAGCGAGGGCGACTGCTCGCCGCCACATCGCTCCTCGCACACTTCGCACAAGATGCACAACCGTGCGCTCTTCACTTTCAAACAGGTGCGCATGATCTGCGAGCGGATGCTGCGCGAGCAGGAGGTGGCTCTGCGCGCGGAGTACGAGTCGGCGCTCAGCACCAAGCTCGCCGAGCAGTACGAGGCGTTCGTGCGGTTCAACCTTGATCAGGTGCAGCGCAGACCCCCGCCCAGCACGTGCATGCCCCTCGGCATGGACGCCGAGCATCACATGCACCAGGACCTCGTACCTAGCTATCTGTCCTAA> M. sexta-Cactus (MsCac); Msex2.02793-RA (SEQ ID NO: 73)ATGAGTGCCAAAAAAGGATATGAAACGAAGATTGTCGAGGAAGAAAACATGGATTCCGGAATTGTGTCTGGTGAATTGGAATCTTATGAGATTTCGGGTGAAGTGGATTCGGGCGTGATTGATTGTGATAAGAAATACGAAGGGGTTCCAAGTGAGGTGTTGGAATTGACGGACAAGTTCAAAAGTGTAAATGTGAGAGAGAAGAGCTGTCCTGATGTTCCACCACTGGCGGACCTGTTCCACCCTGACAACGACGGAGATACACAACTACACATTGCATCGGTACACGGCTGCGAGAAATCAGTGAGCACGATCATCAGGGTGTGCCCTGACAAGGAGTGGCTGGACCTGCCCAACGACTACGGCCACACGCCCCTCCACCTCGCGGTGATGAGCGGCAATGCCGTGGTGACAAGGATGCTGGTGATAGCCGGCGCTTCGCTCGCTATTCGCGACTTCATGGGAGAGACGCCCTTACACAAGGCGACCGCAGCGCGAAACCAGGAGTGTCTCAAAGCCCTGCTTGCCCCTGTACCGGAACAGCCCAATAGGAAATTGTCTTCAATACTCGACCAGAGGAACTATAACGGTCAATGTTGTGTCCACCTGGCGGCGTCAATTGGAAGCGTAGAGACGCTACAGACCCTGGTCTACTACGGAGCCGATATCAATGCCAGGGAGAACCTGGCGGGCTGGACGGCGCTGCACATCGCGGCGCGGCGCGGCGACGTGCGCGTGGTGCAGTTCCTGCGGTCGCGCTGCGCCGGCGCGGCGACGCGGCCGCGGGACTACGCCGGCCGCACGCCGCGCCGCCTCGCGCGCCGCACCAAGGCCGCCGCCGCCTTCGACGACAAGGACGACAGCGACTCCGACTCCGACTCGGACGATGATGATATGTACGACAGTGATAGCGAGACGTTGTTCGAAAAACTCCGCGAGAGCCTGAGCACGTCGATCAAC GTCGCCTGA> M. sexta-Gloverin (MsGLV); GI110649240 (SEQ ID NO: 74)CCACGACAACCACTGATGAAGTTATTTTTTATAGCAATTCTTTTCGCTGCCATCGTCGCTTGCGCGTGCGCTCAAGTGTCGATGCCCCCGCAATACGCTCAGATATATCCAGAATATTACAAGTACTCCAAACAAGTCCGCCATCCCAGAGACGTGACCTGGGACAAGCAAGTCGGCAACAATGGGAAGGTCTTCGGAACTCTGGGACAGAATGACCAGGGTCTTTTCGGTAAAGGAGGCTATCAACACCAATTCTTCGATGATCACCGCGGCAAACTGACAGGACAGGGTTACGGGTCCAGGGTCCTCGGACCTTACGGAGACAGCACCAACTTCGGCGGCCGGCTTGACTGGGCCAACAAGAATGCTAACGCTGCTCTTGATGTGACCAAGAGCATTGGCGGTAGGACTGGGCTGACTGCCAGTGGATCAGGCGTGTGGCAACTTGGGAAGAACACGGATTTATCTGCGGGAGGCACTCTGTCTCAGACGCTTGGACATGGGAAGCCTGATGTCGGCTTCCAAGGTCTCTTCCAGCAT AGATGGTGA> M. sexta- Beta-1 tubulin (MsβTub); AF030547 (SEQ ID NO: 75)ATGAGGGAAATCGTGCACATCCAGGCTGGCCAATGCGGCAACCAGATCGGAGCTAAGTTCTGGGAGATCATCTCTGACGAGCATGGCATCGACCCCACCGGCGCTTACCATGGCGACTCGGACCTGCAGCTGGAGCGCATCAACGTGTACTACAATGAGGCCTCCGGCGGCAAGTACGTGCCGCGCGCCATCCTCGTGGACCTCGAGCCCGGCACCATGGACTCTGTCCGCTCCGGACCTTTCGGACAGATCTTCCGCCCGGACAACTTCGTCTTCGGACAGTCCGGCGCCGGTAACAACTGGGCCAAGGGACACTACACAGAGGGCGCCGAGCTTGTCGACTCGGTCTTAGACGTCGTACGTAAGGAAGCAGAATCATGCGACTGCCTCCAGGGATTCCAACTCACACACTCGCTCGGCGGCGGTACCGGTTCCGGAATGGGCACCCTCCTTATCTCCAAAATCAGGGAAGAATACCCCGACAGAATTATGAACACATATTCAGTTGTACCATCACCCAAAGTGTCTGATACAGTAGTAGAACCTTACAATGCAACACTGTCAGTCCACCAACTCGTAGAAAACACCGACGAAACCTACTGTATCGACAATGAGGCTCTCTATGACATCTGCTTCCGCACGCTCAAACTTTCCACACCCACATATGGCGACCTTAACCACCTGGTGTCGCTCACAATGTCCGGCGTGACCACCTGCCTCAGGTTCCCCGGTCAGCTGAATGCGGATCTCCGCAAGCTGGCGGTGAACATGGTGCCCTTCCCGCGTCTGCACTTCTTCATGCCGGGCTTCGCTCCGCTCACGTCGCGCGGCAGCCAGCAGTACCGCGCCCTCACCGTGCCCGAACTCACCCAGCAGATGTTCGACGCTAAGAACATGATGGCGGCGTGCGACCCGCGTCACGGCCGCTACCTCACCGTCGCCGCCATCTTCCGTGGTCGCATGTCCATGAAGGAGGTCGACGAGCAGATGCTCAACATCCAGAACAAGAACTCGTCGTACTTCGTTGAATGGATCCCCAACAACGTGAAGACCGCCGTGTGCGACATCCCGCCCCGTGGTCTCAAGATGTCGGCCACTTTCATCGGCAACTCCACCGCTATCCAGGAGCTGTTCAAGCGCATCTCTGAACAGTTCACCGCTATGTTCAGGCGCAAGGCTTTCTTGCATTGGTACACCGGCGAGGGCATGGACGAGATGGAGTTCACCGAGGCCGAGAGCAACATGAACGACCTGGTGTCCGAGTACCAACAGTACCAGGAGGCCACCGCCGACGAGGACGCCGAGTTCGACGAGGAGCAAGAGCAGGAGATCGAGGACAACTAG> P. xylostella- Peptidoglycan recognition protein 2 (PxPGRP2);ACB32179.1 (SEQ ID NO: 76)ATGACGTTGTCTTTTGGCGTGTTTCTGCTGATATCTTCAGTGTTTTGTTGTTGTGCTCATGCAGGGTGTGGCGTGGTGACCAGACAGCAGTGGGATGGGCTGGACCCGATACAGTTGGAGTACCTGCCCCGGCCCCTGGGGCTGGTGGTGGTCCAGCACACCGCCACCCCCGCGTGTGACACTGACGCCGCGTGTGTGGAGCTGGTGCAGAACATACAGACCAATCATATGGATGTGCTGAAGTTTTGGGATATTGGACCGAACTTCCTGATTGGTGGGAACGGCAAGGTGTACGAGGGCCCTGGTTGGCTGCACGTCGGCGCCCACACTTACGGCTACAACAGGAAGTCTATCGGGATCTCTTTCATTAGGAATTTTAATGCTAAGACCCCAACAAAAGCAGCGTTGAATGCGGCTGAAGCATTGCTGAAGTGTGGAGTGAGAGAAGGACACCTGTCTCACTCATACGCAGTGGTCGGCCATAGACAACTGATCGCAACAGAGAGCCCAGGCAGGAAACTGTACCAAATCATCAGGCGCTGGCCAAACTACCTCGAGGATATTGATAAGATTAAAAACAACAAGTAG> P. xylostella- Immune Deficiency Protein (PxIMD); Px003008(SEQ ID NO: 77)ATGTCTATCCTAAAATCAAAGTTATTCGAAACTATTGCAAAAAGTTTCAAGTCTGATGCAGTCCCGAAGCCACCTAGAGAACCGGTAGAGACTACAGAGACATCACAAAATAATACCGAAAATCAACCTTACAATGTCGAAGAAGAGGAAATACCCGAACCAGAAAAGCCTAAGAAAGAAAAAAAGAATCCCAAGCCTACCAAAAAAACTTTCTTTAATCGTGACAAAACTAACAAACACGACGATACCCGCAAACATACAAAATCCGGAAAGGACCAGACATCAATTAATACTCAAGGTAACTTGAAAATTATACTTCCTGTAACTTAAACGGCCCGTTGACCCCAGTTTACCTTTCGCCTTTCTTGATATATTTTTGTAATCCAGCCTTACTTTGGTAATACATACTTGCCCCACTTGTATTTAGTTAATGGTGGCACTAGCTAGATAGTAATGTTAAATGATGATAAGCAGTAGTGATTCATCATTCAAATGTATCATTGTCCTTTAATGTTAAGCGCAAATAGATTTTCATTGTTCTCCCATGTGCTTCATGTTTTATGTATTTATAGGTAGGTACTTAATGTTTTATAAATATTTTTTTGTTAATTGGGAATCCCCAGTCCCCATTGTCTGGACCAGTTTATATATAATTGAACTAACAAGAGTGTGCTTTAAATACTATTCTCTGCAATTATGATAATTAAACAACATGAATTTCTCTTCACTTCCCTTCTCTTATTTAAATAATATTGTAGGAAACTGTAATAACTAATACAAGATTATAAATTTCATTCTAGCAACTGGTGATGTAATCCATGTGGTAAATTCCAAAGATGTGCAGGTCGGCCATCAGTATGTGTACAACATGGGAACTCCCGGAGCTAACTCACAGAAGAATAACCCATTTGATGATGAAGAAACAGTAGAAAAGACAAATCTAATAACTCTGGTCATGGAAGCAAAAATTATGGTAATAACACATTTTTAACTAGGCATAAGGTCATAATTTAGCCAGAATCATCAGCTTGTCCTGTGGCTCTTGTTGAGCTGGTGGAAAGAATACATAGGTAATGAATATTTTGATCAATACTCATTGCAAAAATCACAATAATGCCATTGAAAATCTATAACATGTTCCTTAAGTATCACTTATCATCAATCCAATTAAGTCATCACACAGATCAATCGGTTAGTTCTGTTTATTTACTTCTTTCAGCTGGAACATGAATACATGGACTATGTCTCGAAGAACCTCGGCAGGAACTGGCACAGCTTCTTCAGAACGCTCGGCTTCACGCGGGGGCGCATCGAGACTGTGGAACTGGATGAGGGCAGAAATGGTGTTGCAGAGGTATGAAAAAAACATACATGTTAATTTGTGTTGTTTGGCTGATGTGGCAACTAAGTTCGTAACTGCTATGATCAACTGTTTGTGTCATAGGTATTTTTTTTGCCCTTCCACACATCTGAGGTAACAAGGACACCTCCTGCACTCAAAACACAACTGCTATGTCCTACTGATGAGTCCTAGGAAACTCAGAAACCATCTGTTTCCCCCATTCTCAATTCCATAAAGCTAAAAAGTGGTAGTTACACAATTCACAATTCATAAACATGCTTTGTCATAGTTAGAAAAGCAGCTCAGCTATGAGGCATCCACTCCACAGTCCACTCACGAAAGCCCTCTTTAAAAACATAAAATCATCATCATCAGCCCTCAATTGCCCACTGTTGCATATAGGCCTTCTTTTGATTATGCCAAGTTTTTCGTTTCTCTAGTCCACAGACTTGACATAGTTGTCACAAATTTATTCTACGTACTTGTATTTCCAGGTGCGCTACAAGTTGCTCCTGGAGTGGGCCCGCACCGACGAGGACCCCACGCTGGGGCGGCTCGCCACGCGGCTGTGGGACGAGGGAGAGCGGCAGACCGTCAAGGAACTCGCCATCTTGTATAATAATAATTTCAAGCAACAA TGTTGA> P. xylostella- Relish (PxRel); Px002858 (SEQ ID NO: 78)ATGGTAGTGGTGAAAAGCCAATTAAAAATGCAAAGTGACCAGGACACGGACTCGTCCACTGCCGGTGCTTCGCCGCGCAGCTTCTACATCGAGTCGCCGCACAGCTCGCCGGGACAACAAGTGCCTTATTTAACTAATTACATGACAGTACTGTCCTGTGCAGATAATAACTTAATGGATACAGGAAGCAATGGACCATTCCTGAGCATCACGGAGCAGCCATGTGACCACTTCAGGTTCCGCTACAAGAGCGAGATGGTCGGCACCCACGGCTGCATCGTCGGCAAGACCAGCGCCAGCAACCGCACCAAGACATACCCTTCTGTTGTTCTGCTCAACTACAAAGGCCGCGCCACCATCAAGTGCAGCCTCGCGCAGCACAACAACCGCAAGCAGCACCCGCACCAGCTGGTCGAGGATGACCAGGAGCGCGACCTGAGCGCCGAGGTCAACCCCGAGAAGGGCTATGAAGTTGGATTTCGTGGCATGGGCATAATACATACAGCGAAGAAAGATGTTCCGGCACTCCTATACAAGAAATTGAGTGAGAGACTGCCACATTTCAATGCCCGTGAGCTGAAGGCCCAGTGTGAGAACGAGGCGCGCAGTATAAACCTCAACATCGTGCGCCTCAAGTTCAGTGCGCACAATGTCGACACGGACGAGGAGATATGCGCTCCGGTGTTCTCGGAACCTATCCACAACATGAAAAGCGCCGCGACGAACGACCTGAAGATCTGCCGCATGAGCCGCACGTCGGGGCGCCCGCGCGGCGGCGACGACGTCTACCTACTCACCGAGAAGGTTAACAAAAAGAACATCGACATTCGCTTCGTGCAACTGGAGCGCGGCGAGGTGTGCTGGACCGGCAAGGCCAGGTTCCTCATGAGCGACGTGCACCACCAGTACGCTATTGTGATCAGAACACCAGCATACAAGAACCCCGAGATTACGTCTGACGTAAAAGTGTACGTAGAACTATTCCGCCCGTCCGATGGCCGCTCCAGCGAACGCATAGAGTTCACGTACAAGGCAGAAGAAGTCTACAAGCAAAGCAAGAAACGGAAGGCCAACTCTTACTCCTCTATCGGAAGTTCATCTAGCGGTAATTCTATCAAAAGCGTCAGTGATCTTCCAGCAACTGTTATTATGGCCAATGAAATGAATGCGGCTAACAACAACTTTAGTAAAATCTCTTCAATGCTGAGCCCAAACAATATACCAGAAATACCGACACAAACGACTGTGGGTCTATCAGACGCTCTCTACGACATAACAGTGACAGAAGACCACCAAATGCACATCAGTCCCATGCTATGCCAACCAGTGGAAGAGTATCCCCTAAAGCTTAACTCGCAGGATATCATACAAGTGAATTTGAACTCTAAGGACATCGACCAACTGCTCAAAGTCAACAGTGTGCCTGATACCGATAAAGACTTTGCTGACTTCAATTTTAGTGACTACTACAAGGCACTCGATAGCAACTTTTTAGCTGATGGTGGTGGTGATAGCTTCAGTCAGTGTATCTTCAACTCTATGCAACTGAGGCCTGACTCTGGGAGAGGCACG > P. xylostella- Toll receptor (PxToll2); Px006338(SEQ ID NO: 79)ATGCCTAAAGTAATAATCGCCAGTTTTGCTTATATAGTAGGTGTGTTTGTCCTGTGTGCTGGGCTAGAAACAAGTCCAACCTGTTCCAGCATCGAAGGACCTACTCCAGGTGAATTCATAATACAAAGAGGTATCGTACCGGACAATGATTCAGCCACCACAGTAAGCTTCCGAGGCTGCCGAATATCTGACATTCAGCCGAGAGCATTCCATGGGCTACCTTCCCTGCAATACATAGACTTATCAAGAAATAGCATTAAAAACCTGAAACTTGGCATTCTTAATGACGTCACTAGACTCACTCATCTGAACTTGTCTTATAACTTCATCAGCGATTTAGAAGAGAGTTTGTTCAACCAATCGTCAAGGTTGGAGGTGTTGGATCTCCGGTGGAATAAGATTGAAGTTATGAAAGTGGGCGTTTTCAGTCCATTGAAGAGATTGAAGTATTTGGATCTGTCCGACAACGAAATAGTTGGGGCCAGCCTGAGCCCCGCTATGTTTGATTCCTGTAAAGCTCTATCCACTATCAATTTTTCAAGAAATGATATGTCTGGTGCTCCCACTGATTTGCTTCGAGCTGTGGAGGTACTGGACACACTGAAACTGGATGGGTGCTTTTTGAAACAAGTTCCGGAATTTGCTACGAGGAGCAATACCGGCACAATGAAGAAACTTATTTTATCATCGAACCAAGTGAGCACTGTGAAACTTACTACGTTCATCAGTTTAACAAACCTGGAGGAACTAGATTTGAGTTCAAATGTAATTTCAGAATTGCATGAAGACGTATTCAAGCCATTAAAAAACTTGAAAATTATTATTTTACGCTCGAACCGACTGGAAAAAATTCCTGATAAGTTGTTTTATAATATGTTACGATTAAGGAAGGTAGATTTATCTTTTAATTCTTTGATGATAATACCTGTGAATGCCTTTCGTTTTACGACGATAGAAATGTTGAATATATCGCATAATAAGTTCACATATTTAGTCGACAACTTTTGTTTGGAACTTAGAAACTCGGGAGTGAAACTGAAAAAGTTTTACTTCAACAGTAATCCCTGGCAGTGTCCTTGCTTGAGGGATTTATTAAAGGAAATGAAGACGTATAGAATATCGTACAATAATGCTAAATACGATGGTAAAAATGCAGTTTGTATTTCAGGAGACATTATTAATACTTGCTTGAGACAACCTGATGTCAATGAACACTTCAATGATTTGTACTATTCTGACTAA> P. xylostella- Cactus (PxCac); Px016665 (SEQ ID NO: 80)ATGAGTTTCAAGAAGGATTTCGACACCTCAAAGAAGATCCAGGAGGATGAAAACACAGACTCTGGGTTCCTATCTGGGCCGATAAGTGAGCAGCTGACCTCGGAAGATTGTGATTTAGCGGAGGAAAGTGAGCGTGCTCGCAGCAGGCTTAGTGAGGAAGATCCTGAGCCTGAGCTGCAGTTGGACAGTGGGCTGGACCTCTCGGAGTGTCTGTCGAGTGTTAAGCTTAGTGATAGTGCAGTGTACACACCCACCTCGCAGACCACCCCCACAGTCACTATAGGTGATGAGAAAACTCATGACATCCCACCCCTCGCCATCCTGTTCCAGCAGGATGACGATGGAGACACACAACTACACATTGCAGCGGTACATGGGTGCGAAAAATCAGTAGGAACATTAGTAAGAGTTTGCCCTGACAAAGATTGGCTAAATGTACCAAATGACTTTGGACAGACCGCCTTACACTTAGCAGCCATGAGTGGGCATGCAGTAGTCACACGCATGCTGGTGATGGCCGGTGCATCTCTTGGCATTCGAGACCTTGTTGGCAACACACCTTTACATGTGGCAGCCGCAGCGGGCTACGTCGGCTGTCTCCAAGCTTTACTGGCTCCTGCTCCAGAACAACAGCAGAGAAGGCTAGCATCCACGTTGAACCAGAAAAATTACAATGGTCAAACGTGCGTCCATGTGGCTGCGATGGCCGGCCACGTCGACGCGCTGCAGACATTGGTCTATTACGGAGCTAACATCAATGCTGCGGAGGGTCTATGCGGGTGGACACCTCTACACGTAGCGGCGGCGCGAGGCGACGTCGACACGGCTCGCTACTTGCTCGAGAAGTGCGCTGGCGTCGATCCCTCTGCCCTGGACTACGCCGGTCGTACGGCCAGGAAACTGGCGTTGAAGAATAAAGCGGCCGCCCTGTTTGACGGCAGTGAGGGCAGCGAGGAGGAGGATAGTGACAGTGAGGATGAGATGCTTCTGGAAAGCGACCAGAGTCTGTTCGACCGGATCCGTGACGGTATGAACGCCATCAACGTCGCCTGA> P. xylostella- Dorsal (PxDor); Px000110 (SEQ ID NO: 81)ATGAACGCGCCCGCCGACTCCGCCGTGGTGACGTTCACCAACCTGGGCATCCAGTGCGTGAAGCGGAGAGACATCGAGGACGCCCTGGCTGTGAGAGAGGAGATGCGAGTTGACCCCTTCAAGACCGGATTCAGCCACAAGAACTCCCCGCAAAGCATCGACCTGAACGCCGTCCGACTCTGCTTCCAAGTGTTCCTGCCGGACGAGCGATCCGGCAAGATCCGCCACGCGCTGCCGCCGGTCGTGTCCGATGTCATCTATGACAAGAAGGCCATGAGTGACCTGGTTATCACGAGGCTGAGTCATTGTTCTGCGCCCGCGCAGGGCGGCAAGCAAGTTATATTGCTGTGTGAGAAGGTGGCCCGCGAAGACATAACCGTAACCTTCTTCGAGAAGTCCGGCGAGCGCGTGACGTGGCAGGCGGACGCGGCGGACGTGTTCGTGCACAAGCAGGTGGCCATCTGCTTCACCACGCCGCCTTACCGCGACCCGCATGTGCAGGACCATGTGCAGGCGTACATCCAGCTGCGTCGTCCGACGGACAACGCGACGAGCGAGCCGCTCCCCTTCGAGCTGCTCCCGTCCAGCGCAGATCCGAATTATCTGAAGCGAAAGCGACAGAAACCGATACAGAACTTCAGTCGGTACTTACAGCCGATCGATAGCGACATGAAGCAGCAGCTGCCGGACTATTTCCAGGACAACATGGCGCTGTCCAGCATCCCCTCCGTGAAGCTGGAGCCCCGAGATAAGACTCCTCCTCACAACATGAGCAGCCCGCCGCTGCTGTTCCCCCCCGCGCACGCCGCGCCCGCACACCATGACCCCTACGCGTGGAACATGCAACTAGACAACATGCAGTCGGGTCTGACGGCGCCCGGGCCGAGCCGCCTGCCGCAGTACAGCCAGGACATGGCCTGGACCAACCAGATGGGCCACGTGTCCCCTATGCACCAGGCCATGTCCCCTAACATGGGTCACGTCTCCCCCATGCATCAAGCCATGTCCCCAAATATGGGCCATGTCTCTCCCATGCACCAAGCTATGTCTCCAAATATGGTCCAGTCACCTATGGGTCATGTGTCCCCTAACATGGGCCATGTATCCCCTAATATGGGTCATGTGTCCCCTAATTTGGGTCATGTGTCACCTAACCTGTGCCAGCAACCAATGGCTCCTATGGCGCAGCAGCTGATGGACCCGTCCCCCAGCGACCCACCCTCCATCACGGGGCTGCTGATGGATCGCCCGGACCAGCCCTACTCCGGGGAGCTGTCTGGACTCTCCGCCCTGCTGGCTGAGGCAGCCCCCGCAGAGATGCTCAGCGATAGCCTCAACAGACTGTCTACGGGGGACTTGTTGAGACAAGTTGATATGTGA> P. xylostella- Hemolin (PxHem); ACN69054.1 (SEQ ID NO: 82)ATGACTTTAATTTTCAAGAGTGTTTTATTTTTGGGCTTAATATTGACTACTTTTATTGTTTCCGCTCAGCCTGTGAAACAAGATGGCGGCTCAGCACACGAAGAAATATTGTTCCGTGAGCACGGCCAGCCGGTGGTGTTGACCTGCGCGCGCGCAGACGACCCCAACCAAGGTGGCATTAGAACGTGGTTGAGAAACGGGACGCCATTAGAAGACGGCAAAATGTCTCCCGAAATAAAATTCCTCGACGACAAATCACTCTGGTGTTGCAGCCCTCACCAGCCGTGGAAGGGGTCTACCAATGCTTCACCGAAACCCATAAGGGCATTGCAACCTCCCCGAAATTCAGCGTGAAACAGACTTATCTCAAAGCTCCAGAGACTACGCCTTCAGTCAATATCAAACCAGCAAAAGGCCTTCCCTTTAGCTTGGACTGTGACGTCCCTGAAGGATATCCGAAGCCTGAAGTGCAATGGTTCCTACAACACGGGAAAGATCACACCCTGATTGAGGCAATTATCAATAAACGGATCACACAGGCTCCGAACGGAGCTCTTTACTTCTCAAATGCTACAACCGAAGATGTGAATGTGGGAGACTTTAGATACGTCTGTATGGCGAGGAATGATGCGGTAGACTTACCAGTGGTGGTGTCGGAAGCTGTCATCACAGGTCTGAGCAGCGAGGGTGGTAAGGGTAGATTGGTGGAGCAGTACGTCAGTAAAGAAGTTAGGGCGGTTGCGGGGGAGACCACAGCGCTATTTTGCATTTTCGGTGGCACCCCACTAGCCCACCCAGACTGGACGAAAGACGGCAAGAATGTGAACGGGGCGCCCGGCGACCGAGTGACCCGACACAACAGGAGCTCAGGAAGACGACTCATCATCAAGAACACCACTCTAGAAGATGCTGGAACTTACCAGTGCGCCGTTGACAACGGCGTTGGGACTGAAATGCGTTCTGTCAAGGTTACTGTTGAAGCGAAACCTTCAATAACGATTGTGAATGAAGTAGCAGCGAAGCTTGGAGAAGAAGTCAAGATTTGCGAAGCGACCGGAGTCCCAACGCCAAAACTAACGATAACTCACAACGCTAAACCGTTGGTTGCGTCAAATAACGTTGTTATAACCAACGATGGAGTTGTTATAAAGAATATTCAGGCTATTGATCGAGGGTATTATGGGTGTGATGCTGTCAACGAGCTAGGAAGCGAATTTCGTGAAACTTATCTAAGTATTGCCTGA> P. xylostella- Sph-3 (PxSph3); XP_004922188.1 (SEQ ID NO: 83)ATGCAGCTTAATTTCTGTATCAATGTGATCGCGACCATATTGTTGATTGTGACTGGCGGGGATTCCCAAAAACGAGTGGGTGACATTTGCATTGATCAATACACGAACACGTACGGAAGGTGTGTTTTCTCGGATCGATGTCCATCAGCTTTACGTAATTATCAACAGAACGGCATTCGGCCATCAATATGCACTTACAACTTCGACAATGCACTGGTGTGTTGTACTGAACGCGGAAATATTCTTCAAACGGCGAGGCCCCCGCCACCGCCTGATCAGGAAGACAGATTTCAGTCCTCTGCTGGAAACAACAACAACAACAATAAACCTAACATTAGAGTTAGCGAAAGAAAATGCCGCGAGTACAGCAAATCAGTAACGTTCACGGTGAGCTTCAGCTCGCTGCTGCCGGAGCCCGAGTTGCAGTCCATCTCGCGGCCGCGCTGCAGCCGGAGCGGCGTGGGGCTCGTGCTCGGCGGCCGGGACGCCGCGCCGGAGGAGTTCCCGCACATGGCAGCGATCGGCTTCGCATCAGCGGAAGGCTACGACTTCAAGTGCGGGGGGTCCCTCATCAGCGCGCGCTGGCCGCTGACCGCGCCCTGCGCGCGCGCCCGCGCCTCCAGCCGGCCCGTGGTGGCGCGCTTAGGAGATAGGAATATCAACCCGAAAGCGCAGGACGACGCCACGCCTGTCGACGTGCCAATCCGGAACATCATCGTGGACGTGGACCTCAGCAACAGCATCCGCCCCGCGTGCCTGTGGCCCGGCGGACCCTTCCACGAGGATAAGGCTATAGCTACGGGCTGGGGGGTGGTGAACCAACGCACGCAAGAGAAAGCGGACCTCCTCCAGAAGGTCTCGCTCACTCTGCTCGAGAACTCATACTGCGACCGTCTGCTGAGGAACAACCGCAACCGACACTGGCAGGGCTTCCGCGACTCGCAGTTGTGCGCCGGCGAGGTGCGCGGCGGCATGGACACGTGTCAGGGCGACTCCGGCGCACCGCTCCAGATCGTGTCCAAGGAGAACCAGTGCATCTACCACCTCATCGGCCTGACCTCCTTCGGCTACAAGTGCGCGGAGCAGAACAAGCCGTCGGTCTACACCAGGGTGTCGACTTACGTGGACTGGATAGAGTCTGTGGTGTGGCCGGAGGAGTATGCGGCTTGGGCGGCGGGGAGGAGTAAATAA> P. xylostella- Transferrin (PxTrs); BAF36848.1 (SEQ ID NO: 84)ATGATAGTGAAAATAGCCATTTTGGTGATAGCAATAACGTTCAACGATGTGTCTGCGAAAACTTCGTACAAGATCTGCGTACCGTCTCAGTTCATGAAGGCATGTGAACAAATGCTTGAAGTGGAAACGAAGAGCAAAGCGATACTGGAATGTTTGCCGGCCAGAGATCGAGTGGAATGCCTGACCCTGGTGCAGCAACGGCAGGCGGACCTCGTCCCAGTGGACCCTGAAGACATGTACGTGGCGAGTAAGCTGCCCAACCAGGACTTTGTGCTTTTCCAGGAGTTCCGGACCGATGAAGAGCCGGATGCGGAGTTCCGTTACGAGGCCGTCATAGTTGTTCACAAGGACCTTCCAGTTACCAACTTGGACCAGCTTAAGGGCTTGAAGTCATGCCATACTGGAATCAATAGAAATGTGGGGTACAAGATACCACTAACGATGCTGATGAAGCGCTCCGTGTTCCCTGCGATGACAGACCGCAGCATCTCTCCTAAAGAGAACGAGCTGAAGGCTCTCTCGACGTTCTTCAGCAAGTCCTGCATCGTCGGCCAGTGGTCGCCTGACCCGAAGACCAACACTTTCTGGAAGTCCCAATCCAGCAAGCTATGCTCCATGTGCGAGGACCCTGCCAAGTGCGACTACCCCGACAACTACAGCGGCTACGAGGGCGCGCTGCGCTGCCTGGCGCACAACGGCGGCGACGTGGCCTTCACTAAGGTCATCTATGTGCGGAAGTTCTTTGGGCTCCCAGTAGGCACAAGCCCGGCGACTCCTTCTTCTGAGAACCCGGACAACTTCGCGTACCTTTGCGCGGACGGGTCCAAGGTCCCTATCAGAGGAAAGGCATGTTCTTGGGCCGCAAGACCGTGGCAGGGGTTGTTGGGACATCAGGACGTTCTGGCCAAATTGTCGCCTTTGAGGGAGAAGATTAAGCAGCTGTCTAGAGCTGGAGCAGAATCGAAGCCGGAGTGGTTCACCAACGTTCTAGGCCTCTCTGAAAAGATCCACTTGGTCGCCGACAACATTCCCATTCGTCCCGTCGACTATCTGCAGAAGGCCAACTACACTGAGGTCATCGAGAGAGGGCACGGCCCGCCTGAACCTGTTGTGAGACTCTGCGTGACGAGCTCGGTGGCGCTGGCGAAATGCCGCGCCATGTCCGTGTTCGCCTTCAGTAGAGACATCCGCCCCCGGCTGGACTGTGTGCAAGAGGCTTCGGAAAGCGATTGCTTGAAAAGTGTCCAAGACAATGGCTCAGACCTGGCGTCAGTAGACGACATGCGGGTAGCGTCAGCATCCAACAAGTACAACCTACATCCAGTATTCCACGAGGTATATGGAGTCAGCAAGACCCCTAACTATGCGGTAGCTGTCGTCAAGAAGAATACTCAGTATGGAAAGATTGAGGATTTGAGGGGAAACGGTCCTGTCACAATCCTTTATGGAAGCTTCAGTGGCTTTGATGCCCCTCTGTACTACCTTATTAATAAGAAAATCATAGGCACTGAACAGTGCCTGAAAAAGCTTGGAGAATTTTTCGCAGCCGGATCTTGCTTACCTGGAGTAGGCAAATTAGAGAACAACCCTACAGGAGATAATGTCGATAATCTGAAGAAACAATGTTCTGGAGACAACAGCCCAATAAAATGCTTACAAGAAGACAAAGGAGACATAGCATTTGTGTCAAGTGCTGACCTGAAAAACCTGGATGCCTCTCAATATGAGCTGCTCTGTCTAAACAGAGAGAACGGTGGGCGAGACTCAATAACCAACTACGCTACATGCAACATTGCCATGGCCCCATCCCGAACCTGGCTCTCAGCTAAAGACTTCCTGTCCGATGTGTCCATAGCACACACTCCGCTGAGCTTAGCACAACTACTGGATACCAGAAAGGATCTGTTTAACATTTACGGAGAGTTTTTGAAGAATAATAATGTTATTTTTAATAATGCTGCCACTGGACTGGCCACAACAGAAAAGATGGACTTTGAAAAGTTCAAGGCAATCCATGATGTTATCTCATCTTGTGGTGTC GCATAA> P. xylostella- Transferrin (PxTrs); BAF36848.1 (SEQ ID NO: 85)ATGCTGCTTAGGACGATACATTTGTTGTTAATTGTTTGTTGTGCGTGGTGCTATGAAGTGCCTCCGGCTAAATTGGAAGCTATTTATCCAGCTGGCTTGCGAGTGTCAATACCCGACGATGGCTTCTCGCTATTCGCCTTCCACGGCAAGTTGAACGAGGAGATGGAGGGCCTGGAGGCGGGCCACTGGTCCAGAGACATCACCAGACCCAAGAACAACCGCTGGGTCTTCAGCGATAAACAGGCCAGGCTCAAGATAGGGGACAAAGTGTTCTTCTGGACGTATGTTATCAAGAACGGACTCGGGTACCGACAGGATGACGGGGTGTGGACTGTTGAAGGATTCGTCGACGTCGAAGGCAACCCGGTTGACCCCGCGAATGGACAACCCATCTCAGCGCCGACCAGACCTCCAACCCAACCAGGCCGGGTGCCAAATGTCCCCATGCCGTGTGACATCTCAGTCACCACGGCATCAGTGCCAGGGTACATCTGCAAGGGACAGCTGCTCTTTGAAGACAACTTCAATGGGGCTCTGGAGAAAGGAAAGATATGGACGCCGGAGATTATGATGCCTGATGAACCGGATTACCCGTTCAACATCTACCTGAACGACAGGAACCTGCGCGTGAGGGACGGCCGGCTGTCTATCAAGCCCGTCACGCTCGAGTCCAAGTACGGGGAGGAGTTCCTGGCCAAACTAGACTTGTCTGCCAGGTGTACTGGTAACGTGGGTACTACCCAATGCAGCAGAGAGTCCATTGGGGCCCAGATCATACCTCCGATAATCACAGCCAAGGTTACCACCAAGAACAAGTTCAGCTTCAAGTATGGAAGGATTGAAGTGAGCGCCAGAATGCCGCGCGGTGATTGGTTGATTCCAGATATTCTGCTGGAGCCGAAAGAAAACCTTTACGGAGTACGCAATTACGCGTCAGGTCTACTCAGCATAGCCTCAGTCAGAGGAAACACTGCTTACTCGAAGACCCTCAAAGGAGGCCCCATACTGTGTGACAAGGAACCGCAGAGAAGTGCCAAGTTGAGCGAAAAAGTTGGATATGACCATTGGAATAAAGCCTTCCATAACTACACCATGATTTGGGCACCAAGTGGCATCACCATGCTGGTGGACGGCGAGCAGTACGGGGACATCCGTCCCGGCGACGGCTTCAGCCAGGACCCGGCGGTGAGCAGCGTGGTGGCCGCGCCGCAGTGGCTGAAGGGCACCAGCATGGCGCCCTTTGATGTTATGTTCTACATATCCCTTGGTCTCCGCGTGGGCGGAGTGAACGACTTCCCCGACACTCCTGAGAAGCCGTGGAAGAACAAGGCCACTAAAGCCATGCTGAATTTCTGGAACGCCCGGGAACAGTGGCAGAGCAGCTGGTTTGAGGACACCACTGCACTCCTCATAGACTATGTCAGGGTTTATGCGCTGTGA> P. xylostella- Gloverin (PxGlv); ACM69342.1 (SEQ ID NO: 86)ATGTACCGATTTGCAGTTATTTTATCTGTAGTCGCCGCGTGTGCCGTGGCTCAAGTTTCTCTACCTCCTGGATATAATGATAAATACCCAGGCTTCTACAAATACTCCAAGCTAGCCCGGCATCCGCGACAAGTGACGTGGGACAAGAATGTCGGCCGTGGGAAGGTGTTCGGCACCCTCGGCGGCACTGACGATAGTCTCTATGGTAAGGCGGGCTACCGTCAGGACATCTTCAACGACCACCGCGGCCACCTGCAGGGTGAGGCTTCTGGCACCAGGGTACTCAGTCCCTACGGAGACAGCAGTCACCTGGGCGGTAGACTCGACTATAGCAACAAGCACGCCAACGCCAACCTGGATGTCAGCAAGCGGATCGGAGGCGTCACTAGTTGGCAAGCAGAAGGCAAGGCTAGATGGCCGATTGGCAAGAACAGTGAGCTATCAGCCGGCGGAATGATCAGACAAGACCACTTCGGCCACGGGAGACCAGACTACGGAGTCGTCGGTGGGTTTAAATCTAGGTTTTAA> P. xylostella- Chitin synthase 1 (PxCHS1); KX420688.1 (SEQ ID NO: 87)ATGGCGACGTCGGGGGGAGTGCGGGGGCGGCGGGAGGAGGGCAGCGACAACTCGGACGACGAGCTGACCCCGCTCCAGCAGGAGATCTACGGCGGCAGCCAACGCACAGTACAAGAAACAAAAGGATGGGATGTGTTCCGAGAGATCCCGCCGAAGCAGGACAGCGGGTCGATGGAGAGCCAGCGCTGCCTGGAGATCACCGTGCGCATCATGAAGATCCTGGCCTACCTGGTGACCTTCGTCGTGGTGCTGGGTTCAGGGGTGCTGGCCAAGGGGTCTGTGCTCTTCATGACCTCGCAGCTGAAGAAAGATAGAAGACTGGCGTATTGTAATAAGAATTTAGGTAGAGATAAGCAGTTTATAGTGACGTTGCCGGACGAGGAGCGGGTGGCGTGGATGTGGGCGCTGTTCATCGCATTTATGGTCCCCGAGATCGGGACCCTTATCAGATCTGTCCGGATATGCTTCTTCAAGTCCTCCAGAACTCCAAGCAGCGCTCAATTTATTGTGATTTTTGTATCGGAATCTCTCCACACCATCGGATTGGCGCTTTTGATGTTCAAAGTGTTGCCAGAAATCGACGTGGTCAAAGGAGCTATGATAACGAATTGCCTCTGCATCATTCCAGCCATTCTGGGGCTATTGTCTAGAAACTCAAGGGACTCGAAAAGGTTCATGAAAGTTATAGTAGACATGGCTGCGATTGGGGCTCAAGTCACAGGATTCATATTATGGCCACTGCTGGAGAATAAGCCGGTCTTATGGCTGATACCGATCTCGTCAATCTGCATATCACTAGGCTGGTGGGAGAACTATGTCACTCGGCAGAGTCCAATCGGTATAATCAAGAGCCTCGGCCGCCTCAAGGAGGAGCTGAACCACACGCGCTACTACACGTACCGCTTCATCTCCGTGTGGAAGATCCTGCTGTTCCTCATGTGCATCCTCACCAGCATCTGGCTGGACGGCGACGAGCCCGGCATGTTCTTCCAGCTCTTCAGCGAGGGGTTCGGACCGCATAACATTGTTGTCGAAGAGATCCAACTCCAGACGGGAGGCACAATGATCCCGGACTTAGCCAACGCCACACTAACCGGAGACTCAGTGGAGGTGGCAGCGGCCTACAACTCTGCCGTCTACGTCATCCTCATACAAGTGTTTGCCGCTTACTTCTGCTACATATTCGGGAAGTTCGCCAGCAAGATCCTGATCCAAGGGTTCAGTTACGCCTTCCCGATCAACTTGGTCATACCGCTGGTCGTGAACTTCTTGATTGCTGCTTGCGGTATCCGGAATGGTGATACGTGCTGGTTCCATGGGACTATTCCGGATTATCTGTTCTTTGAGAGCCCACCAGTGTACTCACTAAGCGACTTCATATCCCGCCAAATGGCATGGGTTTGGCTGCTATGGCTTCTGTCTCAGACGTGGATCACCATCCACATCTGGACGCCCAAGGCCGAGCGTCTGGCGTCCACGGAGAAACTGTTCGTACTGCCCATGTATAACGGACTGCTCATCGACCAAAGCATGGCGCTAAATCGTAAGAGAGATGACCAGAAGGATGTTAAGACTGAGGATCTGGCCGAAATCGAAAAGGAGAAGGGCGACGAGTACTACGAAACTATTTCTGTGCACACCGACAACACTGGGTCCTCTCCCAGAGCCGTAAAATCTTCCGATCAGATCACAAGAATCTACGCATGCGCGACGATGTGGCACGAGACGAAGGACGAGATGATGGAGTTCCTCAAGTCCATCCTGCGGCCGGACGAGGACCAGTGCGCGCGCCGCGTCGCGCAGAAGTACCTCAGAGTCGTGGACCCCGACTACTACGAGTTTGAAACCCACATATTCTTGGACGACGCTTTCGAAATATCGGACCACAGTGACGACGATTCCCAGGTGAATCGATTCGTGAAACTGTTGGTGGACACGATTGACGAGGCTGCGTCAGAGGTGCACCAGACTAATATTCGTATGAGGCCGCCGAAGAAATTACCTGCCCCGTACGGGGGACGGCTGACCTGGGTGCTGCCTGGGAAGACCAAGATGATCTGCCACTTGAAGGACAAGGCCAAGATTCGACACAGGAAGCGATGGTCTCAGGTGATGTACATGTACTACCTGCTCGGCCACCGTCTCATGGAGCTGCCCATCTCCGTGGACCGCAAGGAGGTGATGGCTGAGAACACGTACCTCCTGACACTGGACGGAGACATCGACTTCCAACCGCACGCTGTCAGGCTGCTGATTGATTTGATGAAGAAGAACAAGAACCTGGGCGCTGCTTGCGGACGCATCCATCCTGTTGGCTCTGGGCCAATGGTGTGGTACCAGATGTTCGAGTACGCGATCGGTCATTGGCTGCAGAAGGCGACGGAACACATGATTGGCTGCGTGCTGTGTAGCCCCGGATGCTTCTCGCTCTTCAGAGGGAAGGCTCTCATGGACGACAACGTCATGAAGAAATACACGCTGCGATCCGACGAGGCTAGGCATTACGTGCAGTACGATCAAGGGGAGGATCGTTGGTTATGCACATTGCTGTTACAACGAGGATACCGAGTAGAGTACTCAGCCGCCTCCGACGCCTACACGCACTGCCCTGAAGGTTTCAGCGAGTTCTATAACCAGCGTCGTCGCTGGGTACCCTCCACTATCGCCAACATCATGGACTTGCTTGCCGACTACAAACATACCATCAAAATCAACGACAATATCTCCACACCGTACATCGCTTACCAGATGATGTTGATTGGCGGTACGATCTTGGGCCCCGGAACTATATTCCTTATGTTGGTGGGAGCCTTCGTGGCTGCGTTTAGAATCGACAACTGGACTTCATTCGAATACAACCTCTACCCGATATTGATCTTCATGTTCGTTTGTTTCACGATGAAATCTGAGATACAGTTACTGGTGGCACAAATACTCTCTACGGCATATGCAATGATAATGATGGCGGTAATCGTGGGTACAGCTTTACAATTGGGCGAGGACGGAATAGGTTCGCCATCGGCTATCTTCTTGATATCACTGTCAAGTTCATTCTTCATAGCCGCTTGTTTGCATCCTCAAGAGTTTTGGTGTATCGTCCCCGGTATCATTTACCTTCTGTCTATTCCTTCTATGTATCTCCTGTTGATTTTGTATTCGACTATAAATCTTAACGTCGTATCTTGGGGTACCCGAGAGGTGCAGGTTAAGAAAACTAAGAAGGAAATCGAGCAAGAAAAGAAAGAAGCGGAAGACGCAAAGAAGAGTGCGAAACAGAAGTCTTTACTCGGGTTCTTGCAAGGAGCAAACCAGAATGAGGATGAAGGGTCAATAGAGTTCTCATTCGCGGGTCTATTCAAGTGCATGTTGTGCACACACCCTAAAGGCAACGAGGAAAAGGTGCAACTGTTGCATATCGCATCTACACTTGACAAGCTCGAGAAGAAACTGGAAACTGTTGAAAAGACCCTCGACCCTCACGGCCTCCACAGAGGTAGGAAGCTGTCGATAGGCCACCGCGGCAGTACCAACGGAGACCACGGGCTGGACGCCCTGGCTGAAGACAATGAGGACCACAACCTCGACTCTGACACCGACACTCTATCCACGGCACCTAGAGAACAAAGAGACGAATTAATAAATCCATACTGGATTGAGGACCCAGAATTAAAGAAGGGAGAGGTAGACTTCTTGAGTCAGTCCGAGATTCACTTCTGGAAGGATCTGATTGATAAGTATCTGTACCCGATCGATGCCAATAAGGAGGAGCAGGCCCGTATCTCGCACGACCTGAAAGAGCTGCGAAACTCATCCGTCTTTTCCTTCTTTATGATCAATGCCCTCTTTGTTCTCATCGTATTCTTGCTGCAACTGAACAAGGACAACCTCCACATAAAGTGGCCCTTCGGAGTCAAAACTAACATTACGTATGATGAGGTGACGCAAGAGGTGCTGATCTCCAAGGATACCTGCAACTAGAGCCTATTGGTCTGGTGTTCGTGTTCTTTTTCGCATTGATTTTAGTCATCCAGTTCACTGCCATGTTGTTCCATCGATTCGGAACTTTGTCGCATATATTATCGTCTACGGAACTGAACTGGTTCTGCAATAAGAAGGCGGAAGACTTATCTCAAGACGCACTGCTAGATAAGAATGCGATAGCAATAGTGAAGGATCTCCAGAAACTAAACGGGCTCGATGACGGGTATGACAATGACTCGGGGTCGGGCCCGCACAATGTGGGAAGGAGAAAGACGATACACAACCTGGAGAAAGCGAGACAGAAGAAGAGGAACATAGGAACGCTCGACGTCGCTTTCAAGAAGCGATTCTTCAACATGAACGCTAATGAAGGACCAGGAACACCAGTTCTGAACCGCAAGATGACGTTGCGAAGAGAGACGTTGAAGGCGTTGGAAACGAGGAGGAATTCTGTGATGGCCGAACGAAGGAAGTCGCAAATGCAAACACTTGGAGCTAACAACGAATATGGAGTCACTGGAATCTTAAACAACAACCCAGCGGTGATGCCGCGCCACCGGCCGTCGACAGCCAACATTTCGGTCAAGGACGTCTTCGCGGAACCCAACGGGGGACAAGTGAACCGAGGGTACGAGACCACGCACGGCGACGAGGGAGACGGCAACTCCATCAGACTGCAGCCGAGAACCAACCAGGTCTCCTTCCAGGGGAGATACCAATAA> P. xylostella- Beta tubulin (PxβTUB); KX420688.1 (SEQ ID NO: 88)GCGGCAGCCAGCAGTACCGCGCGCTGACCGTGCCCGAGCTCACACAGCAGATGTTCGACGCCAAGAACATGATGGCGGCTTGCGACCCGCGCCACGGCCGCTACCTCACCGTGGCCGCCATCTTCCGCGGACGCATGTCCATGAAGGAGGTCGACGAGCAAATGTTGAACATCCAGAACAAGAACAGCAGCTACTTCGTCGAATGGATCCCGAACAACGTCAAAACGGCCGTGTGCGACATACCGCCTCGTGGACTGAAGATGTCTGCCACCTTTATCGGGAACACGACAGCAATCCAAGAGCTCTTCAAGAGGATTTCTGAGCAGTTCACTGCTATGTTCAGGAGGGAAGCGTTCCTCCACTGGTATACTGGTGAAGGCATGGACGAGATGGAGTTCACAGAGGCGGAGAGCAACATGAACGACCTGGTCTCCGAGTACCAGCAGTACCAGGACGCCACGGCTGAAGACGAGGGAGAATTCGACGAGGATATTGAAGACGAGTGA> S. frugiperda-Peptidoglycan recognition protein 1 (SfPGRP1); rep_c7951(SEQ ID NO: 89)GCATTAACATCTCAGGATTTGATTCGAAAGTAAGGTCTGAATTTAGTCCTTACATTTACTTAAAATTAGGTCCTTTTTGGTTTGTACTGAAATGAAAGTTTTTCTGTTCTTGGTCTTAATTGTGAAGATAATGGCTGAGGCAAAAGGAGATTGTGATGTGATCCCGATTACGCAGTGGGGAGATTCACCTCTTAAAAGGGAGGATYCTCTTCCAAATCCAGTGAATATTGTTGTCGTCCAAYACRCTGTGGTACCGGAGTGTAACAATGATGAAGAGTGTGAGAAAGCAGCCAMTGGAATCAGGAGCTACCACATTAACAAACGTGGATTCACTGATATAGGACAATCGTTCCTGATTGGTGGAAACGGGAGAGTTTATGAAGGAGCCGGCTGGCATCACGTTGGGGCCCATACTTTGGGATACAATGCAAGATCTGTGGGGATCTCCTTCATTGGCGATTTTAGAACAAAATTACCAACACCCGAAGCACTGAAAGCCTTCAACAGTCTCCTGGAATGTGGAGTCACGAACAATTATCTGTCAAAGGACTATCACCTGGTGGCCCATAGTCAGCTCTCTATGACTGACAGTCCYGGAGACATGYTGAGGAAGCAGGTGGAATCGTGGCCTCMTTGGCTGGATAATGCCAAAGACATACTTAAGTAGAARAAGACTAAACGCCGTACTTTGAGCCATTTAATGGTTACTTAACCCAGTCCTTAGCAATTTGATACAAGGCCAATGTCTCTAAGGGCGGCAGTAAAGGTCAAAACACATTTAATGAGTGTGTTTAAGATTTTGCTAGTGAAAATTGTTTTGAAGTACGTATTTGATGTAAGTGATGATATCAGTACCCTTAGTATGAGTTTGCTTTACGTTCCACGAGATGGAAACGAGAGCGCGTTCGGCGCTCTGATTGGTTCGTTCATTCATGCCGGCCAATCATAGCGCCGAATGGGCTCTCATTTCGTTTTCGTTCAACGTAAAGTAAATTCGTACTAAGGGTACTGACTTTAAAATAAGTTACCAAAAAGAGTATTACCTATTTACATTATTTTATTTATTTTAGGTGTATTGTAATTCAAGTATTAAATTAATTAGTGTAGATTAATKSCATGCATTTTATATTTGATTTCATT GAATAAS. frugiperda-Attacin (SfAtta); rep_c9395 (SEQ ID NO: 90)ATCATTCCAGATCCTCTCTCATACATCCAACACTTGAAGCAAACCAATCCACATACATTATAGCAACATGTTCGCTCTCAAGTTGGTACTAGCTGCAGTGCTGGTGGTCGCAAGCGCCAGACATCTACCACAGGACCACTCAACGTACGACCAAGTACAACTCCTCGGGTTCGACGAAGATGGACGACCAGTGTTTGAGCACGAAGACTTACTCCCAGAACTAGAGGAGTCCTACCAGCCAGAGCACCTGGCGAGGACTCGCAGACAGGCGCAGGGCAGCGTCACCCTCAACTCCGACGGCGGCATAGGCCTGGGCGCTAAGATCCCGCTCGCACACAACGACAAGAATGTGGTGAGCGCCATCGGCTCCATGGACTTCAACAACAAGTTGCAGCCTGCTTCCAAGGGCTTCGGTCTGGCTCTGGACAACGTCAACGGGCACGGACTGACGGTGATGAAGGAAAGTATCCCCGGGTTCGGGGACAGGCTGTCGGGCGCTGGCAAGCTGAACGTGTTCCACAACGACAACCACAACGTGGCCGTGACCGGCTCTCTCGCCAGGAACATGCCCAGCATCCCGAACGTGCCCAACTTCAACACGTACGGCGGGGGCGTCGACTACATGTACAAGAACAAGGTGGGAGCGTCTCTGGGCATGGCCAGTACTCCGTTCTTGGACCGCAAGGACTACTCCGCGATGGGCAACCTGAACCTGTTCCGCAGCCCGACCACTACCGTGGACTTCAGCGGCGGCTTTAAGAAGTTCGAATCTCCCTTCATGAGCAGCGGCTGGAAGCCTAACTTCGGCCTTACTTTCGGCAGATCTTTCTAGATATATTTTGTAATCTAAATTTAACTTTAACTTTGTTGTATAATATTTTGTCGAATTAAGATCAGTATTGTTCATACTAATATTATATTATCAGTGTTTCTTATAAATTAAS. frugiperda-CtypeLectin15 (SfCTL15); Joint2_rep_c488 (SEQ ID NO: 91)AGTTTTCGTGCTTAGCACACAGAGCGCCGAACGCGCTCTCGTTTCAATTACTTTAAACGTAAAGCAAACTCGCACTAAGGTTACTGTGACCTTTGTAAACATGTACAATGCAATACTATGTTGTTATTTTCGATACAATCATATAAAAGACAACGTTTAAAAAAAAAGCATAGCACAGACCACAGTACAAGTATCGAGACCAAACTTTTGTTAAAACTTATTTCGTTCTGTGCAGCAACTCTAATGCGAATAATGTGCAAATTATAATAAATATGTTTTGTATATAAACATGTGTTATCTCAAGTTCAAGACATTCCGGCTCCTACAGTCAACAGATACAGTGCACAAAAATGTTAAAAATATATTTAATTTGTTTGTCTTACTTGTTCATGTTAAACTTAGACAGGGCACAATGCACCCCCCAGTATTGGTTCAACATGGATGCGAATGGTTGGCTGAAAGTGCACACGATACCCGCCACGTGGGAGGAAGCATTCCTTCGGTGTCACTATGAAGGTGCGGTACTTGCGTCCCCCTTGACGCAACAACTGAGTAAGGCTCTTMAAAACAAGTTTGCAGKCWTCGGTAACCCATCAATCCACTTGGGGACACATGATTTGTACTCCASTGGTTACTACTTTTCTGTAGAAGGAGTACCAATGGACAGCTTGGTGCTGAAATGGAGTAATATCAGAGGTACGGGCGAYTGCCTGGCGATGTCACGCGACGGGGAAGCATTTTTCACGAAATGCGAGCAACCTCGACCCTACATCTGCTACAAGAAGCTGGACAATCTGACGATGAACATCTGTGGAACATTYGATGACGCKTATCAATTCTACGATAAGACTGGCAGCTGCTATAAGAGACACAACGYCTATCAGACATGGCCTGACGCGTTCAAGATATGCGCTGCCGAGGGAGGSTACCTGGTGATCCTGAACGATGACACAGAGGCCGCCATCATCAAGGATATGTTCCCCGTACGGCCAGGAAAGCCCAACGAATGGGAGAACTTCCACGTGGGKCTGCGGGCATGGGGACCGGAACGTACTTGGATCACTATTCATGGAGAAAAAATTGATGATGTATTCCATAATTGGAACCCGGGACAGCCGGACAACTACAAGGGAGTCCAGGACACTGGCGCTTTCCTTAGARMAGGCACTTTAGACGATCATGCCGCTGGTGACAAATGTATGTTTGTCTGTGAGAAGGATCCTAAAGTAAAACGCTTCGAAGAGYTACCCGAAGGATTGGCMGAAGTTTTAGGACAATAGGCCGCTAGTCAAATATTGTTCATATACCTWAGTTTTAATTATGTAAAAATAATARTCGATTGCAGAATTTAATAAAATTTAAACTAAAAAAAAAAAAAAAAAAAAGGTMAAAACATGTC S. frugiperda-Galectin4 (SfGlc4); rep_c2653(SEQ ID NO: 92)GCCTTGTAGTTCGACAGTCAAATCCAACGGGTGTCAGAAACAATTTCCGTTTTTCCGGCTATTGATCGTCATATAAATAACTCCGAAGGAAAAATGACTACAATCGACAATCCGACAGTACCGTTCACGAGACCCATTCCTGGGCATCYGCTCCCCGGCCGCAAAATGGTCGTTAAGGGTGCAATCTCTCCCAGATCAGATAGGTTCTCAATAAACTTGAAATGTGGTAGCGAGGACATCGCTTTCCACTTCAACCCTCGCTTCAGTGAGCAGAAAATAGTTCGCAACTCTTATATTTCTGGCAAGTGGGGTCATGAGGAGATCAGTGGAGGCATGCCGTTGGTAAGGGGAGAGCATTTTGAAGCGCAATTTGAATGCAATGAAGATAATTTTTCGGTGGAGTTGAACGGGAAACATTTCTGCAATTACTCTTATCGCATCCCAATCCATAAGATCACCCACGTCAACGTGGACGGTGACGTCACGATAAGTCAGATCACCTTCGTGGACGCCTGAGCCATCGCCCGATCTTACAATGGTACTTTTTTTTTACTTATGAACTAATGCTGGAAACCAAACTTCTTTATTCAAATATTTTTCTTTTGTCTTTATGACTATCAGTGTTTTTATTGCAATAAAAAGS. frugiperda-Lysozyme (SfLys); rep_c18992 (SEQ ID NO: 93)ATCAGTGTGGTGTCTTCAACCCAAGTGCATTGTATTGCTTGGTAAATAATAACTGASAACAAAAATCTTGGTTATTGCTTGAACAGGTTTCGCCTATCAATTAGCCGAATATTGTTACCCTTGACACGCAATAATTGGTTACTCGATTAAAGCTTGAAGAGGTAAAGTGCCGAAATGACGCGCGCCATTCTGTTTGTTGTTGTTTTATGCTTTATTGCAAAATGCTATGGAAAAACATTCACKGAATGTGAATTAGTTCAGGAGCTAAGAAGGCAAGGATTTCCTGAACATGAGCTTAAAGATTGGGTRTGYCTGATCGAAGCGGAGAGTTCCAAACGAACCAACGCCATTGGTAACGGCAATTCAGATGGCTCTCTCGACTATGGCTTATTCCAAATCAAATAACCGCTACTGGTGCAGCGAYGGTGACCATCCAGGCAAGGGATGCAACGATACCGCTAGTAAAGATCTGTTGCTGGATGACATCACAATAGCGTCTCAGTGCGCTAAGACCATTTTCGGTGTCCACGGATTTAACGCCTGGGTCGCATGGGTGAACAAATGCAAAGGAAGGACCTTACCYAACCTTCACTGTTAGTTATTTATTGAGAAAATGTAACTAAGGTATATGGTTACTTTGTACCTAAATATAGGTATTAGGCATATATTGTCCACTCTCATCAAATTTTTACTTTTATATCAGTS. frugiperda-Hemolymph proteinase 10 (SfHP10); c12881 (SEQ ID NO: 94)ATTCGCTGTACGCACCTCGGATTGTGCGGTCAACTWTACAAGGCTCGTACCTTGCAGTTGAACCGACAATCTATTCCTAAAGCCTTTTTAAGGTCAGGAAAAATAGTTCCTACATCTAAATGCAGTAGAATTTGCGAAACGAATTTAAATAAAAATGGCGTCGATTGTGTTTGTGATTTTGTGTGTTACCGTCGCTGCGGTGAAAAGCGCGATTTTAAACCCGTGGAGTAAAGTTGAGGCCAACAAATGTGGTGTAGAAGCCAGTACTAACTTGGTCCATCACAATCCATGGTTGGTCTACATCGAGTATTGGCGTGGAAACTCAGATACTGAGATCCGATGCGCCGGTACTTTAATCGACAGCAAACATGTCGTCACAGCTGCCCACTGCGTTAGGACTCTGAAGTTTAGTCATTTGATCGCCCGTCTTGGCGAATACGACGTAAATTCTAAGGAGGACTGCGTTCAGGGCGTGTGTGCCGATCCCATCGTCAGAATCAAGGTGGCTGAGATCATCGTGCATCCTAACTACAGCAACCGGGAACATGACATTGCAATCTTAAGGCTGGAGGAGGAAGCTCCTTATACCGATTTCACTCCGGCCCATCTGTCTGCCTTCTGGTGATCTCGCGGAAGACACCCAGTTCTTAGCAGCCGGCTGGGGTGARATCCCCACGAAAGGCTTCTTCAGCCACGTGAAGAAAATCGTCCCCTACGTACTGGAATCGAGAGAGATGCCAAAAGGTGTACCAGTACAATTATATCCCGGAGAACGTGATC TGTGCCGGTS. frugiperda- Trypsin like serine protease (SfTSP); rep_c48453(SEQ ID NO: 95)CAAGTAGCAACAAAATGCGTGTCCTCGCTTGCTTGGCCCTTCTCTTAGCTGTGGTAGCAGCCGTCCCCTCCAATCCCCAGAGGATTGTGGGTGGTTCGGTCACCACCATTGACCGGTACCCCACCATTGCATCCCTGCTGTACTCGTGGAACTTGAGTTCCTACTGGCAGGCGTGCGGTGGTTCCATCTTGAACAACCGTGCCATCCTTACTGCTGCCCACTGCACAGTTGGTGACGCCGCCAACAGATGGAGAATCCGTCTTGGCTCCACCTGGGCCAACAGCGGTGGTGTCGTTCACAACGTCAACACTAACATCGTCCACCCCTCATACAACTCTGCAACTTTGAACAACGACATCGCTATCCTCCGCTCCGCCACCACCTTCTCCTTCAACAACAATGTTCAGGCTGCCTCCATTGCTGGTGCCAACTACTTGCCCGGTGACAACACCGCCGCCTGGGCCGCTGGATGGGGAACTACCTCCGCTGGTGGCTCTAGCTCTGAGCAGCTCCATCACGTTGAGCTGCGCATCATCAACCAGGCTACTTGCAAAAACAATTACGCTACCCGCGGTATCACCATCACCTACAACATGTTGTGCTCTGGCTGGCCCACCGGTGGTCGCGACCAGTGCCAGGGTGACTCTGGTGGTCCTCTCTACCACAACGGCATCGTTGTTGGTGTCTGCTCTTTCGGTATTGGCTGTGCTCAGGCTGCCTTCCCCGGTGTCAACGCTCGTGTATCCCGCTACACTGCCTGGATCTCTTCCAACGCATAAGATGTTACTTGGTGCTAATAATATTTTTTTGTAATAAAATGTTACTTTTATCCTCCS. frugiperda- C Type Lectin 6 (SfCTL6); Joint2_ rep_c448(SEQ ID NO: 96)AACAGTTTTCTATTGGCAGTCAAAGACTTCAGTCGAAAAATAATCCTCATCAGAGTCGTGAAGCAAGGTGCCCAAAATATAATGTAACCTAGATACCTATTAATAAATTATTTGTCAACCAAAACGTTACGTTCAAAGTCCTTAAAATCAAAATATCTTATGATTAGTTTTGATTTAAAAATAGAGGTTCGAAATCGCCAACCCAAATAGGTTTAGTTTACGATTCAGGAAAAATCCTAACGTAGGGAAACATTATTTTACAAGACTTTTGGCTTAAAAACTTTGAGAACCAATGTCAAATTTGATAATAACTAATGAGGTATAAAAGCTTGATCCTATTAGGACTTATTTTCATAACACCATCGAGTTTGTATTTAATRRAGACGTGKGTTAACTAACAACATGAAGACCGGTGTAAAATATTCTGTTMTTTGGATATTCTCTCTATTYTGCTATATAGAGGCAACATTTCGTTGTGACTACACGTACAGCAAGGAAGCGAAGGGCTGGTTCAAACATGTGGTGATACCAGCTACTTGGGCTGACGCACGWCTGCACTGCACGTTGGAAGGTGCAACGCTGGCTTCTCCACTCAACCAGGCTATMAGTAATGAGATGCAGTCCMTCCTGGCRAACCTCTCGGCGCTGCAATCAGAAGTCTTCACTGGAATTCACRCGACTKTTTCACGRMRCAACTTATATCATACYATYGAAGGTATACCTCTTAGTAAAATTCCATTAGATTGGGCAACAAATGAGCCAAATGGTGGGAGAGATGAAAACTGTATCACGTTTAACTCCGATGGCCAAGCGGCAGACAGATCCTGTAARGAGACTCGACCTTACATCTGCTACCGACACACWACTAAAGTGACTGTGKCCAATGAATGTGGGACTGTAGATCCTGAATACAATTTGGATAAAAGAACGGGCKCYTGCTATAAGTTCCACACRGTACCTCGCACGTTCGAGCGTGCCAACTTCGCGTGTTCTGCTGAAGGTGGMCACCTTGCCATAATCAACRGTGATRTCGAAGCTGCAGTACTGAAGGAACTCTTCGAAAGGAATCCACCTGCAAAGATGTTCGGTACTTTCTGGAAAGACGTTGCTTTCATTGGTTTCCATGACTGGGGTGAATATGGTGACTGGAGGACAATTCACGGTCAAACGCTAGTAGAGGCAGGATACAGCAAGTTTTCATCTGGAGAGCCAAATAATTCCTCGACGGGAGAATTCTGTGGTTCCATCTATCGGAATGGTATGCTCAACGACCTTTGGTGTGGGCATCCTAAACCATTCATCTGTGAGAAGGACCCGAAATATCCAGCCGTATGTTGTGTTACAGAATCAGAACCAGAATTGGACCCTACTCACTTCTTAGAGTAATTCAAATTGGATCTTTTTTTATAATTCTACATCATTAGAAATATGATGTTTAGTTGAATCTGCTTTTGAAGATTGATTS. frugiperda- Serine protease homolog 13 (SfSph13); rep_c1904(SEQ ID NO: 97)GAAKAGTGTCAATTTATTTAATTGTCAAAATCGCGTTGAATGTAAGATTGCTATAGAAATTATTTGTAAAAACTTTCTCAAAAATTTAATTCTAAAATGTAAAGGAACCTAAAATCAGATACCGATAACACATTTTGTAAATGGTTTAATATTGAAACGAAACTTCTAAACATTTTTGGTGAAATACACATAATAATATAACTTCTTCGAAAACTAGGTAGATAGACTTCAACTCAGTTTTTATTAGCGAGCTAGAAGATACAGAGATTATCTAAGATGGTGTGTGATGGTCCGGATCCTCCAGAGAACAATCCTCACGAAATTGTTCCACCTCCTAGGAATGAATTGATAATGAATGGTAATCAAGGGGATGACAGTGATGAAGAACATGAATATTTTGGCTATGAACCTTTGGCGCAAGGTCCAGAACTAGCAGTCTCAGATCACGATAGTGATGATGACCCAGAGAGTGCTGAAACACCTCCAGCTGATGTTCCAAATATAGAGCCAATGGAAAATGTACTAAGCCGGGAAGTTTGGAGTGCCCCGAGRCATACAGATGCTATACAAATGGATAATGAACGGGCTCAACAGGTGATGAGAGCTATGGCAAATTTTGCTCTACCCCAGGCCTCAWTTCCAGAATGGGCTCAGAGCATCTCTGAAGAACAATGGAAGCAAACTCTGAATGAACAAATAGAAAGATTGAAAAATAAAAGATAAACTAATTATATGTAGTATAATAATAATTAAGATTTAAGTGATAAAAATAACAATTATAACTTATATGTTTAAAAAATGTATATAAACTGTAAACTTTAAGCTTATTCACATTACTTTAAATATAGAAAAAATATGTGTACCTATTTATTGTTTGGCTACATAATCCAATATAGATTAAATTGCAAATGTTTAATGTAATTATTTAGTTGGATCAAAAATAACAGCACTAAAGGTCACTAGTTTAGTTCTTTCATTTCGAATGAAAAAGTAAAATGGAGAATCTGCATAGAAATTTTCAGCTGACCGTGTTAATGTTCCACTTGTTGCGGCTACTGCTTCTGTTCCAAACTCATTAATTGTTAACTTTACATCATGAATTATGGAATCCACATGTATTCCAGGGTTGGGTAGACTGTCCAAAATGTTTGTCCCATTTCCTTCGTTTTCTGAGTATCTGTCTGATTCTCCTTCAGATTTATTCACAAACTGATTGGTGTTATTTACCATAAGTGCGAAGTTTGCTTGGCCTGGTATAAACATACTTTGTATCCCAATAGATTGCAAGGTGCTCTCTAGCTTTGTGGTGCTTTTAATTTTCATCTTAGGGAAACGAATAACACAATTTTTCACATGCATTTCATTGATGGTATCATCAATAACTTTGTTGTTAATATTTTCCATGAGTTCCAATAATGTTAATCGYTTATGAGATAATGGTTTTAAAGCGTACATAAAAGTTTCACTGTCGTTATATGGCAATGCTATCATGTGGAAATCATGTTCCTCAGAAACCTTGTAACGAAACTCCCCAAAATTCAACATCATGTCAGCCATAACTTCATCTGTGGCCCTTTTGAATTCCATCTTTCTTGTGAATTGCGGTGCAAAAGGCTGTTTCCATGTGCCACTAAAGTACAATGCAGTTAAAAGTACTACTTTAGTATGGGGAGGCAGAGAATCTTTCAAAAAATCGTCTATATTTCCTTCTGTGTGCTTGCTCACCCACTCATTGATGCTGTTTTTAGATCCTTCGGTTTCTTGAAAATCAGTACGTAACACATCGCCTCCGTAAACACCATGCAGATAACTTCTGTACAACTCTCGCAATTCTACTTGGTTGTCTACAAATATCGCGTCAGCGTACAAAGTTTTTGAAAACTCGTTCATATTTAAACTCTGTAGTAGTTCGCCGAACTGTTCGTGGTTCTTGCGGTTTCGTAGAATATCTTGGGAAAATCCGAGTGTCTCTGCGATTTCATCATGTGTCATCCCAACACTTCCGAGTAATGTCATGGCAAGCAGACCAGCGACACCTATTGGTGATATCACGATGTTTTCGTTTTTCTTTTCGTTCATCATTTTGACTAGTAAATTATATCCAAAATTATTTATAGCTTTTGGTATCGAGTTATCTTTCGTATTTGTTGTTTCTAAGTTATCATTTTCAGCAGTTGTGGTTGGTATTATCGTGCTTGAAATCTGACTGTCCTCTTCATTTTGTCCAGAATATTGAAAATATAATGTTGTTATAATTAAAAATAACAATGACTTGCTATCCATGTTGTGGTGCTTAAATAAGGTATTAAACGTAAACTTCACACTAACAGGAACAGGTTCCTATTATTTCCCACTTAAATCGTGAACTTACGGCATAGACACTATACACGTCTACTCGTTTGACCAACTCTGAGAGCAACTCAAAAACAACCTTGTGTGTGTATGAATAACGAACTAAAA TS. frugiperda- Cecropin (SfCec); rep_c42380 (SEQ ID NO: 98)TTCGTGTCGTATCACTAGAGTTCGAAATACAAAATAATAATACATTTATTATTTTGCCATAATTAATAATAAAGTTATTTTATTTCATAATAATAATGAATTTCACAAAGATATTTTGTTTGTTTTTGTCTTGCTTTGTTTTGATGGCGACCGTGTCAGGAGCTCCTGAACCGAGGTGGAAATTCTTCAAGAAAGTGGAGAAGTTGGGCCAAAACATCCGCKATGGTATCATAAAGGCAGGACCCGCAGTGGCCGTGGTGGGATCAGCRGCAGCCATWGGAAAGTGAKCCCTACGACCTGAGACATGAAGACTAATATCCAYTAAAATAASAATATTGAGGCKTATAATATTAATTTATTRTRTTTGTAAATTAAATTATTTGTAAGATAA S. frugiperda- Relish (SfRel); c13122(SEQ ID NO: 99)ATAATATGAAGAGTGCTGCGACTAACGATTTAAGAATCTGTCGTATAAGTCGGTCGTCCGGAATTGCTTCTGGCGGCGAAGATGTCTTCATTCTGGTGGAAAAAGTTAATAAGAAAAACATAATGATTCGATTCTTCGAGTTGGATGAAAACGGAGAAAAGAGGTTGGACCAATGTTGGGCGATTTGTGCAAAGCGATGTTCATCACCAATACGCAATCGTCTTTCGTACTCCTCCATACAAAAACCCTGAGACGCCAGTCGATGTGGAAGTGTATATCGAACTGGTTCGTCCATCGGACGGCCGTACCAGTGAACCGAAGCAATTCAAGTACAGAGCCAACCAAGCGTACAAACAGATCAAGAAGAGGAAGACTGGATCTTCCTACTGTTCTATTGGCAGCTCATCTAGTGGATCGTTGAAAAGTGGTTGCGACATTCCCATATCTGTTGTCAATCATCAACCAGAAGAAGTTCCCATGGATCGCGAGCCACCGGTGCCGTCGTCGATGTATGTTTTACCCCAGGTGCATGATTCGACGACACCAAACCAATGCGATCTGGCCAGTGCTCTGTACTCTGCTCCTGGTTCGGAGACCAGCCAGTCTCCTATATCGAGTCCGATGTGGAGTGAGCCTCACAGCGTGATGCTGCCGATGTCGCCCATTGCCAACCTTCAGCTCAACTCCGCAGACTTCGAACAGATCACAGTACCCACTS. frugiperda- Toll (SfToll); joint2_ c3284 (SEQ ID NO: 100)AAAACCGAATAACCGGTATACCGGATAACTTCTTCAAGGATTTGAAGAATATAGAGCATTTAGATTTGAGCTTCAATAAGATAACTAAATTGACCAGTGGAGTGTTGTCACCAATGAAACAATTGAAGTATTTGAACCTGAATCGAAAYCATTTGGAAGTTTTRCCTGAATTCCTCTTCGCTGGCCTCAGRAAACTAGARAAWGTRWCAATMAACGAAAATCTACTCACTTCCATAGATTCTTTAGCATTCCAAGGRGCWACGGCWTTGCATACAATATCTTTATACGGCAACAGATTAACATTGAAGTCCAATGAACACATCCAAGACTATATGGACTTAGATCTCTACTCACCCTTYAACACTTTGAGCGAATTAAAAAATTTGAACCTTAGTAAGAAYAAYATAAGCTCTATATTCGATGATTGGMGGATWGTGYTRCTCAATYTGGAGTTGCTGGATCTATCTTATAAYCATATTGGAGAGTTATTGCCGGACACCTGYCAATTCCTAAGCAACAARRTYACAGTGGACCTGCAGCACAATGACATAAGTACTGTTATTTTATATCCTACGTCTCATATGGACTTCACAGAACCCAGCATGCCATCAAACAATGTGTTTCTCTTAGACAACAACCCATTTAACTGTGATTGCCACATATATAACCTAGCTATGCGTCTACAAGGCAAAAAGCTGCCCTACGAACCTACTTTCAACGTAGGCAAGGCCGAATGCAAATCCCCACGCCACTTAAGTGGGGATTTGTTGTCACATGTATCCCCACTCGACTTATACTGCGAAGAAATGTCGCCTGATGTTCGTTTTAATTGTAGTTCGGTTAAAATGAGGCCTGCGTACAATGATTTGGCGTACGACTGTGATGATGTACCACCTTACTTCTCTGATGACATTAAAAACTACTCTTTGAAATTACGACACCCACCAAAAGACTTACGCAACCTAACACTAAGTCTACTAAACCTAACCGGCATAGGATTACAAGAAATCCCMTTTACRCCGTCAGAATCGGTAAAAGTAATCGATTTATCGAACAACAATCTCACAGAGATACCKATMMGATTCTTAGAATCGAATACGACRTTGTWTTTATCGAATAATCCATTTGTTTGCGATTGTTCTTCRAAAGATGATATTTTAGCTTTAMGRGCRAGTTAYAATGTGAATGATTTGGATATAGTYAGCTGTTCAAACGGCGTTTTGGTAACAGATTTAGAAATATCATCGCTATGCTATACAAGAACTTTAATAGCAGAAATAGGTGGTATAYTAATATTCTTAYTAATAATCTTAGTRTTTATAATAACATTYATATTTCCTAGACAAATGGTACTRTATTGTGGTCATAGGCTMTTTCCKTGCTGGTATATCGACGATCCAGAAACTACGGCAAAAGAATATGACATATTCATATCTTATGCTCATAAAGACCAAAAATATAGTGAATAAGCTACTCCCGTAAACTAGAAAATGATTTCAAGTTAAAAGTCTGCGTTCATTACCGAGATTGGGAAGTCGGTGATTTCATTCCGGATCAGATCAATCGATCAGTATCGAATTCGAGAAAAACGATTATTCTTTTATCGAATAGCTTTCTCGATTCGACTTTCGCGAATTTGGAATTTCGTACCGCGCATAATTTAGCTTTGAAAGAGGGAAGAGAGAGGGTCATTTTAATACTTTTAGAGGACGTTAGTAAACATGAGAAGTTATCTGAAGAATTAAAGTATCATATGAATATGAATACGTATCTTACATGGGATGATATTAGATTTGATGAAAAGTTAAAACGAAGGACGATACCACAGAAATATAATAGAAAGAAATTCGTAGCGCCGGCCATTTTAAAACCTATATTCAGACAGGCTACTGAGAATAATTTGAAAAAGGCACTCGATGTACACTTTGAATAGTGCAGGCCAATTGGTGAATTTAGCTCAAAATAAGAAGAATATTGATATGGTATAGCTTTTCCCAAATAAGGCTCTTTTAAACATGTTAAGCCCTCTCTCACCCGACCTCTCTGACTACCGCACTCAGAGACATTTTAATGTATWTTTTTTTTTTAATTGGGTACAAGCCCGCCACAACATCCCAGACCGCTS. frugiperda- Beta 1, 3 glucanase recognition protein (SfβGRP2); EF641300(SEQ ID NO: 101)ATGTGGTCGGTGTTAGCCGGAGTATTGGCGATCGCGTCGCTAGGCGCGGCTTGCACCCCCAGTTTGACCACCGTCAGTGGTACCCACGCACCGGTCACCGTCTGCTCTGGTGCTCTGATCTTCGCTGATGGCTTCGACACTTTTGACCTCGAGAAATGGCAGCACGAGAATACTTTAGCTGGTGGCGGTAACTGGGAGTTCCAATACTACGGCAACAATCGCACCAACTCTTTCGTGCGCAGTGGAAGTTTGTTCATCCGTCCCTCTCTAACATCAGACGAGTTCGGAGAAGCTTTCCTCTCATCTGGACACTGGAACGTCGAGGGTGGTGCTCCTGCTGATAGATGCACAAATCCACAATGGTGGGGTTGCGAGAGAACAGGCACGCCGACCAACATTTTGAACCCAATCAAGAGTGCTCGTGTCCGTACCGTCAATTCCTTCAGCTTCCGTTACGGACGCCTCGAAGTCCGCGCTAAAATGCCCGCCGGAGATTGGATTTGGCCAGCTATCTGGTTGATGCCTGCGTACAACACTTACGGTACTTGGCCCGCATCAGGAGAGATTGACTTAGTTGAGTCCCGAGGCAACCGTAACATGTTCCACAATGGTGTCCATATCGGTACACAGGAAGCAGGCTCGACCTTGCACTACGGACCTTACCCAGCGATGAACGGTTGGGAGCGCGCCCATTGGGTCAGAAGGAACCCTGCTGGCTACAACAGCAACTTCCACCGTTACCAACTTGAATGGACACCAACTTACTTGCGATTCAGTATCGACGACATGGAGCTTGGACGTGTAACCCCTGGCAATGGCGGCTTCTGGGAATACGGTGGTTTCAACAGCAACCCTAACATTGAGAACCCATGGAGATTCGGAAGCAGAATGGCGCCTTTCGATGAGAAGTTCTACCTTATCATGAATGTGGCTGTCGGTGGTACCAACGGATTCTTCCCTGATGGCGTCAGCAACCCATCACCCAAACCCTGGTGGAACGGATCACCAACCGCCCCAAGAGACTTCTGGAACGCGAGATCAGCTTGGTTGAACACCTGGAACCTGAATGTCAACGATGGACAGGACGCATCCATGCAAGTCGACTACGTCCGCATCTGGGCTTTGTAAS. frugiperda- c20042 (Sfc20042); Un-annotated (SEQ ID NO: 102)ATCAGTCGTCCCTCGTACATCCAACACTTCAAACCAAATATCTCCATATACATAGTAACAACATGTTCGCCCTAAAGTTGGTACTAGCTGCAGTGCTGGTGGTCGCAAGCGCCAGACATCTACCACAGGACCACTCAACGTACCGAACATGTACAGCTGCTCGGGTTCGACGAAGATGGACGGCCAGTGTTTGAGCACGAAGACCTGCTCGCAGAACCAGAGGAGTTCTATCAGCCAGAGCACCTGGCGAGGACTCGCAGACAGGCACAGGGCAGCGTCACCCTCAACTCCGACGGCGGCATGGGCCTGGGCGCTAAGATCCCGCTCGCACACAACGACAAGAATGTGGTGAGCGCTATCGGCTCCATGGACTTCAACAACAAGCTGCAGCCTGCTTCCAAGGGCTTCGGTCTGGCTCTGGACAACGTCAACGGGCACGGACTGACGGTGATGAAGGAAAGTATCCCCGGGTTCGGGGACAGGCTGTCGGGCGCTGGCAAGCTGAACGTGTTCCACAACGACAACCACAACGTGGCCCTGACCGGCTCTCTTGCCAGGAACATGCCCAGCATCCCGAACGTGCCCAACTTCAACACGTACGGCGGGGGCGTCGACTACATGTACAAGAACAAGGTGGGAGCGACTCTGGGCATGGCCAGTACTCCGTTCTTGGACCGCAAGGACTACTCCGCGATGGGCAACCTGAACCTGTTCCGCAGCCCGACCACTACCGTGGACTTCAGCGGCGGCTTCAAGAAGTTCGAATCTCCCTTCATGAGCAGCGGCTGGAAGCCTAACTTCTCCTTTAATCTTGGCAGGTCATTCTAGAAATATTTTTAAACTCTTATTTAAAAATTAAATGTAAAAAATCCWGTTTGTTCATGATAATAAGAATAAATRACAGTATTGTTCGTACTATTTACTATGTAATCTATAAATTGTATTAATAAATGAAAATTA AS. frugiperda- rc16438 (Sfrc16438); Un-annotated (SEQ ID NO: 103)ATCAGTCGTCCCTCGTACATCCAACACTTCAAACCAAATATCTCCATATACATAGTAACAACATGTTCGCCCTAAAGTTGGTACTAGCTGCAGTGCTGGTGGTCGCAAGCGCCAGACATCTACCACAGGACCACTCAACGTACCGAACATGTACAGCTGCTCGGGTTCGACGAAGATGGACGGCCAGTGTTTGAGCACGAAGACCTGCTCGCAGAACCAGAGGAGTTCTATCAGCCAGAGCACCTGGCGAGGACTCGCAGACAGGCACAGGGCAGCGTCACCCTCAACTCCGACGGCGGCATGGGCCTGGGCGCTAAGATCCCGCTCGCACACAACGACAAGAATGTGGTGAGCGCTATCGGCTCCATGGACTTCAACAACAAGCTGCAGCCTGCTTCCAAGGGCTTCGGTCTGGCTCTGGACAACGTCAACGGGCACGGACTGACGGTGATGAAGGAAAGTATCCCCGGGTTCGGGGACAGGCTGTCGGGCGCTGGCAAGCTGAACGTGTTCCACAACGACAACCACAACGTGGCCCTGACCGGCTCTCTTGCCAGGAACATGCCCAGCATCCCGAACGTGCCCAACTTCAACACGTACGGCGGGGGCGTCGACTACATGTACAAGAACAAGGTGGGAGCGACTCTGGGCATGGCCAGTACTCCGTTCTTGGACCGCAAGGACTACTCCGCGATGGGCAACCTGAACCTGTTCCGCAGCCCGACCACTACCGTGGACTTCAGCGGCGGCTTCAAGAAGTTCGAATCTCCCTTCATGAGCAGCGGCTGGAAGCCTAACTTCTCCTTTAATCTTGGCAGGTCATTCTAGAAATATTTTTAAACTCTTATTTAAAAATTAAATGTAAAAAATCCWGTTTGTTCATGATAATAAGAATAAATRACAGTATTGTTCGTACTATTTACTATGTAATCTATAAATTGTATTAATAAATGAAAATTAACTATMTAAMWAAAAAAAAAAAAAAAAAAAAAAAAAACATGTCS. frugiperda- j2rc2367 (Sfrc2367); Un-annotated (SEQ ID NO: 104)ATCAGTCGTCCCTCGTACATCCAACACTTCAAACCAAATATCTCCATATACATAGTAACAACATGTTCGCCCTAAAGTTGGTACTAGCTGCAGTGCTGGTGGTCGCAAGCGCCAGACATCTACCACAGGACCACTCAACGTACCGAACATGTACAGCTGCTCGGGTTCGACGAAGATGGACGGCCAGTGTTTGAGCACGAAGACCTGCTCGCAGAACCAGAGGAGTTCTATCAGCCAGAGCACCTGGCGAGGACTCGCAGACAGGCACAGGGCAGCGTCACCCTCAACTCCGACGGCGGCATGGGCCTGGGCGCTAAGATCCCGCTCGCACACAACGACAAGAATGTGGTGAGCGCTATCGGCTCCATGGACTTCAACAACAAGCTGCAGCCTGCTTCCAAGGGCTTCGGTCTGGCTCTGGACAACGTCAACGGGCACGGACTGACGGTGATGAAGGAAAGTATCCCCGGGTTCGGGGACAGGCTGTCGGGCGCTGGCAAGCTGAACGTGTTCCACAACGACAACCACAACGTGGCCCTGACCGGCTCTCTTGCCAGGAACATGCCCAGCATCCCGAACGTGCCCAACTTCAACACGTACGGCGGGGGCGTCGACTACATGTACAAGAACAAGGTGGGAGCGACTCTGGGCATGGCCAGTACTCCGTTCTTGGACCGCAAGGACTACTCCGCGATGGGCAACCTGAACCTGTTCCGCAGCCCGACCACTACCGTGGACTTCAGCGGCGGCTTCAAGAAGTTCGAATCTCCCTTCATGAGCAGCGGCTGGAAGCCTAACTTCTCCTTTAATCTTGGCAGGTCATTCTAGAAAS. frugiperda- Chitin synthase B (SfChsB); AY52599) (SEQ ID NO: 105)ATGGCGAGACCAAGACCTTATGGTTTTAGGGCTTTAGATGAGGAGAGTGATGACAATTCGGAGTTGACTCCGTTGCACGATGATAATGATGACCTAGGACAAAGAACAGCTCAAGAGGCAAAAGGATGGAATCTGTTTCGAGAGATTCCGGTGAAGAAGGAGAGTGGGTCTATGGCCTCAACTGCCGGGATAGACTTCAGTGTAAAGATCCTTAAAGTCCTGGCGTATATTTTTATATTTGGCATAGTGCTCGGATCTGCGGTTGTGTCTAAGGGTACGCTGCTTTTTATCACATCACAACTGAAAAAGGGCAAAGCAATCGTTCACTGTAATAGACAGTTAGAACTGGACAAGCAGTTTATAACAATCCATTCGTTGCAAGAGCGTGTGACGTGGCTATGGGCAGCCTTCATAGCATTCAGTATTCCAGAAGTTGGCGTTTTCTTGAGATCAGTCAGAATATGCTTCTTCAAAACAGCACCGAAGCCTTCTGTTTTACAGTTTTTGACGGCCTTCGTAGTAGACACCCTTCATACAATAGGCATTGGATTACTGGTGCTTTTCATCCTGCCAGAATTAGACGTGGTTAAAGGAACAATGCTAATGAATGCTATGTGCTTCATGCCTGGAATACTAAACGCTGTGACCAGAGACCGCACGGACTCTCGATACATGTTGAAAATGGCACTAGATGTACTAGCTATCTCCGCTCAAGCCACCGCGTTCGTCGTCTGGCCTCTGCTAAAAGGCGTTAGTATGCTCTGGACGATTCCTGTCGCATGCGTATTCATCTCACTCGGATGGTGGGAAAATTTCGTCGGCGATATCGGAAAACAATGGCCAGTCCTGGAACCTGTACAAGAACTTCGTGACAATTTAAAGAAGACTCGTTACTACACACAGAGGGTGTTGTCTTTGTGGAAGATATTCATATTCATGTGTTGCATCCTGATATCTTTGGCGGCACAAGATGACAGCCCGCTTTCTTTCTTCACGGAGTTTGCTACTGGATTTGGTGAGCGCTTCTACAAAGTTCATGAGGTTCGAGCGATACAGGACGAATTTGAAGGTTTTCTGGGCTACAAAATTATGGACTTATACTTCGATCAAATGCCAGCGGCATGGGCCACCCCACTGTGGGTGGTGCTGATCCAGGTCCTGGCTTCTTTAGTCTGTTTTATGGCAAGTTTGTCTGCCTGCAAGATTCTGATACAAAACTTCAGCTTTACATTTGCGTTGAGTCTTGTTGGACCTGTCACCATCAACTTGTTGATTTGGCTTTGCGGCGAGAGGAACGCAGATCCCTGCGCATATAGTAATACGATACCAGATTATCTGTTCTTCGACATACCACCGGTGTATTTCCTGAAGGAGTTTGTGGTGAAAGAGATGTCGTGGATTTGGTTGCTGTGGCTGGTGTCGCAGGCGTGGGTGACGGCCCACAACTGGCGCTCCCGGGCCGAGCGTCTCGCCGCCAGCGACAAGCTCTTCAACAGGCCTTGGTACTGCAGCCCCGTCCTCGACGTCTCCATGCTGTTGAACAGAACCAAGAATGAAGAAGCGGAAATAACGATAGAGGATCTAAAAGAAACAGAGAGTGAGGGTGGGTCTATGATGAGCGGATTTGAAGCAAAGAAAGACATAAAGCCTTCTGACAACATTACGAGGATATATGTCTGCGCGACTATGTGGCACGAAACGAAAGAAGAAATGATGGACTTCTTGAAGTCTATCCTGCGTTTCGATGAGGATCAGAGCGCGCGTCGCGTCGCACAGAAGTACTTGGGCATTGTAGATCCTGATTACTATGAACTCGAAGTACACATCTTCATGGACGATGCTTTCGAAGTGTCGGACCACAGCGCGGACGACTCGAAAGTGAATCCCTTCGTGACGTGTCTCGTGGAGACTGTCGACGAGGCTGCTTCAGAGGTCCATCTCACCAACGTGAGGTTGAGGCCACCGAAGAAATTCCCCACACCGTACGGCGGCCGACTGGTCTGGACTCTCCCAGGAAAGAACAAAATGATATGCCACCTCAAAGACAAGTCCAAAATACGACACAGGAAAAGATGGTCTCAAGTGATGTACATGTACTACCTATTGGGCCACCGCCTGATGGACGTGCCGATCTCAGTGGACCGCAAGGAAGTCATCGCAGGGAACACCTACTTACTGGCTTTGGACGGCGACATTGACTTCAAACCGACAGCAGTCACGTTACTAATCGATTTGATGAAGAAGGATAAGAATTTAGGAGCAGCGTGCGGGCGCATCCATCCTGTGGGCTCAGGCTTCATGGCATGGTATCAAATGTTCGAGTACGCTATTGGTCATTGGCTGCAAAAGGCGACTGAACACATGATTGGCTGTGTACTCTGTAGCCCTGGATGCTTCTCCCTCTTCAGAGGAAAGGCTTTGATGGACGACAACGTTATGAAGAAATATACCTTAACTTCCCACGAGGCACGACACTATGTGCAATACGATCAAGGCGAGGACCGTTGGTGCACGCTACTGCTGCAGCGCGGGTACCGCGTGGAGTACAGCGCGGTGTCGGACGCGTACACGCACTGCCCCGAGCACTTCGACGAGTTCTTCAACCAGCGCCGCCGCTGGGTGCCCTCCACGCTCGCCAACATCTTCGACCTGCTCGGCAGCGCCAAGCTCACCGTCAAGTCCAACGACAACATCTCCACCCTCTATATAGTCTATCAGTTCATGTTGATAGTGGGTACGGTGTTGGGTCCCGGCACGATCTTCCTGATGATGGGGGGAGCCATGAACGCCATCATTCAGATCAGCAACGCGTACGCGATGATGTTGAACCTCGTACCACTCGTCATCTTCCTTATAGTCTGTATGACTTGTCAGTCAAAGACGCAGCTCTTCCTCGCTAACCTCATAACATGCGCATACGCAATGGTGATGATGATCGTGATAGTGGGGATAGTTCTGCAGATAGTGGAGGATGGATGGCTGGCTCCGTCCAGTATGTTCACAGCTTTAATATTCGGTACATTCTTCGTCACCGCGGCACTACACCCGCAAGAGATCAAATGTTTGTTGTTCATAGCAGTGTACTATGTAACCATCCCTAGTATGTACATGTTGTTGATCATATACTCCATCTGTAATCTCAACAACGTATCCTGGGGTACCAGGGAGACACCGCAGAAGAAAACTGCTAAGGAAATGGAAATGGAACAGAAGGAAGCAGAAGAAGCGAAGAAAAAAATGGAGAGTCAGGGTTTGAAGAAGTTGTTTGCCAAGGGAGAAGAGAAGAGTGGTTCGTTAGAGTTCAGTGTGGCGGGCCTGTTGCGATGTATGTGCTGCACCAATCCAGAGGATCATAAGGACGATCTCAACATGATGCAGATCTCACACGCGTTGGAGAAGATAAATAAGAGATTGGATCAACTCGATGTCCCTCCTGAGCCGACCCACCAGCCCTCGCATCCGCACACACACGTGGAGACGGTCGGTGTTCGTGATTACGAAGACAGCGAGATTTCCACTGAAATTCCTAAGGAAGAACGAGACGACCTGATTAACCCCTACTGGATCGAGGACGTGGAACTCCAGAAGGGCGAGGTAGACTTCCTCACCACCGCTGAGACCAACTTCTGGAAGGATGTCATCGATGAATACTTACTGCCTATTGATGAGGACAAGCGTGAAATTGAACGTATAAGAAAAGATTTGAAGAACTTGCGAGATAAGATGGTGTTTGCGTTCGTGATGTTGAACTCTCTGTTCGTGCTCGTCATCTTCCTGCTGCAGCTCAGCCAGGACCAGCTGCACTTCAAGTGGCCATTCGGACAGAAGTCCAGCATGGAGTACGATAATGATATGAATATGTTCATCATAACCCAAGACTACTTAACGCTGGAACCTATCGGTTTCGTGTTCCTCCTGTTCTTCGGCTCCATCATCATGATCCAGTTCACCGCCATGTTGTTCCATCGCCTGGACACGCTGGCCCATCTGCTGTCCACCACCAAGCTGGATTGGTATTTCAGTAAGAZGCCGGACGACCTATCAGACGATGCGCTAATAGACTCTTGGGCGTTGACAATAGCGAAGGATCTTCAACGTCTGAACACCGACGACTTGGATAAACGAAATAACAACGAACACGTGTCCAGGAGGAAGACCATATATAACTTGGAGAAAGGGAAGGAAACCAAACCGGCTGTTATCAACCTCGATGCCAACGCCAAGAGGAGATTGACTATCCTGCAGAATGAAGACTCAGAATTGATCTCCCGCCTGCCATCTCTGGGACCTAATTTGGCAACTCGTCGTGCCACGGTGCGTGCAATAAACACTCGACGCGCATCTGTCATGGCGGAGCGACGCAGGTCTCAGTTCCAAGCGCGACCTTCCGGGGGATCATACATGTATAATAACCCTCAAAACACGATTCAGCTGGACGATATGGTCGGGGGGCCGTCGACGTCGGGAGTGTACGTGAACCGAGGGTACGAGCCCGCCCTGGACAGCGACATCGAGGACACGCCCGTGCCCACCAGACGATCCGTTGTACACTTCACCGACCATTTCGCGTGATAACCACCAAAATCTGACTAACATCTCCATATTACATTTTCTACTCTGTTACGAAACGATAAAGTTTAAAGTGTATTAAATAAATTGGACAGATTTGAGTAGGTTTCACGTTTGTGTTTAAATAATTTATGAAAATAACATACCTATTGCTTTATGACCGCTTTAATATTAAAAGAAACTCAATATATGCATTAAT. castaneum- Peptidoglycan recognition protein LC (TcPGRPLC);DT786101.1 (SEQ ID NO: 106)ATATAAAAAAAAAAAAATATTAGGCAATTTATTTACACAAATAGCACAATTTATCAATTCACAAGTTGTGTATTTTATTATAAATACTAAATCAACAATAGCGACTAACAATTAACACAATTTTATTTCACTTCCTGTCACTATCGCGAGTAATAAATTCCCGCATCAAAATGCGGCCAATTTTTGATCTCTTTGTAAACATTTGGCCCCGGACTTTCCGTTCTAAACGTCTGATTGTGAGCTACCAGTTTATAATCCCTGGCCAATTTTCCACTCTTTACCCCCTCATCTAGCAATTTCTTCGCCACGCTGATCATCTCCGTCGTCAAATGATCATGAAAAAAATTCCCAATAAAACTAATCCCGATCGAATCATCCATGTGAAAATTCCTGATATCCCAGCCTCGTCCAACATACGCATTCCCATCTCCACCAATTACGAAATTGTACCCAATATCGGGACTTTTCAAATTGCCCACATGGTAGTCCTGCATGGACTGCACCCTTTGCGAACATGCAGGAAAGTCGCTACAAGTCGGGGTAACAGTGTGTGAAACGATGACAAAATGAGTGGGATGTGGCAGTGGTTTAGAGAAGTTTAAGGTGGCACGGCCTCCCCAAATTTTTTTCTCGATGATGGCACCGGGGCCCAAAGGAAGTCTTGGAGTCGCAGTGTTAGTGTCGGGAGTTTCGGGAGACTTTTCAGTTTTCCGTGGGAGTATTACAACGCAAGTTGTGGTGACGAGGATTACGATTACGACTAGAGAAATGCCCAAATATTTCACAGGTTTGGAGTATAAAAAGGAGTTTTTTTGGTTTTTCAGCGGGGGGGAGAGTCGGAAACAGGGGCTTTTCAGACTTGACGAAGTTTACCAATGGAATCAGTTGAATTAACAAATCCTTTTCCCTAAAGTCCTCTAAAAACAGATGATGGGGCCCCATT. castaneum- Peptidoglycan recognition protein 2 (TcPGRP2); XM_965754.3(SEQ ID NO: 107)ATGAGTGGCAGTGACCCTTTAACAAATACCCAACAATCCGATCAAGATTATTACCATCCACTCTGTTATTCAATTCAAGTGGACGACGAAAATGAACAATCAGCTCTCCTGCCCGCATTTCATCAAAGGAAAAGTTTGCGAGTTCAGGATAAAATCTTTATTGTATTTTTATTTTCAATTCTAATTACCGGACTAGCCATTGGCCTCTATCTCCTTGCAACTGAGGGACACGAATGGAAAGCTGCAGGAGTCTATAATATTACAGTTCGGGAACAGTGGCAAGCTCACGTCCCTTCATCAACAATGCCAAAGTTGGAACTTCCCGTAAGAAGAGTTTTATTTCTTCCTGCAAATACCACTAGCTGCGGCAGCAAATCCCACTGTGCCAAAGTCCTCCAGGAACTACAATTACAGCATATGCTGCAGTGGAAAGAACCTGACATCTCCTACAATTTCATAATGACTGCAGATGGCAGAATTTTCGAGGGGAGAGGATGGGACTTTGAAACTTCTGTTCAAAATTGTACGGTTAATGATACTGTGACAGTTGCTTTTTTGGACGAATTAGATGCGAAAGCACCGACGTTTAGACAAGCTGAAGCGGCAAAAATGTTCCTGGAAGTGGCAGTAACAGAGGGAAAATTAGAACGGTGTTTTAATACCGCGGTCTGGGGAGGAAATAAATTCTTCATTGATTTGGCTCGAAATGTTCAAGACGTCTTATCGGAATGCGAGGGAATTACTTAAT. castaneum- Beta 1-3 glucan binding protein (TcβGRP2); XM_966587.4(SEQ ID NO: 108)ATGTGCGTTTGCAAGACTGTGCTATTAATCGTTGGGCTGGGGGGTTGTTTTGCGGGTCCGGTCCGTAATTATGGGCCATTGAGACATTACAACGTTCCGCGACCCTCAATCCAAGCATTCAGGCCCCGTGGCTTCAAAGTGAGCATCCCTCATACTGAAGGCATCCAATTATTCGCCTTTCATGGAAATATTAATAAACCCCTGCACGGTCTCGAGGCCGGACAATTTTCCCAAGACGTCCTCCAAAGGGAGGGAGACGAGTGGGTGTTCCAAGACTCCAGTGCCAAATTAAACGTGGGAGATAAAATCTATTATTGGCTCTTTATCATTAAAGAAGACCTGGGCTACAGATACGATCACGGCGAGTACGAAGTGAAAGTTTTAGCCACCCGTGACTTCGATTCTCCTCAAACAACCTCTGTGACGCCGAATCTTGCCCCCAATCTCGGCATTTGCGAAAAGGTGATGGTAAATCTCACGCGGAAGCTGCTCGATTTGCAGCAAGAAATTGAGTCCCTTAGGGAGACGAACGATATTTTGGAGGATATGGTTCAGAAGCACACTGATACGGCGACTACTCTCACTTTGGACGGCTTGATGATAAAGGATGACGACGAACTTGTTTCGGTGATTCAAGCAATTATTAAAGATAAACTTGGACTAAAAAGCAGGATTCAAAATGTGACGAGGCAGGAGAATGGAATGGTCAAGTTTCAAGTGGCGAGTTTGAGAGAAAAGTTGGAGGTTGTCAAAGCGGCCAAAAGAAAACTCAAGTCGTCGAGCTTTACGATCACGTATTAA T. castaneum- midgut protein (TcMDGP); XM_971351(SEQ ID NO: 109)ATGTATCCGTTGAAGAGGATGCCCAGTGAAGAAATCAGTATCAGTGATCTGCCTAGCGAAATGAAAGAAGTTTTACTAGAAATTAGCCCGAACTTTGATGAAAATCTGAAACGGGCTTTCAGGAACGAAGGAGTGAGGCTGCAGAGAGTGCAGAACAATGGACGATTTATTCATCAGCTGGACGACGTTCTCTTATCCATAGACGATACCAAAATCGAGTTACGCAACCTGAAATTCCCCTGGATCCCCGACTTCCGCATCGTGGACTTGTCCAGCGACCTGCCCATGTCATGCCTCGACCTAAACCTAAACCTGGGCAATTTGCGAATTGAGGGCGAGTACGAAGCCAACAACACCACACTCAGGCGATGGCTCCCGGTATCTCACATTGGTCGAATCGTGATCGGTTTTAACAACGTCCGAGCGAACGGAAAAGTCGGACTCGTGCTCGAGCAGGATTCTTTCGTTCCGCAGAATTATGATATTAGATATGAGCCGACGGATGTTGTTATTAGGGTTAGCTATCACGTGGATGGCGAGAATGAGGTGCAAAATGAGATTAGCAATTCAGATATTGAGGCCACGCTAGGCAAGACCGTGTGGGTGCAGTTGACTGAGATATTGTCCAACCTGTTGCATAGGCAATTGGGCGAGGTTGTAGTGGAGTTTTCCGTGACGGAACTCCTCGTCGATAGGGACGAGGAATACAGGGAATACGCCAAGGGACAAGCAGCGCGCGCCAATAAACTCCTGGACTCGCTTTTGTGCTCAGCCAAGGACTATTTAGTCGCGAAGGACTTGAGGACGGTCAAAACGCCACCCTTCGACGTCGTCTTCAAAGGGAAAGTCTCGGGGGTGCAGCAGGGGACCTTCAGCACGGGGGAAGGGTTCCTCCAAGACCTCGCCACTTTGACGCGGAGACACAGCTTTAGTTTGTACGAACACAAACACAAACTCACGATATATGGGGGGATCAGGCTGAGGGAGTTTAAACTCGGGTATGGGGGCTACCAGGGCCAGTACGAGGAAACGGCCGTCTCAGGCAGCATCAAAGGCTCGCTCTACAAGAACGAGATTTTCGTCAAGATCACTGTGAAAAAAGAAGGCGAGCGGTGCTCGACTCAGTTAGATTCCGTCCAAGTTGTTGTTGTAAAGTAA T. castaneum- Chitin synthase 2 (TcCHS2); EFA 10719.1(SEQ ID NO: 110)ATGGCGGCGCGTCATCGCTTTGCCACAGGGAGCCCTGAGGAAACAGAGCCCCTGTATTCGTCGACGCAAATGCCCGAAAAAGTCCGGGAAAAATGGAACGTCTTCGACGACCCCCCAAGAGAGCCCACTTCGGTTCCGAAGTCAAAAGAACCTACATCGAGTGGGGGGTGAAGTTTTTGAAAGTTGTGACAATCATAACTGTGTTTTTTGTGGTCCTTGGTGCTGCAGTGGTTTCGAAAGGGACAACCTTGTTCATGACGTCACAAATAAAAAAGAATGTGACAAGGGCTTATTGCAACAAAAAGATAGACCGCAACCTCCAATTCGTCGTCTCCCTCCCCGAAGTGGAGCGCGTGCAATGGATCTGGCTCCTCATTTTCGCTTACTTGATCCCCGAAGTGGGTACCTGGATCCGCGCCGTCCGCAAATGCCTCTACAAGCTCTGGAAAATGCCCTCCCTCTCCGAATTCCTCTCCCTCTTCGGCACGGAAACGTGCCCCGCCATCGGAAGCGCAATTTTGATATTCGTCGTCCTCCCCGAGCTGGACGTCGTCAAAGGGGCGATGCTCACAAACGCGGTTTGTTTCGTGCCCGGAGTTGTGGCAATGTTCTCGCGCAAACCGTGCTCCATAAACGAGAACCTGAAAATGGGGCTGGACATCGCCAGCATAACTGCACAGGCGTCAAGCTTCGTGGTGTGGCCCTTGGTTGAAAATAACCCGACCTTGTACCTAATCCCCGTTTCCGTGATTTTGATTTCGGTGGGTTGGTGGGAGAATTTCGTGTCGGAAACGTCCTACTTACCGTTTATCCGGGCTCTGGGCAAGAGCAAAAAGGAGTTTAAGACAAGGACGTACTTCATTTACGCGTTCGTGGCCCCGGTTAAATGCCTGGCGTTTTTTTGCACCGCTTTGGTCATTTTTTACTGCCAGGAGGGCAGTGTTGACTTTTTATTTGATAATTTTTCAGCCGCGTTTCAGGATCATAACATTGAAATTACCGAAGTCGCGCCCGTCTTGCCGGGGAATTACGCAAATGCAGTTCGGTCGGGAGCCGAAACCCCATCCACACAAGCAGTTACATGACGGGGATTTGGGTTTGGTTGATTAACATTTCGGCGACTTATATTTGCTACGCGTTTGGGAAATTCTCCTGCAAAGTCATGATCCAGAGCGTTAGCTTCGCTTTTCCGATCAATTTGTCGGTCCCTGTCCTCTTATCCGGACTGATCGCAATGTGTGGCATGTACTACAGGGATGAGTGTTCTTTCGCTGAGTCAATTCCTCCATATTTGTTTTTCGTTCCTCCACCTCTCATGTTCCTACAAGATTTTCTCTCGCACCAACACGCCTGGATTTGGCTGGTTTGGTTGCTGTCACAAGCCTGGATTTCGGTGCACATTTGGTCCCCAAACTGTGACAAACTTTCAAGCACCGAACAGTTGTTTATTCGGCCCATGTATGACGCGTTTTTGATCGATCAAAGCCTGGCGTTAAACCGGAGACGTGACGAAAATCCCAGAAATTACAGAAGCGACGAAGGGCCTCAAATTACAGAGCTCGAGCCGGAAACGATCGAGAGTCAGGACGCCATAACCCGGATTTACGCCTGCGGGACAATGTGGCACGAAACTCCCGAAGAAATGATGGAATTTTTGAAATCGGTGTTCCGCTTGGACCAAGACCAGTGTTCCCACAGGATTGTGAGGGAGCATTTGGGACTCAAGCATGACAATTACTACGAATTGGAGACTCATATATTTTTCGATGATGCGTTTATTCGGACCAGTGAAGACGATAATGATCCCCACGTCAACGAATACGTTGAGTCACTTGCGTCCATTATCGACGAGGCTGCGACTAAGGTTCACGGTACCACGGTGAGGGTGCGTCCGCCCAAAGTGTACCCCACGCCTTACGGCGGACGCCTGGTCTGGACGCTCCCAGGGAAAACAAAAATGATCGCCCACTTGAAGGACAAGAAGAAGATTAGGGCGAAAAAGCGCTGGTCTCAGTGCATGTACATGTACTTTTTGCTCGGATTCAGATTGCAAGCCAACGACGAACTCTCCGCCCACAGCAAGGAAATCCGCGGCGAAAACACCTACATCCTCGCCCTGGACGGCGACATCGATTTCCAACCCGAAGCCCTGCACCTCTTGGTGGACTACATGAAGAAGAACAAAACGTTGGGGGCGGCCTGCGGCCGCATCCACCCCATCGGCAGCGGCGGCATGGTCTGGTACCAAATGTTCGAATACGCCGTCGGTCACTGGATGCAAAAAGCCACCGAGCACGTCATAGGCTGCGTCCTCTGCAGCCCCGGCTGTTTCTCCCTGTTCCGGGGAAAAGCCCTCATGGACAAAAGCGTCATGAAGAAGTACACCACTCGATCGACCCAAGCCAAGCACTACGTGCAGTACGATCAAGGGGAGGACCGGTGGTTGTGCACTTTGTTACTCCAGAGGGGCTACCGTGTGGAATACTCCGCAGCCTCGGACGCTTTCACGCACTGTCCGGAAGGCTTCAACGAGTTTTACAACCAGCGGAGGCGCTGGATGCCGTCCACTATGGCCAACATTTTGGACCTTTTGATGGATTACGAGCACACGGTCAAAATCAACGAAAATATTTCCATGCTGTACATCGGGTACCAAATTATTTTAATGATCGGTACGGTCATTGGCCCCGGTACTATTTTCCTCATGTTGGTCGGCGCCTTCGTGGCTGCCTTTGGGCTCGACCAATGGAGCAGTTTCTACTGGAATTTACTACCAATCGCAGTTTTTATCCTAGTATGTGCCACTTGTAGCTCCGATATCCAATTATTTTTCGCCGGCCTTATCAGCGCCATTTACGGCCTGATAATGATGGCTGTTTTCGTCGGTGTGATGCTCCAAATCAGCCAAGACGGCCCACTTGCGCCTTCCTCCCTTTTCTTCTTCTGCATGGCTGCTGAATTTATAATCGCAGCACTGCTGCATCCGCAAGAATTCAACTGTTTGAAATACGGGGTCATTTACTACGTCACGGTCCCCAGCATGTACCTCCTCCTAGTCATCTACTCGGTCTTCAATATGAACAACGTGTCCTGGGGGACGCGCGAAGTGACAGTCGTGCCCAAGCCTGACCCCAACGCCGTCCAGAAAATCGAAGAGAAAAAACCGGAGAAGAAAGACAAAGTTTTGACGTTTCTGGGCGCGAATGCCCAGGACGACGAAGGCGGGCTTGAATTTTCGGTCAACAAACTTTTCAAATGCATGATTTGTACGTACAAGGCCGATAACAAGGAAAACGAGCAGTTGAGGAAAATACAAGAGTCGTTGAGAGACTTGAATAGGAAAATCGAGTCGCTGGAAAAAATGCAATATCCGGATTTGAGGTCTCCTGCCGTTAGCAACGTTACAACGTTCATGGAGGGCTCAAAGGCGACGGTTAAGAACAACGTGGAGGATAACTACATGGAGGCTCCGCAAGACAATGTTTCGCAACCGTCGGATGAGGTCATGGAGAATAGTTGGTTCTACGATGGGCCTTTGATTAGGGGGGAAGTGCATTACTTGAATAGGAATGAGGAAACGTTTTGGAATGAACTGATAGAGCAGTATTTACACCCGATTGAGGATGACAAGAAGAAGGTTTCGGCTGAATTGAAAGATTTGAGGGACAAAATGGTGTTTACTTTCCTGATGTTGAATTCGCTCTACGTTATTGTGATTTTTTTGCTCACTTTGAAGAAGGATTTGCTCCATCTGGACTGGCCGTTTGACCCCAAAGTGAACTTCACGTATTTCGAGGACAAAAATGAGATTGGCGTTTACATAACATACCTCCAGCTGGAGCCCATCGGTTTCGTGTTCCTCATATTTTTCGCCCTGCTTATGGTGATCCAGTTCTTCGCAATGATGATCCACCGCTTCGGCACCTTCTCCCAAATCATCACAAAAACACAATTAGACTTCGATCTGTGCAGCAAACCAATCGACGAAATGACTGTGGACGAACTCAGGTCCCGCGACCCTATAAAAATAGTCGCAGATTTACAAAAACTAAAAGGTATAAACAACGAATACGAGGACCAAACGGAAGTTCCGGTCGAAATGCGAAAAACAGTAAGTAATTTGGCGCAAACGAGCGGTGGTGGTGAGAACAAGCCCATATTTTATTTGGATGAAGCGTTCCAAAGGCGAGTCACTCAAATAGGGAGCACTTCAAGCAACAACCCGAGCATTAGCGCCTTTAGGAAGAAAAGCCTTGCTTACGTCCAACAAAGAATGAGCATAGCCCCAAATCGGGTGTCACAAGCCCGGCCTAGTGTGCAGTTACGGTACCCCAATGGAAAAGCCAACGAAAATTTCGTTTTTGACGAAAACGGGTCAGACGTGGAGGCATAA> M. sexta-Peptidoglycan recognition protein 2 (MsPGRP2); GQ293365.1(SEQ ID NO: 111)TAATCATTAGAAAAATGGCGAGCTTCGCTTTAATAGTTATCCTTAGCGTAATTGGCTTTATATCGGCCTATCCTAGTCCTGAAGGTTACAGTTCTGCCTTCAACTTTCCATTCGTAACCAAGGAGCAGTGGGGCGGCAGGGAGGCACGCACGTCGACGCCACTCAACCACCCAGTGCAGTTCGTGGTGATCCACCACAGTTACATTCCCGGCGTGTGCCTCAGCCGGGACGAGTGCGCGCGCAGCATGCGCTCCATGCAGAACTTCCACATGAACAGTAACGGGTGGAGTGATATTGGATACAACTTCGCTGTCGGCGGTGAAGGGTCGGTGTACGAGGGCCGCGGCTGGGACGCGGTCGGCGCACACGCAGCTGGCTATAACAGTAACAGTATCGGCATCGTGCTCATCGGCGATTTTGTTTCAAACCTCCCGCCGGCGGTGCAAATGCAAACCACACAAGAATTGATCGCAGCGGGCGTGCGACTCGGTTACATCAGGCCCAACTACATGCTCATCGGGCATCGTCAGGTCTCCGCCACTGAGTGCCCAGGAACCAGACTCTTCAACGAAATCACCAACTGGAACAACTTCGTGAGG> M. sexta-Beta-1, 3-glucan-recognition protein 2 (MsβGRP2); AY135522.1(SEQ ID NO: 112)GAGCGTCTGTTTGTTCGCAACCATTGCGGGCTGCTTGGGCCAGCGAGGGGGTCCATACAAGGTGCCTGATGCGAAACTCGAAGCTATCTACCCCAAAGGCTTGAGAGTCTCTGTGCCAGATGATGGCTACTCCCTATTTGCCTTCCACGGCAAGCTCAATGAGGAGATGGAAGGTTTAGAGGCTGGCCATTGGTCCAGAGACATCACCAAAGCGAAGCAGGGCAGATGGATATTCAGAGATAGGAATGCTGAGCTGAAGCTTGGAGACAAAATTTACTTCTGGACTTACGTTATTAAGGATGGATTGGGATACAGGCAGGACAATGGAGAATGGACTGTTACAGAATTCGTCAATGAGAACGGTACAGTGGTGGACACTAGTACAGCGCCGCCACCAGTAGCACCCGCCGTTTCAGAGGAAGATCAATCGCCAGGTCCTCAGTGGAGACCTTGCGAAAGATCCCTGACTGAGTCCTTGGCCCGCGAACGCGTTTGCAAAGGCAGCCTTGTCTTTAGCGAGGACTTTGATGGTTCCAGTTTGGCCGACTTGGGCAATTGGACCGCTGAAGTCAGATTCCCTGGCGAACCGGACTACCCGTACAACTTGTACACTACGGACGGCACTGTGGGATTCGAAAGTGGGTCTCTGGTGGTGAGACCCGTCATGACCGAGTCCAAATACCACGAGGGCATCATATACGACCGCCTCGACCTTGAGAGATGTACAGGACAGCTGGGTACGCTGGAATGCAGGCGAGAGAGCAGCGGCGGTCAGATTGTACCACCTGTGATGACAGCTAAACTGGCCACTCGACGCAGCTTCGCGTTCAAGTTCGGCAGGATCGATATAAAGGCGAAGATGCCGCGCGGGGACTGGTTGATACCAGAACTCAACCTCGAACCTTTAGATAACATATACGGCAACCAGCGATACGCTTCGGGTCTCATGCGGGTCGCGTTCGTGAGAGGAAACGATGTATACGCCAAGAAGCTCTACGGAGGTCCGATAATGTCCGACGCGGACCCGTTCAGGTCCATGCTGTTGAAGGACAAGCAAGGGTTGGCCAACTGGAATAATGATTACCACGTCTACTCGCTGCTGTGGAAGCCTAACGGTTTAGAGCTGATGGTGGACGGTGAAGTGTACGGCACCATCGACGCTGGCGATGGCTTCTACCAGATTGCGAAGAACAACCTCGTGAGCCACGCCTCGCAGTGGCTCAAGGGCACCGTCATGGCGCCGTTTGATGAAAAGTTCTTCATCACTCTGGGTCTTCGCGTGGCGGGTATCCACGACTTCACGGACGGTCCGGGCAAACCTTGGGAGAACAAGGG> M. sexta-Relish family protein 2A (MsREL2A); HM363513.1(SEQ ID NO: 113)ATGTCCTCTTGTCCAAGCGACTATGATCCCAGTGAATCGTCCAAATCTCCACAAAGTATTTGGGAGTCAGGAGGATACAGTTCTCCGTCGCAACAAGTTCCTCAATTGACTTCTAACTTAACAGAATTGTCTGTTGATCACAGCTATAGATACAATGGAAATGGACCATATCTACAGATCACAGAGCAACCACAGAAATACTTTCGGTTCCGTTATGTTAGCGAGATGGTGGGAACACATGGATGTTTGCTTGGCAAATCTTATACAACAAACAAAGTTAAAACTCATCCGACAGTTGAACTCGTGAATTACACCGGTCGAGCCCTGATAAAGTGCCAACTATCGCAAAACAAGAGCGAAGACGAACACCCGCACAAACTGCTCGATGAACAAGACAGAGACATGAGCCACCACGTTCCCGAGCACGGCAGTTATAGAGTGGTATTTGCTGGTATGGGTATAATTCATGCTGCCAAAAAGGAAGTTGCGGGGTGGCTCTATAGAAAATATATACAGCAGAACAAGAATGAAAAGTTTAATAAGAAAGAGCTCGAAGCGCATTGTGAGAGGATGTCCAAAGAGATCGATTTAAATATAGTTAGACTGAAGTTTAGCGCTCACGATATTGACACTGGCATTGAAATTTGCCGGCCAGTGTTCTCTGAACCCATTTATAATTTGAAGTGTGCGTCTACGAATGATTTGAAAATATGCCGCATAAGCCGTTGTTACGGTAGACCGAGAGGCGGCGAAGATATCTTCATATTTGTCGAAAAGGTCAACAAGAAAAACATCCAAGTTCGGTTCTTTAGACTGGAAAACGGGGAGCGCACCTGGTCAGCGATGGCGAACTTTCTGCTAAGCGATGTTCACCACCAATACGCTATCGCTTTTAGAACGCCACCGTACGTCAATCACCAAATTTCTGAAGACGTGCAAGTTTTTATAGAACTCGTACGCCCTTCAGACGGTAGGACGAGCGCTCCCATGGAGTTCACATACAAGGCTGAGCAAATCTATAAACAGAACAAGAAACGTAAAACTACTTCGTCGTACTCGTCGCTCGACAGCTCCTCAGGTTCGGCCGGTTCAATTAAAAGCATCAGCGAACTGCCCGCGCCCGTTGTTTTTGCTGAAAACGTAAGTTTTTTCTATGACACATTACTCATTCTTCAACCCATGACGAATCTATAA> M. sexta-Spätzle (MsSPZ1A); GQ249944.1 (SEQ ID NO: 114)ACGAACAACCTGACAGACGGATAGCGGGACGGTCAGCACAATACGAACATTTAAGAACAAACGAGAGGTCTCTCCCGGTCTACAGCGAGACCCAGAGGATACAAGCAGAAGAGAGAAGAAGACACAGTTCGAGACTAGAAGAACCGAGACAACGTGCTGAGAATGGTTCATATAAGATATTGAATAACCCTCCGAAACCCTGTATTACTAATAGGAGAAGTCAAATTGATTCGTCGAATGATAGGGTAGTGTTCCCCGGTCCGACTTCAGAAAGGTCGTACGTACCCGAAGTGCCAGAGGAATGCAAGAAAATCGGCATATGCGACAGTATACCGAATTACCCAGAAGAACACGTAGCTAATATTATATCTCGACTTGGAGACAAAGGAAAAGTATTACAAATAGACGAACTGGACGTATCAGACACTCCAGATATCGCCCAGAGGTTGGGTCCGCAGGAGGACAACATGGAACTATGTAGCTTTAGAGAAAAGATTTTTTACCCCAAGGCAGCGCCAGACAAAGATGGAAATTGGTTCTTCGTTGTGAATTCAAAAGAAAACCCAGTACAGGGTTATAAAGTTGAAATTTGCGACCGTCAGCAATTACCATGCGCGGAGTTCGCGAGCTTCCAACAGGGATATGAAGCGAGGTGCATCCAGAAATACGTTCGCCGGACCATGTTGGCGTTGGATCCCAAGGGTCAGATGACCGACATGCCCCTTAAAGTGCCCAGCTGTTGCTCATGCGTGGCCAAATTGAC> M. sexta-Toll receptor (MsTOLL); EF442782.1 (SEQ ID NO: 115)ATGCAGGCTCGGCGGTGGTGCGCGGCACTGCTATTAATGCAGATGCTGAGCTGGCTCGGAGTCAGTGGACACTTACCGCGTCCCGAGTGCGCGCCAGCCGCAGATTGCCAACTTATACGAGACAACATAATCGATGGATATGCACAATTCTACTTCAACGTATCAGGACATGAAGTGAAATTTGAACATTACATCGGAAACGACTTCGATGTCGAATTGTCATGCAATTACATCGCCATGGACAACGCAATGCTGCCGCGGTTCTCAACGACCTTTTCAGTCAACGTAATAGTGGTTAAAGAATGTGCTTTGCCAAGAAGTGGGTCAATCGATGCCGCTGTCGCTGCACTTAATATCAACGTTTTGACGGAGCTGACTCTGGACAAATTCCTAGAGCCGGCGGTGATCACGCGCGCACATCTTACCAGTTTACAACGACTAGAGAGGCTGGAGCTACACGGTAACTCAAACACAAGCCTCGCCCCCGGCGCACTGGCCGCGCTCTCCGCCGCGTCCGCACTGAAATGTCTTGTATTGCATGCAGTACGCGTGCCCGCCGCTGACCTGGCGCGCTTGCCGTCGTCACTGCAAGAACTAGCGTTGTTGGATGTGGGCGCTGCGAGTATGCATTTAGATTCATCGGTTAATTTGACGTCACTCTTCGTAATCGATACACATTATCCTGTCGTCGTGAATGTGAGCAACGCCGTTGCGCTCAGAGACTTGCACATAAATACCCCAAGTACTGTGTTGACCGAAGACGTGCTCCCGTCGTCACTCAACTCACTTGAACTAGAGGGGTGGAACGAAACGCATCCGGTGCCTAAGACACGTTGTGTACTACTTAAGGAACTTAATGTAATCGGCACCGACAATGATGCCTATCCGGTGACTCT> M. sexta-Valine Rich Midgut Protein (MsVMP1); NCBI accession numbernot assigned as yet (SEQ ID NO: 116)ACGGACCTTCCGTTGGACCNGCCATCATCGGCGCTGGAGACATCGCTGTCGGCCCTGCTATCGTCGACTTCCCTTTCCCCGACGGCGGTGCCGTGTCTGCCCCCGTTGAGCCTTCCCCCATCGCCATCGGACCCGCTATCGTCGGTGAATCCCCTATCTCCGTCGGACCTGCCATCGTTGAGGCCGGAGACATCGCTGTTGGACCCGCTATCATCGACTTCCCCCTTCCCGACGGTGGCGCCGTGTCCGCCCCCGTTGAGGTTTCTCCCGTCGACTCCGTCGTCGTCGGCCCTGCCGCCGGCTCTCAGAGCTCTCCCCTCGTCCAGATCATCATCAACGTTAAGGCCCCCGCTGGTGCCGGCCCCGTTGTCGATGCCGTCGCTGACAAGCCCATGGACATCATTGATGTTATGCCCGTCGTCGACCCTGCTGATTTCGTGGACCTCACCCCCGTTGTAGAGCCTGTAGAAGTCGTCGACATTGTCGATGTCATGCCCGTGGTTGACCCCATCAACATCATCGATGTTATGCCTGTTGTTAAGCCCGTAAACCCCCTTGCCCGTTCTT> M. sexta-Chitin synthase 2 (MSCHS2); AY821560.1 (SEQ ID NO: 117)ATGGCCGCAACTACACCAGGTTTTAAGAAGTTAGCAGACGATTCTGAGGATTCAGATACAGAATACACCCCGCTGTATGATGACGGTGATGAAATAGATCAAAGAACTGCACAAGAAACAAAAGGATGGAATCTATTTCGAGAGATTCCGGTGAAAAAGGAGAGTGGATCTATGGCCACAAAAAATTGGATAGAAACAAGCGTAAAAATCATAAAAGTGCTTGCCTACATATTGGTTTTTTGTGCTGTACTGGGTTCCGCAGTCATAGCTAAAGGAACTCTTCTATTTATTACGTCACAACTGAAGAAAGACAGACAAATTACTCACTGCAATAGACGACTTGCTTTAGACCAACAGTTCATAACGGTACACAGTTTAGAAGAAAGAATAACATGGCTATGGGCAGCACTTATTGTATTCGGTGTGCCGGAGTTAGGGGTGTTTTTGAGATCCGTCAGGATATGCTTCTTCAAAACTGCCAAGAAACCAACCAAAACACAGTTTATTATTGCTTTCATAACAGAGACACTACAAGCAATAGGAATAGCAGCACTTGTATTAATAATTCTACCAGAATTAGACGCTGTGAAAGGAGCCATGTTGATGAACGCCACGTGCGCTATCCCTGCATTGCTAAACATTTTCACGAGAGACCGAATGGATTCTAAGTTTTCTATAAAATTGATATTGGATGTATTGGCGATATCGGCACAAGCCACGGCGTTTGTTGTTTGGCCTCTTATGGAAAGAACGCCAGTTCTATGGACCATACCAGTTGCATGTGTGTTAGTGTCTCTAGGCTTCTGGGAGAATTTTGTTGACACCTACAATAAAAGTTATGTTTTTACGGTGCTGCAGGAACTACGCGACAACCTCAAGAGGACTCGGTACTACACTCAGCGGGTGCTATCTGTTTGGAAGATTATAGTGTTTATGGCATGCATTTTAATATCGCTGCATATGCAAAATGACAATCCGTTTACCTTTTTCACTCACGCCAGCAAAGCCTTTGGAGAGAGACAGTATGTCGTTAACGAGGTTCTAATAGTAGTCCGAGATGACGAAACCATAGGCTATGACGTCACCGGAGGTATATTCGAATTGGACGCGATATGGACCTCAGCATTGTGGGTCGCATTAATTCAAGTGGGAGCAGCCTACTTCTGTTTCGGAAGTGGCAAGTTTGCTTGCAAAATTCTTATACAAAATTTTAGTTTCACTTTAGCATTGACTCTCGTCGGGCCCGTGGCAATCAACCTCCTTATTGCTTTCTGCGGAATGAGAAATGCAGACCCTTGCGCTTTCCATAGAACTATACCTGACAATTTGTTTTACGAGATACCACCTGTG> M. sexta-Beta-fructofuranosidase 1 (MsSuc1); GQ293363.1(SEQ ID NO: 118)TTGCTGTGCGTTTTCCTTGGTAGTGTATCGTCATGTTGCGTTAATGGGCGGTACTACCCGAGGTACCATTTGTCGCCACCGCATGGCTGGATGAACGACCCCAACGGATTCTGCTACTTCAAAGGTGAATACCATATGTTTTACCAGTACAATCCCATGTCAAGTTTGGAGGCTGGCATAGCTCATTGGGGTCATGCGAAAAGTAAAGATTTGTGCCATTGGAAACACTTAGACCCGCCATCTATCCTGATCAGTGGTACGATCAAACGGGAGTATTTTCTGGAAGTGCGCTAGTAGAGAATGACGTCATGTACCTTTATTATACTGGAAATGTAAATCTTACTGATGAAATGCCATTTGAGGGACAATTCCAAGCTCTTGGTATCAGTACTGACGGTGTCCACGTAGAAAAGTATAAAGACAATCCAATAATGTACACGCCAAACCATCAACCTCACATCCGAGACCCAAAAGTTTGGGAACACGACGGCTCTTATTATATGGTCTTAGGAAACGCATATGATGATTATACAAAGGGCCAAATAGTTATGTACGAATCATCAGACAAGATCAACTGGCAAGAAGTAACTATACTATATAAATCAAATGGATCTTTCGGTTACATGTGGGAGTGTCCAGATTTATTCGAAATAGACGGCAAGTTTGTACTTCTGTTCTCTCCTCAAGGCGTGAAGTCTGTGGGCGATATGTACCAGAATCTGTATCAAGCAGGATACATCGTCGGAGAATTCGATTACGATACTCATTCATTCACAATACTAACCGAATTCAGAGAATTGGATCACGGTCATGATTTTTACGCTACACAAACAATGAAAGATCCTAGTGGAAGAAGAATAGTCGTTGCTTGGGCAAGT> M. sexta- Beta-1 tubulin (MsβTub); AF030547 (SEQ ID NO: 119)ATGAGGGAAATCGTGCACATCCAGGCTGGCCAATGCGGCAACCAGATCGGAGCTAAGTTCTGGGAGATCATCTCTGACGAGCATGGCATCGACCCCACCGGCGCTTACCATGGCGACTCGGACCTGCAGCTGGAGCGCATCAACGTGTACTACAATGAGGCCTCCGGCGGCAAGTACGTGCCGCGCGCCATCCTCGTGGACCTCGAGCCCGGCACCATGGACTCTGTCCGCTCCGGACCTTTCGGACAGATCTTCCGCCCGGACAACTTCGTCTTCGGACAGTCCGGCGCCGGTAACAACTGGGCCAAGGGACACTACACAGAGGGCGCCGAGCTTGTCGACTCGGTCTTAGACGTCGTACGTAAGGAAGCAGAATCATGCGACTGCCTCCAGGGATTCCAACTCACACACTCGCTCGGCGGCGGTACCGGTTCCGGAATGGGCACCCTCCTTATCTCCAAAATCAGGGAAGAATACCCCGACAGAATTATGAACACATATTCAGTTGTACCATCACCCAAAGTGTCTGATACAGTAGTAGAACCTTACAATGCAACACTGTCAGTCCACCAACTCGTAGAAAACACCGACGAAACCTACTGTATCGACAATGAGGCTCTCTATGACATCTGCTTCCGCACGCTCAAACTTTCCACACCCACATATGGCGACCTTAACCACCTGGTGTCGCTCACAATGTCCGGCGTGACCACCTGCCTCAGGTTCCCCGGTCAGCTGAATGCGGATCTCCGCAAGCTGGCGGTGAACATGGTGCCCTTCCCGCGTCTGCACTTCTTCATGCCGGGCTTCGCTCCGCTCACGTCGCGCGGCAGCCAGCAGTACCGCGCCCTCACCGTGCCCGAACTCACCCAGCAGATGTTCGACGCTAAGAACATGATGGCGGCGTGCGACCCGCGTCACGGCCGCTACCTCACCGTCGCCGCCATCTTCCGTGGTCGCATGTCCATGAAGGAGGTCGACGAGCAGATGCTCAACATCCAGAACAAGAACTCGTCGTACTTCGTTGAATGGATCCCCAACAACGTGAAGACCGCCGTGTGCGACATCCCGCCCCGTGGTCTCAAGATGTCGGCCACTTTCATCGGCAACTCCACCGCTATCCAGGAGCTGTTCAAGCGCATCTCTGAACAGTTCACCGCTATGTTCAGGCGCAAGGCTTTCTTGCATTGGTACACCGGCGAGGGCATGGACGAGATGGAGTTCACCGAGGCCGAGAGCAACATGAACGACCTGGTGTCCGAGTACCAACAGTACCAGGAGGCCACCGCCGACGAGGACGCCGAGTTCGACGAGGAGCAAGAGCAGGAGATCG> P. xylostella- Peptidoglycan recognition protein 2 (PxPGRP2);ACB32179.1 (SEQ ID NO: 120)CCCGATACAGTTGGAGTACCTGCCCCGGCCCCTGGGGCTGGTGGTGGTCCAGCACACCGCCACCCCCGCGTGTGACACTGACGCCGCGTGTGTGGAGCTGGTGCAGAACATACAGACCAATCATATGGATGTGCTGAAGTTTTGGGATATTGGACCGAACTTCCTGATTGGTGGGAACGGCAAGGTGTACGAGGGCCCTGGTTGGCTGCACGTCGGCGCCCACACTTACGGCTACAACAGGAAGTCTATCGGGATCTCTTTCATTAGGAATTTTAATGCTAAGACCCCAACAAAAGCAGCGTTGAATGCGGCTGAAGCATTGCTGAAGTGTGGAGTGAGAGAAGGACACCTGTCTCACTCATACGCAGTGGTCGGCCATAGACAACTGATCGCAACAGAGAGCCCAGGCAGGAAACTGTACCAAATCATCAGGCGCTGG> P. xylostella- Immune Deficiency Protein (PxIMD); Px003008(SEQ ID NO: 121)TCCCGAAGCCACCTAGAGAACCGGTAGAGACTACAGAGACATCACAAAATAATACCGAAAATCAACCTTACAATGTCGAAGAAGAGGAAATACCCGAACCAGAAAAGCCTAAGAAAGAAAAAAAGAATCCCAAGCCTACCAAAAAAACTTTCTTTAATCGTGACAAAACTAACAAACACGACGATACCCGCAAACATACAAAATCCGGAAAGGACCAGACATCAATTAATACTCAAGGTAACTTGAAAATTATACTTCCTGTAACTTAAACGGCCCGTTGACCCCAGTTTACCTTTCGCCTTTCTTGATATATTTTTGTAATCCAGCCTTACTTTGGTAATACATACTTGCCCCACTTGTATTTAGTTAATGGTGGCACTAGCTAGATAGTAATGTTAAATGATGATAAGCAGTAGTGATTCATCATTCAAATGTATCATTGTCCTTTAATGTTAAGCGCAAATAGATTTTCATTGTTCTCCCATGTGCTTCATGTTTTATGTATTTATAGGTAGGTACTTAATGTTTTATAAATATTTTTTTGTTAATTGGGAATCCCCAGTCCCCATTGTCTGGACCAGTTTATATATAATTGAACTAACAAGAGTGTGCTTTAAATACTATTCTCTGCAATTATGATAATTAAACAACATGAATTTCTCTTCACTTCCCTTCTCTTATTTAAATAATATTGTAGGAAACTGTAATAACTAATACAAGATTATAAATTTCATTCTAGCAACTGGTGATGTAATCCATGTGGTAAATTCCAAAGATGTGCAGGTCGGCCATCAGTATGTGTACAACATGGGAACTCCCGGAGCTAACTCACAGAAGAATAACCCATTTGATGATGAAGAAACAGTAGAAAAGACAAATCTAATAACTCTGGTCATGGAAGCAAAAATTATGGTAATAACACATTTTTAACTAGGCATAAGGTCATAATTTAGCCAGAAT CATCAGCTTGT> P. xylostella- Cactus (PxCac); Px016665 (SEQ ID NO: 122)CAATGCTGCGGAGGGTCTATGCGGGTGGACACCTCTACACGTAGCGGCGGCGCGAGGCGACGTCGACACGGCTCGCTACTTGCTCGAGAAGTGCGCTGGCGTCGATCCCTCTGCCCTGGACTACGCCGGTCGTACGGCCAGGAAACTGGCGTTGAAGAATAAAGCGGCCGCCCTGTTTGACGGCAGTGAGGGCAGCGAGGAGGAGGATAGTGACAGTGAGGATGAGATGCTTCTGGAAAGCGACCAGAGTCTGTTCGACCGGATCCGTGACGGTATGAACGCCATCAACGTCGCCTGA> P. xylostella- Dorsal (PxDor); Px000110 (SEQ ID NO: 123)TAACCTGTGCCAGCAACCAATGGCTCCTATGGCGCAGCAGCTGATGGACCCGTCCCCCAGCGACCCACCCTCCATCACGGGGCTGCTGATGGATCGCCCGGACCAGCCCTACTCCGGGGAGCTGTCTGGACTCTCCGCCCTGCTGGCTGAGGCAGCCCCCGCAGAGATGCTCAGCGATAGCCTCAACAGACTGTCTACGGGGGACTTGTTGAGACAAGTTGATATGTGA> P. xylostella- Beta 1-3 glucan recognition protein 2 (PxβGRP2); Q8MU95.1(SEQ ID NO: 124)GAAGGATTGAAGTGAGCGCCAGAATGCCGCGCGGTGATTGGTTGATTCCAGATATTCTGCTGGAGCCGAAAGAAAACCTTTACGGAGTACGCAATTACGCGTCAGGTCTACTCAGCATAGCCTCAGTCAGAGGAAACACTGCTTACTCGAAGACCCTCAAAGGAGGCCCCATACTGTGTGACAAGGAACCGCAGAGAAGTGCCAAGTTGAGCGAAAAAGTTGGATATGACCATTGGAATAAAGCCTTCCATAACTACACCATGATTTGGGCACCAAGTGGCATCACCATGCTGGTGGACGGCGAGCAGTACGGGGACATCCGTCCCGGCGACGGCTTCAGCCAGGACCCGGCGGTGAGCAGCGTGGTGGCCGCGCCGCAGTGGCTGAAGGGCACCAGCATGGCGCCCTTTGATGTTATGTTCTACATATCCCTTGGTCTCCGCGTGGGCGGAGTGAACGACTTCCCCGACACTCCTGAGAAGCCGTGGAAGAACAAGGCCACTAAAGCCATGCTGAATTTCTGGAACGCCCGGGAACAGTGGCAGAGCAGCTGGTTTGAGGACACCACTGCACTCCTCATAGACTATGTCAGGGTTTATG CGCTGTGA> P. xylostella- Chitin synthase 1 (PxCHS1); KX420688.1 (SEQ ID NO: 125)CGTATCTCGCACGACCTGAAAGAGCTGCGAAACTCATCCGTCTTTTCCTTCTTTATGATCAATGCCCTCTTTGTTCTCATCGTATTCTTGCTGCAACTGAACAAGGACAACCTCCACATAAAGTGGCCCTTCGGAGTCAAAACTAACATTACGTATGATGAGGTGACGCAAGAGGTGCTGATCTCCAAGGATACCTGCAACTAGAGCCTATTGGTCTGGTGTTCGTGTTCTTTTTCGCATTGATTTTAGTCATCCAGTTCACTGCCATGTTGTTCCATCGATTCGGAACTTTGTCGCATATATTATCGTCTACGGAACTGAACTGGTTCTGCAATAAGAAGGCGGAAGACTTATCTCAAGACGCACTGCTAGATAAGAATGCGATAGCAATAGTGAAGGATCTCCAGAAACTAAACGGGCTCGATGACGGGTATGACAATGACTCGGGGTCGGGCCCGCACAATGTGGGAAGGAGAAAGACGATACACAACCTGGAGAAAGCGAGACAGAAGAAGAGGAACATAGGAACGCTCGACGTCGCTTTCAAGAAGCGATTCTTCAACATGAACGCTAATGAAGGACCAGGAACACCAGTTCTGAACCGCAAGATGACGTTGCGAAGAGAGACGTTGAAGGCGTTGGAAACGAGGAGGAATTCTGTGATGGCCGAACGAAGGAAGTCGCAAATGCAAACACTTGGAGCTAACAACGAATATGGAGTCACTGGAATCTTAAACAACAACCCAGCGGTGATGCCGCGCCACCGGCCGTCGACAGCCAACATTTCGGTCAAGGACGTCTTCGCGGAACCCAACGGGGGACAAGTGAACCGAGGGTACGAGACCACGCACGGCGACGAGGGAGACGGCAACTCCATCAGACTGCAGCCGAGAACCAACCAGGTCTCCTTCCAGGGGAGATACCAATAA> P. xylostella- Beta tubulin (PxβTUB); KX420688.1 (SEQ ID NO: 126)GAAGGAGGTCGACGAGCAAATGTTGAACATCCAGAACAAGAACAGCAGCTACTTCGTCGAATGGATCCCGAACAACGTCAAAACGGCCGTGTGCGACATACCGCCTCGTGGACTGAAGATGTCTGCCACCTTTATCGGGAACACGACAGCAATCCAAGAGCTCTTCAAGAGGATTTCTGAGCAGTTCACTGCTATGTTCAGGAGGGAAGCGTTCCTCCACTGGTATACTGGTGAAGGCATGGACGAGATGGAGTTCACAGAGGCGGAGAGCAACATGAACGACCTGGTCTCCGAGTACCAGCAGTACCAGGACGCCACGGCTGAAGACGAGGGAGAATTCGACGAGGATATTGAAGACGA GTGA> S. frugiperda-Peptidoglycan recognition protein 1 (SfPGRP1); rep_c7951(SEQ ID NO: 127)CCTGATTGGTGGAAACGGGAGAGTTTATGAAGGAGCCGGCTGGCATCACGTTGGGGCCCATACTTTGGGATACAATGCAAGATCTGTGGGGATCTCCTTCATTGGCGATTTTAGAACAAAATTACCAACACCCGAAGCACTGAAAGCCTTCAACAGTCTCCTGGAATGTGGAGTCACGAACAATTATCTGTCAAAGGACTATCACCTGGTGGCCCATAGTCAGCTCTCTATGACTGACAGTCCYGGAGACATGYTGAGGAAGCAGGTGGAATCGTGGCCTCMTTGGCTGGATAATGCCAAAGACATACTTAAGTAGAARAAGACTAAACGCCGTACTTTGAGCCATTTAATGGTTACTTAACCCAGTCCTTAGCAATTTGATACAAGGCCAATGTCTCTAAGGGCGGCAGTAAAGGTCAAAACACATTTAATGAGTGTGTTTAAGATTTTGCTAGTGAAAATTGTTTTGAAGTACGTATTTGATGTAAGTGATGATATCAGTACCCTTAGTATGAGTTTGCTTTACGTTCCACGAGATGGAAACGAGAGCGCGTTCGGCGCTCTGATTGGTTCGTTCATTCATGCCGGCC> S. frugiperda-Attacin (SfAtta); rep_c9395 (SEQ ID NO: 128)GCCAGACATCTACCACAGGACCACTCAACGTACGACCAAGTACAACTCCTCGGGTTCGACGAAGATGGACGACCAGTGTTTGAGCACGAAGACTTACTCCCAGAACTAGAGGAGTCCTACCAGCCAGAGCACCTGGCGAGGACTCGCAGACAGGCGCAGGGCAGCGTCACCCTCAACTCCGACGGCGGCATAGGCCTGGGCGCTAAGATCCCGCTCGCACACAACGACAAGAATGTGGTGAGCGCCATCGGCTCCATGGACTTCAACAACAAGTTGCAGCCTGCTTCCAAGGGCTTCGGTCTGGCTCTGGACAACGTCAACGGGCACGGACTGACGGTGATGAAGGAAAGTATCCCCGGGTTCGGGGACAGGCTGTCGGGCGCTGGCAAGCTGAACGTGTTCCACAACGACAACCACAACGTGGCCGTGACCGGCTCTCTCGCCAGGAACATGCCCAGCATCCCGAACGTGCCCAACTTCAACACGTACGGCGGGGGCGTCGACTACATGTACAAGAACAAGGTGGGAGCGTCTCTGGGCATGGCCAGTACTCCGTTCTTGGACCGCAAGGACTACTCCGCGATGGGCAACCTGAACCTGTTCCGCAGCCCGACCACTACCGTGGACTTCAGCGGCGGCTTTAAGAAGTTCGAATCTCCCTTCATGAGCAGCGGCTGGAAGCCTAACTTCGGCCTTACTTTCGGCAGATCTTTCTAGATATATTTTGTAATCTAAATTTAACTTTAACTTTGTTGTATAATATTTTGTCGAATTAAGATCAGTATTGTTCATACTAATATTATATTATCAGTGTTTCTTATAAATTAA> S. frugiperda-Hemolymph proteinase 10 (SfHP10); c12881(SEQ ID NO: 129)CAAGGCTCGTACCTTGCAGTTGAACCGACAATCTATTCCTAAAGCCTTTTTAAGGTCAGGAAAAATAGTTCCTACATCTAAATGCAGTAGAATTTGCGAAACGAATTTAAATAAAAATGGCGTCGATTGTGTTTGTGATTTTGTGTGTTACCGTCGCTGCGGTGAAAAGCGCGATTTTAAACCCGTGGAGTAAAGTTGAGGCCAACAAATGTGGTGTAGAAGCCAGTACTAACTTGGTCCATCACAATCCATGGTTGGTCTACATCGAGTATTGGCGTGGAAACTCAGATACTGAGATCCGATGCGCCGGTACTTTAATCGACAGCAAACATGTCGTCACAGCTGCCCACTGCGTTAGGACTCTGAAGTTTAGTCATTTGATCGCCCGTCTTGGCGAATACGACGTAAATTCTAAGGAGGACTGCGTTCAGGGCGTGTGTGCCGATCCCATCGTCAGAATCAAGGTGGCTGAGATCATCGTGCATCCTAACTACAGCAACCGGGAACA> S. frugiperda-Trypsin like serine protease (SfTSP); rep_c48453(SEQ ID NO: 130)AGCAACAAAATGCGTGTCCTCGCTTGCTTGGCCCTTCTCTTAGCTGTGGTAGCAGCCGTCCCCTCCAATCCCCAGAGGATTGTGGGTGGTTCGGTCACCACCATTGACCGGTACCCCACCATTGCATCCCTGCTGTACTCGTGGAACTTGAGTTCCTACTGGCAGGCGTGCGGTGGTTCCATCTTGAACAACCGTGCCATCCTTACTGCTGCCCACTGCACAGTTGGTGACGCCGCCAACAGATGGAGAATCCGTCTTGGCTCCACCTGGGCCAACAGCGGTGGTGTCGTTCACAACGTCAACACTAACATCGTCCACCCCTCATACAACTCTGCAACTTTGAACAACGACATCGCTATCCTCCGCTCCGCCACCACCTTCTCCTTCAACAACAATGTTCAGGCTGCCTCCATTGCTGGTGCCAACTACTTGCCCGGTGACAACACCGCCGCCTGGGCCGCTGGATGGGGAACTACCTCCGCTGGTGGCTCTAGCTCTGAGCAGCTCCATCACGTTGAGCTGCGCATCATCAACCAGGCTACTTGCAAAAACAATTACGCTACCCGCGGTATCACCATCACCTACAACATGTTGTGCTCTGGCTGGCCCACCGGTGGTCGCGACCAGTGCCAGGGTGACTCTGGTGGTCCTCTCTACCACAACGGCATCGTTGTTGGTGTCTGCTCTTTCGGTATTGGCTGTGCTCAG> S. frugiperda- C Type Lectin 6 (SfCTL6); Joint2_ rep_c448(SEQ ID NO: 131)AACAGTTTTCTATTGGCAGTCAAAGACTTCAGTCGAAAAATAATCCTCATCAGAGTCGTGAAGCAAGGTGCCCAAAATATAATGTAACCTAGATACCTATTAATAAATTATTTGTCAACCAAAACGTTACGTTCAAAGTCCTTAAAATCAAAATATCTTATGATTAGTTTTGATTTAAAAATAGAGGTTCGAAATCGCCAACCCAAATAGGTTTAGTTTACGATTCAGGAAAAATCCTAACGTAGGGAAACATTATTTTACAAGACTTTTGGCTTAAAAACTTTGAGAACCAATGTCAAATTTGATAATAACTAATGAGGTATAAAAGCTTGATCCTATTAGGACTTATTTTCATAACACCATCGAGTTTGTATTTAATRRAGACGTGKGTTAACTAACAACATGAAGACCGGTGTAAAATATTCTGTTMTTTGGATATTCTCTCTATTYTGCTATATAGAGGCAACATTTCGTTGTGACTACACGTACAGCAAGGAAGCGAAGGGCTGGTTCAAACATGTGGTGATACCAGCTACTTGGGCTGACGCACGWCTGCACTGCACGTTGGAAGGTGCAACGCTGGCTTCTCCACTCAACCAGGCTATMAGTAATGAGATGCAGTCCMTCCTGGCRAACCTCTCGGCGCTGCAATCAGAAGTCTTCACTGGAATTCACRCGACTKTTTCACGRMRCAACTTATATCATACYATYGAAGGTATACCTCTTAGTAAAATTCCATTAGATTGGGCAACAAATGAGCCAAATGGTGGGAGAGATGAAAACTGTATCACGTTTAACTCCGATGGCCAAGCGGCAGACAGATCCTGTAARGAGACTCGACCTTACATCTGCTACCGACACACWACTAAAGTGACTGTGKCCAATGAATGTGGGACTGTAGATCCTGAATACAATTTGGATAAAAGAACGGGCKCYTGCTATAAGTTCCACACRGTACCTCGCACGTTCGAGCGTGCCAACTTCGCGTGTTCTGCTGAAGGTG> S. frugiperda- Cecropin (SfCec); rep_c42380 (SEQ ID NO: 132)TTCGTGTCGTATCACTAGAGTTCGAAATACAAAATAATAATACATTTATTATTTTGCCATAATTAATAATAAAGTTATTTTATTTCATAATAATAATGAATTTCACAAAGATATTTTGTTTGTTTTTGTCTTGCTTTGTTTTGATGGCGACCGTGTCAGGAGCTCCTGAACCGAGGTGGAAATTCTTCAAGAAAGTGGAGAAGTTGGGCCAAAACATCCGCKATGGTATCATAAAGGCAGGACCCGCAGTGGCCGTGGTGGGATCAGCRGCAGCCATWGGAAAGTGAKCCCTACGACCTGAGACATGAAGACTAATATCCAYTAAAATAASAATATTGAGGCKTATAATATTAATTTATTRTRTTTGTAAATTAAATTATTTGTAAGATAA> S. frugiperda- Beta 1, 3 glucanase recognition protein (SfβGRP2);EF641300 (SEQ ID NO: 133)GCAACAATCGCACCAACTCTTTCGTGCGCAGTGGAAGTTTGTTCATCCGTCCCTCTCTAACATCAGACGAGTTCGGAGAAGCTTTCCTCTCATCTGGACACTGGAACGTCGAGGGTGGTGCTCCTGCTGATAGATGCACAAATCCACAATGGTGGGGTTGCGAGAGAACAGGCACGCCGACCAACATTTTGAACCCAATCAAGAGTGCTCGTGTCCGTACCGTCAATTCCTTCAGCTTCCGTTACGGACGCCTCGAAGTCCGCGCTAAAATGCCCGCCGGAGATTGGATTTGGCCAGCTATCTGGTTGATGCCTGCGTACAACACTTACGGTACTTGGCCCGCATCAGGAGAGATTGACTTAGTTGAGTCCCGAGGCAACCGTAACATGTTCCACAATGGTGTCCATATCGGTACACAGGAAGCAGGCTCGACCTTGCACTACGGACCTTACCCAGCGATGAACGGTTGGGAGCGCGCCCATTGGGTCAGAAGGAACCCT> S. frugiperda- joint2_rep_c16438 (Sfrc16438); Un-annotated(SEQ ID NO: 134)TCGTTCTCCTCGTCGCTTTCTTGGGGACCTCATGGTTTACGGGAGATGTTTCTGCGAGTCCGCGGCCGCAAGAGCCGCGTGTGGATCAAAATCCGAATCAGGTGTCACCTTATGGAGGGTCCGGGTACCACGCACCTCCGCAGTACCAACCGCAGTACCAACCACAGCCGTACTACCCACAGCCGCAGTACTACCCACAGCCGTACTACCCACCTCCTCAGTATTACCCACCGCAGCCACAAACACCTGAGAATGCTCCACTCATAAACACATGGAATGGTTTCCACGACTGGGCTCAGAATATCGTTCAAAGTGCTTTGGGGCAGAAATTCCCGAAAGGTAGACAGTAACTTTTTAATTGTCAATTGAAGATAAGGCCCATTTCACCAACTGCTGTTTAATTTTAAGGAGCTCCTAAACTAACATAGGTGACACTTAGCGATATTCTGGATTTTTTGTGAACGTATAAATAATATCCAAATGTAAAAGATAAGAGGCCAAGAA> S. frugiperda- Chitin synthase B (SfChsB); AY52599) (SEQ ID NO: 135)GAATTTAGGAGCAGCGTGCGGGCGCATCCATCCTGTGGGCTCAGGCTTCATGGCATGGTATCAAATGTTCGAGTACGCTATTGGTCATTGGCTGCAAAAGGCGACTGAACACATGATTGGCTGTGTACTCTGTAGCCCTGGATGCTTCTCCCTCTTCAGAGGAAAGGCTTTGATGGACGACAACGTTATGAAGAAATATACCTTAACTTCCCACGAGGCACGACACTATGTGCAATACGATCAAGGCGAGGACCGTTGGTGCACGCTACTGCTGCAGCGCGGGTACCGCGTGGAGTACAGCGCGGTGTCGGACGCGTACACGCACTGCCCCGAGCACTTCGACGAGTTCTTCAACCAGCGCCGCCGCTGGGTGCCCTCCACGCTCGCCAACATCTTCGACCTGCTCGGCAGCGCCAAGCTCACCGTCAAGTCCAACGACAACATCTCCACCCTCTATATAGTCTATCAGTTCATGTTGATAGTGGGTACGGTGTTGGGTCCCGGCACGATCTTCCTGATGATGGGGGGAGCCATGAACGCCATCATTCAGATCAGCAACGCGTACGCGATGATGTTGAACCTCGTACCACTCGTCATCTTCCTTATAGTCTGTATGACTTGTCAGTCAAAGACGCAGCTCTTCCTCGCTAACCTCATAACATGCGCATACGCAATGGTGATGATGATCGTGATAGTGGGGATAGTTCTGCAGATAGTGGAGGATGGATGGCTGGCTCCGTCCAGTATGTTCACAGCTTTAATATTCGGTACATTCTTCGTCACCGCGGCACTACACCCGCAAGAGATCAAATGTTTGTTGTTCATAGCAGTGTACTATGTAACCATCCCTAGTATGTACATGTTGTTGATCATATACTCCATCTGTAATCTCAACAACGTATCCTGGGGTACCAGGGAGACACCGCAGAAGAAAACTGCTAAGGAAATG> T. castaneum- Peptidoglycan recognition protein 2 (TcPGRP2);XM_965754.3 (SEQ ID NO: 136)ATGAGTGGCAGTGACCCTTTAACAAATACCCAACAATCCGATCAAGATTATTACCATCCACTCTGTTATTCAATTCAAGTGGACGACGAAAATGAACAATCAGCTCTCCTGCCCGCATTTCATCAAAGGAAAAGTTTGCGAGTTCAGGATAAAATCTTTATTGTATTTTTATTTTCAATTCTAATTACCGGACTAGCCATTGGCCTCTATCTCCTTGCAACTGAGGGACACGAATGGAAAGCTGCAGGAGTCTATAATATTACAGTTCGGGAACAGTGGCAAGCTCACGTCCCTTCATCAACAATGCCAAAGTTGGAACTTCCCGTAAGAAGAGTTTTATTTCTTCCTGCAAATACCACTAGCTGCGGCAGCAAATCCCACTGTGCCAAAGTCCTCCAGGAACTACAATTACAGCATATGCTGCAGTGGAAAGAACCTGACATCTCCTACAATTTCATAATGACTGCAGATGGCAGAATTTTCGAGGGGAGAGGATGGGACTTTGAAACTTCTGTTCAAAATTGTACGGTTAATGATACTGTGACAGTTGCTTTTTTGGACGAATTAGATGCGAAAGCACCGACGTTTAGACAAGCTGAAGCGGCAAAAATGTTCCTGGAAGT> T. castaneum- Beta 1-3 glucan binding protein (TcβGRP2); XM_966587.4(SEQ ID NO: 137)ACATTACAACGTTCCGCGACCCTCAATCCAAGCATTCAGGCCCCGTGGCTTCAAAGTGAGCATCCCTCATACTGAAGGCATCCAATTATTCGCCTTTCATGGAAATATTAATAAACCCCTGCACGGTCTCGAGGCCGGACAATTTTCCCAAGACGTCCTCCAAAGGGAGGGAGACGAGTGGGTGTTCCAAGACTCCAGTGCCAAATTAAACGTGGGAGATAAAATCTATTATTGGCTCTTTATCATTAAAGAAGACCTGGGCTACAGATACGATCACGGCGAGTACGAAGTGAAAGTTTTAGCCACCCGTGACTTCGATTCTCCTCAAACAACCTCTGTGACGCCGAATCTTGCCCCCAATCTCGGCATTTGCGAAAAGGTGATGGTAAATCTCACGCGGAAGCTGCTCGATTTGCAGCAAGAAATTGAGTCCCTTAGGGAGACGAACGATATTTTGGAGGATATGGTTCAGAAGCACACTGATACGGCGACTACTCTCACTTTGGACGGCTTGATGATAAAGGATGACGACGAACTTGTTTCGGTGATTCAAGCAATTATTAAAGATAAACTTGGACTAAAAAGCAGGATTCAAAATGTGACGAGGCAGGAGAATGGAATGGTCAAGTTTCAAGTGGCGAGTTTGAGAGAAAAGTTGGAGGTTGTCAAAGCGCCCAAAAGAAAACTCAAGTCGTCGAGCTTTACGATCACGTATTAA> T. castaneum- midgut protein (TcMDGP); XM_971351 (SEQ ID NO: 138)CCGTTGAAGAGGATGCCCAGTGAAGAAATCAGTATCAGTGATCTGCCTAGCGAAATGAAAGAAGTTTTACTAGAAATTAGCCCGAACTTTGATGAAAATCTGAAACGGGCTTTCAGGAACGAAGGAGTGAGGCTGCAGAGAGTGCAGAACAATGGACGATTTATTCATCAGCTGGACGACGTTCTCTTATCCATAGACGATACCAAAATCGAGTTACGCAACCTGAAATTCCCCTGGATCCCCGACTTCCGCATCGTGGACTTGTCCAGCGACCTGCCCATGTCATGCCTCGACCTAAACCTAAACCTGGGCAATTTGCGAATTGAGGGCGAGTACGAAGCCAACAACACCACACTCAGGCGATGGCTCCCGGTATCTCACATTGGTCGAATCGTGATCGGTTTTAACAACGTCCGAGCGAACGGAAAAGTCGGACTCGTGCTCGAGCAGGATTCTTTCGTTCCGCAGAATTATGATATTAGATATGAGCCGACCGATGTTGTTATTAGGGTTAGCTATCACGTGGATGGCGAGAATGAGGTGCAAAATGAGATTACCAATTCAGATATTGAGGCCACGCTAGGCAAGACCGTGTGGGTGCAGTTGACTGAGATATTGTCCAACCTGTTGCATAGGCAATTGGGCGAGGTTGTAGTGGAG T> T. castaneum- Chitin synthase 2 (TcCHS2); EFA 10719.1 (SEQ ID NO: 139)ATGGCGGCGCGTCATCGCTTTGCCACAGGGAGCCCTGAGGAAACAGAGCCCCTGTATTCGTCGACGCAAATGCCCGAAAAAGTCCGGGAAAAATGGAACGTCTTCGACGACCCCCCAAGAGAGCCCACTTCGGTTCCGAAGTCAAAAGAACCTACATCGAGTGGGGGGTGAAGTTTTTGAAAGTTGTGACAATCATAACTGTGTTTTTTGTGGTCCTTGGTGCTGCAGTGGTTTCGAAAGGGACAACCTTGTTCATGACGTCACAAATAAAAAAGAATGTGACAAGGGCTTATTGCAACAAAAAGATAGACCGCAACCTCCAATTCGTCGTCTCCCTCCCCGAAGTGGAGCGCGTGCAATGGATCTGGCTCCTCATTTTCGCTTACTTGATCCCCGAAGTGGGTACCTGGATCCGCGCCGTCCGCAAATGCCTCTACAAGCTCTGGAAAATGCCCTCCCTCTCCGAATTCCTCTCCCTCTTCGGCACGGAAACGTGCCCCGCCATCGGAAGCGCAATTTTGATATTCGTCGTCCTCCCCGAGCTGGACGTCGTCAAAGGGGCGATGCTCACAAACGCGGTTTGTTTCGTGCCCGGAGTTGTGGCAATGTTCTCGCGCAAACCGTGCTCCATAAACGAGAACCTGAAAATGGGGCTGGACATCGCCAGCATAACTGCACAGGCGTCAAGCTTCGTGGTGTGGCCCTTGGTTGAAAATAACCCGACCTTGTACCTAATCCCCGTTTCCGTGATTTTGATTTCGGTGGGT *****

The breadth and scope of the present disclosure should not be limited byany of the above-described exemplary embodiments, but should be definedonly in accordance with the following claims and their equivalents.

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1. An isolated double stranded RNA (dsRNA) molecule comprising a nucleicacid sequence complementary to about 21 to 2000 contiguous nucleotidesof a target gene sequence, optionally about 21 to 100, about 21 to 200,about 21 to 600, or about 21 to 1000 contiguous nucleotides of a targetgene sequence, wherein the target gene sequence includes at least one ofthe protein coding region, the 5′ UTR region, the 3′ UTR region, and anycombination thereof, of a target gene and optionally wherein the nucleicacid sequence is complementary to a contiguous region comprising atleast about 50% of the length of the target gene sequence protein codingregion, 5′ UTR region, or 3′ UTR region, and wherein the double strandedRNA molecule silences the target gene when ingested by an insect, andwherein (a) the target gene is a MIGGS-IRTG involved in gut microberecognition, clearance and/or containment induced by microbes ingestedduring feeding and/or active feeding, or (b) the target gene comprises anucleic acid sequence selected from the group consisting of SEQ ID NOs:1-14, 16-29, 31-70, 71-75, 76-88, 89-105, and 106-110.
 2. The isolateddsRNA molecule of claim 1, wherein the target gene is a MIGGS-IRTGinvolved in gut microbe recognition, clearance and/or containmentinduced by microbes ingested during feeding and/or active feeding. 3.The isolated dsRNA molecule of claim 2, wherein the dsRNA moleculecomprises a single RNA strand comprising an inversely repeated sequencewith a spacer in between and wherein the single RNA strand can anneal toitself to form a hairpin loop structure; or wherein the dsRNA moleculecomprises two separate complementary RNA stands annealed together. 4-7.(canceled)
 8. The isolated dsRNA molecule of claim 2, wherein the targetgene is: (a) a type 1 MIGGS RNAi target or a type 2 MIGGS RNAi target;or (b) a pattern recognition receptor (PRR) class gene or an insectmidgut structural component gene; optionally wherein the target gene isexpressed abundantly in a midgut specific manner during active feeding.9-10. (canceled)
 11. The isolated dsRNA molecule of claim 2, wherein thetarget gene is selected from the group consisting of M. sexta-Hemolin(MsHEM), M. sexta-Serine proteinase homolog 3 (MsSPH-3), M.sexta-Peptidoglycan recognition protein 2 (MsPGRP2), M. sexta-Beta-1,3-glycan-recognition protein 2 (MsβGRP2), M. sexta-Relish family protein2A (MsREL2A), M. sexta-Dorsal (MsDor), M. sexta-Spätzle (MsSPZ1A), M.sexta-Toll receptor (MsTOLL), M. sexta-Scolexin A (MsSCA1), M.sexta-Hemolymph proteinase 18 (MsHP18), M. sexta-Transferrin (MsTRN), M.sexta-Arylphorin beta subunit (MsARP), M. sexta-Chymotrypsinogen-likeprotein 1 (MsCTL1), M. sexta-Valine Rich Midgut Protein (MsVMP1), M.sexta-Imd (MsImd), M. sexta-FADD (MsFADD), M. sexta-Dredd (MsDRD), M.sexta-Relish F (MsReIF), M. sexta-Cdc42 (MsCdc42), M. sexta-Dsor1(MsDsor1), M. sexta-Fos (MsFos), M. sexta-Jra (MsJra), M. sexta-Caudal(MsCAD1), M. sexta-Atg8 (MsAtg8), M. sexta-Atg13 (MsAtg13), M.sexta-IAP1 (MsIAP1), M. sexta-Chitin synthase 2 (MsChs2), M.sexta-Beta-1 tubulin (MsβTub), M. sexta-Beta fructofuranosidase 1(MsSuc1), and orthologs thereof.
 12. (canceled)
 13. The isolated dsRNAmolecule of claim 1, wherein the target gene comprises a nucleic acidsequence selected from the group consisting of SEQ ID NOs: 1-14, 16-29,31-70, 71-75, 76-88, 89-105, and 106-110.
 14. The isolated dsRNAmolecule of claim 13, wherein the target gene sequence selected from thegroup consisting of: i) SEQ ID NOs: 1-9, 11, 14, 31, 39, 43, 44, and71-75; ii) SEQ ID NOs: 3, 4, and 43, wherein the dsRNA molecule causesimpeded growth, developmental progression, and/or mortality and the likeof TH, DMB, and FAW in an orthologous manner; iii) SEQ ID NOs: 76, 77,80, 81, 85, 87, and 88, wherein the dsRNA molecule causes impededgrowth, developmental progression, and/or mortality and the like of DBM,optionally wherein the DBM is a Bt resistant strain; iv) SEQ ID NOs: 89,92, 96, 101, 103, and 105, wherein the dsRNA molecule causes impededgrowth, developmental progression, and/or mortality and the like of FAW;and v) SEQ ID NOs: 107-110, wherein the dsRNA molecule caused impededgrowth, developmental progression, and/or mortality and the like of RFB.15. (canceled)
 16. The isolated dsRNA molecule of claim 13, wherein thedsRNA molecule comprises a single RNA strand comprising an inverselyrepeated sequence with a spacer in between and where the single RNAstrand can anneal to itself to form a hairpin loop structure; or whereinthe dsRNA molecule comprises two separate complementary RNA standsannealed together. 17-19. (canceled)
 20. The isolated dsRNA molecule ofclaim 1, comprising a nucleic acid sequence selected from the groupconsisting of SEQ ID NOs: 111-119, 120-126, 127-135, and 136-139, or afragment of at least about 21 nucleotides thereof; optionally, wherein:i) the isolated dsRNA molecule comprising a nucleic acid sequenceselected from the group consisting of SEQ ID NOs: 110-119, or thefragment thereof, causes impeded growth, developmental progression,and/or mortality and the like of TH; ii) the isolated dsRNA moleculecomprising a nucleic acid sequence selected from the group consisting ofSEQ ID NOs: 120-126, or the fragment thereof, causes impeded growth,developmental progression, and/or mortality and the like of DBM; iii)the isolated dsRNA molecule comprising a nucleic acid sequence selectedfrom the group consisting of SEQ ID NOs: 127-135, or the fragmentthereof, causes impeded growth, developmental progression, and/ormortality and the like of FAW; or iv) the isolated dsRNA moleculecomprising a nucleic acid sequence selected from the group consisting ofSEQ ID NOs: 136-139, or the fragment thereof, causes impeded growth,developmental progression, and/or mortality and the like of RFB.
 21. Theisolated dsRNA molecule of claim 1, wherein the dsRNA molecule can formsiRNA.
 22. An isolated siRNA molecule derived from the processing of thedsRNA molecule of claim
 1. 23. An insecticidal composition comprisingthe isolated dsRNA molecule of claim 1 or an siRNA produced therefromand a synthetic carrier or microbial conduit that can be a microorganismthat has a natural capacity or is engineered to produce and/or deliverdsRNA to increase its bioavailability and/or biostability for causingRNA interference including but not restricted to plant growth promotingorganisms, normal commensal and/or symbiotic microorganisms associatedwith the target insect pest or parasites and/or natural enemies of thetarget pest or pest target host or host cultivation range etc. from aninsect or parasite and/or natural enemies of the target pest engineeredor identified from natural populations containing microbial conduit toproduce and/or deliver dsRNA and/or drive the transmission of suchmicrobial conduits into natural populations of insect pests as a controloption, and optionally wherein the dsRNA molecule is bound to thesynthetic carrier when present.
 24. (canceled)
 25. A recombinant DNAconstruct encoding a dsRNA molecule of claim
 1. 26-40. (canceled) 41.The recombinant DNA construct of claim 25, wherein the gene silencingsequence is operably linked to one or more promoters for the expressionof a dsRNA molecule that silences the target gene when ingested by aninsect, optionally wherein the recombinant DNA construct furthercomprises an additional transcription regulatory region or an additionaltranscription regulatory element.
 42. The recombinant DNA construct ofclaim 25, wherein the construct is an expression vector and theexpression vector can target single or multiple insect RNAi target genesor chimeric RNAi target genes.
 43. (canceled)
 44. A host cell comprisingthe dsRNA molecule of claim 1, optionally wherein the host cellexpresses the dsRNA and/or produces a siRNA therefrom.
 45. The host cellof claim 44, wherein the cell is a bacterial cell, a yeast cell, afungal cell or a plant cell. 46-47. (canceled)
 48. A transgenic and/ortransplastomic plant comprising the dsRNA molecule of claim 1, or aseed, part, tissue, cell, or organelle thereof wherein said seed, parttissue, cell or organelle comprises the dsRNA; optionally wherein atleast one cell of the plant expresses the dsRNA molecule and/or producesan siRNA molecule therefrom. 49-50. (canceled)
 51. A method ofsilencing: (i) an insect immune response gene and/or (ii) an insect geneencoding for structural components of the insect midgut, the methodcomprising providing for ingesting the isolated dsRNA molecule of claim1 or an siRNA molecule, optionally wherein the dsRNA is ingested by theactively feeding stage of the insect and (a) ingestion of the dsRNAinduces a melanotic response; and/or (b) ingestion of the dsRNA resultsin perturbation of gut microbial homeostasis; and/or (c) ingestion ofthe dsRNA results in defective clearance of opportunistic microbes;and/or (d) ingestion of the dsRNA results in defective containment ofgut microbes.
 52. A method of protecting a plant from an insect pest ofthe plant, the method comprising topically applying to the plant theisolated dsRNA molecule of claim 1, and providing the plant in the dietof the insect pest, optionally wherein said dsRNA is topically appliedby expressing the dsRNA in a microbe and topically applying the microbeonto the plant, optionally wherein the dsRNA is ingested by the activelyfeeding stage of the insect and (a) ingestion of the dsRNA induces amelanotic response; and/or (b) ingestion of the dsRNA results inperturbation of gut microbial homeostasis; and/or (c) ingestion of thedsRNA results in defective clearance of opportunistic microbes; and/or(d) ingestion of the dsRNA results in defective containment of gutmicrobes.
 53. (canceled)
 54. A method of producing a plant resistance toa pest insect of said plant, the method comprising transforming theplant with a polynucleotide encoding the dsRNA molecule of claim 1,wherein the plant expresses the dsRNA molecule and/or produces an siRNAtherefrom.
 55. A method of improving crop yield, the method comprisinggrowing a population of crop plants transformed with a polynucleotideencoding the dsRNA molecule of claim 1 wherein the plant expresses thedsRNA molecule and produces an siRNA molecule therefrom, and wherein thepopulation of transformed plants produces higher yields in the presenceof pest insect infestation than a control population of untransformedplants.
 56. A method for producing a plant resistant against a pestinsect of said plant, the method comprising: a) transforming a plantcell and/or organelle with a polynucleotide encoding the dsRNA moleculeof claim 1; b) regenerating a plant from the transformed plant celland/or organelle; and c) growing the transformed plant under conditionssuitable for the expression of said double stranded RNA molecule,wherein said transformed plant of (c) is resistant to the plant pestinsect compared to an untransformed plant. 57-61. (canceled)
 62. Theisolated dsRNA molecule of claim 1, wherein the silencing of the targetgene results in reduced appetite and/or developmental defects resultingin incomplete development and/or mortality and/or decrease thereproductive success of the insect, optionally wherein the reducedappetite and/or developmental defects and/or mortality and/or reducedreproductive fitness of the insect is observed after sustained feedingfor at least 24 hours.
 63. (canceled)
 64. The isolated dsRNA molecule ofclaim 1, wherein the insect is of the order Lepidoptera, Coleoptera,Hemiptera, Blattodea, or Diptera.
 65. The isolated dsRNA molecule ofclaim 1, wherein the insect is Manduca sexta (M. sexta) (tobaccohornworm), Spodoptera frugiperda (fall armyworm), Ostrinia nubilalis(European corn borer), Plutella xylostella (Diamondback moth),Leptinotarsa decemlineata Say (Colorado potato beetle), Diabrotica spp.(Corn rootworm complex), Tribolium castaneum (Red flour beetle),Popillia japonica (Japanese beetle), Agrilus planipennis (Emerald ashborer), Diaphorina citri (Asian citrus psyllid), Cimex lectularius (Bedbug), a cockroach or termite, or insect pests such as mosquitoes andflies.
 66. The isolated dsRNA molecule of claim 1, wherein the planthost is selected from the group consisting of Zea mays L (corn), Sorghumbicolor (sorghum), Setaria italica (fox tail millet), Pennisetum glaucum(Pearl millet), Solanum tuberosum (potato), Oryza sativa (rice),Lycopersicon esculentum (tomato), Solanum melongena (eggplant),cultivars of the Brassica oleracea family, Citrus sinensis (Orange),trees of the Oleaceae family, and crops of Rosaceae.